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Protein

Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2

Gene

Asap2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN recruitment to focal contacts and cell migration (By similarity).By similarity2 Publications

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2
Alternative name(s):
Development and differentiation-enhancing factor 2
Paxillin-associated protein with ARF GAP activity 3
Short name:
PAG3
Pyk2 C-terminus-associated protein
Short name:
PAP
Gene namesi
Name:Asap2
Synonyms:Ddef2, Gm1523, Gm592
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2685438. Asap2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi449 – 4491W → A: Strongly reduces Arf-GAP mediated stimulation of GTP hydrolysis. 1 Publication
Mutagenesisi460 – 4601I → A: Reduces Arf-GAP mediated stimulation of GTP hydrolysis 100-fold and abolishes Arf-GAP mediated stimulation of GTP hydrolysis; when associated with A-306. 1 Publication
Mutagenesisi467 – 4671R → A: Abolishes Arf-GAP mediated stimulation of GTP hydrolysis. 1 Publication
Mutagenesisi467 – 4671R → K: Reduces Arf-GAP mediated stimulation of GTP hydrolysis more than 10000-fold. 1 Publication
Mutagenesisi481 – 4811L → A: Reduces Arf-GAP mediated stimulation of GTP hydrolysis 100-fold and abolishes Arf-GAP mediated stimulation of GTP hydrolysis; when associated with A-285. 1 Publication
Mutagenesisi482 – 4821D → A: Reduces Arf-GAP mediated stimulation of GTP hydrolysis 1000-fold. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 958958Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2PRO_0000074199Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei704 – 7041PhosphoserineCombined sources
Modified residuei861 – 8611PhosphothreonineBy similarity

Post-translational modificationi

Phosphorylated on tyrosine residues by SRC and PTK2B.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ7SIG6.
PeptideAtlasiQ7SIG6.
PRIDEiQ7SIG6.

PTM databases

iPTMnetiQ7SIG6.
PhosphoSiteiQ7SIG6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000052632.
ExpressionAtlasiQ7SIG6. baseline and differential.
GenevisibleiQ7SIG6. MM.

Interactioni

Subunit structurei

Binds PXN, ARF1, ARF5, ARF6, PTK2B and SRC.1 Publication

Protein-protein interaction databases

BioGridi229271. 2 interactions.
IntActiQ7SIG6. 2 interactions.

Structurei

Secondary structure

1
958
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi423 – 43210Combined sources
Turni434 – 4374Combined sources
Turni440 – 4423Combined sources
Beta strandi449 – 4513Combined sources
Turni452 – 4543Combined sources
Helixi460 – 46910Combined sources
Turni471 – 4733Combined sources
Beta strandi476 – 4783Combined sources
Turni479 – 4813Combined sources
Helixi486 – 4894Combined sources
Helixi490 – 4945Combined sources
Helixi497 – 5048Combined sources
Turni505 – 5073Combined sources
Beta strandi510 – 5123Combined sources
Helixi522 – 53312Combined sources
Beta strandi543 – 5453Combined sources
Helixi546 – 55813Combined sources
Helixi562 – 5709Combined sources
Helixi591 – 5988Combined sources
Turni601 – 6033Combined sources
Helixi604 – 61310Combined sources
Helixi627 – 6337Combined sources
Helixi637 – 6459Combined sources
Helixi660 – 6667Combined sources
Helixi670 – 68011Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DCQX-ray2.10A421-697[»]
ProteinModelPortaliQ7SIG6.
SMRiQ7SIG6. Positions 30-409, 422-697, 899-958.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7SIG6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini308 – 40093PHPROSITE-ProRule annotationAdd
BLAST
Domaini424 – 546123Arf-GAPPROSITE-ProRule annotationAdd
BLAST
Repeati587 – 61933ANK 1Add
BLAST
Repeati623 – 65533ANK 2Add
BLAST
Domaini896 – 95863SH3PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili259 – 28628Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi774 – 888115Pro-richAdd
BLAST

Domaini

The conserved Arg-467 in the Arf-GAP domain probably becomes part of the active site of bound small GTPases and is necessary for GTP hydrolysis.

Sequence similaritiesi

Contains 2 ANK repeats.PROSITE-ProRule annotation
Contains 1 Arf-GAP domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Coiled coil, Repeat, SH3 domain

