Q7SI98 (Q7SI98_STROI) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: Hydrolase PDB 1XYF PDB 1ISV PDB 1ISW PDB 1ISX PDB 1ISY PDB 1ISZ PDB 1IT0
Organism	Streptomyces olivaceoviridis (Streptomyces corchorusii) PDB 1XYF PDB 1ISV PDB 1ISW PDB 1ISX PDB 1ISY PDB 1ISZ PDB 1IT0
Taxonomic identifier	1921 [NCBI]
Taxonomic lineage	Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the glycosyl hydrolase 10 (cellulase F) family. RuleBase RU003432

Ontologies

Keywords
Molecular function	Glycosidase RuleBase RU003432 Hydrolase
Technical term	3D-structure PDB 1XYF PDB 1ISV PDB 1ISW PDB 1ISX PDB 1ISY PDB 1ISZ PDB 1IT0 PDB 2G3J PDB 1V6W PDB 1V6Y PDB 2D23 PDB 2D1Z PDB 2G3I PDB 2G4F PDB 2D22 PDB 2D20 PDB 2D24 PDB 1V6V PDB 1V6U PDB 1V6X
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	325 – 328	4	Galactose binding PDB 1ISZ
	Region	408 – 411	4	Galactose binding PDB 1ISZ
Sites
	Binding site	338	1	Galactose PDB 1ISZ
	Binding site	343	1	Galactose PDB 1ISZ
	Binding site	347	1	Galactose PDB 1ISZ
	Binding site	430	1	Galactose PDB 1ISZ

Sequences

	Sequence		Length	Mass (Da)
	Q7SI98 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 6F26B9EC33801083	FASTA	436	46,749
	10 20 30 40 50 60 AESTLGAAAA QSGRYFGTAI ASGKLGDSAY TTIASREFNM VTAENEMKID ATEPQRGQFN 70 80 90 100 110 120 FSAGDRVYNW AVQNGKQVRG HTLAWHSQQP GWMQSLSGST LRQAMIDHIN GVMGHYKGKI 130 140 150 160 170 180 AQWDVVNEAF SDDGSGGRRD SNLQRTGNDW IEVAFRTARA ADPAAKLCYN DYNIENWTWA 190 200 210 220 230 240 KTQGVYNMVR DFKQRGVPID CVGFQSHFNS GSPYNSNFRT TLQNFAALGV DVAITELDIQ 250 260 270 280 290 300 GASSSTYAAV TNDCLAVSRC LGITVWGVRD TDSWRSGDTP LLFNGDGSKK AAYTAVLNAL 310 320 330 340 350 360 NGGSSTPPPS GGGQIKGVGS GRCLDVPNAS TTDGTQVQLY DCHSATNQQW TYTDAGELRV 370 380 390 400 410 420 YGDKCLDAAG TGNGTKVQIY SCWGGDNQKW RLNSDGSIVG VQSGLCLDAV GGGTANGTLI 430 QLYSCSNGSN QRWTRT

