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Q7SB49

- DNLI4_NEUCR

UniProt

Q7SB49 - DNLI4_NEUCR

Protein

DNA ligase 4

Gene

mus-53

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 2 (19 Mar 2014)
      Previous versions | rss
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    Functioni

    Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining.1 Publication

    Catalytic activityi

    ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).PROSITE-ProRule annotation

    Cofactori

    Magnesium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei329 – 3291ATPBy similarity
    Active sitei331 – 3311N6-AMP-lysine intermediatePROSITE-ProRule annotation
    Binding sitei336 – 3361ATPBy similarity
    Binding sitei358 – 3581ATPBy similarity
    Metal bindingi398 – 3981Magnesium 1Sequence Analysis
    Metal bindingi498 – 4981Magnesium 2Sequence Analysis
    Binding sitei503 – 5031ATPBy similarity
    Binding sitei514 – 5141ATPBy similarity
    Binding sitei520 – 5201ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: InterPro
    3. DNA ligase (ATP) activity Source: RefGenome
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA ligation Source: RefGenome
    2. DNA ligation involved in DNA repair Source: InterPro
    3. DNA recombination Source: UniProtKB-KW
    4. double-strand break repair via nonhomologous end joining Source: RefGenome
    5. lagging strand elongation Source: RefGenome
    6. nucleotide-excision repair Source: RefGenome

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair, DNA replication

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA ligase 4 (EC:6.5.1.1)
    Alternative name(s):
    DNA ligase IV
    Mutagen-sensitive protein 53
    Polydeoxyribonucleotide synthase [ATP] 4
    Gene namesi
    Name:mus-53
    Synonyms:lig4
    ORF Names:NCU06264
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Chromosome 3, Linkage Group III

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. chromosome Source: RefGenome
    2. DNA ligase IV complex Source: RefGenome

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10501050DNA ligase 4PRO_0000278384Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi5141.NCU06264.1.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini742 – 84099BRCT 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini936 – 1049114BRCT 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ATP-dependent DNA ligase family.Curated
    Contains 2 BRCT domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1793.
    HOGENOMiHOG000176213.
    KOiK10777.
    OMAiHMCPSTK.
    OrthoDBiEOG7QC84G.

    Family and domain databases

    Gene3Di1.10.3260.10. 1 hit.
    2.40.50.140. 1 hit.
    3.40.50.10190. 2 hits.
    InterProiIPR001357. BRCT_dom.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view]
    PfamiPF00533. BRCT. 1 hit.
    PF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view]
    SMARTiSM00292. BRCT. 2 hits.
    [Graphical view]
    SUPFAMiSSF117018. SSF117018. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF52113. SSF52113. 2 hits.
    TIGRFAMsiTIGR00574. dnl1. 1 hit.
    PROSITEiPS50172. BRCT. 2 hits.
    PS00697. DNA_LIGASE_A1. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7SB49-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNTNRRSRSP DEEALEEDQH QYGAGTLSLE ELDEHFPNRP RNHSKTFPFS     50
    DLFRTLFNPL IDCKPSTSGG TVRGPKPGRG GHFSKVSYHE QRRHIIERFM 100
    SRWRSEVGND FYPAMRLILP DKDRDRGVYG LKENTIGKLL VKVMKIDRNS 150
    EDGYNLMHWK LPGGQSGVSR SVGDFAGRCL EVVSKRAMRA QPGDLTIADV 200
    NVLLDRLAAA SGEAEQLPIF EEFYRQMNAE EMMWLVRIIL KDMRVGATER 250
    TFLNLWHPDA EALFSVSSSL RRVCWELFDP EFRLEQQETG IKLMQCFQPQ 300
    LAQFQMTTTW EKLVKNLGVT EENPEFWIEE KLDGERMQMH MIEDDTVPGG 350
    FRFAFWSRKA KDYTYLYGES LGDEQSALTR HLHKAFDDGV RNLILDGEMI 400
    TWDIDIDKMV PFGTLKTAAL EQQKNPSKAG PRPLYRVFDI LLLNDKPLTE 450
    YTLNDRRRAL ERAVVGVHRR LEILPFERAT SPDAIEPLLR RVVAEASEGL 500
    VLKNPRSRYS LNSRNNDWIK VKPEYMSDFG ESLDCVVVGG YFGSGRRGGT 550
    LSSFLCGVRV SQNFIKSGNA SAEKCLSFVK VGGGFKAEDY AEIRHHTEGK 600
    WQDWDPSSPP TEYIELGGGE KLQYEKPDVW IRPSDSVVIS VKAASITQSD 650
    QFAMGWTLRF PRFRKLRLDR AWDSALDMDE FEVLRSKIKD QEQERKKMEM 700
    ENRKRKPATK RARKDLVIAG MSDPSSSSAA TPVIAPKETR EASKRLFEGL 750
    DFCVLSDSLK PNKMTKPALE KLIKDHGGRI HQQVMDHSGQ GKIIIPIADK 800
    NVIKVASLRK ANPEMDIIRP KWIFDCLVQP MPFTKQKENK KGYLLPFEPT 850
    HLFHSGSEET SEEAEQAVDK FGDSYAGDLA DINELKAIME GMESDDYVSD 900
    SDWDSDSGRG RGGGDGFDMN HFLDHLEEQG TSLDDLRSFM FRRCRVFFAL 950
    PSAGNGDGAA ESKALRLKNY IRFGNGKVVD ELETATHVVV VTAPLGESSK 1000
    KEEREIAAEL RYKISLREMG SPMPRIVKGE WVEDSWKEGT VVDEEEYVAG 1050
    Length:1,050
    Mass (Da):119,433
    Last modified:March 19, 2014 - v2
    Checksum:iCDA8E04CF37AFA99
    GO

