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Protein

Histone-lysine N-methyltransferase, H3 lysine-79 specific

Gene

dot-1

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].PROSITE-ProRule annotation

Enzyme regulationi

Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei401S-adenosyl-L-methioninePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Methyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase, H3 lysine-79 specific (EC:2.1.1.43)
Alternative name(s):
Histone H3-K79 methyltransferase
Short name:
H3-K79-HMTase
Gene namesi
Name:dot-1
ORF Names:NCU06266
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 3, Linkage Group III

Organism-specific databases

EuPathDBiFungiDB:NCU06266.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002706151 – 531Histone-lysine N-methyltransferase, H3 lysine-79 specificAdd BLAST531

Structurei

3D structure databases

ProteinModelPortaliQ7SB74.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini214 – 531DOT1PROSITE-ProRule annotationAdd BLAST318

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni352 – 355S-adenosyl-L-methionine bindingPROSITE-ProRule annotation4
Regioni375 – 384S-adenosyl-L-methionine bindingPROSITE-ProRule annotation10
Regioni437 – 438S-adenosyl-L-methionine bindingPROSITE-ProRule annotation2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.PROSITE-ProRule annotation
Contains 1 DOT1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000176257.
InParanoidiQ7SB74.
KOiK11427.
OMAiVEHLTYP.
OrthoDBiEOG092C18VU.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR021162. Dot1.
IPR025789. DOT1_dom.
IPR030445. H3-K79_meTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR21451. PTHR21451. 1 hit.
PfamiPF08123. DOT1. 1 hit.
[Graphical view]
PIRSFiPIRSF017570. Histone_H3-K79_MeTrfase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51569. DOT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7SB74-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIFNQKSKF KIKTEIRTVR ETVERPKLKA STSSSSIPTT GSSQHSNVRI
60 70 80 90 100
AGAGSSLSRA SSTPRLNGTS HTTSTSSSAR ASLTKVTTIP TARRIQSASP
110 120 130 140 150
IPSINGDGRS SRKRRAIKAS SRSPISPHFS EDSDSDSNGD ENWEDNLDAR
160 170 180 190 200
KRRKRVHDER PVDVNRKLRH PKIWKGEEAD QVERIPGPAI IHAAKVASLE
210 220 230 240 250
HKCKPALGLK NEEVAVRLQY PGARYPERYE LVEGKDKIDA VKDIEKVVQL
260 270 280 290 300
AASTYLTDEE GKIFLDHQTG ILRKLVKSKN RSDGPGFKAA LREYNDEFLK
310 320 330 340 350
LMRKGVIVRN LDKMHGIPRE LVDFILDQVY DRTVAPRVEL LAKYQNGSDN
360 370 380 390 400
VYGELNHPFI SDILERTNLT SDKVFVDLGS GVGNVTLQAA LEIGCESWGC
410 420 430 440 450
EMMENACNLA EAQYDEFLAR CRLWGVKPGK VRLERGDFRK NEKILEVLKR
460 470 480 490 500
ADVVLVNNQA FTSQLNDNLV SMFLDLKIGC KIISLKTFVH DNKNAENDVA
510 520 530
TSILDVEHLT YPEGYVSWTG ASGTYCISTR R
Length:531
Mass (Da):59,294
Last modified:December 15, 2003 - v1
Checksum:iD1ED4AFD7EBDD1BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM002238 Genomic DNA. Translation: EAA33634.1.
RefSeqiXP_962870.1. XM_957777.2.

Genome annotation databases

EnsemblFungiiEAA33634; EAA33634; NCU06266.
GeneIDi3879023.
KEGGincr:NCU06266.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM002238 Genomic DNA. Translation: EAA33634.1.
RefSeqiXP_962870.1. XM_957777.2.

3D structure databases

ProteinModelPortaliQ7SB74.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEAA33634; EAA33634; NCU06266.
GeneIDi3879023.
KEGGincr:NCU06266.

Organism-specific databases

EuPathDBiFungiDB:NCU06266.

Phylogenomic databases

HOGENOMiHOG000176257.
InParanoidiQ7SB74.
KOiK11427.
OMAiVEHLTYP.
OrthoDBiEOG092C18VU.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR021162. Dot1.
IPR025789. DOT1_dom.
IPR030445. H3-K79_meTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR21451. PTHR21451. 1 hit.
PfamiPF08123. DOT1. 1 hit.
[Graphical view]
PIRSFiPIRSF017570. Histone_H3-K79_MeTrfase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51569. DOT1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDOT1_NEUCR
AccessioniPrimary (citable) accession number: Q7SB74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: December 15, 2003
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.