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Protein

CCA-adding enzyme

Gene

cca

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.

Catalytic activityi

A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei27ATP or CTP; via amide nitrogen1
Binding sitei30ATP or CTP1
Metal bindingi40MagnesiumBy similarity1
Metal bindingi42MagnesiumBy similarity1
Binding sitei111ATP or CTP1
Sitei112May assist in discriminating ATP from CTP1
Sitei153Involved in nucleotide selectionSequence analysis1
Binding sitei154ATP or CTP1
Binding sitei157ATP or CTP1
Binding sitei160ATP or CTP1
Binding sitei163ATP or CTP1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

RNA repair, tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CCA-adding enzyme (EC:2.7.7.72)
Alternative name(s):
CCA tRNA nucleotidyltransferase
tRNA CCA-pyrophosphorylase
tRNA adenylyl-/cytidylyl- transferase
tRNA nucleotidyltransferase
tRNA-NT
Gene namesi
Name:cca
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001390331 – 404CCA-adding enzymeAdd BLAST404

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1404
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 17Combined sources15
Beta strandi22 – 26Combined sources5
Helixi27 – 34Combined sources8
Beta strandi42 – 47Combined sources6
Helixi49 – 55Combined sources7
Beta strandi57 – 63Combined sources7
Helixi64 – 66Combined sources3
Beta strandi68 – 73Combined sources6
Beta strandi76 – 82Combined sources7
Beta strandi84 – 86Combined sources3
Beta strandi98 – 100Combined sources3
Helixi104 – 109Combined sources6
Beta strandi111 – 114Combined sources4
Helixi115 – 117Combined sources3
Beta strandi129 – 131Combined sources3
Helixi132 – 138Combined sources7
Beta strandi143 – 145Combined sources3
Helixi147 – 153Combined sources7
Helixi156 – 168Combined sources13
Helixi174 – 183Combined sources10
Helixi184 – 189Combined sources6
Helixi192 – 203Combined sources12
Beta strandi205 – 207Combined sources3
Helixi208 – 217Combined sources10
Turni219 – 222Combined sources4
Helixi231 – 236Combined sources6
Helixi237 – 239Combined sources3
Helixi242 – 244Combined sources3
Helixi248 – 258Combined sources11
Helixi264 – 270Combined sources7
Helixi275 – 290Combined sources16
Helixi294 – 296Combined sources3
Helixi299 – 321Combined sources23
Helixi326 – 336Combined sources11
Helixi344 – 346Combined sources3
Helixi351 – 358Combined sources8
Beta strandi362 – 364Combined sources3
Helixi365 – 378Combined sources14
Helixi386 – 397Combined sources12
Helixi399 – 403Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MIVX-ray3.50A/B1-404[»]
1MIWX-ray3.00A/B1-404[»]
1MIYX-ray3.52A/B1-404[»]
ProteinModelPortaliQ7SIB1.
SMRiQ7SIB1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7SIB1.

Family & Domainsi

Domaini

The crystal structure reveals a seahorse-shaped subunit consisting of four domains: head, neck, body, and tail. The head domain contains the nucleotidyltransferase activity. The neck domain provides a specific template for the incoming ATP or CTP, whereas the body and tail domains may be involved in tRNA binding.

Sequence similaritiesi

Family and domain databases

CDDicd05398. NT_ClassII-CCAase. 1 hit.
HAMAPiMF_01263. CCA_bact_type3. 1 hit.
InterProiIPR032810. CCA-adding_enz_C.
IPR023068. CCA-adding_enz_firmicutes.
IPR002646. PolA_pol_head_dom.
IPR032828. PolyA_RNA-bd.
[Graphical view]
PfamiPF01743. PolyA_pol. 1 hit.
PF12627. PolyA_pol_RNAbd. 1 hit.
PF13735. tRNA_NucTran2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7SIB1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPPFQEALG IIQQLKQHGY DAYFVGGAVR DLLLGRPIGD VDIATSALPE
60 70 80 90 100
DVMAIFPKTI DVGSKHGTVV VVHKGKAYEV TTFKTDGDYE DYRRPESVTF
110 120 130 140 150
VRSLEEDLKR RDFTMNAIAM DEYGTIIDPF GGREAIRRRI IRTVGEAEKR
160 170 180 190 200
FREDALRMMR AVRFVSELGF ALAPDTEQAI VQNAPLLAHI SVERMTMEME
210 220 230 240 250
KLLGGPFAAR ALPLLAETGL NAYLPGLAGK EKQLRLAAAY RWPWLAAREE
260 270 280 290 300
RWALLCHALG VQESRPFLRA WKLPNKVVDE AGAILTALAD IPRPEAWTNE
310 320 330 340 350
QLFSAGLERA LSVETVRAAF TGAPPGPWHE KLRRRFASLP IKTKGELAVN
360 370 380 390 400
GKDVIEWVGK PAGPWVKEAL DAIWRAVVNG EVENEKERIY AWLMERNRTR

EKNC
Length:404
Mass (Da):45,369
Last modified:December 15, 2003 - v1
Checksum:iDC0C8E4C47133940
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MIVX-ray3.50A/B1-404[»]
1MIWX-ray3.00A/B1-404[»]
1MIYX-ray3.52A/B1-404[»]
ProteinModelPortaliQ7SIB1.
SMRiQ7SIB1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ7SIB1.

Family and domain databases

CDDicd05398. NT_ClassII-CCAase. 1 hit.
HAMAPiMF_01263. CCA_bact_type3. 1 hit.
InterProiIPR032810. CCA-adding_enz_C.
IPR023068. CCA-adding_enz_firmicutes.
IPR002646. PolA_pol_head_dom.
IPR032828. PolyA_RNA-bd.
[Graphical view]
PfamiPF01743. PolyA_pol. 1 hit.
PF12627. PolyA_pol_RNAbd. 1 hit.
PF13735. tRNA_NucTran2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCCA_GEOSE
AccessioniPrimary (citable) accession number: Q7SIB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: December 15, 2003
Last modified: November 2, 2016
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.