Reviewed, UniProtKB/Swiss-Prot Q7SIC1 (FUCL_ANGAN)

Names and origin

Protein names	Recommended name:     Fucolectin
Organism	Anguilla anguilla (European freshwater eel)
Taxonomic identifier	7936 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Anguilliformes › Anguillidae › Anguilla

Protein attributes

Sequence length	158 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as a defensive agent. Recognizes blood group fucosylated oligosaccharides including A, B, H and Lewis B-type antigens. Does not recognize Lewis A antigen and has low affinity for monovalent haptens. Ref.1 Ref.2
Subunit structure	Homotrimer. Ref.2
Subcellular location	Secreted By similarity.
Miscellaneous	Binds 1 calcium ion per monomer.
Sequence similarities	Belongs to the fucolectin family.

Ontologies

Keywords
Cellular component	Secreted
Ligand	Calcium Lectin Metal-binding
PTM	Disulfide bond
Technical term	3D-structure
Gene Ontology (GO)
Biological process	cell adhesion Inferred from electronic annotation. Source: InterPro regulation of cellular defense response Ref.2 Traceable author statement. Source: UniProtKB regulation of complement activation, lectin pathway Ref.2 Traceable author statement. Source: UniProtKB
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Ref.2 Inferred from direct assay. Source: UniProtKB fucose binding Ref.2 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 158

158

Fucolectin

PRO_0000223931

Regions

Region

16 – 148

133

F5/8 type C-like

Region

26 – 27

2

Antigenic glycan Ref.2

Region

82 – 83

2

Antigenic glycan Ref.2

Motif

79 – 81

3

Cell attachment site

Sites

Metal binding

35

1

Calcium; via carbonyl oxygen Ref.2

Metal binding

38

1

Calcium Ref.2

Metal binding

40

1

Calcium; via carbonyl oxygen Ref.2

Metal binding

49

1

Calcium Ref.2

Metal binding

146

1

Calcium; via carbonyl oxygen Ref.2

Metal binding

147

1

Calcium Ref.2

Binding site

46

1

Antigenic glycan Ref.2

Binding site

52

1

Antigenic glycan Ref.2

Binding site

79

1

Antigenic glycan Ref.2

Binding site

86

1

Antigenic glycan Ref.2

Amino acid modifications

Disulfide bond

50 ↔ 146

Ref.2

Disulfide bond

82 ↔ 83

Ref.2

Disulfide bond

108 ↔ 124

Ref.2

Secondary structure

1

.

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158

Helix Strand Turn

Details...

Beta strand

6 – 10

5

Helix

11 – 14

4

Beta strand

15 – 20

6

Helix

28 – 30

3

Helix

33 – 37

5

Helix

45 – 47

3

Beta strand

60 – 78

19

Beta strand

80 – 82

3

Turn

84 – 89

6

Beta strand

91 – 97

7

Turn

99 – 104

6

Beta strand

107 – 110

4

Beta strand

119 – 136

18

Beta strand

139 – 141

3

Beta strand

145 – 155

11

Sequences

	Sequence		Length	Mass (Da)
	Q7SIC1-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 80DCA6BB1E5AD701	FASTA	158	16,893
	10 20 30 40 50 60 VIPEGYTQEN VAVRGKATQS AQLRGEHAAN SEASNAIDGN RDSNFYHGSC THSSGQANPW 70 80 90 100 110 120 WRVDLLQVYT ITSVTITNRG DCCGERISGA EINIGQHLAS NGVNNPECSV IGSMATGETK 130 140 150 TFHCPAPMIG RYVVTYLPTS ESLHLCEVEV NVDKPAAA

References

[1]	"Lectinochemical studies on the affinity of Anguilla anguilla agglutinin for mammalian glycotopes." Wu A.M., Wu J.H., Singh T., Liu J.-H., Herp A. Life Sci. 75:1085-1103(2004) [PubMed: 15207656] [Abstract] Cited for: FUNCTION.
[2]	"A novel fucose recognition fold involved in innate immunity." Bianchet M.A., Odom E.W., Vasta G.R., Amzel L.M. Nat. Struct. Biol. 9:628-634(2002) [PubMed: 12091873] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEX WITH FUCOSE AND CALCIUM, FUNCTION, SUBUNIT.

Cross-references

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K12	X-ray	1.90	A	1-158	[»]

ModBase

Search...

Protein family/group databases

CAZy

CBM47. Carbohydrate-Binding Module Family 47.

Phylogenomic databases

HOVERGEN

Q7SIC1.

Family and domain databases

InterPro

IPR000421. Coagulation_factor_5/8-type_C.
IPR006585. FTP1.
[Graphical view]

Pfam

PF00754. F5_F8_type_C. 1 hit.
[Graphical view]

SMART

SM00607. FTP. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

FUCL_ANGAN

Accession

Primary (citable) accession number: Q7SIC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	December 15, 2003
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)