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Q7SIG7

- DPOL_DESST

UniProt

Q7SIG7 - DPOL_DESST

Protein

DNA polymerase

Gene

pol

Organism
Desulfurococcus sp. (strain Tok)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (15 Dec 2003)
      Previous versions | rss
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    Functioni

    Thermostable DNA polymerase. In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity.1 Publication

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).1 Publication

    Cofactori

    Binds 2 magnesium ions.1 Publication

    Enzyme regulationi

    DNA polymerase activity strongly inhibited by uracil-containing oligonucleotides.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi141 – 1411Magnesium 11 Publication
    Metal bindingi141 – 1411Magnesium 21 Publication
    Metal bindingi143 – 1431Magnesium 21 Publication
    Metal bindingi315 – 3151Magnesium 11 Publication
    Metal bindingi315 – 3151Magnesium 21 Publication

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
    3. exonuclease activity Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW
    5. nucleotide binding Source: InterPro

    Keywords - Molecular functioni

    DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase (EC:2.7.7.7)
    Alternative name(s):
    D Tok Pol
    Gene namesi
    Name:pol
    OrganismiDesulfurococcus sp. (strain Tok)
    Taxonomic identifieri108142 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeDesulfurococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 773773DNA polymerasePRO_0000278146Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi428 ↔ 4421 Publication
    Disulfide bondi506 ↔ 5091 Publication

    Keywords - PTMi

    Disulfide bond

    Structurei

    Secondary structure

    1
    773
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 109
    Beta strandi13 – 2210
    Beta strandi25 – 317
    Beta strandi37 – 448
    Helixi45 – 473
    Turni49 – 535
    Beta strandi55 – 584
    Beta strandi61 – 644
    Beta strandi67 – 8620
    Helixi92 – 1009
    Beta strandi106 – 1116
    Helixi116 – 1238
    Beta strandi137 – 1437
    Beta strandi159 – 1646
    Beta strandi167 – 1748
    Beta strandi181 – 1833
    Helixi187 – 20115
    Beta strandi204 – 2107
    Turni211 – 2144
    Helixi215 – 22410
    Turni225 – 2273
    Beta strandi240 – 25112
    Beta strandi255 – 2595
    Helixi260 – 2678
    Helixi275 – 2828
    Helixi294 – 3007
    Helixi302 – 3054
    Helixi308 – 33730
    Helixi341 – 3444
    Helixi349 – 36315
    Helixi374 – 3807
    Beta strandi397 – 40711
    Helixi408 – 4158
    Helixi420 – 4223
    Beta strandi429 – 4335
    Turni435 – 4373
    Beta strandi440 – 4423
    Helixi448 – 46922
    Helixi473 – 49018
    Helixi493 – 4975
    Helixi507 – 53226
    Beta strandi535 – 5395
    Beta strandi541 – 5477
    Helixi553 – 56715
    Helixi568 – 5703
    Beta strandi576 – 59015
    Beta strandi593 – 5975
    Beta strandi603 – 6075
    Helixi617 – 63115
    Helixi636 – 65015
    Turni651 – 6533
    Helixi657 – 6593
    Beta strandi662 – 6643
    Helixi678 – 6825
    Turni687 – 6904
    Beta strandi699 – 7024
    Beta strandi709 – 7113
    Beta strandi717 – 7193
    Turni722 – 7243
    Turni729 – 7324
    Helixi733 – 7375
    Helixi738 – 7469
    Turni747 – 7493
    Helixi752 – 7543

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1D5AX-ray2.40A1-756[»]
    1QQCX-ray2.60A1-773[»]
    ProteinModelPortaliQ7SIG7.
    SMRiQ7SIG7. Positions 1-756.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ7SIG7.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 131131N-terminal domain1 PublicationAdd
    BLAST
    Regioni133 – 385253Exonuclease domain1 PublicationAdd
    BLAST
    Regioni390 – 773384Polymerase domain1 PublicationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the DNA polymerase type-B family.Sequence Analysis

