Q7SFL7 (ERFB_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Palmitoyltransferase ERF2
EC=2.3.1.-

Alternative name(s):
DHHC cysteine-rich domain-containing protein ERF2
Ras protein acyltransferase

Gene names

Name:	erf-2
ORF Names:	B15B3.180, NCU02015

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora › Neurospora crassa

Protein attributes

Sequence length	680 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Palmitoyltransferase specific for Ras proteins By similarity.
Catalytic activity	Palmitoyl-CoA + protein-cysteine = S-palmitoyl protein + CoA.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Domain	The DHHC domain is required for palmitoyltransferase activity By similarity.
Post-translational modification	Autopalmitoylated By similarity.
Sequence similarities	Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily. Contains 1 DHHC-type zinc finger.
Sequence caution	The sequence CAE76200.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence EAA35644.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Acyltransferase Transferase
PTM	Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	transferase activity, transferring acyl groups Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 680	680	Palmitoyltransferase ERF2		PRO_0000212945
Regions
	Topological domain	1 – 361	361	Cytoplasmic Potential
	Transmembrane	362 – 382	21	Helical; Potential
	Topological domain	383 – 384	2	Lumenal Potential
	Transmembrane	385 – 405	21	Helical; Potential
	Topological domain	406 – 501	96	Cytoplasmic Potential
	Transmembrane	502 – 522	21	Helical; Potential
	Topological domain	523 – 543	21	Lumenal Potential
	Transmembrane	544 – 564	21	Helical; Potential
	Topological domain	565 – 680	116	Cytoplasmic Potential
	Zinc finger	457 – 507	51	DHHC-type
Experimental info
	Sequence conflict	413	1	G → GVCYSLLPF Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q7SFL7 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 18C89264D1FD64DC	FASTA	680	75,414
	10 20 30 40 50 60 MASEEGPSRS ASPTNVDNTF PQYPPRAELT VAGPPSLISS RMTDIGTEDG QGSDTTPRGG 70 80 90 100 110 120 GPSSANPNRR SGFYSDTQSR PETSGTGLSS RGPWAQSVPL RQQLTGKRGS IAGSIGSAGG 130 140 150 160 170 180 RPISAASRSH VPSLTSHGFF RPMSSQKLQA QRGAGRPPTM NRPYVITGEA GARNSVISTG 190 200 210 220 230 240 SGRIGAQIAE DAELRMPSRG TEMTEQETME RMTANTSPIN GFFPTSSVTD SVRPLQSPVD 250 260 270 280 290 300 ARNINVEMNK DYREESPTTP PRSPTRTSRS FRSSFLMPRM NESGITGSNR EIEGGEKLQS 310 320 330 340 350 360 AASSPNIPPQ SYERQRARFK RRAKRQNRAN LGKNYEYFEG NTVFCLGGRF QNTKQRPINI 370 380 390 400 410 420 ATGSLIVLPC ILFFIFSAPW IWHNISPAIP VTFAYLAYIC VSSFLHASAS DPGILPRNLH 430 440 450 460 470 480 KFPPPEMEDS PTGPPTTDWV LVHSAEASTA AMEVPIKYCK TCQLWRPPRA HHCRLCDNCV 490 500 510 520 530 540 ETQDHHCVWL NNCVGRRNYR YFFTFVSSAT VLALYLIGAC LAQILVYKNQ HHISFGHAVN 550 560 570 580 590 600 HFRVPFAMVF FGFLTFLYPA ALTGYHIFLM ARGETTREYL NSHKFPKSDR YRAFTQANWL 610 620 630 640 650 660 KNWFVVLCRP RPPTYYGFKV KYNQGDQRLG SHRRWQQPAV SDSKEGMEMQ NVSPQLPQTG 670 680 FMGPTALRNS NNGSTAEVRA

References

[1]

"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence." Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U. Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

[2]

"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842622 Genomic DNA. Translation: CAE76200.1. Sequence problems. AABX02000003 Genomic DNA. Translation: EAA35644.2. Sequence problems.
RefSeq	XP_964880.2. XM_959787.2.
UniGene	Ncr.24050.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000001222; EFNCRP00000001222; EFNCRG00000001220.
GeneID	3881029.
KEGG	ncr:NCU02015.
Phylogenomic databases
eggNOG	COG5273.
HOGENOM	HOG000194156.
OrthoDB	EOG43BQZ1.
Family and domain databases
InterPro	IPR001594. Znf_DHHC_palmitoyltrfase. [Graphical view]
Pfam	PF01529. zf-DHHC. 1 hit. [Graphical view]
PROSITE	PS50216. ZF_DHHC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERFB_NEUCR

Accession

Primary (citable) accession number: Q7SFL7
Secondary accession number(s): Q6MVU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	December 15, 2003
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program