Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase

Gene

cobT

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB).UniRule annotation

Catalytic activityi

Beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole = nicotinate + alpha-ribazole 5'-phosphate.UniRule annotation

Pathwayi: alpha-ribazole biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-ribazole from 5,6-dimethylbenzimidazole.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT)
  2. no protein annotated in this organism
This subpathway is part of the pathway alpha-ribazole biosynthesis, which is itself part of Nucleoside biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-ribazole from 5,6-dimethylbenzimidazole, the pathway alpha-ribazole biosynthesis and in Nucleoside biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei304 – 3041Proton acceptorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-2071-MONOMER.
UniPathwayiUPA00061; UER00516.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferaseUniRule annotation (EC:2.4.2.21UniRule annotation)
Short name:
NN:DBI PRTUniRule annotation
Alternative name(s):
N(1)-alpha-phosphoribosyltransferaseUniRule annotation
Gene namesi
Name:cobTUniRule annotation
Ordered Locus Names:TTHB048
Encoded oniPlasmid pTT270 Publication
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Plasmid pTT27

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferasePRO_0000167074Add
BLAST

Structurei

Secondary structure

1
335
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1412Combined sources
Beta strandi16 – 183Combined sources
Turni20 – 234Combined sources
Helixi24 – 3714Combined sources
Beta strandi47 – 548Combined sources
Helixi57 – 615Combined sources
Helixi70 – 7910Combined sources
Helixi84 – 918Combined sources
Beta strandi94 – 1029Combined sources
Beta strandi113 – 1153Combined sources
Beta strandi119 – 1213Combined sources
Turni125 – 1273Combined sources
Helixi133 – 15220Combined sources
Beta strandi156 – 1638Combined sources
Helixi167 – 17913Combined sources
Helixi183 – 1864Combined sources
Turni190 – 1923Combined sources
Helixi195 – 21016Combined sources
Helixi218 – 2258Combined sources
Helixi228 – 24215Combined sources
Beta strandi247 – 2493Combined sources
Helixi252 – 26413Combined sources
Helixi266 – 2716Combined sources
Beta strandi272 – 2743Combined sources
Helixi282 – 2909Combined sources
Helixi306 – 32116Combined sources
Helixi327 – 3304Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J33X-ray2.00A1-335[»]
1WX1X-ray1.97A/B1-335[»]
ProteinModelPortaliQ7SIC7.
SMRiQ7SIC7. Positions 3-335.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7SIC7.

Family & Domainsi

Sequence similaritiesi

Belongs to the CobT family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000263499.
KOiK00768.
OMAiATFGGFE.
OrthoDBiEOG6HF61K.
PhylomeDBiQ7SIC7.

Family and domain databases

Gene3Di1.10.1610.10. 1 hit.
HAMAPiMF_00230. CobT.
InterProiIPR003200. Nict_dMeBzImd_PRibTrfase-like.
IPR023195. Nict_dMeBzImd_PRibTrfase_N.
IPR017846. Nict_dMeBzImd_PRibTrfase_pro.
[Graphical view]
PfamiPF02277. DBI_PRT. 1 hit.
[Graphical view]
SUPFAMiSSF52733. SSF52733. 1 hit.
TIGRFAMsiTIGR03160. cobT_DBIPRT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7SIC7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPEVFAQAR LRMDQLTKPP RALGYLEEVA LRLAALQGRV KPELGRGAVV
60 70 80 90 100
VAAADHGVVA EGVSAYPQEV TRQMVLNFLR GGAAINQFAL AADCAVYVLD
110 120 130 140 150
VGVVGELPDH PGLLKRKVRP GTANLAQGPA MTPEEAERAL LAGREAARRA
160 170 180 190 200
IAEGATLLAA GDMGIGNTTA AAALTAALLG LPPEAVVGRG TGVGEEGLRR
210 220 230 240 250
KRQAVARALA RLHPGMGPLE VAAEVGGLEL VAIAGIYLEG YEAGLPLVLD
260 270 280 290 300
GFPVTAGALL AWKMAPGLRD HLFAGHLSRE PGHRHQLEAL GLRPLLDLDL
310 320 330
ALGEGTGAVL AMPLLRAAAR ILHMATFQEA GVSRG
Length:335
Mass (Da):34,747
Last modified:June 7, 2005 - v2
Checksum:iB05DDF144305C4C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008227 Genomic DNA. Translation: BAD71844.1.
RefSeqiWP_008631396.1. NC_006462.1.
YP_145287.1. NC_006462.1.

Genome annotation databases

EnsemblBacteriaiBAD71844; BAD71844; BAD71844.
GeneIDi3169236.
KEGGittj:TTHB048.
PATRICi23959022. VBITheThe93045_1992.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008227 Genomic DNA. Translation: BAD71844.1.
RefSeqiWP_008631396.1. NC_006462.1.
YP_145287.1. NC_006462.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J33X-ray2.00A1-335[»]
1WX1X-ray1.97A/B1-335[»]
ProteinModelPortaliQ7SIC7.
SMRiQ7SIC7. Positions 3-335.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71844; BAD71844; BAD71844.
GeneIDi3169236.
KEGGittj:TTHB048.
PATRICi23959022. VBITheThe93045_1992.

Phylogenomic databases

HOGENOMiHOG000263499.
KOiK00768.
OMAiATFGGFE.
OrthoDBiEOG6HF61K.
PhylomeDBiQ7SIC7.

Enzyme and pathway databases

UniPathwayiUPA00061; UER00516.
BioCyciTTHE300852:GH8R-2071-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ7SIC7.

Family and domain databases

Gene3Di1.10.1610.10. 1 hit.
HAMAPiMF_00230. CobT.
InterProiIPR003200. Nict_dMeBzImd_PRibTrfase-like.
IPR023195. Nict_dMeBzImd_PRibTrfase_N.
IPR017846. Nict_dMeBzImd_PRibTrfase_pro.
[Graphical view]
PfamiPF02277. DBI_PRT. 1 hit.
[Graphical view]
SUPFAMiSSF52733. SSF52733. 1 hit.
TIGRFAMsiTIGR03160. cobT_DBIPRT. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.
  2. "Crystal structure of cobT from Thermus thermophilus HB8."
    RIKEN structural genomics initiative (RSGI)
    Submitted (JAN-2003) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Entry informationi

Entry nameiCOBT_THET8
AccessioniPrimary (citable) accession number: Q7SIC7
Secondary accession number(s): Q53WB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: June 7, 2005
Last modified: December 9, 2015
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Plasmid, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.