References

[1]	"Crystal structures of the sugar complexes of Streptomyces olivaceoviridis E-86 xylanase: sugar binding structure of the family 13 carbohydrate binding module." Fujimoto Z., Kuno A., Kaneko S., Kobayashi H., Kusakabe I., Mizuno H. J. Mol. Biol. 316:65-78(2002) [PubMed: 11829503] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH GALACTOSE.
[2]	"Crystal structures of decorated xylooligosaccharides bound to a family 10 xylanase from Streptomyces olivaceoviridis E-86." Fujimoto Z., Kaneko S., Kuno A., Kobayashi H., Kusakabe I., Mizuno H. J. Biol. Chem. 279:9606-9614(2004) [PubMed: 14670957] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS).
[3]	"Structure and function of a family 10 beta-xylanase chimera of Streptomyces olivaceoviridis E-86 FXYN and Cellulomonas fimi Cex." Kaneko S., Ichinose H., Fujimoto Z., Kuno A., Yura K., Go M., Mizuno H., Kusakabe I., Kobayashi H. J. Biol. Chem. 279:26619-26626(2004) [PubMed: 15078885] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 1-239.
[4]	"Variants of S. olivaceoviridis xylanase." Diertavitian S., Kaneko S., Fujimoto Z., Kuno A., Johansson E., Lo Leggio L. Submitted (FEB-2006) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 1-303.
[5]	"Crystarographic snapshots of every step in the cleavage of a natural substrate by a retaining xylanase from Streptomyces." Suzuki R., Kuno A., Fujimoto Z., Ito S., Kawahara S.I., Kaneko S., Hasegawa T., Taira K. Submitted (SEP-2005) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS).
[6]	"Crystal structure of Streptomyces olivaceoviridis E-86 beta-xylanase containing xylan-binding domain." Fujimoto Z., Kuno A., Kaneko S., Yoshida S., Kobayashi H., Kusakabe I., Mizuno H. J. Mol. Biol. 300:575-585(2000) [PubMed: 10884353] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS).
[7]	"Variants of S.olivaceoviridis xylanase." Diertavitian S., Kaneko S., Fujimoto Z., Kuno A., Johansson E., Lo Leggio L. Submitted (FEB-2006) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) OF 1-302.
+	Additional computationally mapped references.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ISV	X-ray	2.10	A/B	1-436	[»]
1ISW	X-ray	2.10	A/B	1-436	[»]
1ISX	X-ray	2.10	A/B	1-436	[»]
1ISY	X-ray	2.10	A/B	1-436	[»]
1ISZ	X-ray	2.00	A/B	1-436	[»]
1IT0	X-ray	2.00	A/B	1-436	[»]
1V6U	X-ray	2.10	A/B	1-436	[»]
1V6V	X-ray	2.10	A/B	1-436	[»]
1V6W	X-ray	2.00	A/B	1-436	[»]
1V6X	X-ray	2.10	A/B	1-436	[»]
1V6Y	X-ray	2.20	A	1-239	[»]
1XYF	X-ray	1.90	A/B	1-436	[»]
2D1Z	X-ray	1.60	A/B	1-436	[»]
2D20	X-ray	1.85	A/B	1-436	[»]
2D22	X-ray	1.70	A/B	1-436	[»]
2D23	X-ray	1.95	A/B	1-436	[»]
2D24	X-ray	1.85	A/B	1-436	[»]
2G3I	X-ray	2.10	A	1-303	[»]
2G3J	X-ray	2.70	A	1-303	[»]
2G4F	X-ray	2.65	A/B	1-302	[»]

ProteinModelPortal

Q7SI98.

SMR

Q7SI98. Positions 1-436.

ModBase

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Protein family/group databases

CAZy

CBM13. Carbohydrate-Binding Module Family 13.
GH10. Glycoside Hydrolase Family 10.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR001000. Glyco_hydro_10.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
IPR008997. Ricin_B-rel_lectin.
IPR000772. Ricin_B_lectin.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.

Pfam

PF00331. Glyco_hydro_10. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]

PRINTS

PR00134. GLHYDRLASE10.

SMART

SM00633. Glyco_10. 1 hit.
SM00458. RICIN. 1 hit.
[Graphical view]

SUPFAM

SSF51445. Glyco_hydro_cat. 1 hit.
SSF50370. RicinB_like. 1 hit.

PROSITE

PS00591. GLYCOSYL_HYDROL_F10. 1 hit.
PS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]

ProtoNet

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Entry information

Entry name

Q7SI98_STROI

Accession

Primary (citable) accession number: Q7SI98
Secondary accession number(s): Q7SID1

Entry history

Integrated into UniProtKB/TrEMBL:	December 15, 2003
Last sequence update:	December 15, 2003
Last modified:	July 27, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)