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Q7SB49

- DNLI4_NEUCR

UniProt

Q7SB49 - DNLI4_NEUCR

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Protein

DNA ligase 4

Gene

mus-53

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining.1 Publication

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).PROSITE-ProRule annotation

Cofactori

Magnesium.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei329 – 3291ATPBy similarity
Active sitei331 – 3311N6-AMP-lysine intermediatePROSITE-ProRule annotation
Binding sitei336 – 3361ATPBy similarity
Binding sitei358 – 3581ATPBy similarity
Metal bindingi398 – 3981Magnesium 1Sequence Analysis
Metal bindingi498 – 4981Magnesium 2Sequence Analysis
Binding sitei503 – 5031ATPBy similarity
Binding sitei514 – 5141ATPBy similarity
Binding sitei520 – 5201ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: RefGenome
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA biosynthetic process Source: InterPro
  2. DNA ligation Source: RefGenome
  3. DNA ligation involved in DNA repair Source: InterPro
  4. DNA recombination Source: UniProtKB-KW
  5. double-strand break repair via nonhomologous end joining Source: RefGenome
  6. lagging strand elongation Source: RefGenome
  7. nucleotide-excision repair Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 4 (EC:6.5.1.1)
Alternative name(s):
DNA ligase IV
Mutagen-sensitive protein 53
Polydeoxyribonucleotide synthase [ATP] 4
Gene namesi
Name:mus-53
Synonyms:lig4
ORF Names:NCU06264
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805: Chromosome 3, Linkage Group III

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. chromosome Source: RefGenome
  2. DNA ligase IV complex Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10501050DNA ligase 4PRO_0000278384Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi5141.NCU06264.1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini742 – 84099BRCT 1PROSITE-ProRule annotationAdd
BLAST
Domaini936 – 1049114BRCT 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.Curated
Contains 2 BRCT domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1793.
HOGENOMiHOG000176213.
InParanoidiQ7SB49.
KOiK10777.
OMAiHMCPSTK.
OrthoDBiEOG7QC84G.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
3.40.50.10190. 2 hits.
InterProiIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR029710. LIG4.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PANTHERiPTHR10459:SF7. PTHR10459:SF7. 1 hit.
PfamiPF00533. BRCT. 1 hit.
PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 2 hits.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 2 hits.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS50172. BRCT. 2 hits.
PS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7SB49-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNTNRRSRSP DEEALEEDQH QYGAGTLSLE ELDEHFPNRP RNHSKTFPFS
60 70 80 90 100
DLFRTLFNPL IDCKPSTSGG TVRGPKPGRG GHFSKVSYHE QRRHIIERFM
110 120 130 140 150
SRWRSEVGND FYPAMRLILP DKDRDRGVYG LKENTIGKLL VKVMKIDRNS
160 170 180 190 200
EDGYNLMHWK LPGGQSGVSR SVGDFAGRCL EVVSKRAMRA QPGDLTIADV
210 220 230 240 250
NVLLDRLAAA SGEAEQLPIF EEFYRQMNAE EMMWLVRIIL KDMRVGATER
260 270 280 290 300
TFLNLWHPDA EALFSVSSSL RRVCWELFDP EFRLEQQETG IKLMQCFQPQ
310 320 330 340 350
LAQFQMTTTW EKLVKNLGVT EENPEFWIEE KLDGERMQMH MIEDDTVPGG
360 370 380 390 400
FRFAFWSRKA KDYTYLYGES LGDEQSALTR HLHKAFDDGV RNLILDGEMI
410 420 430 440 450
TWDIDIDKMV PFGTLKTAAL EQQKNPSKAG PRPLYRVFDI LLLNDKPLTE
460 470 480 490 500
YTLNDRRRAL ERAVVGVHRR LEILPFERAT SPDAIEPLLR RVVAEASEGL
510 520 530 540 550
VLKNPRSRYS LNSRNNDWIK VKPEYMSDFG ESLDCVVVGG YFGSGRRGGT
560 570 580 590 600
LSSFLCGVRV SQNFIKSGNA SAEKCLSFVK VGGGFKAEDY AEIRHHTEGK
610 620 630 640 650
WQDWDPSSPP TEYIELGGGE KLQYEKPDVW IRPSDSVVIS VKAASITQSD
660 670 680 690 700
QFAMGWTLRF PRFRKLRLDR AWDSALDMDE FEVLRSKIKD QEQERKKMEM
710 720 730 740 750
ENRKRKPATK RARKDLVIAG MSDPSSSSAA TPVIAPKETR EASKRLFEGL
760 770 780 790 800
DFCVLSDSLK PNKMTKPALE KLIKDHGGRI HQQVMDHSGQ GKIIIPIADK
810 820 830 840 850
NVIKVASLRK ANPEMDIIRP KWIFDCLVQP MPFTKQKENK KGYLLPFEPT
860 870 880 890 900
HLFHSGSEET SEEAEQAVDK FGDSYAGDLA DINELKAIME GMESDDYVSD
910 920 930 940 950
SDWDSDSGRG RGGGDGFDMN HFLDHLEEQG TSLDDLRSFM FRRCRVFFAL
960 970 980 990 1000
PSAGNGDGAA ESKALRLKNY IRFGNGKVVD ELETATHVVV VTAPLGESSK
1010 1020 1030 1040 1050
KEEREIAAEL RYKISLREMG SPMPRIVKGE WVEDSWKEGT VVDEEEYVAG
Length:1,050
Mass (Da):119,433
Last modified:March 19, 2014 - v2
Checksum:iCDA8E04CF37AFA99
GO

