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Q7SHZ2 (GATA_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial

Short name=Glu-AdT subunit A
EC=6.3.5.7
Gene names
ORF Names:NCU00660
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]
Taxonomic identifier367110 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora

Protein attributes

Sequence length542 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_03150

Catalytic activity

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_03150

Subunit structure

Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A, B and C subunits By similarity.

Subcellular location

Mitochondrion By similarity HAMAP-Rule MF_03150.

Sequence similarities

Belongs to the amidase family. GatA subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentMitochondrion
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtranslation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentmitochondrion

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 542542Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial HAMAP-Rule MF_03150
PRO_0000413356

Sites

Active site551Charge relay system By similarity
Active site1431Charge relay system By similarity
Active site1671Acyl-ester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7SHZ2 [UniParc].

Last modified February 19, 2014. Version 2.
Checksum: E7EA772A51C082A3

FASTA54258,740
        10         20         30         40         50         60 
MSARIGRLRG LCLRHVSSTR QTSPIQRRSL NQFISRVQQH DQPALEQRQF RLAVKDNIAT 

        70         80         90        100        110        120 
VSPNSEEETP LTTTCGSNFL ANYRSPFEAT IVSQLRSRGA LLVGKTNLDE FGMGSHSVHT 

       130        140        150        160        170        180 
AFGAVAQEGE HEQQAVKHSA GGSSGGSAVA VATGEADIAL GTDTGGSVRL PAGYTGVVGF 

       190        200        210        220        230        240 
KPSYGMLSRY GVVPYANSLD TVGLLAKEVR PIAELILGGA DKVTRGLWAE HDPLDPTSLS 

       250        260        270        280        290        300 
HGARRRCASQ RDCYTGPLPK DPYPLKNLKF GLPLEYNITE LSPSIRHSWS AAAAKLQSLG 

       310        320        330        340        350        360 
ARLVPVSLPS TRHALSAYYV IAPAEASSNL AKYDGIRYGT RFTSPTESDA AISEGPEGRE 

       370        380        390        400        410        420 
EEEEGILYAR ARTAGFGDEV KRRILLGAYT LSSAAMDNYF LKAQKVRRLV RRDFNRVFAL 

       430        440        450        460        470        480 
PNPLLDKPER FELSELPETV GLEDKWGPTE VDFLLCPTAP TTAPRLDEVL SEEEKDPVSA 

       490        500        510        520        530        540 
YMNDVFTVPA SLAGLPAISV PMRVEDKEGA GMAGLQLIGQ YWDDARLLAV AETVADVVRE 


EL 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CM002236 Genomic DNA. Translation: EAA36564.2.
RefSeqXP_965800.1. XM_960707.1.
UniGeneNcr.25891.

3D structure databases

ProteinModelPortalQ7SHZ2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5141.NCU00660.1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFNCRT00000000714; EFNCRP00000000714; EFNCRG00000000714.
GeneID3881925.
KEGGncr:NCU00660.

Phylogenomic databases

eggNOGCOG0154.
HOGENOMHOG000116699.
KOK02433.
OrthoDBEOG7P8PJ3.

Family and domain databases

Gene3D3.90.1300.10. 1 hit.
HAMAPMF_00120. GatA.
InterProIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERPTHR11895. PTHR11895. 1 hit.
PfamPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMSSF75304. SSF75304. 2 hits.
PROSITEPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGATA_NEUCR
AccessionPrimary (citable) accession number: Q7SHZ2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: February 19, 2014
Last modified: April 16, 2014
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families