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Q7SIG7

- DPOL_DESST

UniProt

Q7SIG7 - DPOL_DESST

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Protein

DNA polymerase

Gene

pol

Organism
Desulfurococcus sp. (strain Tok)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Thermostable DNA polymerase. In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity.1 Publication

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).1 Publication

Cofactori

Binds 2 magnesium ions.1 Publication

Enzyme regulationi

DNA polymerase activity strongly inhibited by uracil-containing oligonucleotides.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi141 – 1411Magnesium 11 Publication
Metal bindingi141 – 1411Magnesium 21 Publication
Metal bindingi143 – 1431Magnesium 21 Publication
Metal bindingi315 – 3151Magnesium 11 Publication
Metal bindingi315 – 3151Magnesium 21 Publication

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
  3. exonuclease activity Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW
  5. nucleotide binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase (EC:2.7.7.7)
Alternative name(s):
D Tok Pol
Gene namesi
Name:pol
OrganismiDesulfurococcus sp. (strain Tok)
Taxonomic identifieri108142 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeDesulfurococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 773773DNA polymerasePRO_0000278146Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi428 ↔ 4421 Publication
Disulfide bondi506 ↔ 5091 Publication

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1
773
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 109
Beta strandi13 – 2210
Beta strandi25 – 317
Beta strandi37 – 448
Helixi45 – 473
Turni49 – 535
Beta strandi55 – 584
Beta strandi61 – 644
Beta strandi67 – 8620
Helixi92 – 1009
Beta strandi106 – 1116
Helixi116 – 1238
Beta strandi137 – 1437
Beta strandi159 – 1646
Beta strandi167 – 1748
Beta strandi181 – 1833
Helixi187 – 20115
Beta strandi204 – 2107
Turni211 – 2144
Helixi215 – 22410
Turni225 – 2273
Beta strandi240 – 25112
Beta strandi255 – 2595
Helixi260 – 2678
Helixi275 – 2828
Helixi294 – 3007
Helixi302 – 3054
Helixi308 – 33730
Helixi341 – 3444
Helixi349 – 36315
Helixi374 – 3807
Beta strandi397 – 40711
Helixi408 – 4158
Helixi420 – 4223
Beta strandi429 – 4335
Turni435 – 4373
Beta strandi440 – 4423
Helixi448 – 46922
Helixi473 – 49018
Helixi493 – 4975
Helixi507 – 53226
Beta strandi535 – 5395
Beta strandi541 – 5477
Helixi553 – 56715
Helixi568 – 5703
Beta strandi576 – 59015
Beta strandi593 – 5975
Beta strandi603 – 6075
Helixi617 – 63115
Helixi636 – 65015
Turni651 – 6533
Helixi657 – 6593
Beta strandi662 – 6643
Helixi678 – 6825
Turni687 – 6904
Beta strandi699 – 7024
Beta strandi709 – 7113
Beta strandi717 – 7193
Turni722 – 7243
Turni729 – 7324
Helixi733 – 7375
Helixi738 – 7469
Turni747 – 7493
Helixi752 – 7543

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D5AX-ray2.40A1-756[»]
1QQCX-ray2.60A1-773[»]
ProteinModelPortaliQ7SIG7.
SMRiQ7SIG7. Positions 1-756.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7SIG7.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 131131N-terminal domain1 PublicationAdd
BLAST
Regioni133 – 385253Exonuclease domain1 PublicationAdd
BLAST
Regioni390 – 773384Polymerase domain1 PublicationAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Sequence Analysis