Sequence cautioni

The sequence BAF34364.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB261106 Genomic DNA. Translation: BAF34364.1. Sequence problems.
CM002238 Genomic DNA. Translation: EAA33632.2.
RefSeqiXP_962868.1. XM_957775.1.
UniGeneiNcr.24770.

Genome annotation databases

EnsemblFungiiEFNCRT00000006055; EFNCRP00000006048; EFNCRG00000006045.
GeneIDi3879007.
KEGGincr:NCU06264.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB261106 Genomic DNA. Translation: BAF34364.1 . Sequence problems.
CM002238 Genomic DNA. Translation: EAA33632.2 .
RefSeqi XP_962868.1. XM_957775.1.
UniGenei Ncr.24770.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5141.NCU06264.1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii EFNCRT00000006055 ; EFNCRP00000006048 ; EFNCRG00000006045 .
GeneIDi 3879007.
KEGGi ncr:NCU06264.

Phylogenomic databases

eggNOGi COG1793.
HOGENOMi HOG000176213.
InParanoidi Q7SB49.
KOi K10777.
OMAi HMCPSTK.
OrthoDBi EOG7QC84G.

Family and domain databases

Gene3Di 1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
3.40.50.10190. 2 hits.
InterProi IPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR029710. LIG4.
IPR012340. NA-bd_OB-fold.
[Graphical view ]
PANTHERi PTHR10459:SF7. PTHR10459:SF7. 1 hit.
Pfami PF00533. BRCT. 1 hit.
PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view ]
SMARTi SM00292. BRCT. 2 hits.
[Graphical view ]
SUPFAMi SSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 2 hits.
TIGRFAMsi TIGR00574. dnl1. 1 hit.
PROSITEi PS50172. BRCT. 2 hits.
PS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nonhomologous chromosomal integration of foreign DNA is completely dependent on MUS-53 (human Lig4 homolog) in Neurospora."
    Ishibashi K., Suzuki K., Ando Y., Takakura C., Inoue H.
    Proc. Natl. Acad. Sci. U.S.A. 103:14871-14876(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Entry informationi

Entry nameiDNLI4_NEUCR
AccessioniPrimary (citable) accession number: Q7SB49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: March 19, 2014
Last modified: October 29, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3