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Protein

Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2

Gene

Asap2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN recruitment to focal contacts and cell migration (By similarity).By similarity2 Publications

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2
Alternative name(s):
Development and differentiation-enhancing factor 2
Paxillin-associated protein with ARF GAP activity 3
Short name:
PAG3
Pyk2 C-terminus-associated protein
Short name:
PAP
Gene namesi
Name:Asap2
Synonyms:Ddef2, Gm1523, Gm592
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2685438. Asap2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi449W → A: Strongly reduces Arf-GAP mediated stimulation of GTP hydrolysis. 1 Publication1
Mutagenesisi460I → A: Reduces Arf-GAP mediated stimulation of GTP hydrolysis 100-fold and abolishes Arf-GAP mediated stimulation of GTP hydrolysis; when associated with A-306. 1 Publication1
Mutagenesisi467R → A: Abolishes Arf-GAP mediated stimulation of GTP hydrolysis. 1 Publication1
Mutagenesisi467R → K: Reduces Arf-GAP mediated stimulation of GTP hydrolysis more than 10000-fold. 1 Publication1
Mutagenesisi481L → A: Reduces Arf-GAP mediated stimulation of GTP hydrolysis 100-fold and abolishes Arf-GAP mediated stimulation of GTP hydrolysis; when associated with A-285. 1 Publication1
Mutagenesisi482D → A: Reduces Arf-GAP mediated stimulation of GTP hydrolysis 1000-fold. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000741991 – 958Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2Add BLAST958

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei704PhosphoserineCombined sources1
Modified residuei861PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues by SRC and PTK2B.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PeptideAtlasiQ7SIG6.
PRIDEiQ7SIG6.

PTM databases

iPTMnetiQ7SIG6.
PhosphoSitePlusiQ7SIG6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000052632.
ExpressionAtlasiQ7SIG6. baseline and differential.
GenevisibleiQ7SIG6. MM.

Interactioni

Subunit structurei

Binds PXN, ARF1, ARF5, ARF6, PTK2B and SRC.1 Publication

Protein-protein interaction databases

BioGridi229271. 2 interactors.
IntActiQ7SIG6. 2 interactors.

Structurei

Secondary structure

1958
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi423 – 432Combined sources10
Turni434 – 437Combined sources4
Turni440 – 442Combined sources3
Beta strandi449 – 451Combined sources3
Turni452 – 454Combined sources3
Helixi460 – 469Combined sources10
Turni471 – 473Combined sources3
Beta strandi476 – 478Combined sources3
Turni479 – 481Combined sources3
Helixi486 – 489Combined sources4
Helixi490 – 494Combined sources5
Helixi497 – 504Combined sources8
Turni505 – 507Combined sources3
Beta strandi510 – 512Combined sources3
Helixi522 – 533Combined sources12
Beta strandi543 – 545Combined sources3
Helixi546 – 558Combined sources13
Helixi562 – 570Combined sources9
Helixi591 – 598Combined sources8
Turni601 – 603Combined sources3
Helixi604 – 613Combined sources10
Helixi627 – 633Combined sources7
Helixi637 – 645Combined sources9
Helixi660 – 666Combined sources7
Helixi670 – 680Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DCQX-ray2.10A421-697[»]
ProteinModelPortaliQ7SIG6.
SMRiQ7SIG6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7SIG6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini308 – 400PHPROSITE-ProRule annotationAdd BLAST93
Domaini424 – 546Arf-GAPPROSITE-ProRule annotationAdd BLAST123
Repeati587 – 619ANK 1Add BLAST33
Repeati623 – 655ANK 2Add BLAST33
Domaini896 – 958SH3PROSITE-ProRule annotationAdd BLAST63

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili259 – 286Sequence analysisAdd BLAST28

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi774 – 888Pro-richAdd BLAST115

Domaini

The conserved Arg-467 in the Arf-GAP domain probably becomes part of the active site of bound small GTPases and is necessary for GTP hydrolysis.

Sequence similaritiesi

Contains 2 ANK repeats.PROSITE-ProRule annotation
Contains 1 Arf-GAP domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Coiled coil, Repeat, SH3 domain

