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Q7SXA9

- MUS81_DANRE

UniProt

Q7SXA9 - MUS81_DANRE

Protein

Crossover junction endonuclease MUS81

Gene

mus81

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Interacts with eme1 and eme2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks By similarity.By similarity

    Cofactori

    Magnesium.By similarity

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. endonuclease activity Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-KW
    2. DNA repair Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Crossover junction endonuclease MUS81 (EC:3.1.22.-)
    Gene namesi
    Name:mus81
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Unplaced

    Organism-specific databases

    ZFINiZDB-GENE-040426-2923. mus81.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 604604Crossover junction endonuclease MUS81PRO_0000223637Add
    BLAST

    Proteomic databases

    PRIDEiQ7SXA9.

    Interactioni

    Subunit structurei

    Binds eme1.By similarity

    Structurei

    Secondary structure

    1
    604
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi310 – 3178
    Helixi319 – 3213
    Helixi332 – 3387
    Turni339 – 3413
    Beta strandi344 – 3463
    Beta strandi353 – 3608
    Beta strandi376 – 38712
    Helixi388 – 3969
    Helixi400 – 4089
    Beta strandi413 – 4219
    Beta strandi423 – 4253
    Helixi426 – 4294
    Helixi433 – 44513
    Beta strandi450 – 4534
    Helixi457 – 47317
    Turni474 – 4774
    Beta strandi480 – 4823
    Beta strandi504 – 5085
    Helixi509 – 51911
    Helixi524 – 5329
    Helixi540 – 54910
    Helixi553 – 56210
    Helixi566 – 5694
    Turni570 – 5756
    Turni579 – 5824
    Helixi586 – 59813

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2ZIUX-ray2.70A295-604[»]
    2ZIVX-ray2.70A295-604[»]
    2ZIWX-ray2.80A295-604[»]
    ProteinModelPortaliQ7SXA9.
    SMRiQ7SXA9. Positions 303-604.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ7SXA9.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini314 – 423110ERCC4Add
    BLAST

    Sequence similaritiesi

    Belongs to the XPF family.Curated
    Contains 1 ERCC4 domain.Curated

    Phylogenomic databases

    eggNOGiCOG1948.
    HOGENOMiHOG000113699.
    HOVERGENiHBG052538.
    KOiK08991.

    Family and domain databases

    Gene3Di1.10.8.310. 1 hit.
    3.40.50.10130. 1 hit.
    InterProiIPR010996. DNA_pol_b-like_N.
    IPR020819. DNA_repair_nuc_XPF/helicase.
    IPR006166. ERCC4_domain.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR027420. PolB_N.
    IPR011335. Restrct_endonuc-II-like.
    [Graphical view]
    PfamiPF02732. ERCC4. 1 hit.
    [Graphical view]
    SMARTiSM00891. ERCC4. 1 hit.
    SM00278. HhH1. 1 hit.
    [Graphical view]
    SUPFAMiSSF47802. SSF47802. 1 hit.
    SSF52980. SSF52980. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q7SXA9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPTDQVCLGR KRPLPSCPNP LFLQWLTELR DSAKEKGLKT HFVYQKAINS    50
    LKKYPLPLKN GKEAKILQNF GDGICKILDE RLQKHYRENG SDAAVHLASS 100
    KQMEESQKEP SGNFSEHTKL TQKEVRKEKG AKKKREYVPQ KRSGGYAVLL 150
    TLYRHMQMPG SKGFMFRNEL QTEAQPLCEK SFTVPDLGSK YTAWSSVSTL 200
    IQKELVVKTH NPARYSLTDQ GLSLAEKLDS EETGTRHEDV DSQDGQNVVD 250
    LTLEEEDEDE EKESWSSERP AVALPVNQAR GLMSESDPMG KSQTSETGRT 300
    AMGWHLSPGS YDIVLCVDLC ETTGGSSVRK QELVKELQRN SVTFDVRKLN 350
    VGDFLWVARE RVTPVPGQLR PPVGKELVLD YIIERKRMDD LCGSIIDGRF 400
    REQKFRLKRC GLRKPIYLVE ECGSAAAHLS IPESTLQQAI VNTQVVDGFF 450
    VKRVQDAKES AAYLTIMTRY LQKLYQNCTL LCRSRELEGD GEAESEKMVA 500
    NLSCSLMAFT EFNYGAIKNK CQTVREVFAR QLMQISGVSG DKAAAVLKHY 550
    STVSSLLQAY DKCSSETEKE KLLSSVKYGK LKRNLGPALS RTIYQLYCTR 600
    GPLS 604
    Length:604
    Mass (Da):68,017
    Last modified:October 1, 2003 - v1
    Checksum:i5AC33516404D3CE9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC055679 mRNA. Translation: AAH55679.1.
    RefSeqiNP_998683.1. NM_213518.1.
    UniGeneiDr.977.

    Genome annotation databases

    GeneIDi406839.
    KEGGidre:406839.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC055679 mRNA. Translation: AAH55679.1 .
    RefSeqi NP_998683.1. NM_213518.1.
    UniGenei Dr.977.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2ZIU X-ray 2.70 A 295-604 [» ]
    2ZIV X-ray 2.70 A 295-604 [» ]
    2ZIW X-ray 2.80 A 295-604 [» ]
    ProteinModelPortali Q7SXA9.
    SMRi Q7SXA9. Positions 303-604.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q7SXA9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 406839.
    KEGGi dre:406839.

    Organism-specific databases

    CTDi 80198.
    ZFINi ZDB-GENE-040426-2923. mus81.

    Phylogenomic databases

    eggNOGi COG1948.
    HOGENOMi HOG000113699.
    HOVERGENi HBG052538.
    KOi K08991.

    Miscellaneous databases

    EvolutionaryTracei Q7SXA9.
    NextBioi 20818343.
    PROi Q7SXA9.

    Family and domain databases

    Gene3Di 1.10.8.310. 1 hit.
    3.40.50.10130. 1 hit.
    InterProi IPR010996. DNA_pol_b-like_N.
    IPR020819. DNA_repair_nuc_XPF/helicase.
    IPR006166. ERCC4_domain.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR027420. PolB_N.
    IPR011335. Restrct_endonuc-II-like.
    [Graphical view ]
    Pfami PF02732. ERCC4. 1 hit.
    [Graphical view ]
    SMARTi SM00891. ERCC4. 1 hit.
    SM00278. HhH1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47802. SSF47802. 1 hit.
    SSF52980. SSF52980. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: AB.
    2. "Crystal structure of the Mus81-Eme1 complex."
      Chang J.H., Kim J.J., Choi J.M., Lee J.H., Cho Y.
      Genes Dev. 22:1093-1106(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 295-604.

    Entry informationi

    Entry nameiMUS81_DANRE
    AccessioniPrimary (citable) accession number: Q7SXA9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2006
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3