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Q7SX99 (FUMH_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fumarate hydratase, mitochondrial

Short name=Fumarase
EC=4.2.1.2
Gene names
Name:fh
ORF Names:zgc:66253
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length509 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

(S)-malate = fumarate + H2O. HAMAP-Rule MF_00743

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. HAMAP-Rule MF_00743

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00743

Subcellular location

Mitochondrion By similarity HAMAP-Rule MF_00743.

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.

Sequence similarities

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Cellular componentMitochondrion
   DomainTransit peptide
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processfumarate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentmitochondrion

Inferred from electronic annotation. Source: UniProtKB-SubCell

tricarboxylic acid cycle enzyme complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionfumarate hydratase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4343Mitochondrion By similarity
Chain44 – 509466Fumarate hydratase, mitochondrial HAMAP-Rule MF_00743
PRO_0000010327

Regions

Region175 – 1784B site By similarity
Region185 – 1873Substrate binding By similarity

Sites

Binding site1461Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7SX99 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 36A829D58EE18678

FASTA50954,861
        10         20         30         40         50         60 
MYRSARSLHR FSASLSDLRA AQRSIKARNV CPAPGLRHQT VRMASSEAFR IERDTFGELK 

        70         80         90        100        110        120 
VPSDKYYGAQ TVRSTMNFRI GGVTERMPIQ VIRAFGILKK AAAEVNKDYG LDPKIADAIM 

       130        140        150        160        170        180 
KAADEVESGK LDDHFPLVVW QTGSGTQTNM NVNEVISNRA IEMLGGKLGS KDPVHPNDHV 

       190        200        210        220        230        240 
NKSQSSNDTF PTAMHIAAAK EVHEVLLPGL QTLHDALAAK AEQFKDIIKI GRTHTQDAVP 

       250        260        270        280        290        300 
LSLGQEFGGY VQQVKYSIAR VKASLPRVYE LAAGGTAVGT GLNTRIGFAE KVADKVSALT 

       310        320        330        340        350        360 
GLPFVTAANK FEALAAHDAL VELSGALNTV AVSMMKIAND IRFLGSGPRS GLGELILPEN 

       370        380        390        400        410        420 
EPGSSIMPGK VNPTQCEAMT MVAAQVMGNH VAVTVGGSNG HFELNVFKPM IIKNVLNSAR 

       430        440        450        460        470        480 
LLGDASVSFT NNCVVGIEAN TERINKLMSE SLMLVTALNP HIGYDKAAKI AKTAHKDGST 

       490        500 
LKEAALKLGF LNEQQFEEWV RPHDMLGPK 

« Hide

References

[1]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: AB.
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC055566 mRNA. Translation: AAH55566.1.
BC066484 mRNA. Translation: AAH66484.1.
RefSeqNP_957257.1. NM_200963.1.
UniGeneDr.104452.

3D structure databases

ProteinModelPortalQ7SX99.
SMRQ7SX99. Positions 48-509.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7955.ENSDARP00000097494.

Proteomic databases

PRIDEQ7SX99.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID393938.
KEGGdre:393938.

Organism-specific databases

CTD2271.
ZFINZDB-GENE-010724-6. fh.

Phylogenomic databases

eggNOGCOG0114.
HOGENOMHOG000061736.
HOVERGENHBG002183.
KOK01679.
PhylomeDBQ7SX99.

Enzyme and pathway databases

UniPathwayUPA00223; UER01007.

Family and domain databases

Gene3D1.10.275.10. 1 hit.
HAMAPMF_00743. FumaraseC.
InterProIPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view]
PANTHERPTHR11444. PTHR11444. 1 hit.
PfamPF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSPR00149. FUMRATELYASE.
SUPFAMSSF48557. SSF48557. 1 hit.
TIGRFAMsTIGR00979. fumC_II. 1 hit.
PROSITEPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20814910.
PROQ7SX99.

Entry information

Entry nameFUMH_DANRE
AccessionPrimary (citable) accession number: Q7SX99
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: October 1, 2003
Last modified: April 16, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways