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Q7SF84

- LIPA_NEUCR

UniProt

Q7SF84 - LIPA_NEUCR

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Protein

Lipoyl synthase, mitochondrial

Gene

NCU00565

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi142 – 1421Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi147 – 1471Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi153 – 1531Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi173 – 1731Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi177 – 1771Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi180 – 1801Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
ORF Names:NCU00565
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805: Chromosome 1, Linkage Group I

Subcellular locationi

Mitochondrion UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2929MitochondrionUniRule annotationAdd
BLAST
Chaini30 – 430401Lipoyl synthase, mitochondrialPRO_0000398275Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi5141.NCU00565.1.

Structurei

3D structure databases

ProteinModelPortaliQ7SF84.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
InParanoidiQ7SF84.
KOiK03644.
OrthoDBiEOG79KPR7.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7SF84-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASPVPIQRL QAPLRRSLAR AAALSTRSYA TIPSGPSSQP TSQESSSAAS
60 70 80 90 100
ASAPATKPRP TYFKDTTLAS LDDFIANQSS AAPLAPSEAY TLRTAEVGPA
110 120 130 140 150
GKKRTITRLP EWLKTPIPSA GANPEFAKIK ADLRGLNLHT VCEEARCPNI
160 170 180 190 200
GECWGGSNKA AATATIMLMG DTCTRGCRFC SVKTSRKPPP LDPHEPENTA
210 220 230 240 250
EALARWGLGY VVLTSVDRDD LADGGARHFA ETIRRIKQKK PTLLVEALTG
260 270 280 290 300
DFAGDLDMVK IVAESGLDVY AHNVETVENL TPYVRDRRAT FRQSLKVLEH
310 320 330 340 350
VKKVRGKEGI ITKTSIMLGL GETEEELWEA LRELRKVDVD VVTFGQYMRP
360 370 380 390 400
TKRHLAVEKY ITPDEFELWR QRALDMGFLY CASGPLVRSS YKAGEAFIEN
410 420 430
VLRKRSGEKV VSEALGQAVA AEEATSVQSS
Length:430
Mass (Da):46,902
Last modified:December 15, 2003 - v1
Checksum:i74795BCD7AD9FCCE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CM002236 Genomic DNA. Translation: EAA35490.1.
RefSeqiXP_964726.1. XM_959633.2.
UniGeneiNcr.16964.

Genome annotation databases

EnsemblFungiiEFNCRT00000000796; EFNCRP00000000796; EFNCRG00000000796.
GeneIDi3880875.
KEGGincr:NCU00565.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CM002236 Genomic DNA. Translation: EAA35490.1 .
RefSeqi XP_964726.1. XM_959633.2.
UniGenei Ncr.16964.

3D structure databases

ProteinModelPortali Q7SF84.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5141.NCU00565.1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii EFNCRT00000000796 ; EFNCRP00000000796 ; EFNCRG00000000796 .
GeneIDi 3880875.
KEGGi ncr:NCU00565.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
InParanoidi Q7SF84.
KOi K03644.
OrthoDBi EOG79KPR7.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Entry informationi

Entry nameiLIPA_NEUCR
AccessioniPrimary (citable) accession number: Q7SF84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: December 15, 2003
Last modified: October 29, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3