Q7SIE3 (Q7SIE3_BACAG) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Recommended name: Endo-1,4-beta-xylanase RuleBase RU004392 EC=3.2.1.8 RuleBase RU004392
Organism	Bacillus agaradhaerens (Bacillus agaradherans) PDB 1H4G
Taxonomic identifier	76935 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans. RuleBase RU004392
Pathway	Glycan degradation; xylan degradation. RuleBase RU004392
Sequence similarities	Belongs to the glycosyl hydrolase 11 (cellulase G) family.

Ontologies

Keywords
Biological process	Xylan degradation RuleBase RU003433 RuleBase RU004392
Molecular function	Glycosidase RuleBase RU003433 RuleBase RU004392 Hydrolase
Technical term	3D-structure PDB 1H4G PDB 1QH7 PDB 1QH6 PDB 2F6B PDB 2NQY
Gene Ontology (GO)
Biological_process	xylan catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	endo-1,4-beta-xylanase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	94	1	Nucleophile PDB 1H4G
	Active site	184	1	Proton donor/acceptor PDB 1H4G

Sequences

	Sequence		Length	Mass (Da)
	Q7SIE3 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: C498EF0D0A085D0B	FASTA	207	23,152
	10 20 30 40 50 60 QIVTDNSIGN HDGYDYEFWK DSGGSGTMIL NHGGTFSAQW NNVNNILFRK GKKFNETQTH 70 80 90 100 110 120 QQVGNMSINY GANFQPNGNA YLCVYGWTVD PLVEYYIVDS WGNWRPPGAT PKGTITVDGG 130 140 150 160 170 180 TYDIYETLRV NQPSIKGIAT FKQYWSVRRS KRTSGTISVS NHFRAWENLG MNMGKMYEVA 190 200 LTVEGYQSSG SANVYSNTLR INGNPLS

References

[1]	"Catalysis and specificity in enzymatic glycoside hydrolysis: a 2,5B conformation for the glycosyl-enzyme intermediate revealed by the structure of the Bacillus agaradhaerens family 11 xylanase." Sabini E., Sulzenbacher G., Dauter M., Dauter Z., Jorgensen P.L., Schulein M., Dupont C., Davies G.J., Wilson K.S. Chem. Biol. 6:483-492(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.10 ANGSTROMS) OF 2-207, ACTIVE SITE.
[2]	"Structural and active site modification studies implicate Glu, Trp and Arg in the activity of xylanase from alkalophilic Bacillus sp. (NCL 87-6-10)." Balakrishnan H., Satyanarayana L., Gaikward S., Suresh C.G. Submitted (NOV-2005) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) OF 2-206.
[3]	"Crystal structure of alkaline thermophlic xylanase from Bacillus sp. (NCL 86-6-10) with complex xylotriose: Xylotriose cleaved to xylobiose and xylose." Satyanarayana L., Gaikwad S.M., Balakrishnan H., Suresh C.G. Submitted (NOV-2006) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 2-206.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1H4G	X-ray	1.10	A/B	2-207	[»]
1QH6	X-ray	2.00	A/B	2-207	[»]
1QH7	X-ray	1.78	A/B	2-207	[»]
2F6B	X-ray	2.80	A/B	2-206	[»]
2NQY	X-ray	2.40	A/B	2-206	[»]

ProteinModelPortal

Q7SIE3.

SMR

Q7SIE3. Positions 1-207.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Enzyme and pathway databases

UniPathway

UPA00114.

Family and domain databases

Gene3D

2.60.120.180. 1 hit.

InterPro

IPR008985. ConA-like_lec_gl_sf.
IPR001137. Glyco_hydro_11.
IPR013319. Glyco_hydro_11/12.
IPR018208. Glyco_hydro_11_AS.
[Graphical view]

Pfam

PF00457. Glyco_hydro_11. 1 hit.
[Graphical view]

PRINTS

PR00911. GLHYDRLASE11.

SUPFAM

SSF49899. ConA_like_lec_gl. 1 hit.

PROSITE

PS00776. GLYCOSYL_HYDROL_F11_1. 1 hit.
PS00777. GLYCOSYL_HYDROL_F11_2. 1 hit.
[Graphical view]

ProtoNet

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Other

EvolutionaryTrace

Q7SIE3.

Entry information

Entry name

Q7SIE3_BACAG

Accession

Primary (citable) accession number: Q7SIE3

Entry history

Integrated into UniProtKB/TrEMBL:	December 15, 2003
Last sequence update:	December 15, 2003
Last modified:	April 3, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)