Reviewed, UniProtKB/Swiss-Prot Q7SXF1 (DHCR7_DANRE)

Names and origin

Protein names

Recommended name:
    7-dehydrocholesterol reductase
      Short name=7-DHC reductase
    EC=1.3.1.21
Alternative name(s):
    Sterol Delta(7)-reductase

Gene names

Name:

dhcr7

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC) By similarity.
Catalytic activity	Cholesterol + NADP(+) = cholesta-5,7-dien-3-beta-ol + NADPH.
Pathway	Steroid biosynthesis; cholesterol biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane proteinBy similarity.
Sequence similarities	Belongs to the ERG4/ERG24 family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	7-dehydrocholesterol reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	7-dehydrocholesterol reductase		PRO_0000207505
Regions
	Transmembrane	43 – 63	21	Potential
	Transmembrane	97 – 117	21	Potential
	Transmembrane	180 – 200	21	Potential
	Transmembrane	269 – 289	21	Potential
	Transmembrane	309 – 329	21	Potential
	Transmembrane	333 – 353	21	Potential
	Transmembrane	424 – 444	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7SXF1-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: EFFDB76773E4ECD7	FASTA	478	55,189
	10 20 30 40 50 60 MMASDRVRKR HKGSANGAQT VEKEPSKEPA QWGRAWEVDW FSLSGVILLL CFAPFLVSFF 70 80 90 100 110 120 IMACDQYQCS ISHPLLDLYN GDATLFTIWN RAPSFTWAAA KIYAIWVTFQ VVLYMCVPDF 130 140 150 160 170 180 LHKILPGYVG GVQDGARTPA GLINKYEVNG LQCWLITHVL WVLNAQHFHW FSPTIIIDNW 190 200 210 220 230 240 IPLLWCTNIL GYAVSTFAFI KAYLFPTNPE DCKFTGNMFY NYMMGIEFNP RIGKWFDFKL 250 260 270 280 290 300 FFNGRPGIVA WTLINLSYAA KQQELYGYVT NSMILVNVLQ AVYVVDFFWN EAWYLKTIDI 310 320 330 340 350 360 CHDHFGWYLG WGDCVWLPFL YTLQGLYLVY NPIQLSTPHA AGVLILGLVG YYIFRVTNHQ 370 380 390 400 410 420 KDLFRRTEGN CSIWGKKPTF IECSYQSADG AIHKSKLMTS GFWGVARHMN YTGDLMGSLA 430 440 450 460 470 YCLACGGNHL LPYFYIIYMT ILLVHRCIRD EHRCSNKYGK DWERYTAAVS YRLLPNIF

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC055631 mRNA. Translation: AAH55631.1.
RefSeq	NP_958487.1.
UniGene	Dr.83800
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSDARG00000015564. Danio rerio. [Contig view]
GeneID	378446.
KEGG	dre:378446.
Organism-specific databases
ZFIN	ZDB-GENE-030912-9. dhcr7.
Phylogenomic databases
HOGENOM	Q7SXF1.
HOVERGEN	Q7SXF1.
Gene expression databases
ArrayExpress	Q7SXF1.
Family and domain databases
InterPro	IPR001171. ERG4_ERG24. [Graphical view]
Pfam	PF01222. ERG4_ERG24. 1 hit. [Graphical view]
PROSITE	PS01017. STEROL_REDUCT_1. 1 hit. PS01018. STEROL_REDUCT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHCR7_DANRE

Accession

Primary (citable) accession number: Q7SXF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	October 1, 2003
Last modified:	November 4, 2008
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project