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Protein

Arsenite oxidase subunit AioA

Gene

aioA

Organism
Alcaligenes faecalis
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the detoxification of arsenic. Oxidizes As(III)O33- (arsenite) to the somewhat less toxic As(V)O43- (arsenate).

Catalytic activityi

Arsenite + H2O + 2 oxidized azurin = arsenate + 2 reduced azurin + 2 H+.

Cofactori

Protein has several cofactor binding sites:
  • [3Fe-4S] cluster1 PublicationNote: Binds 1 [3Fe-4S] cluster per subunit.1 Publication
  • Mo-bis(molybdopterin guanine dinucleotide)1 PublicationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi22 – 221Iron-sulfur (3Fe-4S)
Metal bindingi25 – 251Iron-sulfur (3Fe-4S)
Metal bindingi29 – 291Iron-sulfur (3Fe-4S)
Sitei100 – 1001Involved in charge transferCurated
Binding sitei196 – 1961Substrate
Binding sitei204 – 2041Substrate
Binding sitei420 – 4201Substrate
Binding sitei424 – 4241Substrate

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

3Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-10727.
BRENDAi1.20.9.1. 232.

Protein family/group databases

TCDBi5.A.3.6.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Arsenite oxidase subunit AioA (EC:1.20.9.1)
Short name:
AOI
Alternative name(s):
Arsenite oxidase Mo-pterin subunit
Gene namesi
Name:aioA
Synonyms:aoxB, asoA
OrganismiAlcaligenes faecalis
Taxonomic identifieri511 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeAlcaligenes

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved2 Publications
Chaini2 – 826825Arsenite oxidase subunit AioAPRO_0000063240Add
BLAST

Interactioni

Subunit structurei

Heterodimer consisting of a large and a small subunit.1 Publication

Structurei

Secondary structure

1
826
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi16 – 216Combined sources
Beta strandi23 – 253Combined sources
Beta strandi30 – 378Combined sources
Helixi46 – 483Combined sources
Helixi68 – 703Combined sources
Beta strandi71 – 755Combined sources
Beta strandi81 – 888Combined sources
Turni93 – 975Combined sources
Helixi103 – 1097Combined sources
Beta strandi113 – 1153Combined sources
Turni116 – 1194Combined sources
Beta strandi125 – 1273Combined sources
Beta strandi129 – 1346Combined sources
Helixi137 – 15418Combined sources
Helixi157 – 1593Combined sources
Beta strandi160 – 1645Combined sources
Helixi174 – 18512Combined sources
Turni186 – 1883Combined sources
Beta strandi193 – 1953Combined sources
Beta strandi198 – 2014Combined sources
Helixi206 – 2105Combined sources
Helixi220 – 2256Combined sources
Beta strandi227 – 2337Combined sources
Helixi236 – 2394Combined sources
Helixi241 – 2466Combined sources
Helixi248 – 2525Combined sources
Turni253 – 2564Combined sources
Helixi257 – 2637Combined sources
Beta strandi273 – 2775Combined sources
Helixi283 – 29210Combined sources
Helixi294 – 2963Combined sources
Beta strandi297 – 3004Combined sources
Helixi307 – 32115Combined sources
Helixi326 – 3327Combined sources
Helixi336 – 3427Combined sources
Helixi347 – 3548Combined sources
Helixi358 – 36912Combined sources
Beta strandi380 – 3845Combined sources
Helixi386 – 3894Combined sources
Helixi394 – 40714Combined sources
Beta strandi411 – 4133Combined sources
Beta strandi418 – 4203Combined sources
Helixi441 – 4466Combined sources
Beta strandi451 – 4577Combined sources
Helixi460 – 4634Combined sources
Helixi467 – 48620Combined sources
Turni488 – 4903Combined sources
Helixi493 – 50412Combined sources
Turni505 – 5073Combined sources
Beta strandi510 – 5178Combined sources
Helixi520 – 5234Combined sources
Beta strandi526 – 5316Combined sources
Beta strandi539 – 5424Combined sources
Beta strandi547 – 5515Combined sources
Helixi564 – 58118Combined sources
Helixi585 – 5906Combined sources
Helixi599 – 6057Combined sources
Helixi607 – 6093Combined sources
Helixi623 – 6286Combined sources
Helixi631 – 6377Combined sources
Helixi638 – 6403Combined sources
Beta strandi642 – 6454Combined sources
Beta strandi648 – 6503Combined sources
Turni651 – 6533Combined sources
Beta strandi654 – 6563Combined sources
Beta strandi659 – 6613Combined sources
Beta strandi672 – 6754Combined sources
Helixi686 – 6949Combined sources
Beta strandi696 – 7027Combined sources
Helixi714 – 7163Combined sources
Helixi718 – 7236Combined sources
Beta strandi728 – 7314Combined sources
Helixi733 – 7386Combined sources
Beta strandi745 – 7506Combined sources
Beta strandi755 – 7628Combined sources
Beta strandi770 – 7745Combined sources
Beta strandi778 – 7803Combined sources
Helixi782 – 7854Combined sources
Beta strandi802 – 8109Combined sources
Helixi812 – 8176Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G8JX-ray2.03A/C4-825[»]
1G8KX-ray1.64A/C/E/G4-825[»]
ProteinModelPortaliQ7SIF4.
SMRiQ7SIF4. Positions 5-826.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7SIF4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK08356.

