Reviewed, UniProtKB/Swiss-Prot Q7SB74 (DOT1_NEUCR)

Names and origin

Protein names

Recommended name:
    Histone-lysine N-methyltransferase, H3 lysine-79 specific
    EC=2.1.1.43
Alternative name(s):
    Histone H3-K79 methyltransferase
      Short name=H3-K79-HMTase

Gene names

Name:	dot-1
ORF Names:	NCU06266

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	531 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones By similarity.
Catalytic activity	S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].
Enzyme regulation	Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3 By similarity.
Subcellular location	Nucleus By similarity.
Miscellaneous	In contrast to other lysine histone methyltransferase, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.
Sequence similarities	Belongs to the DOT1 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 531	531	Histone-lysine N-methyltransferase, H3 lysine-79 specific		PRO_0000270615
Regions
	Region	377 – 379	3	S-adenosyl-L-methionine binding By similarity
	Region	437 – 438	2	S-adenosyl-L-methionine binding By similarity
	Motif	373 – 384	12	SAM-binding motif 1 By similarity
	Motif	452 – 461	10	SAM-binding motif 2 By similarity
Sites
	Binding site	355	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	401	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7SB74-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: D1ED4AFD7EBDD1BB	FASTA	531	59,294
	10 20 30 40 50 60 MSIFNQKSKF KIKTEIRTVR ETVERPKLKA STSSSSIPTT GSSQHSNVRI AGAGSSLSRA 70 80 90 100 110 120 SSTPRLNGTS HTTSTSSSAR ASLTKVTTIP TARRIQSASP IPSINGDGRS SRKRRAIKAS 130 140 150 160 170 180 SRSPISPHFS EDSDSDSNGD ENWEDNLDAR KRRKRVHDER PVDVNRKLRH PKIWKGEEAD 190 200 210 220 230 240 QVERIPGPAI IHAAKVASLE HKCKPALGLK NEEVAVRLQY PGARYPERYE LVEGKDKIDA 250 260 270 280 290 300 VKDIEKVVQL AASTYLTDEE GKIFLDHQTG ILRKLVKSKN RSDGPGFKAA LREYNDEFLK 310 320 330 340 350 360 LMRKGVIVRN LDKMHGIPRE LVDFILDQVY DRTVAPRVEL LAKYQNGSDN VYGELNHPFI 370 380 390 400 410 420 SDILERTNLT SDKVFVDLGS GVGNVTLQAA LEIGCESWGC EMMENACNLA EAQYDEFLAR 430 440 450 460 470 480 CRLWGVKPGK VRLERGDFRK NEKILEVLKR ADVVLVNNQA FTSQLNDNLV SMFLDLKIGC 490 500 510 520 530 KIISLKTFVH DNKNAENDVA TSILDVEHLT YPEGYVSWTG ASGTYCISTR R

References

[1]

"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AABX02000025 Genomic DNA. Translation: EAA33634.1.
RefSeq	XP_962870.1.
3D structure databases
HSSP	HSSP built from PDB template 1U2Z based on UniProtKB Q04089.
SMR	Q7SB74. Positions 216-530.
ModBase	Search...
Genome annotation databases
GeneID	3879023.
KEGG	ncr:NCU06266.
NMPDR	fig|5141.1.peg.4054.
Phylogenomic databases
eggNOG	fuNOG04919.
OrthoDB	EOG9FBK98.
PhylomeDB	Q7SB74.
Enzyme and pathway databases
BRENDA	2.1.1.43. 266.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

DOT1_NEUCR

Accession

Primary (citable) accession number: Q7SB74

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	December 15, 2003
Last modified:	February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)