Phylogenomic databases

GeneTreeiENSGT00760000118874.
HOGENOMiHOG000230570.
HOVERGENiHBG051327.
InParanoidiQ7SIG6.
KOiK12488.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
1.25.40.20. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF00169. PH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 3 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7SIG6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPDQISVSEF VAETHEDYKA PTASSFTTRT AQCRNTVAAI EEALDVDRMV
60 70 80 90 100
LYKMKKSVKA INISGLAHVE NEEQYTQALE KFGGNCVCRD DPDLGSAFLK
110 120 130 140 150
FSVFTKELTA LFKNLIQNMN NIISFPLDSL LKGDLKGVKG DLKKPFDKAW
160 170 180 190 200
KDYETKITKI EKEKKEHAKL HGMIRTEISG AEIAEEMEKE RRFFQLQMCE
210 220 230 240 250
YLLKVNEIKV KKGVDLLQNL IKYFHAQCNF FQDGLKAVES LKPSIETLST
260 270 280 290 300
DLHTIKQAQD EERRQLIQLR DILKSALQVE QKESRRDSQL RQSTAYSLHQ
310 320 330 340 350
PQGNKEHGTE RNGNLYKKSD GIRKVWQKRK CSVKNGFLTI SHGTANRPPA
360 370 380 390 400
KLNLLTCQVK TNPEEKKCFD LISHDRTYHF QAEDEQECQI WMSVLQNSKE
410 420 430 440 450
EALNNAFKGD DNTGENNIVQ ELTKEIISEV QRMTGNDVCC DCGAPDPTWL
460 470 480 490 500
STNLGILTCI ECSGIHRELG VHYSRMQSLT LDVLGTSELL LAKNIGNAGF
510 520 530 540 550
NEIMECCLPS EDPVKPNPGS DMIARKDYIT AKYMERRYAR KKHADTAAKL
560 570 580 590 600
HSLCEAVKTR DIFGLLQAYA DGVDLTEKIP LANGHEPDET ALHLAVRSVD
610 620 630 640 650
RTSLHIVDFL VQNSGNLDKQ TGKGSTALHY CCLTDNAECL KLLLRGKASI
660 670 680 690 700
EIANESGETP LDIAKRLKHE HCEELLTQAL SGRFNSHVHV EYEWRLLHED
710 720 730 740 750
LDESDDDVDE KLQPSPNRRE DRPVSFYQLG SSQFQSNAVS LARDTANLTK
760 770 780 790 800
DKQRGFGPSI LQNETYGAIL SGSPPSSQSI PPSTTSAPPL PPRNVGKDPL
810 820 830 840 850
TTTPPPPVAK TSGTLEAMNQ PSKSSQPGTS QSKPPPLPPQ PPSRLPQKKP
860 870 880 890 900
ASGTDKPTPL TNKGQPRGPE ASGPLSNAMA LQPPAPMPRK SQATKSKPKR
910 920 930 940 950
VKALYNCVAD NPDELTFSEG DVIIVDGEED QEWWIGHIDG EPSRKGAFPV

SFVHFIAD
Length:958
Mass (Da):106,805
Last modified:July 19, 2005 - v3
Checksum:i2CA0056C2AD14D7E
GO
Isoform 2 (identifier: Q7SIG6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     284-286: Missing.
     345-490: Missing.

Show »
Length:809
Mass (Da):90,022
Checksum:iB77505164F392CCA
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014776284 – 286Missing in isoform 2. 1 Publication3
Alternative sequenceiVSP_014777345 – 490Missing in isoform 2. 1 PublicationAdd BLAST146

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC096022 mRNA. Translation: AAH96022.1.
BC080847 mRNA. Translation: AAH80847.1.
CCDSiCCDS56832.1. [Q7SIG6-1]
RefSeqiNP_001004364.2. NM_001004364.2.
NP_001091637.1. NM_001098168.1. [Q7SIG6-1]
NP_001128664.1. NM_001135192.1.
UniGeneiMm.358946.

Genome annotation databases

EnsembliENSMUST00000090834; ENSMUSP00000088344; ENSMUSG00000052632. [Q7SIG6-2]
ENSMUST00000101562; ENSMUSP00000099098; ENSMUSG00000052632. [Q7SIG6-1]
GeneIDi211914.
KEGGimmu:211914.
UCSCiuc007ndi.1. mouse. [Q7SIG6-1]
uc011ykr.1. mouse. [Q7SIG6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC096022 mRNA. Translation: AAH96022.1.
BC080847 mRNA. Translation: AAH80847.1.
CCDSiCCDS56832.1. [Q7SIG6-1]
RefSeqiNP_001004364.2. NM_001004364.2.
NP_001091637.1. NM_001098168.1. [Q7SIG6-1]
NP_001128664.1. NM_001135192.1.
UniGeneiMm.358946.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DCQX-ray2.10A421-697[»]
ProteinModelPortaliQ7SIG6.
SMRiQ7SIG6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229271. 2 interactors.
IntActiQ7SIG6. 2 interactors.

PTM databases

iPTMnetiQ7SIG6.
PhosphoSitePlusiQ7SIG6.

Proteomic databases

PeptideAtlasiQ7SIG6.
PRIDEiQ7SIG6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000090834; ENSMUSP00000088344; ENSMUSG00000052632. [Q7SIG6-2]
ENSMUST00000101562; ENSMUSP00000099098; ENSMUSG00000052632. [Q7SIG6-1]
GeneIDi211914.
KEGGimmu:211914.
UCSCiuc007ndi.1. mouse. [Q7SIG6-1]
uc011ykr.1. mouse. [Q7SIG6-2]

Organism-specific databases

CTDi8853.
MGIiMGI:2685438. Asap2.

Phylogenomic databases

GeneTreeiENSGT00760000118874.
HOGENOMiHOG000230570.
HOVERGENiHBG051327.
InParanoidiQ7SIG6.
KOiK12488.

Miscellaneous databases

ChiTaRSiAsap2. mouse.
EvolutionaryTraceiQ7SIG6.
PROiQ7SIG6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000052632.
ExpressionAtlasiQ7SIG6. baseline and differential.
GenevisibleiQ7SIG6. MM.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
1.25.40.20. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF00169. PH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 3 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASAP2_MOUSE
AccessioniPrimary (citable) accession number: Q7SIG6
Secondary accession number(s): Q501K1, Q66JN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: July 19, 2005
Last modified: November 2, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.