Family and domain databases

InterProiIPR014066. Arsenite_oxidase_lsu.
IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
[Graphical view]
PfamiPF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR02693. arsenite_ox_L. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7SIF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRPNDRITL PPANAQRTNM TCHFCIVGCG YHVYKWPELQ EGGRAPEQNA
60 70 80 90 100
LGLDFRKQLP PLAVTLTPAM TNVVTEHNGR RYNIMVVPDK ACVVNSGLSS
110 120 130 140 150
TRGGKMASYM YTPTGDGKQR LKAPRLYAAD QWVDTTWDHA MALYAGLIKK
160 170 180 190 200
TLDKDGPQGV FFSCFDHGGA GGGFENTWGT GKLMFSAIQT PMVRIHNRPA
210 220 230 240 250
YNSECHATRE MGIGELNNAY EDAQLADVIW SIGNNPYESQ TNYFLNHWLP
260 270 280 290 300
NLQGATTSKK KERFPNENFP QARIIFVDPR ETPSVAIARH VAGNDRVLHL
310 320 330 340 350
AIEPGTDTAL FNGLFTYVVE QGWIDKPFIE AHTKGFDDAV KTNRLSLDEC
360 370 380 390 400
SNITGVPVDM LKRAAEWSYK PKASGQAPRT MHAYEKGIIW GNDNYVIQSA
410 420 430 440 450
LLDLVIATHN VGRRGTGCVR MGGHQEGYTR PPYPGDKKIY IDQELIKGKG
460 470 480 490 500
RIMTWWGCNN FQTSNNAQAL REAILQRSAI VKQAMQKARG ATTEEMVDVI
510 520 530 540 550
YEATQNGGLF VTSINLYPTK LAEAAHLMLP AAHPGEMNLT SMNGERRIRL
560 570 580 590 600
SEKFMDPPGT AMADCLIAAR IANALRDMYQ KDGKAEMAAQ FEGFDWKTEE
610 620 630 640 650
DAFNDGFRRA GQPGAPAIDS QGGSTGHLVT YDRLRKSGNN GVQLPVVSWD
660 670 680 690 700
ESKGLVGTEM LYTEGKFDTD DGKAHFKPAP WNGLPATVQQ QKDKYRFWLN
710 720 730 740 750
NGRNNEVWQT AYHDQYNSLM QERYPMAYIE MNPDDCKQLD VTGGDIVEVY
760 770 780 790 800
NDFGSTFAMV YPVAEIKRGQ TFMLFGYVNG IQGDVTTDWT DRNIIPYYKG
810 820
TWGDIRKVGS MEEFKRTVSF KSRRFA
Length:826
Mass (Da):92,395
Last modified:January 25, 2012 - v2
Checksum:i56AFA94C70E82575
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 32SR → GC AA sequence (PubMed:11250197).Curated
Sequence conflicti2 – 32SR → GC AA sequence (PubMed:1331097).Curated
Sequence conflicti40 – 401Q → E AA sequence (PubMed:11250197).Curated
Sequence conflicti64 – 641V → S AA sequence (PubMed:11250197).Curated
Sequence conflicti78 – 836NGRRYN → DGARYD AA sequence (PubMed:11250197).Curated
Sequence conflicti119 – 1191Q → E AA sequence (PubMed:11250197).Curated
Sequence conflicti122 – 1221K → S AA sequence (PubMed:11250197).Curated
Sequence conflicti131 – 1311Q → E AA sequence (PubMed:11250197).Curated
Sequence conflicti154 – 1541K → S AA sequence (PubMed:11250197).Curated
Sequence conflicti793 – 7931N → D AA sequence (PubMed:11250197).Curated
Sequence conflicti812 – 8121E → S AA sequence (PubMed:11250197).Curated
Sequence conflicti826 – 8261A → G AA sequence (PubMed:11250197).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY297781 Genomic DNA. Translation: AAQ19838.1.
PIRiB45138.

Genome annotation databases

KEGGiag:AAQ19838.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY297781 Genomic DNA. Translation: AAQ19838.1.
PIRiB45138.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G8JX-ray2.03A/C4-825[»]
1G8KX-ray1.64A/C/E/G4-825[»]
ProteinModelPortaliQ7SIF4.
SMRiQ7SIF4. Positions 5-826.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi5.A.3.6.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAQ19838.

Phylogenomic databases

KOiK08356.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-10727.
BRENDAi1.20.9.1. 232.

Miscellaneous databases

EvolutionaryTraceiQ7SIF4.

Family and domain databases

InterProiIPR014066. Arsenite_oxidase_lsu.
IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
[Graphical view]
PfamiPF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR02693. arsenite_ox_L. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genes for arsenite oxidation from Alcaligenes faecalis."
    Silver S., Phung L.T., Malo B.J.
    Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CCUG 2071 / LMG 3368 / NCIB 8687.
  2. "Crystal structure of the 100 kDa arsenite oxidase from Alcaligenes faecalis in two crystal forms at 1.64 A and 2.03 A."
    Ellis P.J., Conrads T., Hille R., Kuhn P.
    Structure 9:125-132(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-826, X-RAY CRYSTALLOGRAPHY (1.64 ANGSTROMS) IN COMPLEX WITH MO-BIS-MGD AND IRON-SULFUR CLUSTER, COFACTOR.
    Strain: CCUG 2071 / LMG 3368 / NCIB 8687.
  3. "The purification and characterization of arsenite oxidase from Alcaligenes faecalis, a molybdenum-containing hydroxylase."
    Anderson G.L., Williams J., Hille R.
    J. Biol. Chem. 267:23674-23682(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-18.
    Strain: CCUG 2071 / LMG 3368 / NCIB 8687.
  4. "Unified nomenclature for genes involved in prokaryotic aerobic arsenite oxidation."
    Lett M.C., Muller D., Lievremont D., Silver S., Santini J.
    J. Bacteriol. 194:207-208(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.

Entry informationi

Entry nameiAIOA_ALCFA
AccessioniPrimary (citable) accession number: Q7SIF4
Secondary accession number(s): Q6WB60, Q9R5F9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: January 25, 2012
Last modified: May 11, 2016
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.