Q7SHZ2 (GATA_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial
Short name=Glu-AdT subunit A
EC=6.3.5.-

Gene names

ORF Names:

NCU00660

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora › Neurospora crassa

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_03150
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_03150
Subunit structure	Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A, B and C subunits By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the amidase family. GatA subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutaminyl-tRNAGln biosynthesis via transamidation Inferred from electronic annotation. Source: HAMAP mitochondrial translation Inferred from electronic annotation. Source: HAMAP
Cellular_component	glutamyl-tRNA(Gln) amidotransferase complex Inferred from electronic annotation. Source: HAMAP mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 526	526	Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial HAMAP-Rule MF_03150		PRO_0000413356
Sites
	Active site	39	1	Charge relay system By similarity
	Active site	127	1	Charge relay system By similarity
	Active site	151	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7SHZ2 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 9A1299884908E7DD	FASTA	526	56,843
	10 20 30 40 50 60 MSARIGRLRG LCLRHSSWSV VQQHDQPALE QRQFRLAVKD NIATVSPNSE EETPLTTTCG 70 80 90 100 110 120 SNFLANYRSP FEATIVSQLR SRGALLVGKT NLDEFGMGSH SVHTAFGAVA QEGEHEQQAV 130 140 150 160 170 180 KHSAGGSSGG SAVAVATGEA DIALGTDTGG SVRLPAGYTG VVGFKPSYGM LSRYGVVPYA 190 200 210 220 230 240 NSLDTVGLLA KEVRPIAELI LGGADKVTRG LWAEHDPLDP TSLSHGARRR CASQRDCYTG 250 260 270 280 290 300 PLPKDPYPLK NLKFGLPLEY NITELSPSIR HSWSAAAAKL QSLGARLVPV SLPSTRHALS 310 320 330 340 350 360 AYYVIAPAEA SSNLAKYDGI RYGTRFTSPT ESDAAISEGP EGREEEEEGI LYARARTAGF 370 380 390 400 410 420 GDEVKRRILL GAYTLSSAAM DNYFLKAQKV RRLVRRDFNR VFALPNPLLD KPERFELSEL 430 440 450 460 470 480 PETVGLEDKW GPTEVDFLLC PTAPTTAPRL DEVLSEEEKD PVSAYMNDVF TVPASLAGLP 490 500 510 520 AISVPMRVED KEGAGMAGLQ LIGQYWDDAR LLAVAETVAD VVREEL

References

[1]

"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AABX02000002 Genomic DNA. Translation: EAA36564.1.
RefSeq	XP_965800.1. XM_960707.1.
UniGene	Ncr.25891.
3D structure databases
ProteinModelPortal	Q7SHZ2.
ModBase	Search...
Protein-protein interaction databases
STRING	5141.NCU00660.1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000000714; EFNCRP00000000714; EFNCRG00000000714.
GeneID	3881925.
KEGG	ncr:NCU00660.
Phylogenomic databases
eggNOG	COG0154.
HOGENOM	HOG000116699.
KO	K02433.
OrthoDB	EOG4MKRR3.
Family and domain databases
Gene3D	3.90.1300.10. 1 hit.
HAMAP	MF_00120. GatA.
InterPro	IPR000120. Amidase. IPR020556. Amidase_CS. IPR023631. Amidase_dom. IPR004412. GatA. [Graphical view]
PANTHER	PTHR11895. PTHR11895. 1 hit.
Pfam	PF01425. Amidase. 1 hit. [Graphical view]
SUPFAM	SSF75304. Amidase_sig_enz. 1 hit.
PROSITE	PS00571. AMIDASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATA_NEUCR

Accession

Primary (citable) accession number: Q7SHZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2011
Last sequence update:	December 15, 2003
Last modified:	April 3, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program