Reviewed, UniProtKB/Swiss-Prot Q7SB53 (MCE1_NEUCR)

Names and origin

Protein names

Recommended name:
    mRNA-capping enzyme subunit alpha
Alternative name(s):
    mRNA guanylyltransferase
    EC=2.7.7.50
    GTP--RNA guanylyltransferase
      Short name=GTase

Gene names

ORF Names:

NCU06260

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Second step of mRNA capping. Transfer of the GMP moiety of GTP to the 5'-end of RNA via an enzyme-GMP covalent reaction intermediate By similarity.
Catalytic activity	GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
Subunit structure	The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an RNA 5'-triphosphatase By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the eukaryotic GTase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Nucleus
Ligand	GTP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mRNA capping enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA guanylyltransferase activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 402	402	mRNA-capping enzyme subunit alpha		PRO_0000210103
Sites
	Active site	66	1	N6-GMP-lysine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7SB53-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 9A3862BF446C0F60	FASTA	402	46,572
	10 20 30 40 50 60 MEQPGPITDI AKPGIKAPRD LAISLREEVA RILGRSSIGF PGAQPVSFAR KHLEELRRED 70 80 90 100 110 120 YYVCEKSDGI RYLLYLTVDE EGQEVQYLID RKNDYWFLPR NSMHFPMPND IQAFHRGTII 130 140 150 160 170 180 DGELVMDTVP GTNGRKEPRF LVFDLLALDD KAELLNKPLD KRLGYFSAYI YEPYKKLLQQ 190 200 210 220 230 240 FPQEIPFMAF KVEMKRMELS YGIETMFREV IPALKHDSDG LIFTCRTTPY HFGTDPHILK 250 260 270 280 290 300 WKAPHENTLD FRMRLNFPLV QATEAELDEG FPEQFTDYDS VPQAELYVFC GNDGPGGSKY 310 320 330 340 350 360 ELFPDPLYIA EDEWETLKAL GDPLQDRVVE CCLDAENRWR LFRFRDDKNE ANHTSTVSSV 370 380 390 400 MASIRDGVSD QELLSAATAI KESWKIRAQK RKEQQQQQQP KH

References

[1]

"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AABX02000025 Genomic DNA. Translation: EAA33628.1.
RefSeq	XP_962864.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3879017.
KEGG	ncr:NCU06260.
NMPDR	fig|5141.1.peg.4048.
Enzyme and pathway databases
BRENDA	2.7.7.50. 266.
Family and domain databases
InterPro	IPR001339. mRNA_cap_enzyme. IPR013846. mRNA_cap_enzyme_C. IPR017075. mRNA_capping_enz_asu. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF03919. mRNA_cap_C. 1 hit. PF01331. mRNA_cap_enzyme. 1 hit. [Graphical view]
PIRSF	PIRSF036959. mRNA_cap_alpha. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCE1_NEUCR

Accession

Primary (citable) accession number: Q7SB53

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	December 15, 2003
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)