Reviewed, UniProtKB/Swiss-Prot Q7SIG2 (CTR1_SOLIN)

Names and origin

Protein names	Recommended name:     Chymotrypsin-1     EC=3.4.21.1 Alternative name(s):     Chymotrypsin I     Soli C1
Organism	Solenopsis invicta (Red imported fire ant) (Solenopsis wagneri)
Taxonomic identifier	13686 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Vespoidea › Formicidae › Myrmicinae › Solenopsis

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa. Ref.1
Subcellular location	Secreted › extracellular space.
Developmental stage	Expressed in fourth instar larvae. Ref.1
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 222

222

Chymotrypsin-1

PRO_0000248259

Regions

Domain

1 – 221

221

Peptidase S1

Sites

Active site

41

1

Charge relay system Ref.2

Active site

87

1

Charge relay system Ref.2

Active site

178

1

Charge relay system Ref.2

Amino acid modifications

Disulfide bond

26 ↔ 42

Ref.2

Disulfide bond

151 ↔ 164

Ref.2

Disulfide bond

174 ↔ 198

Ref.2

Secondary structure

1

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222

Helix Strand Turn

Details...

Beta strand

15 – 20

6

Beta strand

23 – 30

8

Beta strand

32 – 38

7

Helix

40 – 43

4

Helix

49 – 51

3

Beta strand

52 – 57

6

Beta strand

66 – 75

10

Turn

81 – 84

4

Beta strand

89 – 95

7

Beta strand

118 – 124

7

Beta strand

139 – 146

8

Helix

148 – 154

7

Beta strand

155 – 157

3

Beta strand

162 – 165

4

Beta strand

181 – 184

4

Beta strand

187 – 194

8

Turn

197 – 199

3

Beta strand

200 – 202

3

Beta strand

204 – 208

5

Helix

209 – 212

4

Helix

213 – 218

6

Sequences

	Sequence		Length	Mass (Da)
	Q7SIG2-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 862770BF2A44E652	FASTA	222	24,012
	10 20 30 40 50 60 IVGGKDAPVG KYPYQVSLRL SGSHRCGASI LDNNNVLTAA HCVDGLSNLN RLKVHVGTNY 70 80 90 100 110 120 LSESGDVYDV EDAVVNKNYD DFLLRNDVAL VHLTNPIKFN DLVQPIKLST NDEDLESNPC 130 140 150 160 170 180 TLTGWGSTRL GGNTPNALQE IELIVHPQKQ CERDQWRVID SHICTLTKRG EGACHGDSGG 190 200 210 220 PLVANGAQIG IVSFGSPCAL GEPDVYTRVS SFVSWINANL KK

References

[1]	"Proteolytic enzymes from larvae of the fire ant, Solenopsis invicta. Isolation and characterization of four serine endopeptidases." Whitworth S.T., Blum M.S., Travis J. J. Biol. Chem. 273:14430-14434(1998) [PubMed: 9603955] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-22, CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE. Tissue: Larva.
[2]	"The structure of an insect chymotrypsin." Botos I., Meyer E., Nguyen M., Swanson S.M., Koomen J.M., Russell D.H., Meyer E.F. J. Mol. Biol. 298:895-901(2000) [PubMed: 10801356] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) IN COMPLEX WITH INHIBITOR, DISULFIDE BONDS. Tissue: Larva.

Cross-references

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EQ9	X-ray	1.70	A/B	1-222	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

3.4.21.1. 274954.

Family and domain databases

InterPro

IPR018114. Peptidase_S1/S6_AS.
IPR001254. Peptidase_S1_S6.
IPR001314. Peptidase_S1A.
[Graphical view]

Pfam

PF00089. Trypsin. 1 hit.
[Graphical view]

PRINTS

PR00722. CHYMOTRYPSIN.

SMART

SM00020. Tryp_SPc. 1 hit.
[Graphical view]

PROSITE

PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CTR1_SOLIN

Accession

Primary (citable) accession number: Q7SIG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	December 15, 2003
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)