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Protein

Crossover junction endonuclease MUS81

Gene

mus81

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Interacts with eme1 and eme2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks (By similarity).By similarity

Cofactori

Mg2+By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endonuclease MUS81 (EC:3.1.22.-)
Gene namesi
Name:mus81
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-040426-2923. mus81.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002236371 – 604Crossover junction endonuclease MUS81Add BLAST604

Proteomic databases

PaxDbiQ7SXA9.
PRIDEiQ7SXA9.

Interactioni

Subunit structurei

Binds eme1.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000091555.

Structurei

Secondary structure

1604
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi310 – 317Combined sources8
Helixi319 – 321Combined sources3
Helixi332 – 338Combined sources7
Turni339 – 341Combined sources3
Beta strandi344 – 346Combined sources3
Beta strandi353 – 360Combined sources8
Beta strandi376 – 387Combined sources12
Helixi388 – 396Combined sources9
Helixi400 – 408Combined sources9
Beta strandi413 – 421Combined sources9
Beta strandi423 – 425Combined sources3
Helixi426 – 429Combined sources4
Helixi433 – 445Combined sources13
Beta strandi450 – 453Combined sources4
Helixi457 – 473Combined sources17
Turni474 – 477Combined sources4
Beta strandi480 – 482Combined sources3
Beta strandi504 – 508Combined sources5
Helixi509 – 519Combined sources11
Helixi524 – 532Combined sources9
Helixi540 – 549Combined sources10
Helixi553 – 562Combined sources10
Helixi566 – 569Combined sources4
Turni570 – 575Combined sources6
Turni579 – 582Combined sources4
Helixi586 – 598Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZIUX-ray2.70A295-604[»]
2ZIVX-ray2.70A295-604[»]
2ZIWX-ray2.80A295-604[»]
ProteinModelPortaliQ7SXA9.
SMRiQ7SXA9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7SXA9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini314 – 423ERCC4Add BLAST110

Sequence similaritiesi

Belongs to the XPF family.Curated
Contains 1 ERCC4 domain.Curated

Phylogenomic databases

eggNOGiKOG2379. Eukaryota.
COG1948. LUCA.
HOGENOMiHOG000113699.
HOVERGENiHBG052538.
InParanoidiQ7SXA9.
KOiK08991.

Family and domain databases

Gene3Di1.10.8.310. 1 hit.
3.40.50.10130. 1 hit.
InterProiIPR010996. DNA_pol_b-like_N.
IPR020819. DNA_repair_nuc_XPF/helicase.
IPR006166. ERCC4_domain.
IPR033309. Mus81.
IPR027420. PolB_N.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PANTHERiPTHR13451:SF0. PTHR13451:SF0. 1 hit.
PfamiPF02732. ERCC4. 1 hit.
[Graphical view]
SMARTiSM00891. ERCC4. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7SXA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTDQVCLGR KRPLPSCPNP LFLQWLTELR DSAKEKGLKT HFVYQKAINS
60 70 80 90 100
LKKYPLPLKN GKEAKILQNF GDGICKILDE RLQKHYRENG SDAAVHLASS
110 120 130 140 150
KQMEESQKEP SGNFSEHTKL TQKEVRKEKG AKKKREYVPQ KRSGGYAVLL
160 170 180 190 200
TLYRHMQMPG SKGFMFRNEL QTEAQPLCEK SFTVPDLGSK YTAWSSVSTL
210 220 230 240 250
IQKELVVKTH NPARYSLTDQ GLSLAEKLDS EETGTRHEDV DSQDGQNVVD
260 270 280 290 300
LTLEEEDEDE EKESWSSERP AVALPVNQAR GLMSESDPMG KSQTSETGRT
310 320 330 340 350
AMGWHLSPGS YDIVLCVDLC ETTGGSSVRK QELVKELQRN SVTFDVRKLN
360 370 380 390 400
VGDFLWVARE RVTPVPGQLR PPVGKELVLD YIIERKRMDD LCGSIIDGRF
410 420 430 440 450
REQKFRLKRC GLRKPIYLVE ECGSAAAHLS IPESTLQQAI VNTQVVDGFF
460 470 480 490 500
VKRVQDAKES AAYLTIMTRY LQKLYQNCTL LCRSRELEGD GEAESEKMVA
510 520 530 540 550
NLSCSLMAFT EFNYGAIKNK CQTVREVFAR QLMQISGVSG DKAAAVLKHY
560 570 580 590 600
STVSSLLQAY DKCSSETEKE KLLSSVKYGK LKRNLGPALS RTIYQLYCTR

GPLS
Length:604
Mass (Da):68,017
Last modified:October 1, 2003 - v1
Checksum:i5AC33516404D3CE9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC055679 mRNA. Translation: AAH55679.1.
RefSeqiNP_998683.1. NM_213518.1.
UniGeneiDr.977.

Genome annotation databases

GeneIDi406839.
KEGGidre:406839.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC055679 mRNA. Translation: AAH55679.1.
RefSeqiNP_998683.1. NM_213518.1.
UniGeneiDr.977.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZIUX-ray2.70A295-604[»]
2ZIVX-ray2.70A295-604[»]
2ZIWX-ray2.80A295-604[»]
ProteinModelPortaliQ7SXA9.
SMRiQ7SXA9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000091555.

Proteomic databases

PaxDbiQ7SXA9.
PRIDEiQ7SXA9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi406839.
KEGGidre:406839.

Organism-specific databases

CTDi80198.
ZFINiZDB-GENE-040426-2923. mus81.

Phylogenomic databases

eggNOGiKOG2379. Eukaryota.
COG1948. LUCA.
HOGENOMiHOG000113699.
HOVERGENiHBG052538.
InParanoidiQ7SXA9.
KOiK08991.

Miscellaneous databases

EvolutionaryTraceiQ7SXA9.
PROiQ7SXA9.

Family and domain databases

Gene3Di1.10.8.310. 1 hit.
3.40.50.10130. 1 hit.
InterProiIPR010996. DNA_pol_b-like_N.
IPR020819. DNA_repair_nuc_XPF/helicase.
IPR006166. ERCC4_domain.
IPR033309. Mus81.
IPR027420. PolB_N.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PANTHERiPTHR13451:SF0. PTHR13451:SF0. 1 hit.
PfamiPF02732. ERCC4. 1 hit.
[Graphical view]
SMARTiSM00891. ERCC4. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52980. SSF52980. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMUS81_DANRE
AccessioniPrimary (citable) accession number: Q7SXA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: October 1, 2003
Last modified: November 30, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.