Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Arsenite oxidase subunit AioB

Gene

aioB

Organism
Alcaligenes faecalis
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the detoxification of arsenic. Oxidizes As(III)O33- (arsenite) to the somewhat less toxic As(V)O43- (arsenate).

Catalytic activityi

Arsenite + H2O + 2 oxidized azurin = arsenate + 2 reduced azurin + 2 H+.

Cofactori

[2Fe-2S] clusterNote: Binds 1 [2Fe-2S] cluster per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi102 – 1021Iron-sulfur (2Fe-2S)
Metal bindingi104 – 1041Iron-sulfur (2Fe-2S); via pros nitrogen
Metal bindingi120 – 1201Iron-sulfur (2Fe-2S)
Metal bindingi123 – 1231Iron-sulfur (2Fe-2S); via pros nitrogen

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-10728.
BRENDAi1.20.9.1. 232.

Protein family/group databases

TCDBi5.A.3.6.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Arsenite oxidase subunit AioB (EC:1.20.9.1)
Short name:
AOII
Alternative name(s):
Arsenite oxidase Rieske subunit
Gene namesi
Name:aioB
Synonyms:aoxA, asoB
OrganismiAlcaligenes faecalis
Taxonomic identifieri511 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeAlcaligenes

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Tat-type signalPROSITE-ProRule annotationAdd
BLAST
Chaini33 – 175143Arsenite oxidase subunit AioBPRO_0000127798Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi107 ↔ 122

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Heterodimer consisting of a large and a small subunit.

Structurei

Secondary structure

1
175
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi51 – 555Combined sources
Helixi56 – 583Combined sources
Beta strandi65 – 684Combined sources
Beta strandi74 – 807Combined sources
Turni90 – 934Combined sources
Beta strandi95 – 995Combined sources
Turni103 – 1053Combined sources
Beta strandi110 – 1123Combined sources
Turni113 – 1164Combined sources
Beta strandi117 – 1193Combined sources
Turni121 – 1233Combined sources
Beta strandi126 – 1283Combined sources
Helixi129 – 1313Combined sources
Beta strandi135 – 1395Combined sources
Beta strandi146 – 1516Combined sources
Turni153 – 1553Combined sources
Beta strandi157 – 1659Combined sources
Beta strandi171 – 1733Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G8JX-ray2.03B/D43-175[»]
1G8KX-ray1.64B/D/F/H43-175[»]
ProteinModelPortaliQ7SIF3.
SMRiQ7SIF3. Positions 43-175.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7SIF3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini62 – 15897RieskePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the AOX family.Curated
Contains 1 Rieske domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

KOiK08355.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
InterProiIPR014067. Arsenite_oxidase_ssu.
IPR017941. Rieske_2Fe-2S.
IPR005805. Rieske_Fe-S_prot_C.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
TIGRFAMsiTIGR02694. arsenite_ox_S. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7SIF3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDTINLTRR GFLKVSGSGV AVAATLSPIA SANAQKAPAD AGRTTLQYPA
60 70 80 90 100
TQVSVAKNLK ANEPVSFTYP DTSSPCVAVK LGSPVPGGVG PNNDIVAYSV
110 120 130 140 150
LCTHMGCPTS YDKSSKTFKC PCHFTEFDAE KAGQMICGQA TENLPRVLLR
160 170
YDEASDALTA VGVDGLIYGR QANVI
Length:175
Mass (Da):18,275
Last modified:January 25, 2012 - v2
Checksum:i9284053031A809E7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti45 – 451T → R AA sequence (PubMed:1331097).Curated
Sequence conflicti47 – 471Q → A AA sequence (PubMed:11250197).Curated
Sequence conflicti51 – 511T → C AA sequence (PubMed:1331097).Curated
Sequence conflicti52 – 521Q → A AA sequence (PubMed:11250197).Curated
Sequence conflicti56 – 561A → V AA sequence (PubMed:1331097).Curated
Sequence conflicti60 – 601K → A AA sequence (PubMed:11250197).Curated
Sequence conflicti65 – 651V → S AA sequence (PubMed:11250197).Curated
Sequence conflicti83 – 831S → A AA sequence (PubMed:11250197).Curated
Sequence conflicti92 – 932NN → DD AA sequence (PubMed:11250197).Curated
Sequence conflicti113 – 1131K → S AA sequence (PubMed:11250197).Curated
Sequence conflicti119 – 1191K → S AA sequence (PubMed:11250197).Curated
Sequence conflicti139 – 1391Q → E AA sequence (PubMed:11250197).Curated
Sequence conflicti142 – 1432EN → AD AA sequence (PubMed:11250197).Curated
Sequence conflicti153 – 1531E → A AA sequence (PubMed:11250197).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY297781 Genomic DNA. Translation: AAQ19839.1.
PIRiA45138.

Genome annotation databases

KEGGiag:AAQ19839.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY297781 Genomic DNA. Translation: AAQ19839.1.
PIRiA45138.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G8JX-ray2.03B/D43-175[»]
1G8KX-ray1.64B/D/F/H43-175[»]
ProteinModelPortaliQ7SIF3.
SMRiQ7SIF3. Positions 43-175.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi5.A.3.6.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAQ19839.

Phylogenomic databases

KOiK08355.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-10728.
BRENDAi1.20.9.1. 232.

Miscellaneous databases

EvolutionaryTraceiQ7SIF3.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
InterProiIPR014067. Arsenite_oxidase_ssu.
IPR017941. Rieske_2Fe-2S.
IPR005805. Rieske_Fe-S_prot_C.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
TIGRFAMsiTIGR02694. arsenite_ox_S. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAIOB_ALCFA
AccessioniPrimary (citable) accession number: Q7SIF3
Secondary accession number(s): Q6WB59, Q9R5G0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: January 25, 2012
Last modified: May 11, 2016
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.