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Reviewed, UniProtKB/Swiss-Prot Q7SIA2 (SYEP_CRIGR)

Last modified June 16, 2009. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Bifunctional aminoacyl-tRNA synthetase
Including the following 2 domains:
    1- Recommended name:
            Glutamyl-tRNA synthetase
              EC=6.1.1.17
        Alternative name(s):
            Glutamate--tRNA ligase
    2- Recommended name:
            Prolyl-tRNA synthetase
              EC=6.1.1.15
        Alternative name(s):
            Proline--tRNA ligase
Gene names
Name: EPRS
Synonyms: QPRS
OrganismCricetulus griseus (Chinese hamster)
Taxonomic identifier10029 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Protein attributes

Sequence length49 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA By similarity.

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).

Subunit structure

Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins, p18, p48 and p43. Interacts with DUS2L By similarity.

Domain

The WHEP-TRS domain is involved in RNA binding.

Sequence similarities

Contains at least 1 WHEP-TRS domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›49›49Bifunctional aminoacyl-tRNA synthetase
PRO_0000119742

Regions

Domain‹1 – ›49›49WHEP-TRS

Amino acid modifications

Modified residue461Phosphotyrosine By similarity

Experimental info

Non-terminal residue11
Non-terminal residue491

Secondary structure

....... 49
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q7SIA2-1 [UniParc].

Last modified July 19, 2004. Version 2.
Checksum: A8B609A53C7E695A

FASTA495,409
        10         20         30         40 
YDKIAAQGEV VRKLKAEKAP KAKVTEAVEC LLSLKAEYKE KTGKEYVPG 

« Hide

References

[1]"A recurrent RNA-binding domain is appended to eukaryotic aminoacyl-tRNA synthetases."
Cahuzac B., Berthonneau E., Birlirakis N., Guittet E., Mirande M.
EMBO J. 19:445-452(2000) [PubMed: 10654942] [Abstract]
Cited for: STRUCTURE BY NMR OF 1-49, RNA-BINDING SITE.

Cross-references

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1D2DNMR-A1-49[»]
1R1BNMR-A1-49[»]
ModBaseSearch...

Phylogenomic databases

HOVERGENQ7SIA2.

Enzyme and pathway databases

BRENDA6.1.1.15. 18.
6.1.1.17. 18.

Family and domain databases

InterProIPR009068. S15_NS1_RNA_bd.
IPR000738. WHEP-TRS.
[Graphical view]
Gene3DG3DSA:1.10.287.10. S15_NS1_RNA_bd. 1 hit.
PfamPF00458. WHEP-TRS. 1 hit.
[Graphical view]
PROSITEPS00762. WHEP_TRS_1. Partial match.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYEP_CRIGR
AccessionPrimary (citable) accession number: Q7SIA2
Secondary accession number(s): Q7SIG9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: June 16, 2009
This is version 31 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents