Q7SFX6 (LYS1_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Saccharopine dehydrogenase [NAD+, L-lysine-forming]
Short name=SDH
EC=1.5.1.7

Alternative name(s):
Lysine--2-oxoglutarate reductase

Gene names

ORF Names:

NCU03118

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O = L-lysine + 2-oxoglutarate + NADH.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 3/3.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the AlaDH/PNT family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Lysine biosynthesis
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lysine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	saccharopine dehydrogenase (NAD+, L-lysine-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Saccharopine dehydrogenase [NAD+, L-lysine-forming]		PRO_0000199013
Sites
	Active site	202	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7SFX6 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 7300490C46C40592	FASTA	372	40,877
	10 20 30 40 50 60 MAPTVLHLRS ETKHLEHRSA LTPTTTAELI KAGYIVNVER SPERIFDDEE FEKAGATLVP 70 80 90 100 110 120 EHSWVDAPKE HIIVGLKELE EKDFPLKHVH VQFAHCYKQQ AGWENVLARF PRGGGTLLDL 130 140 150 160 170 180 EFLVDEHGRR VAAFGFHAGF AGAALALEVW AWQLNHSEPF PGVESYPNED ALIADVKKAV 190 200 210 220 230 240 KEGVEAAGRL PRVIVIGARG RCGSGAVSAL KKAGIPDENI LDWDMAETAK GGPFKEITDS 250 260 270 280 290 300 DIFVNCIYLT SKIPNFVNME SLQVPDRQLR VVCDVSADTT SPFTPVPIYT VATTFDKPTV 310 320 330 340 350 360 PVDGLTSGPP LSVISIDHLP SLLPREASEA FSHDLLPSLL TLNDWQNSPV WARAKQLFDE 370 KVATLPESAL QK

References

[1]

"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AABX02000009 Genomic DNA. Translation: EAA35741.1.
RefSeq	XP_964977.1. XM_959884.2.
UniGene	Ncr.23662.
3D structure databases
ProteinModelPortal	Q7SFX6.
SMR	Q7SFX6. Positions 2-369.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7SFX6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000002800; EFNCRP00000002800; EFNCRG00000002797.
GeneID	3881135.
KEGG	ncr:NCU03118.
NMPDR	fig|5141.1.peg.8487.
Phylogenomic databases
eggNOG	fuNOG07631.
GeneTree	EFGT00050000005648.
OMA	QFAHCYK.
OrthoDB	EOG408RHS.
Family and domain databases
InterPro	IPR007886. AlaDH/PNT_N. IPR007698. AlaDH/PNT_NAD(H)-bd. [Graphical view]
KO	K00290.
Pfam	PF01262. AlaDh_PNT_C. 1 hit. PF05222. AlaDh_PNT_N. 1 hit. [Graphical view]
SMART	SM01002. AlaDh_PNT_C. 1 hit. SM01003. AlaDh_PNT_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYS1_NEUCR

Accession

Primary (citable) accession number: Q7SFX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	December 15, 2003
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program