Q7SIA2 (SYEP_CRIGR) Reviewed, UniProtKB/Swiss-Prot
Last modified November 13, 2013. Version 47. History...
Names and origin
|Protein names||Recommended name:|
Bifunctional glutamate/proline--tRNA ligase
Bifunctional aminoacyl-tRNA synthetase
|Organism||Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)|
|Taxonomic identifier||10029 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus|
|Sequence length||49 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the multisynthetase complex and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation By similarity.
ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).
ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).
Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins, p18, p48 and p43. Interacts with DUS2L. Component of the GAIT complex By similarity.
The WHEP-TRS domain is involved in RNA binding.
Phosphorylated in a IFN-gamma-dependent manner; the phosphorylation is causing release from the multisynthetase complex, association with the GAIT complex and subsequent involvement in transcript-selective translation inhibition.
Contains at least 1 WHEP-TRS domain.
|Biological process||Protein biosynthesis|
|Molecular function||Aminoacyl-tRNA synthetase|
|Gene Ontology (GO)|
|Biological_process||regulation of translation|
Inferred from electronic annotation. Source: UniProtKB-KWtRNA aminoacylation for protein translation
Inferred from electronic annotation. Source: InterPro
Inferred from electronic annotation. Source: UniProtKB-KWRNA stem-loop binding
Inferred from sequence or structural similarity. Source: UniProtKBglutamate-tRNA ligase activity
Inferred from electronic annotation. Source: UniProtKB-ECproline-tRNA ligase activity
Inferred from electronic annotation. Source: UniProtKB-EC
|Complete GO annotation...|
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||‹1 – ›49||›49||Bifunctional glutamate/proline--tRNA ligase||PRO_0000119742|
|Domain||‹1 – ›49||›49||WHEP-TRS|
Amino acid modifications
|Modified residue||46||1||Phosphotyrosine By similarity|
Helix Strand Turn
|Helix||2 – 16||15|
|Helix||21 – 38||18|
|Beta strand||40 – 45||6|
3D structure databases
|SMR||Q7SIA2. Positions 1-49. |
Protocols and materials databases
Family and domain databases
|Gene3D||1.10.287.10. 1 hit. |
|InterPro||IPR009068. S15_NS1_RNA-bd. |
|Pfam||PF00458. WHEP-TRS. 1 hit. |
|SMART||SM00991. WHEP-TRS. 1 hit. |
|SUPFAM||SSF47060. SSF47060. 1 hit. |
|PROSITE||PS00762. WHEP_TRS_1. Partial match. |
PS51185. WHEP_TRS_2. 1 hit.
|Accession||Primary (citable) accession number: Q7SIA2|
Secondary accession number(s): Q7SIG9
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|