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Protein

3-hydroxyanthranilate 3,4-dioxygenase

Gene

bna1

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.UniRule annotation

Catalytic activityi

3-hydroxyanthranilate + O2 = 2-amino-3-carboxymuconate semialdehyde.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes quinolinate from L-kynurenine.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Kynurenine 3-monooxygenase (nic-3)
  2. Kynureninase 1 (kyn-1), Kynureninase 2 (kyn-2)
  3. 3-hydroxyanthranilate 3,4-dioxygenase (bna1)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from L-kynurenine, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44DioxygenUniRule annotation1
Metal bindingi48Iron; catalyticUniRule annotation1
Metal bindingi54Iron; catalyticUniRule annotation1
Binding sitei54SubstrateUniRule annotation1
Metal bindingi92Iron; catalyticUniRule annotation1
Binding sitei96SubstrateUniRule annotation1
Binding sitei106SubstrateUniRule annotation1
Metal bindingi121Divalent metal cationUniRule annotation1
Metal bindingi124Divalent metal cationUniRule annotation1
Metal bindingi158Divalent metal cationUniRule annotation1
Metal bindingi161Divalent metal cationUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00253; UER00330.

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxyanthranilate 3,4-dioxygenaseUniRule annotation (EC:1.13.11.6UniRule annotation)
Alternative name(s):
3-hydroxyanthranilate oxygenaseUniRule annotation
Short name:
3-HAOUniRule annotation
3-hydroxyanthranilic acid dioxygenaseUniRule annotation
Short name:
HADUniRule annotation
Biosynthesis of nicotinic acid protein 1UniRule annotation
Gene namesi
Name:bna1
ORF Names:NCU03282
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 1, Linkage Group I

Organism-specific databases

EuPathDBiFungiDB:NCU03282.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003619911 – 1803-hydroxyanthranilate 3,4-dioxygenaseAdd BLAST180

Structurei

3D structure databases

ProteinModelPortaliQ7SDX3.
SMRiQ7SDX3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-HAO family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218448.
InParanoidiQ7SDX3.
KOiK00452.
OMAiKPPVGNQ.
OrthoDBiEOG092C5CUF.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_00825. 3_HAO. 1 hit.
InterProiIPR010329. 3hydroanth_dOase.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR15497. PTHR15497. 1 hit.
PfamiPF06052. 3-HAO. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR03037. anthran_nbaC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7SDX3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSSPVNLPK WLEENSHLLK PPINNYCVYN DDFTVMIVGG PNARTDYHIN
60 70 80 90 100
QTPEWFYQYK GAMMLKVVDD GNFRDIIIRE GEMFLLPGNT PHNPVRFADT
110 120 130 140 150
VGIVLEQKRP ADTIDRMRWY CQNCKEIVHE ASFHCTDLGT QIKAAVEAFK
160 170 180
GNEQLRTCKK CGVLADWSPK PGSIQDPNVQ
Length:180
Mass (Da):20,557
Last modified:December 15, 2003 - v1
Checksum:i932C6A2C709B50DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM002236 Genomic DNA. Translation: EAA34972.1.
RefSeqiXP_964208.1. XM_959115.3.

Genome annotation databases

EnsemblFungiiEAA34972; EAA34972; NCU03282.
GeneIDi3880357.
KEGGincr:NCU03282.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM002236 Genomic DNA. Translation: EAA34972.1.
RefSeqiXP_964208.1. XM_959115.3.

3D structure databases

ProteinModelPortaliQ7SDX3.
SMRiQ7SDX3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEAA34972; EAA34972; NCU03282.
GeneIDi3880357.
KEGGincr:NCU03282.

Organism-specific databases

EuPathDBiFungiDB:NCU03282.

Phylogenomic databases

HOGENOMiHOG000218448.
InParanoidiQ7SDX3.
KOiK00452.
OMAiKPPVGNQ.
OrthoDBiEOG092C5CUF.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00330.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_00825. 3_HAO. 1 hit.
InterProiIPR010329. 3hydroanth_dOase.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR15497. PTHR15497. 1 hit.
PfamiPF06052. 3-HAO. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR03037. anthran_nbaC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry namei3HAO_NEUCR
AccessioniPrimary (citable) accession number: Q7SDX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: December 15, 2003
Last modified: November 2, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.