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    3.90.1600.10. 2 hits.
    InterProiIPR006172. DNA-dir_DNA_pol_B.
    IPR017964. DNA-dir_DNA_pol_B_CS.
    IPR006133. DNA-dir_DNA_pol_B_exonuc.
    IPR006134. DNA-dir_DNA_pol_B_multi_dom.
    IPR023211. DNA_pol_palm_dom.
    IPR012337. RNaseH-like_dom.
    [Graphical view]
    PfamiPF00136. DNA_pol_B. 1 hit.
    PF03104. DNA_pol_B_exo1. 2 hits.
    [Graphical view]
    PRINTSiPR00106. DNAPOLB.
    SMARTiSM00486. POLBc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53098. SSF53098. 1 hit.
    PROSITEiPS00116. DNA_POLYMERASE_B. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7SIG7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MILDADYITE DGKPVIRVFK KEKGEFKIDY DRDFEPYIYA LLKDDSAIED    50
    IKKITAERHG TTVRVTRAER VKKKFLGRPV EVWKLYFTHP QDVPAIRDKI 100
    REHPAVVDIY EYDIPFAKRY LIDRGLIPME GDEELRMLAF DIETLYHEGE 150
    EFGEGPILMI SYADEEGARV ITWKNIDLPY VESVSTEKEM IKRFLKVIQE 200
    KDPDVLITYN GDNFDFAYLK KRSEMLGVKF ILGRDGSEPK IQRMGDRFAV 250
    EVKGRIHFDL YPVIRRTINL PTYTLETVYE PVFGQPKEKV YAEEIARAWE 300
    SGEGLERVAR YSMEDAKATY ELGKEFFPME AQLSRLVGQS LWDVSRSSTG 350
    NLVEWFLLRK AYERNDVAPN KPDERELARR TESYAGGYVK EPEKGLWENI 400
    VYLDYKSLYP SIIITHNVSP DTLNREGCRE YDVAPQVGHR FCKDFPGFIP 450
    SLLGDLLEER QKVKKKMKAT VDPIERKLLD YRQRAIKILA NSYYGYYAYA 500
    NARWYCRECA ESVTAWGRQY IETTMREIEE KFGFKVLYAD TDGFFATIPG 550
    ADAETVKNKA KEFLNYINPR LPGLLELEYE GFYRRGFFVT KKKYAVIDEE 600
    DKITTRGLEI VRRDWSEIAK ETQARVLEAI LKHGDVEEAV RIVKEVTEKL 650
    SRHEVPPEKL VIYEQITRDL RSYRATGPHV AVAKRLAARG IKIRPGTVIS 700
    YIVLKGPGRV GDRAIPFDEF DPAKHRYDAE YYIENQVLPA VERILRAFGY 750
    RKEDLRYQKT KQAGLGAWLK PKT 773
    Length:773
    Mass (Da):89,989
    Last modified:December 15, 2003 - v1
    Checksum:i03F7377635283A44
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti146 – 1549YHEGEEFGE → AHAGAAAGA(PubMed:10545321)Curated
    Sequence conflicti287 – 2871K → A(PubMed:10545321)Curated
    Sequence conflicti297 – 2971R → E(PubMed:10545321)Curated
    Sequence conflicti300 – 3001E → A(PubMed:10545321)Curated
    Sequence conflicti665 – 67612QITRD…SYRAT → A(PubMed:10545321)CuratedAdd
    BLAST
    Sequence conflicti682 – 69615VAKRL…KIRPG → AAA(PubMed:10545321)CuratedAdd
    BLAST

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1D5A X-ray 2.40 A 1-756 [» ]
    1QQC X-ray 2.60 A 1-773 [» ]
    ProteinModelPortali Q7SIG7.
    SMRi Q7SIG7. Positions 1-756.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q7SIG7.

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    3.90.1600.10. 2 hits.
    InterProi IPR006172. DNA-dir_DNA_pol_B.
    IPR017964. DNA-dir_DNA_pol_B_CS.
    IPR006133. DNA-dir_DNA_pol_B_exonuc.
    IPR006134. DNA-dir_DNA_pol_B_multi_dom.
    IPR023211. DNA_pol_palm_dom.
    IPR012337. RNaseH-like_dom.
    [Graphical view ]
    Pfami PF00136. DNA_pol_B. 1 hit.
    PF03104. DNA_pol_B_exo1. 2 hits.
    [Graphical view ]
    PRINTSi PR00106. DNAPOLB.
    SMARTi SM00486. POLBc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53098. SSF53098. 1 hit.
    PROSITEi PS00116. DNA_POLYMERASE_B. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Archaebacterial DNA polymerases tightly bind uracil-containing DNA."
      Lasken R.S., Schuster D.M., Rashtchian A.
      J. Biol. Chem. 271:17692-17696(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME REGULATION.
    2. "Crystal structure of an archaebacterial DNA polymerase."
      Zhao Y., Jeruzalmi D., Moarefi I., Leighton L., Lasken R.S., Kuriyan J.
      Structure 7:1189-1199(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH MAGNESIUM, COFACTOR, DISULFIDE BONDS.

    Entry informationi

    Entry nameiDPOL_DESST
    AccessioniPrimary (citable) accession number: Q7SIG7
    Secondary accession number(s): Q7SIG8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 20, 2007
    Last sequence update: December 15, 2003
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    There are conflicts between the sequence shown here and that from PDB 1D5A.Curated

    Keywords - Technical termi

    3D-structure, Multifunctional enzyme

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3