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Q7SIG5

- GUN6_HUMIN

UniProt

Q7SIG5 - GUN6_HUMIN

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Protein

Endoglucanase-6B

Gene

cel6B

Organism
Humicola insolens (Soft-rot fungus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Plays a central role in the recycling of plant biomass. The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the dissaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei52 – 521SubstrateBy similarity
Binding sitei54 – 541SubstrateBy similarity
Active sitei92 – 921Proton donor1 PublicationPROSITE-ProRule annotation
Active sitei139 – 1391Proton donor1 PublicationPROSITE-ProRule annotation
Binding sitei183 – 1831SubstrateBy similarity
Binding sitei186 – 1861SubstrateBy similarity
Binding sitei222 – 2221SubstrateBy similarity
Binding sitei282 – 2821SubstrateBy similarity
Binding sitei310 – 3101SubstrateBy similarity
Binding sitei314 – 3141SubstrateBy similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17625.

Protein family/group databases

CAZyiGH6. Glycoside Hydrolase Family 6.
mycoCLAPiEGL6B_HUMIN.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase-6B (EC:3.2.1.4)
Alternative name(s):
Cellulase 6B
Endo-1,4-beta-glucanase 6B
Gene namesi
Name:cel6B1 Publication
OrganismiHumicola insolens (Soft-rot fungus)
Taxonomic identifieri34413 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesChaetomiaceaeHumicola

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 348348Endoglucanase-6BPRO_0000248842Add
BLAST

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

Secondary structure

1
348
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni5 – 84
Helixi15 – 2915
Turni30 – 323
Helixi34 – 4613
Beta strandi51 – 533
Helixi57 – 593
Helixi60 – 7415
Beta strandi79 – 857
Helixi93 – 953
Beta strandi99 – 1013
Helixi104 – 1063
Helixi108 – 1158
Helixi117 – 12610
Beta strandi132 – 1365
Helixi140 – 1467
Helixi150 – 16920
Beta strandi175 – 1806
Helixi184 – 1874
Helixi190 – 1923
Helixi193 – 20715
Beta strandi215 – 2184
Helixi233 – 2353
Helixi243 – 25513
Turni256 – 2583
Beta strandi262 – 2665
Beta strandi268 – 2714
Beta strandi286 – 2894
Beta strandi294 – 2963
Beta strandi303 – 3075
Beta strandi316 – 3183
Helixi332 – 3409

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DYSX-ray1.60A/B1-348[»]
ProteinModelPortaliQ7SIG5.
SMRiQ7SIG5. Positions 3-347.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7SIG5.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 6 (cellulase B) family.Sequence Analysis

Family and domain databases

Gene3Di3.20.20.40. 1 hit.
InterProiIPR016288. Beta_cellobiohydrolase.
IPR001524. Glyco_hydro_6_CS.
[Graphical view]
PfamiPF01341. Glyco_hydro_6. 1 hit.
[Graphical view]
PIRSFiPIRSF001100. Beta_cellobiohydrolase. 1 hit.
PRINTSiPR00733. GLHYDRLASE6.
SUPFAMiSSF51989. SSF51989. 1 hit.
PROSITEiPS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7SIG5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
QSGNPFSGRT LLVNSDYSSK LDQTRQAFLS RGDQTNAAKV KYVQEKVGTF
60 70 80 90 100
YWISNIFLLR DIDVAIQNAR AAKARGENPI VGLVLYNLPD RDCSAGESSG
110 120 130 140 150
ELKLSQNGLN RYKNEYVNPF AQKLKAASDV QFAVILEPDA IGNMVTGTSA
160 170 180 190 200
FCRNARGPQQ EAIGYAISQL QASHIHLYLD VANGGWLGWA DKLEPTAQEV
210 220 230 240 250
ATILQKAGNN AKIRGFSSNV SNYNPYSTSN PPPYTSGSPS PDESRYATNI
260 270 280 290 300
ANAMRQRGLP TQFIIDQSRV ALSGARSEWG QWCNVNPAGF GQPFTTNTNN
310 320 330 340
PNVDAIVWVK PGGESDGQCG MGGAPAAGMW FDAYAQMLTQ NAHDEIAR
Length:348
Mass (Da):37,753
Last modified:December 15, 2003 - v1
Checksum:i5F1E4A4367402E54
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DYS X-ray 1.60 A/B 1-348 [» ]
ProteinModelPortali Q7SIG5.
SMRi Q7SIG5. Positions 3-347.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH6. Glycoside Hydrolase Family 6.
mycoCLAPi EGL6B_HUMIN.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-17625.

Miscellaneous databases

EvolutionaryTracei Q7SIG5.

Family and domain databases

Gene3Di 3.20.20.40. 1 hit.
InterProi IPR016288. Beta_cellobiohydrolase.
IPR001524. Glyco_hydro_6_CS.
[Graphical view ]
Pfami PF01341. Glyco_hydro_6. 1 hit.
[Graphical view ]
PIRSFi PIRSF001100. Beta_cellobiohydrolase. 1 hit.
PRINTSi PR00733. GLHYDRLASE6.
SUPFAMi SSF51989. SSF51989. 1 hit.
PROSITEi PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Enzymatic properties of cellulases from Humicola insolens."
    Schuelein M.
    J. Biotechnol. 57:71-81(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY.
  2. "Structure and function of Humicola insolens family 6 cellulases: structure of the endoglucanase, Cel6B, at 1.6 A resolution."
    Davies G.J., Brzozowski A.M., Dauter M., Varrot A., Schuelein M.
    Biochem. J. 348:201-207(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS), SUBUNIT, ACTIVE SITE.

Entry informationi

Entry nameiGUN6_HUMIN
AccessioniPrimary (citable) accession number: Q7SIG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: December 15, 2003
Last modified: October 1, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3