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Q7SID9

- Q7SID9_BREST

UniProt

Q7SID9 - Q7SID9_BREST

Protein
Submitted name:

Oxidoreductase

Gene
N/A
Organism
Brevibacterium sterolicum
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (15 Dec 2003)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei28 – 281FADImported
    Binding sitei135 – 1351FAD; via amide nitrogenImported
    Binding sitei150 – 1501FADImported
    Binding sitei206 – 2061FAD; via carbonyl oxygenImported
    Binding sitei211 – 2111FAD; via amide nitrogen and carbonyl oxygenImported
    Active sitei259 – 2591Proton donor/acceptor
    Binding sitei380 – 3801Cacodylate 1
    Active sitei423 – 4231Proton donor/acceptor
    Binding sitei425 – 4251Cacodylate 1
    Binding sitei425 – 4251FADImported
    Sitei425 – 4251Important for catalytic activity
    Binding sitei490 – 4901Cacodylate 2
    Binding sitei502 – 5021Cacodylate 1
    Binding sitei527 – 5271Cacodylate 2

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi64 – 718FADImported
    Nucleotide bindingi140 – 1434FADImported

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. UDP-N-acetylmuramate dehydrogenase activity Source: InterPro

    Keywords - Ligandi

    FADImported, Flavoprotein, Nucleotide-bindingImported

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    OxidoreductaseImported
    OrganismiBrevibacterium sterolicumImported
    Taxonomic identifieri1702 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeBrevibacteriaceaeBrevibacterium

    Pathology & Biotechi

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1I19X-ray1.70A/B1-561[»]
    2I0KX-ray1.60A1-561[»]
    ProteinModelPortaliQ7SID9.
    SMRiQ7SID9. Positions 5-561.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ7SID9.

    Family & Domainsi

    Sequence similaritiesi

    Contains FAD-binding PCMH-type domain.SAAS annotation

    Family and domain databases

    Gene3Di1.10.45.10. 1 hit.
    3.30.43.10. 1 hit.
    3.30.465.10. 1 hit.
    3.40.462.10. 1 hit.
    InterProiIPR015213. Cholesterol_OX_subst-bd.
    IPR016169. CO_DH_flavot_FAD-bd_sub2.
    IPR016166. FAD-bd_2.
    IPR016167. FAD-bd_2_sub1.
    IPR016164. FAD-linked_Oxase-like_C.
    IPR006094. Oxid_FAD_bind_N.
    IPR016170. V_Alc_oxidase/Cytok_DH_C_dom.
    IPR016171. Vanillyl_alc_oxidase_C-sub2.
    [Graphical view]
    PfamiPF09129. Chol_subst-bind. 1 hit.
    PF01565. FAD_binding_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF55103. SSF55103. 1 hit.
    SSF56176. SSF56176. 1 hit.
    PROSITEiPS51387. FAD_PCMH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7SID9-1 [UniParc]FASTAAdd to Basket

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    STGPVAPLPT PPNFPNDIAL FQQAYQNWSK EIMLDATWVC SPKTPQDVVR    50
    LANWAHEHDY KIRPRGAMHG WTPLTVEKGA NVEKVILADT MTHLNGITVN 100
    TGGPVATVTA GAGASIEAIV TELQKHDLGW ANLPAPGVLS IGGALAVNAH 150
    GAALPAVGQT TLPGHTYGSL SNLVTELTAV VWNGTTYALE TYQRNDPRIT 200
    PLLTNLGRCF LTSVTMQAGP NFRQRCQSYT DIPWRELFAP KGADGRTFEK 250
    FVAESGGAEA IWYPFTEKPW MKVWTVSPTK PDSSNEVGSL GSAGSLVGKP 300
    PQAREVSGPY NYIFSDNLPE PITDMIGAIN AGNPGIAPLF GPAMYEITKL 350
    GLAATNANDI WGWSKDVQFY IKATTLRLTE GGGAVVTSRA NIATVINDFT 400
    EWFHERIEFY RAKGEFPLNG PVEIRCCGLD QAADVKVPSV GPPTISATRP 450
    RPDHPDWDVA IWLNVLGVPG TPGMFEFYRE MEQWMRSHYN NDDATFRPEW 500
    SKGWAFGPDP YTDNDIVTNK MRATYIEGVP TTENWDTARA RYNQIDPHRV 550
    FTNGFMDKLL P 561
    Length:561
    Mass (Da):61,519
    Last modified:December 15, 2003 - v1
    Checksum:iD91FC2FD95D2102F
    GO

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1I19 X-ray 1.70 A/B 1-561 [» ]
    2I0K X-ray 1.60 A 1-561 [» ]
    ProteinModelPortali Q7SID9.
    SMRi Q7SID9. Positions 5-561.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    DrugBanki DB03147. Flavin adenine dinucleotide.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q7SID9.

    Family and domain databases

    Gene3Di 1.10.45.10. 1 hit.
    3.30.43.10. 1 hit.
    3.30.465.10. 1 hit.
    3.40.462.10. 1 hit.
    InterProi IPR015213. Cholesterol_OX_subst-bd.
    IPR016169. CO_DH_flavot_FAD-bd_sub2.
    IPR016166. FAD-bd_2.
    IPR016167. FAD-bd_2_sub1.
    IPR016164. FAD-linked_Oxase-like_C.
    IPR006094. Oxid_FAD_bind_N.
    IPR016170. V_Alc_oxidase/Cytok_DH_C_dom.
    IPR016171. Vanillyl_alc_oxidase_C-sub2.
    [Graphical view ]
    Pfami PF09129. Chol_subst-bind. 1 hit.
    PF01565. FAD_binding_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55103. SSF55103. 1 hit.
    SSF56176. SSF56176. 1 hit.
    PROSITEi PS51387. FAD_PCMH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Oxygen access to the active site of cholesterol oxidase through a narrow channel is gated by an Arg-Glu pair."
      Coulombe R., Yue K.Q., Ghisla S., Vrielink A.
      J. Biol. Chem. 276:30435-30441(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) IN COMPLEX WITH FAD, ACTIVE SITE.
    2. "Structural and kinetic analyses of the H121A mutant of cholesterol oxidase."
      Lim L., Molla G., Guinn N., Ghisla S., Pollegioni L., Vrielink A.
      Biochem. J. 400:13-22(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) IN COMPLEX WITH FAD.

    Entry informationi

    Entry nameiQ7SID9_BREST
    AccessioniPrimary (citable) accession number: Q7SID9
    Entry historyi
    Integrated into UniProtKB/TrEMBL: December 15, 2003
    Last sequence update: December 15, 2003
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3