    Sequence cautioni

    The sequence BAF34364.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB261106 Genomic DNA. Translation: BAF34364.1. Sequence problems.
    CM002238 Genomic DNA. Translation: EAA33632.2.
    RefSeqiXP_962868.1. XM_957775.1.
    UniGeneiNcr.24770.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000006055; EFNCRP00000006048; EFNCRG00000006045.
    GeneIDi3879007.
    KEGGincr:NCU06264.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB261106 Genomic DNA. Translation: BAF34364.1 . Sequence problems.
    CM002238 Genomic DNA. Translation: EAA33632.2 .
    RefSeqi XP_962868.1. XM_957775.1.
    UniGenei Ncr.24770.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5141.NCU06264.1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFNCRT00000006055 ; EFNCRP00000006048 ; EFNCRG00000006045 .
    GeneIDi 3879007.
    KEGGi ncr:NCU06264.

    Phylogenomic databases

    eggNOGi COG1793.
    HOGENOMi HOG000176213.
    KOi K10777.
    OMAi HMCPSTK.
    OrthoDBi EOG7QC84G.

    Family and domain databases

    Gene3Di 1.10.3260.10. 1 hit.
    2.40.50.140. 1 hit.
    3.40.50.10190. 2 hits.
    InterProi IPR001357. BRCT_dom.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view ]
    Pfami PF00533. BRCT. 1 hit.
    PF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view ]
    SMARTi SM00292. BRCT. 2 hits.
    [Graphical view ]
    SUPFAMi SSF117018. SSF117018. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF52113. SSF52113. 2 hits.
    TIGRFAMsi TIGR00574. dnl1. 1 hit.
    PROSITEi PS50172. BRCT. 2 hits.
    PS00697. DNA_LIGASE_A1. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nonhomologous chromosomal integration of foreign DNA is completely dependent on MUS-53 (human Lig4 homolog) in Neurospora."
      Ishibashi K., Suzuki K., Ando Y., Takakura C., Inoue H.
      Proc. Natl. Acad. Sci. U.S.A. 103:14871-14876(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    2. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

    Entry informationi

    Entry nameiDNLI4_NEUCR
    AccessioniPrimary (citable) accession number: Q7SB49
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 20, 2007
    Last sequence update: March 19, 2014
    Last modified: October 1, 2014
    This is version 88 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3