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Q7SXA9

- MUS81_DANRE

UniProt

Q7SXA9 - MUS81_DANRE

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Protein

Crossover junction endonuclease MUS81

Gene

mus81

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Interacts with eme1 and eme2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks (By similarity).By similarity

Cofactori

Magnesium.By similarity

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. endonuclease activity Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA recombination Source: UniProtKB-KW
  2. DNA repair Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endonuclease MUS81 (EC:3.1.22.-)
Gene namesi
Name:mus81
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Unplaced

Organism-specific databases

ZFINiZDB-GENE-040426-2923. mus81.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 604604Crossover junction endonuclease MUS81PRO_0000223637Add
BLAST

Proteomic databases

PRIDEiQ7SXA9.

Interactioni

Subunit structurei

Binds eme1.By similarity

Structurei

Secondary structure

1
604
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi310 – 3178
Helixi319 – 3213
Helixi332 – 3387
Turni339 – 3413
Beta strandi344 – 3463
Beta strandi353 – 3608
Beta strandi376 – 38712
Helixi388 – 3969
Helixi400 – 4089
Beta strandi413 – 4219
Beta strandi423 – 4253
Helixi426 – 4294
Helixi433 – 44513
Beta strandi450 – 4534
Helixi457 – 47317
Turni474 – 4774
Beta strandi480 – 4823
Beta strandi504 – 5085
Helixi509 – 51911
Helixi524 – 5329
Helixi540 – 54910
Helixi553 – 56210
Helixi566 – 5694
Turni570 – 5756
Turni579 – 5824
Helixi586 – 59813

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZIUX-ray2.70A295-604[»]
2ZIVX-ray2.70A295-604[»]
2ZIWX-ray2.80A295-604[»]
ProteinModelPortaliQ7SXA9.
SMRiQ7SXA9. Positions 303-604.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7SXA9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini314 – 423110ERCC4Add
BLAST

Sequence similaritiesi

Belongs to the XPF family.Curated
Contains 1 ERCC4 domain.Curated

Phylogenomic databases

eggNOGiCOG1948.
HOGENOMiHOG000113699.
HOVERGENiHBG052538.
InParanoidiQ7SXA9.
KOiK08991.

Family and domain databases

Gene3Di1.10.8.310. 1 hit.
3.40.50.10130. 1 hit.
InterProiIPR010996. DNA_pol_b-like_N.
IPR020819. DNA_repair_nuc_XPF/helicase.
IPR006166. ERCC4_domain.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027420. PolB_N.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PfamiPF02732. ERCC4. 1 hit.
[Graphical view]
SMARTiSM00891. ERCC4. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7SXA9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPTDQVCLGR KRPLPSCPNP LFLQWLTELR DSAKEKGLKT HFVYQKAINS
60 70 80 90 100
LKKYPLPLKN GKEAKILQNF GDGICKILDE RLQKHYRENG SDAAVHLASS
110 120 130 140 150
KQMEESQKEP SGNFSEHTKL TQKEVRKEKG AKKKREYVPQ KRSGGYAVLL
160 170 180 190 200
TLYRHMQMPG SKGFMFRNEL QTEAQPLCEK SFTVPDLGSK YTAWSSVSTL
210 220 230 240 250
IQKELVVKTH NPARYSLTDQ GLSLAEKLDS EETGTRHEDV DSQDGQNVVD
260 270 280 290 300
LTLEEEDEDE EKESWSSERP AVALPVNQAR GLMSESDPMG KSQTSETGRT
310 320 330 340 350
AMGWHLSPGS YDIVLCVDLC ETTGGSSVRK QELVKELQRN SVTFDVRKLN
360 370 380 390 400
VGDFLWVARE RVTPVPGQLR PPVGKELVLD YIIERKRMDD LCGSIIDGRF
410 420 430 440 450
REQKFRLKRC GLRKPIYLVE ECGSAAAHLS IPESTLQQAI VNTQVVDGFF
460 470 480 490 500
VKRVQDAKES AAYLTIMTRY LQKLYQNCTL LCRSRELEGD GEAESEKMVA
510 520 530 540 550
NLSCSLMAFT EFNYGAIKNK CQTVREVFAR QLMQISGVSG DKAAAVLKHY
560 570 580 590 600
STVSSLLQAY DKCSSETEKE KLLSSVKYGK LKRNLGPALS RTIYQLYCTR

GPLS
Length:604
Mass (Da):68,017
Last modified:October 1, 2003 - v1
Checksum:i5AC33516404D3CE9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC055679 mRNA. Translation: AAH55679.1.
RefSeqiNP_998683.1. NM_213518.1.
UniGeneiDr.977.

Genome annotation databases

GeneIDi406839.
KEGGidre:406839.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC055679 mRNA. Translation: AAH55679.1 .
RefSeqi NP_998683.1. NM_213518.1.
UniGenei Dr.977.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2ZIU X-ray 2.70 A 295-604 [» ]
2ZIV X-ray 2.70 A 295-604 [» ]
2ZIW X-ray 2.80 A 295-604 [» ]
ProteinModelPortali Q7SXA9.
SMRi Q7SXA9. Positions 303-604.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q7SXA9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 406839.
KEGGi dre:406839.

Organism-specific databases

CTDi 80198.
ZFINi ZDB-GENE-040426-2923. mus81.

Phylogenomic databases

eggNOGi COG1948.
HOGENOMi HOG000113699.
HOVERGENi HBG052538.
InParanoidi Q7SXA9.
KOi K08991.

Miscellaneous databases

EvolutionaryTracei Q7SXA9.
NextBioi 20818343.
PROi Q7SXA9.

Family and domain databases

Gene3Di 1.10.8.310. 1 hit.
3.40.50.10130. 1 hit.
InterProi IPR010996. DNA_pol_b-like_N.
IPR020819. DNA_repair_nuc_XPF/helicase.
IPR006166. ERCC4_domain.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027420. PolB_N.
IPR011335. Restrct_endonuc-II-like.
[Graphical view ]
Pfami PF02732. ERCC4. 1 hit.
[Graphical view ]
SMARTi SM00891. ERCC4. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view ]
SUPFAMi SSF47802. SSF47802. 1 hit.
SSF52980. SSF52980. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: AB.
  2. "Crystal structure of the Mus81-Eme1 complex."
    Chang J.H., Kim J.J., Choi J.M., Lee J.H., Cho Y.
    Genes Dev. 22:1093-1106(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 295-604.

Entry informationi

Entry nameiMUS81_DANRE
AccessioniPrimary (citable) accession number: Q7SXA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: October 1, 2003
Last modified: October 29, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3