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Protein

Phosphoglycerate kinase 1

Gene

PGK1

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Glyceraldehyde-3-phosphate dehydrogenase (GAPDHS)
  2. Phosphoglycerate kinase 1 (PGK1), Phosphoglycerate kinase 2 (PGK2)
  3. no protein annotated in this organism
  4. Beta-enolase (ENO3)
  5. Pyruvate kinase (PKM), Pyruvate kinase, Pyruvate kinase
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39Substrate1 Publication1
Binding sitei123Substrate1 Publication1
Binding sitei171Substrate1 Publication1
Binding sitei220ATP1 Publication1
Binding sitei313ATP; via carbonyl oxygen1 Publication1
Binding sitei344ATP1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi373 – 376ATP1 Publication4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.2.3. 6170.
SABIO-RKQ7SIB7.
UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase 1 (EC:2.7.2.3)
Gene namesi
Name:PGK1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001458372 – 417Phosphoglycerate kinase 1Add BLAST416

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei4PhosphoserineBy similarity1
Modified residuei6N6-succinyllysineBy similarity1
Modified residuei11N6-acetyllysineBy similarity1
Modified residuei48N6-acetyllysine; alternateBy similarity1
Modified residuei48N6-succinyllysine; alternateBy similarity1
Modified residuei75N6-acetyllysineBy similarity1
Modified residuei76PhosphotyrosineBy similarity1
Modified residuei86N6-acetyllysineBy similarity1
Modified residuei91N6-acetyllysineBy similarity1
Modified residuei97N6-acetyllysineBy similarity1
Modified residuei131N6-acetyllysine; alternateBy similarity1
Modified residuei131N6-malonyllysine; alternateBy similarity1
Modified residuei146N6-acetyllysineBy similarity1
Modified residuei191N6-succinyllysineBy similarity1
Modified residuei196PhosphotyrosineBy similarity1
Modified residuei199N6-acetyllysineBy similarity1
Modified residuei203PhosphoserineBy similarity1
Modified residuei267N6-acetyllysineBy similarity1
Modified residuei291N6-acetyllysineBy similarity1
Modified residuei361N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ7SIB7.
PeptideAtlasiQ7SIB7.
PRIDEiQ7SIB7.

Interactioni

Subunit structurei

Monomer.2 Publications

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000013238.

Structurei

Secondary structure

1417
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 5Combined sources3
Helixi9 – 11Combined sources3
Beta strandi18 – 22Combined sources5
Beta strandi29 – 35Combined sources7
Helixi38 – 52Combined sources15
Beta strandi56 – 61Combined sources6
Turni73 – 75Combined sources3
Helixi79 – 89Combined sources11
Beta strandi93 – 95Combined sources3
Beta strandi99 – 101Combined sources3
Helixi102 – 109Combined sources8
Beta strandi115 – 118Combined sources4
Helixi122 – 124Combined sources3
Turni126 – 130Combined sources5
Beta strandi131 – 133Combined sources3
Beta strandi139 – 141Combined sources3
Helixi144 – 156Combined sources13
Beta strandi159 – 163Combined sources5
Helixi166 – 168Combined sources3
Helixi174 – 177Combined sources4
Beta strandi184 – 186Combined sources3
Helixi188 – 201Combined sources14
Beta strandi206 – 212Combined sources7
Helixi218 – 220Combined sources3
Helixi221 – 228Combined sources8
Beta strandi232 – 236Combined sources5
Helixi238 – 240Combined sources3
Helixi241 – 249Combined sources9
Helixi262 – 264Combined sources3
Helixi266 – 276Combined sources11
Beta strandi279 – 281Combined sources3
Beta strandi285 – 293Combined sources9
Beta strandi298 – 302Combined sources5
Turni303 – 305Combined sources3
Beta strandi312 – 316Combined sources5
Helixi318 – 330Combined sources13
Beta strandi332 – 338Combined sources7
Helixi346 – 348Combined sources3
Helixi350 – 364Combined sources15
Beta strandi368 – 371Combined sources4
Helixi375 – 382Combined sources8
Turni386 – 388Combined sources3
Beta strandi389 – 392Combined sources4
Helixi396 – 403Combined sources8
Helixi409 – 412Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HDIX-ray1.80A5-417[»]
1KF0X-ray2.50A2-417[»]
1VJCX-ray2.10A2-417[»]
1VJDX-ray1.90A2-417[»]
ProteinModelPortaliQ7SIB7.
SMRiQ7SIB7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7SIB7.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 26Substrate binding3
Regioni63 – 66Substrate binding4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

eggNOGiKOG1367. Eukaryota.
COG0126. LUCA.
HOGENOMiHOG000227107.
HOVERGENiHBG008177.
InParanoidiQ7SIB7.
KOiK00927.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7SIB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSNKLTLD KLDVKGKRVV MRVDFNVPMK NNQITNNQRI KAAIPSIKFC
60 70 80 90 100
LDNGAKSVVL MSHLGRPDGI PMPDKYSLEP VAVELKSLPG KDVLFLKDCV
110 120 130 140 150
GPEVEKACAD PAAGSVILLE NLRFHVEEEG KGKDASGSKV KADPAKIEAF
160 170 180 190 200
RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPKKAGGFLM KKELNYFAKA
210 220 230 240 250
LESPERPFLA ILGGAKVADK IQLINNMLDK VNEMIIGGGM AFTFLKVLNN
260 270 280 290 300
MEIGTSLFDE EGSKIVKDLM SKAEKNGVKI TLPVDFVTAD KFDENAKIGQ
310 320 330 340 350
ATVASGIPAG WMGLDCGPES SKKYSEAVAR AKQIVWNGPV GVFEWEAFAQ
360 370 380 390 400
GTKALMDEVV KATSRGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL
410
ELLEGKVLPG VDALSNV
Length:417
Mass (Da):44,559
Last modified:January 23, 2007 - v3
Checksum:iB014E769058C1135
GO

Sequence cautioni

The sequence described in PubMed:12102622 differs from that shown. The sequence shown in PDB entry 1KF0 is a tentative sequence based on the electron density. It differs from that shown in 14 positions.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY677198 mRNA. Translation: AAT77773.1.
RefSeqiNP_001093402.1. NM_001099932.1.
UniGeneiSsc.23800.

Genome annotation databases

GeneIDi407608.
KEGGissc:407608.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY677198 mRNA. Translation: AAT77773.1.
RefSeqiNP_001093402.1. NM_001099932.1.
UniGeneiSsc.23800.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HDIX-ray1.80A5-417[»]
1KF0X-ray2.50A2-417[»]
1VJCX-ray2.10A2-417[»]
1VJDX-ray1.90A2-417[»]
ProteinModelPortaliQ7SIB7.
SMRiQ7SIB7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000013238.

Proteomic databases

PaxDbiQ7SIB7.
PeptideAtlasiQ7SIB7.
PRIDEiQ7SIB7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi407608.
KEGGissc:407608.

Organism-specific databases

CTDi5230.

Phylogenomic databases

eggNOGiKOG1367. Eukaryota.
COG0126. LUCA.
HOGENOMiHOG000227107.
HOVERGENiHBG008177.
InParanoidiQ7SIB7.
KOiK00927.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.
BRENDAi2.7.2.3. 6170.
SABIO-RKQ7SIB7.

Miscellaneous databases

EvolutionaryTraceiQ7SIB7.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGK1_PIG
AccessioniPrimary (citable) accession number: Q7SIB7
Secondary accession number(s): Q6B6L7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 89 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.