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Q7SID9

- Q7SID9_BREST

UniProt

Q7SID9 - Q7SID9_BREST

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Protein
Submitted name:

Oxidoreductase

Gene
N/A
Organism
Brevibacterium sterolicum
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei28 – 281FADImported
Binding sitei135 – 1351FAD; via amide nitrogenImported
Binding sitei150 – 1501FADImported
Binding sitei206 – 2061FAD; via carbonyl oxygenImported
Binding sitei211 – 2111FAD; via amide nitrogen and carbonyl oxygenImported
Active sitei259 – 2591Proton donor/acceptor
Binding sitei380 – 3801Cacodylate 1
Active sitei423 – 4231Proton donor/acceptor
Binding sitei425 – 4251Cacodylate 1
Binding sitei425 – 4251FADImported
Sitei425 – 4251Important for catalytic activity
Binding sitei490 – 4901Cacodylate 2
Binding sitei502 – 5021Cacodylate 1
Binding sitei527 – 5271Cacodylate 2

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi64 – 718FADImported
Nucleotide bindingi140 – 1434FADImported

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. UDP-N-acetylmuramate dehydrogenase activity Source: InterPro
Complete GO annotation...

Keywords - Ligandi

FADImported, Flavoprotein, Nucleotide-bindingImported

Names & Taxonomyi

Protein namesi
Submitted name:
OxidoreductaseImported
OrganismiBrevibacterium sterolicumImported
Taxonomic identifieri1702 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeBrevibacteriaceaeBrevibacterium

Pathology & Biotechi

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1I19X-ray1.70A/B1-561[»]
2I0KX-ray1.60A1-561[»]
ProteinModelPortaliQ7SID9.
SMRiQ7SID9. Positions 5-561.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7SID9.

Family & Domainsi

Sequence similaritiesi

Contains FAD-binding PCMH-type domain.SAAS annotation

Family and domain databases

Gene3Di1.10.45.10. 1 hit.
3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.40.462.10. 1 hit.
InterProiIPR015213. Cholesterol_OX_subst-bd.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR016164. FAD-linked_Oxase-like_C.
IPR006094. Oxid_FAD_bind_N.
IPR016170. V_Alc_oxidase/Cytok_DH_C_dom.
IPR016171. Vanillyl_alc_oxidase_C-sub2.
[Graphical view]
PfamiPF09129. Chol_subst-bind. 1 hit.
PF01565. FAD_binding_4. 1 hit.
[Graphical view]
SUPFAMiSSF55103. SSF55103. 1 hit.
SSF56176. SSF56176. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7SID9 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
STGPVAPLPT PPNFPNDIAL FQQAYQNWSK EIMLDATWVC SPKTPQDVVR
60 70 80 90 100
LANWAHEHDY KIRPRGAMHG WTPLTVEKGA NVEKVILADT MTHLNGITVN
110 120 130 140 150
TGGPVATVTA GAGASIEAIV TELQKHDLGW ANLPAPGVLS IGGALAVNAH
160 170 180 190 200
GAALPAVGQT TLPGHTYGSL SNLVTELTAV VWNGTTYALE TYQRNDPRIT
210 220 230 240 250
PLLTNLGRCF LTSVTMQAGP NFRQRCQSYT DIPWRELFAP KGADGRTFEK
260 270 280 290 300
FVAESGGAEA IWYPFTEKPW MKVWTVSPTK PDSSNEVGSL GSAGSLVGKP
310 320 330 340 350
PQAREVSGPY NYIFSDNLPE PITDMIGAIN AGNPGIAPLF GPAMYEITKL
360 370 380 390 400
GLAATNANDI WGWSKDVQFY IKATTLRLTE GGGAVVTSRA NIATVINDFT
410 420 430 440 450
EWFHERIEFY RAKGEFPLNG PVEIRCCGLD QAADVKVPSV GPPTISATRP
460 470 480 490 500
RPDHPDWDVA IWLNVLGVPG TPGMFEFYRE MEQWMRSHYN NDDATFRPEW
510 520 530 540 550
SKGWAFGPDP YTDNDIVTNK MRATYIEGVP TTENWDTARA RYNQIDPHRV
560
FTNGFMDKLL P
Length:561
Mass (Da):61,519
Last modified:December 15, 2003 - v1
Checksum:iD91FC2FD95D2102F
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1I19 X-ray 1.70 A/B 1-561 [» ]
2I0K X-ray 1.60 A 1-561 [» ]
ProteinModelPortali Q7SID9.
SMRi Q7SID9. Positions 5-561.
ModBasei Search...
MobiDBi Search...

Chemistry

DrugBanki DB03147. Flavin adenine dinucleotide.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q7SID9.

Family and domain databases

Gene3Di 1.10.45.10. 1 hit.
3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.40.462.10. 1 hit.
InterProi IPR015213. Cholesterol_OX_subst-bd.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR016164. FAD-linked_Oxase-like_C.
IPR006094. Oxid_FAD_bind_N.
IPR016170. V_Alc_oxidase/Cytok_DH_C_dom.
IPR016171. Vanillyl_alc_oxidase_C-sub2.
[Graphical view ]
Pfami PF09129. Chol_subst-bind. 1 hit.
PF01565. FAD_binding_4. 1 hit.
[Graphical view ]
SUPFAMi SSF55103. SSF55103. 1 hit.
SSF56176. SSF56176. 1 hit.
PROSITEi PS51387. FAD_PCMH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Oxygen access to the active site of cholesterol oxidase through a narrow channel is gated by an Arg-Glu pair."
    Coulombe R., Yue K.Q., Ghisla S., Vrielink A.
    J. Biol. Chem. 276:30435-30441(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) IN COMPLEX WITH FAD, ACTIVE SITE.
  2. "Structural and kinetic analyses of the H121A mutant of cholesterol oxidase."
    Lim L., Molla G., Guinn N., Ghisla S., Pollegioni L., Vrielink A.
    Biochem. J. 400:13-22(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) IN COMPLEX WITH FAD.

Entry informationi

Entry nameiQ7SID9_BREST
AccessioniPrimary (citable) accession number: Q7SID9
Entry historyi
Integrated into UniProtKB/TrEMBL: December 15, 2003
Last sequence update: December 15, 2003
Last modified: October 29, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3