ID   MCE1_NEUCR              Reviewed;         402 AA.
AC   Q7SB53;
DT   27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   16-JUN-2009, entry version 41.
DE   RecName: Full=mRNA-capping enzyme subunit alpha;
DE   AltName: Full=mRNA guanylyltransferase;
DE            EC=2.7.7.50;
DE   AltName: Full=GTP--RNA guanylyltransferase;
DE            Short=GTase;
GN   ORFNames=NCU06260;
OS   Neurospora crassa.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
OC   Sordariomycetes; Sordariomycetidae; Sordariales; Sordariaceae;
OC   Neurospora.
OX   NCBI_TaxID=5141;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   MEDLINE=22598136; PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A.,
RA   Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L.,
RA   Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C.,
RA   Marcotte E., Greenberg D., Roy A., Foley K., Naylor J.,
RA   Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M.,
RA   Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S.,
RA   Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C.,
RA   Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M.,
RA   Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Second step of mRNA capping. Transfer of the GMP moiety
CC       of GTP to the 5'-end of RNA via an enzyme-GMP covalent reaction
CC       intermediate (By similarity).
CC   -!- CATALYTIC ACTIVITY: GTP + (5')pp-Pur-mRNA = diphosphate +
CC       G(5')ppp-Pur-mRNA.
CC   -!- SUBUNIT: The mRNA-capping enzyme is composed of two separate
CC       chains alpha and beta, respectively a mRNA guanylyltransferase and
CC       an RNA 5'-triphosphatase (By similarity).
CC   -!- SUBCELLULAR LOCATION: Nucleus (By similarity).
CC   -!- SIMILARITY: Belongs to the eukaryotic GTase family.
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DR   EMBL; AABX02000025; EAA33628.1; -; Genomic_DNA.
DR   RefSeq; XP_962864.1; -.
DR   GeneID; 3879017; -.
DR   KEGG; ncr:NCU06260; -.
DR   NMPDR; fig|5141.1.peg.4048; -.
DR   BRENDA; 2.7.7.50; 266.
DR   GO; GO:0031533; C:mRNA capping enzyme complex; IEA:InterPro.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004484; F:mRNA guanylyltransferase activity; IEA:EC.
DR   GO; GO:0005515; F:protein binding; IEA:InterPro.
DR   GO; GO:0006370; P:mRNA capping; IEA:UniProtKB-KW.
DR   InterPro; IPR001339; mRNA_cap_enzyme.
DR   InterPro; IPR013846; mRNA_cap_enzyme_C.
DR   InterPro; IPR017075; mRNA_capping_enz_asu.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   Gene3D; G3DSA:2.40.50.140; OB_NA_bd_sub; 1.
DR   Pfam; PF03919; mRNA_cap_C; 1.
DR   Pfam; PF01331; mRNA_cap_enzyme; 1.
DR   PIRSF; PIRSF036959; mRNA_cap_alpha; 1.
PE   3: Inferred from homology;
KW   Complete proteome; GTP-binding; mRNA capping; mRNA processing;
KW   Nucleotide-binding; Nucleotidyltransferase; Nucleus; Transferase.
FT   CHAIN         1    402       mRNA-capping enzyme subunit alpha.
FT                                /FTId=PRO_0000210103.
FT   ACT_SITE     66     66       N6-GMP-lysine intermediate (By
FT                                similarity).
SQ   SEQUENCE   402 AA;  46572 MW;  9A3862BF446C0F60 CRC64;
     MEQPGPITDI AKPGIKAPRD LAISLREEVA RILGRSSIGF PGAQPVSFAR KHLEELRRED
     YYVCEKSDGI RYLLYLTVDE EGQEVQYLID RKNDYWFLPR NSMHFPMPND IQAFHRGTII
     DGELVMDTVP GTNGRKEPRF LVFDLLALDD KAELLNKPLD KRLGYFSAYI YEPYKKLLQQ
     FPQEIPFMAF KVEMKRMELS YGIETMFREV IPALKHDSDG LIFTCRTTPY HFGTDPHILK
     WKAPHENTLD FRMRLNFPLV QATEAELDEG FPEQFTDYDS VPQAELYVFC GNDGPGGSKY
     ELFPDPLYIA EDEWETLKAL GDPLQDRVVE CCLDAENRWR LFRFRDDKNE ANHTSTVSSV
     MASIRDGVSD QELLSAATAI KESWKIRAQK RKEQQQQQQP KH
//