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Q7SXA9 (MUS81_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endonuclease MUS81

EC=3.1.22.-
Gene names
Name:mus81
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length604 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Interacts with eme1 and eme2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks By similarity.

Cofactor

Magnesium By similarity.

Subunit structure

Binds eme1 By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the XPF family.

Contains 1 ERCC4 domain.

Ontologies

Keywords
   Biological processDNA damage
DNA recombination
DNA repair
   Cellular componentNucleus
   LigandMagnesium
Metal-binding
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA recombination

Inferred from electronic annotation. Source: UniProtKB-KW

DNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 604604Crossover junction endonuclease MUS81
PRO_0000223637

Regions

Domain314 – 423110ERCC4

Secondary structure

............................................... 604
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q7SXA9 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 5AC33516404D3CE9

FASTA60468,017
        10         20         30         40         50         60 
MPTDQVCLGR KRPLPSCPNP LFLQWLTELR DSAKEKGLKT HFVYQKAINS LKKYPLPLKN 

        70         80         90        100        110        120 
GKEAKILQNF GDGICKILDE RLQKHYRENG SDAAVHLASS KQMEESQKEP SGNFSEHTKL 

       130        140        150        160        170        180 
TQKEVRKEKG AKKKREYVPQ KRSGGYAVLL TLYRHMQMPG SKGFMFRNEL QTEAQPLCEK 

       190        200        210        220        230        240 
SFTVPDLGSK YTAWSSVSTL IQKELVVKTH NPARYSLTDQ GLSLAEKLDS EETGTRHEDV 

       250        260        270        280        290        300 
DSQDGQNVVD LTLEEEDEDE EKESWSSERP AVALPVNQAR GLMSESDPMG KSQTSETGRT 

       310        320        330        340        350        360 
AMGWHLSPGS YDIVLCVDLC ETTGGSSVRK QELVKELQRN SVTFDVRKLN VGDFLWVARE 

       370        380        390        400        410        420 
RVTPVPGQLR PPVGKELVLD YIIERKRMDD LCGSIIDGRF REQKFRLKRC GLRKPIYLVE 

       430        440        450        460        470        480 
ECGSAAAHLS IPESTLQQAI VNTQVVDGFF VKRVQDAKES AAYLTIMTRY LQKLYQNCTL 

       490        500        510        520        530        540 
LCRSRELEGD GEAESEKMVA NLSCSLMAFT EFNYGAIKNK CQTVREVFAR QLMQISGVSG 

       550        560        570        580        590        600 
DKAAAVLKHY STVSSLLQAY DKCSSETEKE KLLSSVKYGK LKRNLGPALS RTIYQLYCTR 


GPLS 

« Hide

References

« Hide 'large scale' references
[1]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: AB.
[2]"Crystal structure of the Mus81-Eme1 complex."
Chang J.H., Kim J.J., Choi J.M., Lee J.H., Cho Y.
Genes Dev. 22:1093-1106(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 295-604.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC055679 mRNA. Translation: AAH55679.1.
RefSeqNP_998683.1. NM_213518.1.
UniGeneDr.977.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZIUX-ray2.70A295-604[»]
2ZIVX-ray2.70A295-604[»]
2ZIWX-ray2.80A295-604[»]
ProteinModelPortalQ7SXA9.
SMRQ7SXA9. Positions 303-604.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ7SXA9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID406839.
KEGGdre:406839.

Organism-specific databases

CTD80198.
ZFINZDB-GENE-040426-2923. mus81.

Phylogenomic databases

eggNOGCOG1948.
HOGENOMHOG000113699.
HOVERGENHBG052538.
KOK08991.

Family and domain databases

Gene3D1.10.8.310. 1 hit.
3.40.50.10130. 1 hit.
InterProIPR010996. DNA_pol_b-like_N.
IPR020819. DNA_repair_nuc_XPF/helicase.
IPR006166. ERCC4_domain.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027420. PolB_N.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PfamPF02732. ERCC4. 1 hit.
[Graphical view]
SMARTSM00891. ERCC4. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMSSF47802. SSF47802. 1 hit.
SSF52980. SSF52980. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ7SXA9.
NextBio20818343.
PROQ7SXA9.

Entry information

Entry nameMUS81_DANRE
AccessionPrimary (citable) accession number: Q7SXA9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: October 1, 2003
Last modified: April 16, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references