Reviewed, UniProtKB/Swiss-Prot Q7SB49 (DNLI4_NEUCR)

Names and origin

Protein names

Recommended name:
    DNA ligase 4
    EC=6.5.1.1
Alternative name(s):
    DNA ligase IV
    Polydeoxyribonucleotide synthase [ATP] 4
    DNA repair and recombination protein mus-53

Gene names

Name:	lig-4
Synonyms:	mus-53
ORF Names:	NCU06264

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	1046 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining. Ref.1
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Cofactor	Magnesium By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family. Contains 2 BRCT domains.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair DNA replication
Cellular component	Nucleus
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1046	1046	DNA ligase 4		PRO_0000278384
Regions
	Domain	738 – 836	99	BRCT 1
	Domain	932 – 1045	114	BRCT 2
Sites
	Active site	327	1	N6-AMP-lysine intermediate By similarity
	Metal binding	394	1	Magnesium 1 Potential
	Metal binding	494	1	Magnesium 2 Potential
	Binding site	325	1	ATP By similarity
	Binding site	332	1	ATP By similarity
	Binding site	354	1	ATP By similarity
	Binding site	499	1	ATP By similarity
	Binding site	510	1	ATP By similarity
	Binding site	516	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7SB49-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 072B0B5B806A3146	FASTA	1,046	118,910
	10 20 30 40 50 60 MNTNRRSRSP DEEALEEDQH QYGAGTLSLE ELDEQPRNHS KTFPFSDLFR TLFNPLIDCK 70 80 90 100 110 120 PSTSGGTVRG PKPGRGGHFS KVSYHEQRRH IIERFMSRWR SEVGNDFYPA MRLILPDKDR 130 140 150 160 170 180 DRGVYGLKEN TIGKLLVKVM KIDRNSEDGY NLMHWKLPGG QSGVSRSVGD FAGRCLEVVS 190 200 210 220 230 240 KRAMRAQPGD LTIADVNVLL DRLAAASGEA EQLPIFEEFY RQMNAEEMMW LVRIILKDMR 250 260 270 280 290 300 VGATERTFLN LWHPDAEALF SVSSSLRRVC WELFDPEFRL EQQETGIKLM QCFQPQLAQF 310 320 330 340 350 360 QMTTTWEKLV KNLGVTEENP EFWIEEKLDG ERMQMHMIED DTVPGGFRFA FWSRKAKDYT 370 380 390 400 410 420 YLYGESLGDE QSALTRHLHK AFDDGVRNLI LDGEMITWDI DIDKMVPFGT LKTAALEQQK 430 440 450 460 470 480 NPSKAGPRPL YRVFDILLLN DKPLTEYTLN DRRRALERAV VGVHRRLEIL PFERATSPDA 490 500 510 520 530 540 IEPLLRRVVA EASEGLVLKN PRSRYSLNSR NNDWIKVKPE YMSDFGESLD CVVVGGYFGS 550 560 570 580 590 600 GRRGGTLSSF LCGVRVSQNF IKSGNASAEK CLSFVKVGGG FKAEDYAEIR HHTEGKWQDW 610 620 630 640 650 660 DPSSPPTEYI ELGGGEKLQY EKPDVWIRPS DSVVISVKAA SITQSDQFAM GWTLRFPRFR 670 680 690 700 710 720 KLRLDRAWDS ALDMDEFEVL RSKIKDQEQE RKKMEMENRK RKPATKRARK DLVIAGMSDP 730 740 750 760 770 780 SSSSAATPVI APKETREASK RLFEGLDFCV LSDSLKPNKM TKPALEKLIK DHGGRIHQQV 790 800 810 820 830 840 MDHSGQGKII IPIADKNVIK VASLRKANPE MDIIRPKWIF DCLVQPMPFT KQKENKKGYL 850 860 870 880 890 900 LPFEPTHLFH SGSEETSEEA EQAVDKFGDS YAGDLADINE LKAIMEGMES DDYVSDSDWD 910 920 930 940 950 960 SDSGRGRGGG DGFDMNHFLD HLEEQGTSLD DLRSFMFRRC RVFFALPSAG NGDGAAESKA 970 980 990 1000 1010 1020 LRLKNYIRFG NGKVVDELET ATHVVVVTAP LGESSKKEER EIAAELRYKI SLREMGSPMP 1030 1040 RIVKGEWVED SWKEGTVVDE EEYVAG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nonhomologous chromosomal integration of foreign DNA is completely dependent on MUS-53 (human Lig4 homolog) in Neurospora." Ishibashi K., Suzuki K., Ando Y., Takakura C., Inoue H. Proc. Natl. Acad. Sci. U.S.A. 103:14871-14876(2006) [PubMed: 17003123] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB261106 Genomic DNA. Translation: BAF34364.1. AABX02000025 Genomic DNA. Translation: EAA33632.1.
RefSeq	XP_962868.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3879007.
KEGG	ncr:NCU06264.
NMPDR	fig|5141.1.peg.4052.
Enzyme and pathway databases
BRENDA	6.5.1.1. 266.
Family and domain databases
InterPro	IPR001357. BRCT. IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF00533. BRCT. 1 hit. PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 2 hits. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS50172. BRCT. 2 hits. PS00697. DNA_LIGASE_A1. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI4_NEUCR

Accession

Primary (citable) accession number: Q7SB49

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	December 15, 2003
Last modified:	June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)