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.90.1600.10. 2 hits.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 2 hits.
[Graphical view]
PRINTSiPR00106. DNAPOLB.
SMARTiSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7SIG7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MILDADYITE DGKPVIRVFK KEKGEFKIDY DRDFEPYIYA LLKDDSAIED
60 70 80 90 100
IKKITAERHG TTVRVTRAER VKKKFLGRPV EVWKLYFTHP QDVPAIRDKI
110 120 130 140 150
REHPAVVDIY EYDIPFAKRY LIDRGLIPME GDEELRMLAF DIETLYHEGE
160 170 180 190 200
EFGEGPILMI SYADEEGARV ITWKNIDLPY VESVSTEKEM IKRFLKVIQE
210 220 230 240 250
KDPDVLITYN GDNFDFAYLK KRSEMLGVKF ILGRDGSEPK IQRMGDRFAV
260 270 280 290 300
EVKGRIHFDL YPVIRRTINL PTYTLETVYE PVFGQPKEKV YAEEIARAWE
310 320 330 340 350
SGEGLERVAR YSMEDAKATY ELGKEFFPME AQLSRLVGQS LWDVSRSSTG
360 370 380 390 400
NLVEWFLLRK AYERNDVAPN KPDERELARR TESYAGGYVK EPEKGLWENI
410 420 430 440 450
VYLDYKSLYP SIIITHNVSP DTLNREGCRE YDVAPQVGHR FCKDFPGFIP
460 470 480 490 500
SLLGDLLEER QKVKKKMKAT VDPIERKLLD YRQRAIKILA NSYYGYYAYA
510 520 530 540 550
NARWYCRECA ESVTAWGRQY IETTMREIEE KFGFKVLYAD TDGFFATIPG
560 570 580 590 600
ADAETVKNKA KEFLNYINPR LPGLLELEYE GFYRRGFFVT KKKYAVIDEE
610 620 630 640 650
DKITTRGLEI VRRDWSEIAK ETQARVLEAI LKHGDVEEAV RIVKEVTEKL
660 670 680 690 700
SRHEVPPEKL VIYEQITRDL RSYRATGPHV AVAKRLAARG IKIRPGTVIS
710 720 730 740 750
YIVLKGPGRV GDRAIPFDEF DPAKHRYDAE YYIENQVLPA VERILRAFGY
760 770
RKEDLRYQKT KQAGLGAWLK PKT
Length:773
Mass (Da):89,989
Last modified:December 15, 2003 - v1
Checksum:i03F7377635283A44
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti146 – 1549YHEGEEFGE → AHAGAAAGA(PubMed:10545321)Curated
Sequence conflicti287 – 2871K → A(PubMed:10545321)Curated
Sequence conflicti297 – 2971R → E(PubMed:10545321)Curated
Sequence conflicti300 – 3001E → A(PubMed:10545321)Curated
Sequence conflicti665 – 67612QITRD…SYRAT → A(PubMed:10545321)CuratedAdd
BLAST
Sequence conflicti682 – 69615VAKRL…KIRPG → AAA(PubMed:10545321)CuratedAdd
BLAST

Cross-referencesi

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1D5A X-ray 2.40 A 1-756 [» ]
1QQC X-ray 2.60 A 1-773 [» ]
ProteinModelPortali Q7SIG7.
SMRi Q7SIG7. Positions 1-756.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q7SIG7.

Family and domain databases

Gene3Di 3.30.420.10. 1 hit.
3.90.1600.10. 2 hits.
InterProi IPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR012337. RNaseH-like_dom.
[Graphical view ]
Pfami PF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 2 hits.
[Graphical view ]
PRINTSi PR00106. DNAPOLB.
SMARTi SM00486. POLBc. 1 hit.
[Graphical view ]
SUPFAMi SSF53098. SSF53098. 1 hit.
PROSITEi PS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Archaebacterial DNA polymerases tightly bind uracil-containing DNA."
    Lasken R.S., Schuster D.M., Rashtchian A.
    J. Biol. Chem. 271:17692-17696(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION.
  2. "Crystal structure of an archaebacterial DNA polymerase."
    Zhao Y., Jeruzalmi D., Moarefi I., Leighton L., Lasken R.S., Kuriyan J.
    Structure 7:1189-1199(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH MAGNESIUM, COFACTOR, DISULFIDE BONDS.

Entry informationi

Entry nameiDPOL_DESST
AccessioniPrimary (citable) accession number: Q7SIG7
Secondary accession number(s): Q7SIG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: December 15, 2003
Last modified: October 1, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

There are conflicts between the sequence shown here and that from PDB 1D5A.Curated

Keywords - Technical termi

3D-structure, Multifunctional enzyme

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3