Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Arsenite oxidase subunit AioA

Gene

aioA

Organism
Alcaligenes faecalis
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the detoxification of arsenic. Oxidizes As(III)O33- (arsenite) to the somewhat less toxic As(V)O43- (arsenate).

Catalytic activityi

Arsenite + H2O + 2 oxidized azurin = arsenate + 2 reduced azurin + 2 H+.

Cofactori

Protein has several cofactor binding sites:
  • [3Fe-4S] cluster1 PublicationNote: Binds 1 [3Fe-4S] cluster per subunit.1 Publication
  • Mo-bis(molybdopterin guanine dinucleotide)1 PublicationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi22Iron-sulfur (3Fe-4S)1
Metal bindingi25Iron-sulfur (3Fe-4S)1
Metal bindingi29Iron-sulfur (3Fe-4S)1
Sitei100Involved in charge transferCurated1
Binding sitei196Substrate1
Binding sitei204Substrate1
Binding sitei420Substrate1
Binding sitei424Substrate1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

3Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-10727.
BRENDAi1.20.9.1. 232.

Protein family/group databases

TCDBi5.A.3.6.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Arsenite oxidase subunit AioA (EC:1.20.9.1)
Short name:
AOI
Alternative name(s):
Arsenite oxidase Mo-pterin subunit
Gene namesi
Name:aioA
Synonyms:aoxB, asoA
OrganismiAlcaligenes faecalis
Taxonomic identifieri511 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeAlcaligenes

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00000632402 – 826Arsenite oxidase subunit AioAAdd BLAST825

Interactioni

Subunit structurei

Heterodimer consisting of a large and a small subunit.1 Publication

Structurei

Secondary structure

1826
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi16 – 21Combined sources6
Beta strandi23 – 25Combined sources3
Beta strandi30 – 37Combined sources8
Helixi46 – 48Combined sources3
Helixi68 – 70Combined sources3
Beta strandi71 – 75Combined sources5
Beta strandi81 – 88Combined sources8
Turni93 – 97Combined sources5
Helixi103 – 109Combined sources7
Beta strandi113 – 115Combined sources3
Turni116 – 119Combined sources4
Beta strandi125 – 127Combined sources3
Beta strandi129 – 134Combined sources6
Helixi137 – 154Combined sources18
Helixi157 – 159Combined sources3
Beta strandi160 – 164Combined sources5
Helixi174 – 185Combined sources12
Turni186 – 188Combined sources3
Beta strandi193 – 195Combined sources3
Beta strandi198 – 201Combined sources4
Helixi206 – 210Combined sources5
Helixi220 – 225Combined sources6
Beta strandi227 – 233Combined sources7
Helixi236 – 239Combined sources4
Helixi241 – 246Combined sources6
Helixi248 – 252Combined sources5
Turni253 – 256Combined sources4
Helixi257 – 263Combined sources7
Beta strandi273 – 277Combined sources5
Helixi283 – 292Combined sources10
Helixi294 – 296Combined sources3
Beta strandi297 – 300Combined sources4
Helixi307 – 321Combined sources15
Helixi326 – 332Combined sources7
Helixi336 – 342Combined sources7
Helixi347 – 354Combined sources8
Helixi358 – 369Combined sources12
Beta strandi380 – 384Combined sources5
Helixi386 – 389Combined sources4
Helixi394 – 407Combined sources14
Beta strandi411 – 413Combined sources3
Beta strandi418 – 420Combined sources3
Helixi441 – 446Combined sources6
Beta strandi451 – 457Combined sources7
Helixi460 – 463Combined sources4
Helixi467 – 486Combined sources20
Turni488 – 490Combined sources3
Helixi493 – 504Combined sources12
Turni505 – 507Combined sources3
Beta strandi510 – 517Combined sources8
Helixi520 – 523Combined sources4
Beta strandi526 – 531Combined sources6
Beta strandi539 – 542Combined sources4
Beta strandi547 – 551Combined sources5
Helixi564 – 581Combined sources18
Helixi585 – 590Combined sources6
Helixi599 – 605Combined sources7
Helixi607 – 609Combined sources3
Helixi623 – 628Combined sources6
Helixi631 – 637Combined sources7
Helixi638 – 640Combined sources3
Beta strandi642 – 645Combined sources4
Beta strandi648 – 650Combined sources3
Turni651 – 653Combined sources3
Beta strandi654 – 656Combined sources3
Beta strandi659 – 661Combined sources3
Beta strandi672 – 675Combined sources4
Helixi686 – 694Combined sources9
Beta strandi696 – 702Combined sources7
Helixi714 – 716Combined sources3
Helixi718 – 723Combined sources6
Beta strandi728 – 731Combined sources4
Helixi733 – 738Combined sources6
Beta strandi745 – 750Combined sources6
Beta strandi755 – 762Combined sources8
Beta strandi770 – 774Combined sources5
Beta strandi778 – 780Combined sources3
Helixi782 – 785Combined sources4
Beta strandi802 – 810Combined sources9
Helixi812 – 817Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G8JX-ray2.03A/C4-825[»]
1G8KX-ray1.64A/C/E/G4-825[»]
ProteinModelPortaliQ7SIF4.
SMRiQ7SIF4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7SIF4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK08356.

