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Q7SIG7 (DPOL_DESST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase

EC=2.7.7.7
Alternative name(s):
D Tok Pol
Gene names
Name:pol
OrganismDesulfurococcus sp. (strain Tok)
Taxonomic identifier108142 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeDesulfurococcus

Protein attributes

Sequence length773 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Thermostable DNA polymerase. In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity. Ref.1

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). Ref.1

Cofactor

Binds 2 magnesium ions. Ref.2

Enzyme regulation

DNA polymerase activity strongly inhibited by uracil-containing oligonucleotides. Ref.1

Sequence similarities

Belongs to the DNA polymerase type-B family.

Caution

There are conflicts between the sequence shown here and that from PDB 1D5A.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 773773DNA polymerase
PRO_0000278146

Regions

Region1 – 131131N-terminal domain Ref.2
Region133 – 385253Exonuclease domain Ref.2
Region390 – 773384Polymerase domain Ref.2

Sites

Metal binding1411Magnesium 1 Probable Ref.2
Metal binding1411Magnesium 2 Probable Ref.2
Metal binding1431Magnesium 2 Probable Ref.2
Metal binding3151Magnesium 1 Probable Ref.2
Metal binding3151Magnesium 2 Probable Ref.2

Amino acid modifications

Disulfide bond428 ↔ 442 Ref.2
Disulfide bond506 ↔ 509 Ref.2

Experimental info

Sequence conflict146 – 1549YHEGEEFGE → AHAGAAAGA Ref.2
Sequence conflict2871K → A Ref.2
Sequence conflict2971R → E Ref.2
Sequence conflict3001E → A Ref.2
Sequence conflict665 – 67612QITRD…SYRAT → A Ref.2
Sequence conflict682 – 69615VAKRL…KIRPG → AAA Ref.2

Secondary structure

...................................................................................................................... 773
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q7SIG7 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: 03F7377635283A44

FASTA77389,989
        10         20         30         40         50         60 
MILDADYITE DGKPVIRVFK KEKGEFKIDY DRDFEPYIYA LLKDDSAIED IKKITAERHG 

        70         80         90        100        110        120 
TTVRVTRAER VKKKFLGRPV EVWKLYFTHP QDVPAIRDKI REHPAVVDIY EYDIPFAKRY 

       130        140        150        160        170        180 
LIDRGLIPME GDEELRMLAF DIETLYHEGE EFGEGPILMI SYADEEGARV ITWKNIDLPY 

       190        200        210        220        230        240 
VESVSTEKEM IKRFLKVIQE KDPDVLITYN GDNFDFAYLK KRSEMLGVKF ILGRDGSEPK 

       250        260        270        280        290        300 
IQRMGDRFAV EVKGRIHFDL YPVIRRTINL PTYTLETVYE PVFGQPKEKV YAEEIARAWE 

       310        320        330        340        350        360 
SGEGLERVAR YSMEDAKATY ELGKEFFPME AQLSRLVGQS LWDVSRSSTG NLVEWFLLRK 

       370        380        390        400        410        420 
AYERNDVAPN KPDERELARR TESYAGGYVK EPEKGLWENI VYLDYKSLYP SIIITHNVSP 

       430        440        450        460        470        480 
DTLNREGCRE YDVAPQVGHR FCKDFPGFIP SLLGDLLEER QKVKKKMKAT VDPIERKLLD 

       490        500        510        520        530        540 
YRQRAIKILA NSYYGYYAYA NARWYCRECA ESVTAWGRQY IETTMREIEE KFGFKVLYAD 

       550        560        570        580        590        600 
TDGFFATIPG ADAETVKNKA KEFLNYINPR LPGLLELEYE GFYRRGFFVT KKKYAVIDEE 

       610        620        630        640        650        660 
DKITTRGLEI VRRDWSEIAK ETQARVLEAI LKHGDVEEAV RIVKEVTEKL SRHEVPPEKL 

       670        680        690        700        710        720 
VIYEQITRDL RSYRATGPHV AVAKRLAARG IKIRPGTVIS YIVLKGPGRV GDRAIPFDEF 

       730        740        750        760        770 
DPAKHRYDAE YYIENQVLPA VERILRAFGY RKEDLRYQKT KQAGLGAWLK PKT 

« Hide

References

[1]"Archaebacterial DNA polymerases tightly bind uracil-containing DNA."
Lasken R.S., Schuster D.M., Rashtchian A.
J. Biol. Chem. 271:17692-17696(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ENZYME REGULATION.
[2]"Crystal structure of an archaebacterial DNA polymerase."
Zhao Y., Jeruzalmi D., Moarefi I., Leighton L., Lasken R.S., Kuriyan J.
Structure 7:1189-1199(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH MAGNESIUM, COFACTOR, DISULFIDE BONDS.

Cross-references

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1D5AX-ray2.40A1-756[»]
1QQCX-ray2.60A1-773[»]
ProteinModelPortalQ7SIG7.
SMRQ7SIG7. Positions 1-756.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.420.10. 1 hit.
3.90.1600.10. 2 hits.
InterProIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR004578. DNA-dir_DNA_pol_B_pol2.
IPR023211. DNA_pol_palm_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 2 hits.
[Graphical view]
PRINTSPR00106. DNAPOLB.
SMARTSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00592. pol2. 2 hits.
PROSITEPS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ7SIG7.

Entry information

Entry nameDPOL_DESST
AccessionPrimary (citable) accession number: Q7SIG7
Secondary accession number(s): Q7SIG8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: December 15, 2003
Last modified: April 16, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references