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Protein

TATA-box-binding protein

Gene

tbp

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II (By similarity). Members of the TBP family are differentially required for transcription and development during early embryogenesis. Regulates mRNA levels in the early embryo by both transcriptional and post-transcriptional mechanisms. Required for transcription of a subset of genes at the mid-blastula transition (MBT). Negatively regulates the expression of other embryonic genes, including autoregulation of the tbp promoter itself. Also functions within a transcription-dependent mechanism to direct the temporally-regulated degradation of a subset of maternal mRNAs after the MBT. This is part of a general mechanism to regulate the maternal to zygotic transition and is required for normal embryonic development. Binds to promoters of a subset of genes. Required for gastrulation.By similarity3 Publications

GO - Molecular functioni

GO - Biological processi

  • DNA-templated transcription, initiation Source: ZFIN
  • gastrulation Source: UniProtKB
  • mRNA catabolic process Source: UniProtKB
  • transcription from RNA polymerase III promoter Source: UniProtKB
  • transcription from RNA polymerase II promoter Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Gastrulation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-DRE-5250924. B-WICH complex positively regulates rRNA expression.
R-DRE-674695. RNA Polymerase II Pre-transcription Events.
R-DRE-6804756. Regulation of TP53 Activity through Phosphorylation.
R-DRE-6807505. RNA polymerase II transcribes snRNA genes.
R-DRE-73762. RNA Polymerase I Transcription Initiation.
R-DRE-73772. RNA Polymerase I Promoter Escape.
R-DRE-73776. RNA Polymerase II Promoter Escape.
R-DRE-73777. RNA Polymerase I Chain Elongation.
R-DRE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-DRE-73863. RNA Polymerase I Transcription Termination.
R-DRE-75953. RNA Polymerase II Transcription Initiation.
R-DRE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-DRE-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-DRE-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-DRE-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Names & Taxonomyi

Protein namesi
Recommended name:
TATA-box-binding proteinImported
Short name:
zTBP1 Publication
Alternative name(s):
TATA sequence-binding proteinBy similarity
TATA-binding factorBy similarity
TATA-box factorBy similarity
Short name:
zfTBF1 Publication
Transcription initiation factor TFIID TBP subunitBy similarity
Gene namesi
Name:tbpImported
ORF Names:si:ch211-202m22.5
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 13

Organism-specific databases

ZFINiZDB-GENE-030616-563. tbp.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003486081 – 302TATA-box-binding proteinAdd BLAST302

Proteomic databases

PaxDbiQ7SXL3.

Expressioni

Tissue specificityi

Enriched in testis but hardly detectable in the ovary (at protein level).1 Publication

Developmental stagei

Expressed both maternally and zygotically. Expression is very low in the zygote, increasing from morula stage onward to reach a peak during early gastrulation. Levels then rapidly decline during neurula and organogenesis stages (at protein level). There is an inverse correlation between mRNA and protein levels at the late blastula and early gastrula stages.1 Publication

Gene expression databases

BgeeiENSDARG00000014994.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000010211.

Structurei

3D structure databases

ProteinModelPortaliQ7SXL3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati128 – 2041Sequence analysisAdd BLAST77
Repeati218 – 2952Sequence analysisAdd BLAST78

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi55 – 87Gln-richSequence analysisAdd BLAST33

Sequence similaritiesi

Belongs to the TBP family.Sequence analysis

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3302. Eukaryota.
COG2101. LUCA.
GeneTreeiENSGT00410000025389.
HOGENOMiHOG000105164.
HOVERGENiHBG044997.
InParanoidiQ7SXL3.
KOiK03120.
OMAiMMPYGSG.
OrthoDBiEOG091G0ENY.
PhylomeDBiQ7SXL3.
TreeFamiTF300102.

