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Protein

Elastase-1

Gene
N/A
Organism
Salmo salar (Atlantic salmon)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts upon elastin.1 Publication

Catalytic activityi

Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa.1 Publication

Cofactori

Ca2+1 PublicationNote: Binds 1 Ca2+ ion per subunit.1 Publication

Kineticsi

  1. KM=1.47 mM for Suc-AAA-pNA (at 22 degrees Celsius)1 Publication
  2. KM=1.34 mM for Suc-AAPL-pNA (at 22 degrees Celsius)1 Publication
  3. KM=0.82 mM for Suc-AAPV-pNA (at 22 degrees Celsius)1 Publication
  4. KM=0.83 mM for Suc-AAPA-pNA (at 22 degrees Celsius)1 Publication
  5. KM=0.15 mM for Suc-AAPI-pNA (at 22 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 8.1 at 22 degrees Celsius with Suc-AAA-pNA as the substrate.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei45Charge relay system1 Publication1
    Metal bindingi59Calcium1 Publication1
    Metal bindingi61Calcium; via carbonyl oxygen1
    Metal bindingi64Calcium; via carbonyl oxygen1
    Metal bindingi66Calcium1 Publication1
    Metal bindingi69Calcium1 Publication1
    Active sitei93Charge relay system1 Publication1
    Active sitei187Charge relay system1 Publication1

    GO - Molecular functioni

    Keywordsi

    Molecular functionHydrolase, Protease, Serine protease
    LigandCalcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Elastase-1 (EC:3.4.21.36)
    OrganismiSalmo salar (Atlantic salmon)
    Taxonomic identifieri8030 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeSalmo
    Proteomesi
    • UP000087266 Componenti: Genome assembly

    Subcellular locationi

    • Secreted 1 Publication

    GO - Cellular componenti

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002482611 – 236Elastase-1Add BLAST236

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi30 ↔ 46PROSITE-ProRule annotation1 Publication
    Disulfide bondi127 ↔ 193PROSITE-ProRule annotation1 Publication
    Disulfide bondi158 ↔ 174PROSITE-ProRule annotation1 Publication
    Disulfide bondi183 ↔ 213PROSITE-ProRule annotation1 Publication

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Tissue specificityi

    Pancreas.1 Publication

    Structurei

    Secondary structure

    1236
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi2 – 6Combined sources5
    Beta strandi15 – 22Combined sources8
    Beta strandi25 – 36Combined sources12
    Beta strandi39 – 42Combined sources4
    Helixi44 – 47Combined sources4
    Beta strandi53 – 58Combined sources6
    Beta strandi70 – 72Combined sources3
    Beta strandi74 – 79Combined sources6
    Helixi88 – 90Combined sources3
    Beta strandi95 – 101Combined sources7
    Beta strandi106 – 108Combined sources3
    Beta strandi127 – 132Combined sources6
    Beta strandi146 – 149Combined sources4
    Helixi155 – 158Combined sources4
    Turni161 – 164Combined sources4
    Helixi165 – 167Combined sources3
    Beta strandi172 – 175Combined sources4
    Beta strandi190 – 195Combined sources6
    Beta strandi198 – 207Combined sources10
    Beta strandi220 – 224Combined sources5
    Helixi225 – 228Combined sources4
    Helixi229 – 235Combined sources7

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1ELTX-ray1.61A1-236[»]
    ProteinModelPortaliQ7SIG3.
    SMRiQ7SIG3.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ7SIG3.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini1 – 236Peptidase S1PROSITE-ProRule annotationAdd BLAST236

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Elastase subfamily.PROSITE-ProRule annotation

    Phylogenomic databases

    HOVERGENiHBG013304.

    Family and domain databases

    CDDicd00190. Tryp_SPc. 1 hit.
    InterProiView protein in InterPro
    IPR009003. Peptidase_S1_PA.
    IPR001314. Peptidase_S1A.
    IPR001254. Trypsin_dom.
    IPR018114. TRYPSIN_HIS.
    IPR033116. TRYPSIN_SER.
    PfamiView protein in Pfam
    PF00089. Trypsin. 1 hit.
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiView protein in SMART
    SM00020. Tryp_SPc. 1 hit.
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiView protein in PROSITE
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q7SIG3-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    VVGGRVAQPN SWPWQISLQY KSGSSYYHTC GGSLIRQGWV MTAAHCVDSA
    60 70 80 90 100
    RTWRVVLGEH NLNTNEGKEQ IMTVNSVFIH SGWNSDDVAG GYDIALLRLN
    110 120 130 140 150
    TQASLNSAVQ LAALPPSNQI LPNNNPCYIT GWGKTSTGGP LSDSLKQAWL
    160 170 180 190 200
    PSVDHATCSS SGWWGSTVKT TMVCAGGGAN SGCNGDSGGP LNCQVNGSYY
    210 220 230
    VHGVTSFVSS SGCNASKKPT VFTRVSAYIS WMNGIM
    Length:236
    Mass (Da):25,015
    Last modified:December 15, 2003 - v1
    Checksum:i06CF7DB4E1397E97
    GO

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

    Entry informationi

    Entry nameiELA1_SALSA
    AccessioniPrimary (citable) accession number: Q7SIG3
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: December 15, 2003
    Last modified: June 7, 2017
    This is version 69 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families