Family and domain databases

InterProiIPR014066. Arsenite_oxidase_lsu.
IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
[Graphical view]
PfamiPF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR02693. arsenite_ox_L. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7SIF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRPNDRITL PPANAQRTNM TCHFCIVGCG YHVYKWPELQ EGGRAPEQNA
60 70 80 90 100
LGLDFRKQLP PLAVTLTPAM TNVVTEHNGR RYNIMVVPDK ACVVNSGLSS
110 120 130 140 150
TRGGKMASYM YTPTGDGKQR LKAPRLYAAD QWVDTTWDHA MALYAGLIKK
160 170 180 190 200
TLDKDGPQGV FFSCFDHGGA GGGFENTWGT GKLMFSAIQT PMVRIHNRPA
210 220 230 240 250
YNSECHATRE MGIGELNNAY EDAQLADVIW SIGNNPYESQ TNYFLNHWLP
260 270 280 290 300
NLQGATTSKK KERFPNENFP QARIIFVDPR ETPSVAIARH VAGNDRVLHL
310 320 330 340 350
AIEPGTDTAL FNGLFTYVVE QGWIDKPFIE AHTKGFDDAV KTNRLSLDEC
360 370 380 390 400
SNITGVPVDM LKRAAEWSYK PKASGQAPRT MHAYEKGIIW GNDNYVIQSA
410 420 430 440 450
LLDLVIATHN VGRRGTGCVR MGGHQEGYTR PPYPGDKKIY IDQELIKGKG
460 470 480 490 500
RIMTWWGCNN FQTSNNAQAL REAILQRSAI VKQAMQKARG ATTEEMVDVI
510 520 530 540 550
YEATQNGGLF VTSINLYPTK LAEAAHLMLP AAHPGEMNLT SMNGERRIRL
560 570 580 590 600
SEKFMDPPGT AMADCLIAAR IANALRDMYQ KDGKAEMAAQ FEGFDWKTEE
610 620 630 640 650
DAFNDGFRRA GQPGAPAIDS QGGSTGHLVT YDRLRKSGNN GVQLPVVSWD
660 670 680 690 700
ESKGLVGTEM LYTEGKFDTD DGKAHFKPAP WNGLPATVQQ QKDKYRFWLN
710 720 730 740 750
NGRNNEVWQT AYHDQYNSLM QERYPMAYIE MNPDDCKQLD VTGGDIVEVY
760 770 780 790 800
NDFGSTFAMV YPVAEIKRGQ TFMLFGYVNG IQGDVTTDWT DRNIIPYYKG
810 820
TWGDIRKVGS MEEFKRTVSF KSRRFA
Length:826
Mass (Da):92,395
Last modified:January 25, 2012 - v2
Checksum:i56AFA94C70E82575
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2 – 3SR → GC AA sequence (PubMed:11250197).Curated2
Sequence conflicti2 – 3SR → GC AA sequence (PubMed:1331097).Curated2
Sequence conflicti40Q → E AA sequence (PubMed:11250197).Curated1
Sequence conflicti64V → S AA sequence (PubMed:11250197).Curated1
Sequence conflicti78 – 83NGRRYN → DGARYD AA sequence (PubMed:11250197).Curated6
Sequence conflicti119Q → E AA sequence (PubMed:11250197).Curated1
Sequence conflicti122K → S AA sequence (PubMed:11250197).Curated1
Sequence conflicti131Q → E AA sequence (PubMed:11250197).Curated1
Sequence conflicti154K → S AA sequence (PubMed:11250197).Curated1
Sequence conflicti793N → D AA sequence (PubMed:11250197).Curated1
Sequence conflicti812E → S AA sequence (PubMed:11250197).Curated1
Sequence conflicti826A → G AA sequence (PubMed:11250197).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY297781 Genomic DNA. Translation: AAQ19838.1.
PIRiB45138.

Genome annotation databases

KEGGiag:AAQ19838.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY297781 Genomic DNA. Translation: AAQ19838.1.
PIRiB45138.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G8JX-ray2.03A/C4-825[»]
1G8KX-ray1.64A/C/E/G4-825[»]
ProteinModelPortaliQ7SIF4.
SMRiQ7SIF4.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi5.A.3.6.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAQ19838.

Phylogenomic databases

KOiK08356.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-10727.
BRENDAi1.20.9.1. 232.

Miscellaneous databases

EvolutionaryTraceiQ7SIF4.

Family and domain databases

InterProiIPR014066. Arsenite_oxidase_lsu.
IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
[Graphical view]
PfamiPF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR02693. arsenite_ox_L. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAIOA_ALCFA
AccessioniPrimary (citable) accession number: Q7SIF4
Secondary accession number(s): Q6WB60, Q9R5F9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: January 25, 2012
Last modified: November 2, 2016
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.