Family and domain databases

CDDicd04516. TBP_eukaryotes. 1 hit.
Gene3Di3.30.310.10. 2 hits.
HAMAPiMF_00408. TATA_bind_prot_arch. 1 hit.
InterProiIPR012295. Beta2_adaptin/TBP_C_dom.
IPR000814. TBP.
IPR030491. TBP_CS.
IPR033710. TBP_eukaryotic.
[Graphical view]
PANTHERiPTHR10126. PTHR10126. 1 hit.
PfamiPF00352. TBP. 2 hits.
[Graphical view]
PRINTSiPR00686. TIFACTORIID.
PROSITEiPS00351. TFIID. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7SXL3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQNNSLPPF AQGLASPQGA MTPGLPIFSP MMPYGTGLTP QPVQNSNSLS
60 70 80 90 100
LLEEQQRQQQ QQQAASQQQG GMVGGSGQTP QLYHSTQAVS TTTALPGNTP
110 120 130 140 150
LYTTPLTPMT PITPATPASE SSGIVPQLQN IVSTVNLGCK LDLKTIALRA
160 170 180 190 200
RNAEYNPKRF AAVIMRIREP RTTALIFSSG KMVCTGAKSE EQSRLAARKY
210 220 230 240 250
ARVVQKLGFP AKFLDFKIQN MVGSCDVKFP IRLEGLVLTH QQFSSYEPEL
260 270 280 290 300
FPGLIYRMIK PRIVLLIFVS GKVVLTGAKV RGEIYEAFEN IYPILKGFRK

TS
Length:302
Mass (Da):33,019
Last modified:October 1, 2003 - v1
Checksum:iC63A6C1D2F7E1D50
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti184C → G in AAQ07596 (PubMed:15062100).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY168633 mRNA. Translation: AAO34524.1.
AF503449 mRNA. Translation: AAQ07596.1.
AL772388 Genomic DNA. Translation: CAK04406.1.
BC055549 mRNA. Translation: AAH55549.1.
BC065860 mRNA. Translation: AAH65860.1.
RefSeqiNP_956390.1. NM_200096.1.
UniGeneiDr.117556.

Genome annotation databases

EnsembliENSDART00000016211; ENSDARP00000010211; ENSDARG00000014994.
GeneIDi368882.
KEGGidre:368882.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY168633 mRNA. Translation: AAO34524.1.
AF503449 mRNA. Translation: AAQ07596.1.
AL772388 Genomic DNA. Translation: CAK04406.1.
BC055549 mRNA. Translation: AAH55549.1.
BC065860 mRNA. Translation: AAH65860.1.
RefSeqiNP_956390.1. NM_200096.1.
UniGeneiDr.117556.

3D structure databases

ProteinModelPortaliQ7SXL3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000010211.

Proteomic databases

PaxDbiQ7SXL3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000016211; ENSDARP00000010211; ENSDARG00000014994.
GeneIDi368882.
KEGGidre:368882.

Organism-specific databases

CTDi6908.
ZFINiZDB-GENE-030616-563. tbp.

Phylogenomic databases

eggNOGiKOG3302. Eukaryota.
COG2101. LUCA.
GeneTreeiENSGT00410000025389.
HOGENOMiHOG000105164.
HOVERGENiHBG044997.
InParanoidiQ7SXL3.
KOiK03120.
OMAiMMPYGSG.
OrthoDBiEOG091G0ENY.
PhylomeDBiQ7SXL3.
TreeFamiTF300102.

Enzyme and pathway databases

ReactomeiR-DRE-5250924. B-WICH complex positively regulates rRNA expression.
R-DRE-674695. RNA Polymerase II Pre-transcription Events.
R-DRE-6804756. Regulation of TP53 Activity through Phosphorylation.
R-DRE-6807505. RNA polymerase II transcribes snRNA genes.
R-DRE-73762. RNA Polymerase I Transcription Initiation.
R-DRE-73772. RNA Polymerase I Promoter Escape.
R-DRE-73776. RNA Polymerase II Promoter Escape.
R-DRE-73777. RNA Polymerase I Chain Elongation.
R-DRE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-DRE-73863. RNA Polymerase I Transcription Termination.
R-DRE-75953. RNA Polymerase II Transcription Initiation.
R-DRE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-DRE-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-DRE-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-DRE-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Miscellaneous databases

PROiQ7SXL3.

Gene expression databases

BgeeiENSDARG00000014994.

Family and domain databases

CDDicd04516. TBP_eukaryotes. 1 hit.
Gene3Di3.30.310.10. 2 hits.
HAMAPiMF_00408. TATA_bind_prot_arch. 1 hit.
InterProiIPR012295. Beta2_adaptin/TBP_C_dom.
IPR000814. TBP.
IPR030491. TBP_CS.
IPR033710. TBP_eukaryotic.
[Graphical view]
PANTHERiPTHR10126. PTHR10126. 1 hit.
PfamiPF00352. TBP. 2 hits.
[Graphical view]
PRINTSiPR00686. TIFACTORIID.
PROSITEiPS00351. TFIID. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTBP_DANRE
AccessioniPrimary (citable) accession number: Q7SXL3
Secondary accession number(s): Q71H62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: October 1, 2003
Last modified: November 30, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.