Q7SX99 (FUMH_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fumarate hydratase, mitochondrial
Short name=Fumarase
EC=4.2.1.2

Gene names

Name:	fh
ORF Names:	zgc:66253

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Mitochondrion By similarity.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular_function	fumarate hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 43	43	Mitochondrion By similarity
	Chain	44 – 509	466	Fumarate hydratase, mitochondrial		PRO_0000010327
Regions
	Region	175 – 178	4	B site By similarity
	Region	185 – 187	3	Substrate binding By similarity
Sites
	Binding site	146	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7SX99 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 36A829D58EE18678	FASTA	509	54,861
	10 20 30 40 50 60 MYRSARSLHR FSASLSDLRA AQRSIKARNV CPAPGLRHQT VRMASSEAFR IERDTFGELK 70 80 90 100 110 120 VPSDKYYGAQ TVRSTMNFRI GGVTERMPIQ VIRAFGILKK AAAEVNKDYG LDPKIADAIM 130 140 150 160 170 180 KAADEVESGK LDDHFPLVVW QTGSGTQTNM NVNEVISNRA IEMLGGKLGS KDPVHPNDHV 190 200 210 220 230 240 NKSQSSNDTF PTAMHIAAAK EVHEVLLPGL QTLHDALAAK AEQFKDIIKI GRTHTQDAVP 250 260 270 280 290 300 LSLGQEFGGY VQQVKYSIAR VKASLPRVYE LAAGGTAVGT GLNTRIGFAE KVADKVSALT 310 320 330 340 350 360 GLPFVTAANK FEALAAHDAL VELSGALNTV AVSMMKIAND IRFLGSGPRS GLGELILPEN 370 380 390 400 410 420 EPGSSIMPGK VNPTQCEAMT MVAAQVMGNH VAVTVGGSNG HFELNVFKPM IIKNVLNSAR 430 440 450 460 470 480 LLGDASVSFT NNCVVGIEAN TERINKLMSE SLMLVTALNP HIGYDKAAKI AKTAHKDGST 490 500 LKEAALKLGF LNEQQFEEWV RPHDMLGPK

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: AB. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC055566 mRNA. Translation: AAH55566.1. BC066484 mRNA. Translation: AAH66484.1.
IPI	IPI00488038.
RefSeq	NP_957257.1. NM_200963.1.
UniGene	Dr.104452.
3D structure databases
ProteinModelPortal	Q7SX99.
SMR	Q7SX99. Positions 48-509.
ModBase	Search...
Protein-protein interaction databases
STRING	7955.ENSDARP00000097494.
Proteomic databases
PRIDE	Q7SX99.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	393938.
KEGG	dre:393938.
Organism-specific databases
CTD	2271.
ZFIN	ZDB-GENE-010724-6. fh.
Phylogenomic databases
eggNOG	COG0114.
HOGENOM	HOG000061736.
HOVERGEN	HBG002183.
KO	K01679.
OrthoDB	EOG4F1X31.
Enzyme and pathway databases
UniPathway	UPA00223; UER01007.
Family and domain databases
Gene3D	1.10.275.10. 1 hit.
InterPro	IPR003031. D_crystallin. IPR005677. Fum_hydII. IPR024083. Fumarase/histidase_N. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR022761. Fumarate_lyase_N. IPR008948. L-Aspartase-like. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
TIGRFAMs	TIGR00979. fumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20814910.

Entry information

Entry name

FUMH_DANRE

Accession

Primary (citable) accession number: Q7SX99

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2003
Last modified:	April 3, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program