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Protein

Arsenite oxidase subunit AioB

Gene

aioB

Organism
Alcaligenes faecalis
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the detoxification of arsenic. Oxidizes As(III)O33- (arsenite) to the somewhat less toxic As(V)O43- (arsenate).

Catalytic activityi

Arsenite + H2O + 2 oxidized azurin = arsenate + 2 reduced azurin + 2 H+.

Cofactori

[2Fe-2S] clusterNote: Binds 1 [2Fe-2S] cluster per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi102Iron-sulfur (2Fe-2S)1
Metal bindingi104Iron-sulfur (2Fe-2S); via pros nitrogen1
Metal bindingi120Iron-sulfur (2Fe-2S)1
Metal bindingi123Iron-sulfur (2Fe-2S); via pros nitrogen1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-10728.
BRENDAi1.20.9.1. 232.

Protein family/group databases

TCDBi5.A.3.6.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Arsenite oxidase subunit AioB (EC:1.20.9.1)
Short name:
AOII
Alternative name(s):
Arsenite oxidase Rieske subunit
Gene namesi
Name:aioB
Synonyms:aoxA, asoB
OrganismiAlcaligenes faecalis
Taxonomic identifieri511 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeAlcaligenes

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Tat-type signalPROSITE-ProRule annotationAdd BLAST32
ChainiPRO_000012779833 – 175Arsenite oxidase subunit AioBAdd BLAST143

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi107 ↔ 122

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Heterodimer consisting of a large and a small subunit.

Structurei

Secondary structure

1175
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi51 – 55Combined sources5
Helixi56 – 58Combined sources3
Beta strandi65 – 68Combined sources4
Beta strandi74 – 80Combined sources7
Turni90 – 93Combined sources4
Beta strandi95 – 99Combined sources5
Turni103 – 105Combined sources3
Beta strandi110 – 112Combined sources3
Turni113 – 116Combined sources4
Beta strandi117 – 119Combined sources3
Turni121 – 123Combined sources3
Beta strandi126 – 128Combined sources3
Helixi129 – 131Combined sources3
Beta strandi135 – 139Combined sources5
Beta strandi146 – 151Combined sources6
Turni153 – 155Combined sources3
Beta strandi157 – 165Combined sources9
Beta strandi171 – 173Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G8JX-ray2.03B/D43-175[»]
1G8KX-ray1.64B/D/F/H43-175[»]
ProteinModelPortaliQ7SIF3.
SMRiQ7SIF3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7SIF3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini62 – 158RieskePROSITE-ProRule annotationAdd BLAST97

Sequence similaritiesi

Belongs to the AOX family.Curated
Contains 1 Rieske domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

KOiK08355.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
InterProiIPR014067. Arsenite_oxidase_ssu.
IPR017941. Rieske_2Fe-2S.
IPR005805. Rieske_Fe-S_prot_C.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
TIGRFAMsiTIGR02694. arsenite_ox_S. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7SIF3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDTINLTRR GFLKVSGSGV AVAATLSPIA SANAQKAPAD AGRTTLQYPA
60 70 80 90 100
TQVSVAKNLK ANEPVSFTYP DTSSPCVAVK LGSPVPGGVG PNNDIVAYSV
110 120 130 140 150
LCTHMGCPTS YDKSSKTFKC PCHFTEFDAE KAGQMICGQA TENLPRVLLR
160 170
YDEASDALTA VGVDGLIYGR QANVI
Length:175
Mass (Da):18,275
Last modified:January 25, 2012 - v2
Checksum:i9284053031A809E7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45T → R AA sequence (PubMed:1331097).Curated1
Sequence conflicti47Q → A AA sequence (PubMed:11250197).Curated1
Sequence conflicti51T → C AA sequence (PubMed:1331097).Curated1
Sequence conflicti52Q → A AA sequence (PubMed:11250197).Curated1
Sequence conflicti56A → V AA sequence (PubMed:1331097).Curated1
Sequence conflicti60K → A AA sequence (PubMed:11250197).Curated1
Sequence conflicti65V → S AA sequence (PubMed:11250197).Curated1
Sequence conflicti83S → A AA sequence (PubMed:11250197).Curated1
Sequence conflicti92 – 93NN → DD AA sequence (PubMed:11250197).Curated2
Sequence conflicti113K → S AA sequence (PubMed:11250197).Curated1
Sequence conflicti119K → S AA sequence (PubMed:11250197).Curated1
Sequence conflicti139Q → E AA sequence (PubMed:11250197).Curated1
Sequence conflicti142 – 143EN → AD AA sequence (PubMed:11250197).Curated2
Sequence conflicti153E → A AA sequence (PubMed:11250197).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY297781 Genomic DNA. Translation: AAQ19839.1.
PIRiA45138.

Genome annotation databases

KEGGiag:AAQ19839.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY297781 Genomic DNA. Translation: AAQ19839.1.
PIRiA45138.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G8JX-ray2.03B/D43-175[»]
1G8KX-ray1.64B/D/F/H43-175[»]
ProteinModelPortaliQ7SIF3.
SMRiQ7SIF3.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi5.A.3.6.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAQ19839.

Phylogenomic databases

KOiK08355.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-10728.
BRENDAi1.20.9.1. 232.

Miscellaneous databases

EvolutionaryTraceiQ7SIF3.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
InterProiIPR014067. Arsenite_oxidase_ssu.
IPR017941. Rieske_2Fe-2S.
IPR005805. Rieske_Fe-S_prot_C.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
TIGRFAMsiTIGR02694. arsenite_ox_S. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAIOB_ALCFA
AccessioniPrimary (citable) accession number: Q7SIF3
Secondary accession number(s): Q6WB59, Q9R5G0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: January 25, 2012
Last modified: November 2, 2016
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.