ID   Q7SIE1_LEVBR            Unreviewed;       754 AA.
AC   Q7SIE1;
DT   15-DEC-2003, integrated into UniProtKB/TrEMBL.
DT   15-DEC-2003, sequence version 1.
DT   27-MAR-2024, entry version 85.
DE   SubName: Full=Hydrolase {ECO:0000313|PDB:1H54};
OS   Levilactobacillus brevis (Lactobacillus brevis).
OC   Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Levilactobacillus.
OX   NCBI_TaxID=1580 {ECO:0000313|PDB:1H54};
RN   [1] {ECO:0000313|PDB:1H54, ECO:0007829|PDB:1H54}
RP   X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS), AND ACTIVE SITE.
RX   PubMed=11587643; DOI=10.1016/S0969-2126(01)00626-8;
RA   Egloff M.P., Uppenberg J., Haalck L., van Tilbeurgh H.;
RT   "Crystal structure of maltose phosphorylase from Lactobacillus brevis:
RT   unexpected evolutionary relationship with glucoamylases.";
RL   Structure 9:689-697(2001).
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 65 family.
CC       {ECO:0000256|ARBA:ARBA00006768}.
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DR   PDB; 1H54; X-ray; 2.15 A; A/B=1-754.
DR   PDBsum; 1H54; -.
DR   AlphaFoldDB; Q7SIE1; -.
DR   SMR; Q7SIE1; -.
DR   CAZy; GH65; Glycoside Hydrolase Family 65.
DR   BioCyc; MetaCyc:MONOMER-13019; -.
DR   EvolutionaryTrace; Q7SIE1; -.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 1.50.10.10; -; 1.
DR   Gene3D; 2.70.98.40; Glycoside hydrolase, family 65, N-terminal domain; 1.
DR   Gene3D; 2.60.420.10; Maltose phosphorylase, domain 3; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR   InterPro; IPR005194; Glyco_hydro_65_C.
DR   InterPro; IPR005195; Glyco_hydro_65_M.
DR   InterPro; IPR005196; Glyco_hydro_65_N.
DR   InterPro; IPR037018; Glyco_hydro_65_N_sf.
DR   InterPro; IPR017045; Malt_Pase/Glycosyl_Hdrlase.
DR   PANTHER; PTHR11051; GLYCOSYL HYDROLASE-RELATED; 1.
DR   PANTHER; PTHR11051:SF14; MALTOSE PHOSPHORYLASE; 1.
DR   Pfam; PF03633; Glyco_hydro_65C; 1.
DR   Pfam; PF03632; Glyco_hydro_65m; 1.
DR   Pfam; PF03636; Glyco_hydro_65N; 1.
DR   PIRSF; PIRSF036289; Glycosyl_hydrolase_malt_phosph; 1.
DR   SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0000313|PDB:1H54, ECO:0007829|PDB:1H54}.
FT   DOMAIN          14..266
FT                   /note="Glycoside hydrolase family 65 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF03636"
FT   DOMAIN          323..679
FT                   /note="Glycoside hydrolase family 65 central catalytic"
FT                   /evidence="ECO:0000259|Pfam:PF03632"
FT   DOMAIN          689..746
FT                   /note="Glycoside hydrolase family 65 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF03633"
FT   ACT_SITE        487
FT                   /note="EMO_00068 proton donor"
FT                   /evidence="ECO:0007829|PDB:1H54"
FT   ACT_SITE        487
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR036289-50"
FT   BINDING         358..359
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR036289-51"
FT   BINDING         592..593
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR036289-51"
SQ   SEQUENCE   754 AA;  85737 MW;  F959BA54215C9D28 CRC64;
     MKRIFEVQPW NVITHTFDPK DKRLQESMTS LGNGYMGMRG DFEEGYSGDS LQGIYLGGVW
     YPDKTRVGWW KNGYPKYFGK VVNAVNFIKL PIEINGEPVD LAKDKISDFT LDLDMHQGVL
     NRSFVVERGA VRVALNFQRF LSVAQPELSV QKVTVKNLSD AEVDVTLKPS IDADVMNEEA
     NYDERFWDVL ATDQQADRGS IVAKTTPNPF GTPRFTSGME MRLVTDLKNV AITQPNEKEV
     TTAYTGKLAP QASAELEKRV IVVTSRDYDT QESLTAAMHQ LSDKVAQSSY EDLLNAHTAI
     WAQRWEKSDV VIKGDDESQQ GIRFNLFQLF STYYGEDARL NIGPKGFTGE KYGGATYWDT
     EAFAFPVYLG ITDPKVTRNL LMYRYKQLDG AYINAQEQGL KGALFPMVTF DGIECHNEWE
     ITFEEIHRNG DIAFAIYNYT RYTGDDSYVL HEGAKVLTEI SRFWADRVHF SKRNNQYMIH
     GVTGADEYEN NVDNNWDTNM LAQWTLKYTL EILGKVDQDT AKQLDVSDEE KTKWQDIVDR
     MYLPYDKDLN IFVQHDGFLD KDIEPVSSIP ADQRPINQNW SWDKILRSPY IKQGDVLQGI
     WDFIDDYTPE QKKANFDFYE PLTVHESSLS PAIHSVLAAD LHYEDKAVEL YSRTARLDLD
     NYNNDTTDGL HITSMTGAWI AVVQGFAGMR VRDGQLHYAP FLPKTWTSYT FRQVFRDRLI
     EVSVHADGPH FKLLSGEPLT IDVAGAAAAA AAAA
//