Phylogenomic databases

GeneTreeiENSGT00760000118874.
HOGENOMiHOG000230570.
HOVERGENiHBG051327.
InParanoidiQ7SIG6.
KOiK12488.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
1.25.40.20. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF00169. PH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 3 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7SIG6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPDQISVSEF VAETHEDYKA PTASSFTTRT AQCRNTVAAI EEALDVDRMV
60 70 80 90 100
LYKMKKSVKA INISGLAHVE NEEQYTQALE KFGGNCVCRD DPDLGSAFLK
110 120 130 140 150
FSVFTKELTA LFKNLIQNMN NIISFPLDSL LKGDLKGVKG DLKKPFDKAW
160 170 180 190 200
KDYETKITKI EKEKKEHAKL HGMIRTEISG AEIAEEMEKE RRFFQLQMCE
210 220 230 240 250
YLLKVNEIKV KKGVDLLQNL IKYFHAQCNF FQDGLKAVES LKPSIETLST
260 270 280 290 300
DLHTIKQAQD EERRQLIQLR DILKSALQVE QKESRRDSQL RQSTAYSLHQ
310 320 330 340 350
PQGNKEHGTE RNGNLYKKSD GIRKVWQKRK CSVKNGFLTI SHGTANRPPA
360 370 380 390 400
KLNLLTCQVK TNPEEKKCFD LISHDRTYHF QAEDEQECQI WMSVLQNSKE
410 420 430 440 450
EALNNAFKGD DNTGENNIVQ ELTKEIISEV QRMTGNDVCC DCGAPDPTWL
460 470 480 490 500
STNLGILTCI ECSGIHRELG VHYSRMQSLT LDVLGTSELL LAKNIGNAGF
510 520 530 540 550
NEIMECCLPS EDPVKPNPGS DMIARKDYIT AKYMERRYAR KKHADTAAKL
560 570 580 590 600
HSLCEAVKTR DIFGLLQAYA DGVDLTEKIP LANGHEPDET ALHLAVRSVD
610 620 630 640 650
RTSLHIVDFL VQNSGNLDKQ TGKGSTALHY CCLTDNAECL KLLLRGKASI
660 670 680 690 700
EIANESGETP LDIAKRLKHE HCEELLTQAL SGRFNSHVHV EYEWRLLHED
710 720 730 740 750
LDESDDDVDE KLQPSPNRRE DRPVSFYQLG SSQFQSNAVS LARDTANLTK
760 770 780 790 800
DKQRGFGPSI LQNETYGAIL SGSPPSSQSI PPSTTSAPPL PPRNVGKDPL
810 820 830 840 850
TTTPPPPVAK TSGTLEAMNQ PSKSSQPGTS QSKPPPLPPQ PPSRLPQKKP
860 870 880 890 900
ASGTDKPTPL TNKGQPRGPE ASGPLSNAMA LQPPAPMPRK SQATKSKPKR
910 920 930 940 950
VKALYNCVAD NPDELTFSEG DVIIVDGEED QEWWIGHIDG EPSRKGAFPV

SFVHFIAD
Length:958
Mass (Da):106,805
Last modified:July 19, 2005 - v3
Checksum:i2CA0056C2AD14D7E
GO
Isoform 2 (identifier: Q7SIG6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     284-286: Missing.
     345-490: Missing.

Show »
Length:809
Mass (Da):90,022
Checksum:iB77505164F392CCA
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei284 – 2863Missing in isoform 2. 1 PublicationVSP_014776
Alternative sequencei345 – 490146Missing in isoform 2. 1 PublicationVSP_014777Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC096022 mRNA. Translation: AAH96022.1.
BC080847 mRNA. Translation: AAH80847.1.
CCDSiCCDS56832.1. [Q7SIG6-1]
RefSeqiNP_001004364.2. NM_001004364.2.
NP_001091637.1. NM_001098168.1. [Q7SIG6-1]
NP_001128664.1. NM_001135192.1.
UniGeneiMm.358946.

Genome annotation databases

EnsembliENSMUST00000090834; ENSMUSP00000088344; ENSMUSG00000052632. [Q7SIG6-2]
ENSMUST00000101562; ENSMUSP00000099098; ENSMUSG00000052632. [Q7SIG6-1]
GeneIDi211914.
KEGGimmu:211914.
UCSCiuc007ndi.1. mouse. [Q7SIG6-1]
uc011ykr.1. mouse. [Q7SIG6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC096022 mRNA. Translation: AAH96022.1.
BC080847 mRNA. Translation: AAH80847.1.
CCDSiCCDS56832.1. [Q7SIG6-1]
RefSeqiNP_001004364.2. NM_001004364.2.
NP_001091637.1. NM_001098168.1. [Q7SIG6-1]
NP_001128664.1. NM_001135192.1.
UniGeneiMm.358946.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DCQX-ray2.10A421-697[»]
ProteinModelPortaliQ7SIG6.
SMRiQ7SIG6. Positions 30-409, 422-697, 899-958.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229271. 2 interactions.
IntActiQ7SIG6. 2 interactions.

PTM databases

iPTMnetiQ7SIG6.
PhosphoSiteiQ7SIG6.

Proteomic databases

MaxQBiQ7SIG6.
PeptideAtlasiQ7SIG6.
PRIDEiQ7SIG6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000090834; ENSMUSP00000088344; ENSMUSG00000052632. [Q7SIG6-2]
ENSMUST00000101562; ENSMUSP00000099098; ENSMUSG00000052632. [Q7SIG6-1]
GeneIDi211914.
KEGGimmu:211914.
UCSCiuc007ndi.1. mouse. [Q7SIG6-1]
uc011ykr.1. mouse. [Q7SIG6-2]

Organism-specific databases

CTDi8853.
MGIiMGI:2685438. Asap2.

Phylogenomic databases

GeneTreeiENSGT00760000118874.
HOGENOMiHOG000230570.
HOVERGENiHBG051327.
InParanoidiQ7SIG6.
KOiK12488.

Miscellaneous databases

ChiTaRSiAsap2. mouse.
EvolutionaryTraceiQ7SIG6.
PROiQ7SIG6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000052632.
ExpressionAtlasiQ7SIG6. baseline and differential.
GenevisibleiQ7SIG6. MM.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
1.25.40.20. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF00169. PH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 3 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASAP2_MOUSE
AccessioniPrimary (citable) accession number: Q7SIG6
Secondary accession number(s): Q501K1, Q66JN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: July 19, 2005
Last modified: September 7, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.