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Q7SIG5

- GUN6_HUMIN

UniProt

Q7SIG5 - GUN6_HUMIN

Protein

Endoglucanase-6B

Gene

cel6B

Organism
Humicola insolens (Soft-rot fungus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (15 Dec 2003)
      Previous versions | rss
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    Functioni

    Plays a central role in the recycling of plant biomass. The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the dissaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei52 – 521SubstrateBy similarity
    Binding sitei54 – 541SubstrateBy similarity
    Active sitei92 – 921Proton donor1 PublicationPROSITE-ProRule annotation
    Active sitei139 – 1391Proton donor1 PublicationPROSITE-ProRule annotation
    Binding sitei183 – 1831SubstrateBy similarity
    Binding sitei186 – 1861SubstrateBy similarity
    Binding sitei222 – 2221SubstrateBy similarity
    Binding sitei282 – 2821SubstrateBy similarity
    Binding sitei310 – 3101SubstrateBy similarity
    Binding sitei314 – 3141SubstrateBy similarity

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-17625.

    Protein family/group databases

    CAZyiGH6. Glycoside Hydrolase Family 6.
    mycoCLAPiEGL6B_HUMIN.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase-6B (EC:3.2.1.4)
    Alternative name(s):
    Cellulase 6B
    Endo-1,4-beta-glucanase 6B
    Gene namesi
    Name:cel6B1 Publication
    OrganismiHumicola insolens (Soft-rot fungus)
    Taxonomic identifieri34413 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesChaetomiaceaeHumicola

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 348348Endoglucanase-6BPRO_0000248842Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Structurei

    Secondary structure

    1
    348
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni5 – 84
    Helixi15 – 2915
    Turni30 – 323
    Helixi34 – 4613
    Beta strandi51 – 533
    Helixi57 – 593
    Helixi60 – 7415
    Beta strandi79 – 857
    Helixi93 – 953
    Beta strandi99 – 1013
    Helixi104 – 1063
    Helixi108 – 1158
    Helixi117 – 12610
    Beta strandi132 – 1365
    Helixi140 – 1467
    Helixi150 – 16920
    Beta strandi175 – 1806
    Helixi184 – 1874
    Helixi190 – 1923
    Helixi193 – 20715
    Beta strandi215 – 2184
    Helixi233 – 2353
    Helixi243 – 25513
    Turni256 – 2583
    Beta strandi262 – 2665
    Beta strandi268 – 2714
    Beta strandi286 – 2894
    Beta strandi294 – 2963
    Beta strandi303 – 3075
    Beta strandi316 – 3183
    Helixi332 – 3409

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DYSX-ray1.60A/B1-348[»]
    ProteinModelPortaliQ7SIG5.
    SMRiQ7SIG5. Positions 3-347.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ7SIG5.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 6 (cellulase B) family.Sequence Analysis

    Family and domain databases

    Gene3Di3.20.20.40. 1 hit.
    InterProiIPR016288. Beta_cellobiohydrolase.
    IPR001524. Glyco_hydro_6_CS.
    [Graphical view]
    PfamiPF01341. Glyco_hydro_6. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001100. Beta_cellobiohydrolase. 1 hit.
    PRINTSiPR00733. GLHYDRLASE6.
    SUPFAMiSSF51989. SSF51989. 1 hit.
    PROSITEiPS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7SIG5-1 [UniParc]FASTAAdd to Basket

    « Hide

    QSGNPFSGRT LLVNSDYSSK LDQTRQAFLS RGDQTNAAKV KYVQEKVGTF    50
    YWISNIFLLR DIDVAIQNAR AAKARGENPI VGLVLYNLPD RDCSAGESSG 100
    ELKLSQNGLN RYKNEYVNPF AQKLKAASDV QFAVILEPDA IGNMVTGTSA 150
    FCRNARGPQQ EAIGYAISQL QASHIHLYLD VANGGWLGWA DKLEPTAQEV 200
    ATILQKAGNN AKIRGFSSNV SNYNPYSTSN PPPYTSGSPS PDESRYATNI 250
    ANAMRQRGLP TQFIIDQSRV ALSGARSEWG QWCNVNPAGF GQPFTTNTNN 300
    PNVDAIVWVK PGGESDGQCG MGGAPAAGMW FDAYAQMLTQ NAHDEIAR 348
    Length:348
    Mass (Da):37,753
    Last modified:December 15, 2003 - v1
    Checksum:i5F1E4A4367402E54
    GO

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DYS X-ray 1.60 A/B 1-348 [» ]
    ProteinModelPortali Q7SIG5.
    SMRi Q7SIG5. Positions 3-347.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH6. Glycoside Hydrolase Family 6.
    mycoCLAPi EGL6B_HUMIN.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-17625.

    Miscellaneous databases

    EvolutionaryTracei Q7SIG5.

    Family and domain databases

    Gene3Di 3.20.20.40. 1 hit.
    InterProi IPR016288. Beta_cellobiohydrolase.
    IPR001524. Glyco_hydro_6_CS.
    [Graphical view ]
    Pfami PF01341. Glyco_hydro_6. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001100. Beta_cellobiohydrolase. 1 hit.
    PRINTSi PR00733. GLHYDRLASE6.
    SUPFAMi SSF51989. SSF51989. 1 hit.
    PROSITEi PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Enzymatic properties of cellulases from Humicola insolens."
      Schuelein M.
      J. Biotechnol. 57:71-81(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY.
    2. "Structure and function of Humicola insolens family 6 cellulases: structure of the endoglucanase, Cel6B, at 1.6 A resolution."
      Davies G.J., Brzozowski A.M., Dauter M., Varrot A., Schuelein M.
      Biochem. J. 348:201-207(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS), SUBUNIT, ACTIVE SITE.

    Entry informationi

    Entry nameiGUN6_HUMIN
    AccessioniPrimary (citable) accession number: Q7SIG5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: December 15, 2003
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3