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Q7SB49

- DNLI4_NEUCR

UniProt

Q7SB49 - DNLI4_NEUCR

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Protein
DNA ligase 4
Gene
mus-53, lig4, NCU06264
Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining.1 Publication

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactori

Magnesium By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei329 – 3291ATP By similarity
Active sitei331 – 3311N6-AMP-lysine intermediate By similarity
Binding sitei336 – 3361ATP By similarity
Binding sitei358 – 3581ATP By similarity
Metal bindingi398 – 3981Magnesium 1 Reviewed prediction
Metal bindingi498 – 4981Magnesium 2 Reviewed prediction
Binding sitei503 – 5031ATP By similarity
Binding sitei514 – 5141ATP By similarity
Binding sitei520 – 5201ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: RefGenome
  4. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. DNA ligation Source: RefGenome
  2. DNA ligation involved in DNA repair Source: InterPro
  3. DNA recombination Source: UniProtKB-KW
  4. double-strand break repair via nonhomologous end joining Source: RefGenome
  5. lagging strand elongation Source: RefGenome
  6. nucleotide-excision repair Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 4 (EC:6.5.1.1)
Alternative name(s):
DNA ligase IV
Mutagen-sensitive protein 53
Polydeoxyribonucleotide synthase [ATP] 4
Gene namesi
Name:mus-53
Synonyms:lig4
ORF Names:NCU06264
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805: Chromosome 3, Linkage Group III

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. DNA ligase IV complex Source: RefGenome
  2. chromosome Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10501050DNA ligase 4
PRO_0000278384Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi5141.NCU06264.1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini742 – 84099BRCT 1
Add
BLAST
Domaini936 – 1049114BRCT 2
Add
BLAST

Sequence similaritiesi

Contains 2 BRCT domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1793.
HOGENOMiHOG000176213.
KOiK10777.
OMAiHMCPSTK.
OrthoDBiEOG7QC84G.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
3.40.50.10190. 2 hits.
InterProiIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 2 hits.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 2 hits.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS50172. BRCT. 2 hits.
PS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7SB49-1 [UniParc]FASTAAdd to Basket

« Hide

MNTNRRSRSP DEEALEEDQH QYGAGTLSLE ELDEHFPNRP RNHSKTFPFS     50
DLFRTLFNPL IDCKPSTSGG TVRGPKPGRG GHFSKVSYHE QRRHIIERFM 100
SRWRSEVGND FYPAMRLILP DKDRDRGVYG LKENTIGKLL VKVMKIDRNS 150
EDGYNLMHWK LPGGQSGVSR SVGDFAGRCL EVVSKRAMRA QPGDLTIADV 200
NVLLDRLAAA SGEAEQLPIF EEFYRQMNAE EMMWLVRIIL KDMRVGATER 250
TFLNLWHPDA EALFSVSSSL RRVCWELFDP EFRLEQQETG IKLMQCFQPQ 300
LAQFQMTTTW EKLVKNLGVT EENPEFWIEE KLDGERMQMH MIEDDTVPGG 350
FRFAFWSRKA KDYTYLYGES LGDEQSALTR HLHKAFDDGV RNLILDGEMI 400
TWDIDIDKMV PFGTLKTAAL EQQKNPSKAG PRPLYRVFDI LLLNDKPLTE 450
YTLNDRRRAL ERAVVGVHRR LEILPFERAT SPDAIEPLLR RVVAEASEGL 500
VLKNPRSRYS LNSRNNDWIK VKPEYMSDFG ESLDCVVVGG YFGSGRRGGT 550
LSSFLCGVRV SQNFIKSGNA SAEKCLSFVK VGGGFKAEDY AEIRHHTEGK 600
WQDWDPSSPP TEYIELGGGE KLQYEKPDVW IRPSDSVVIS VKAASITQSD 650
QFAMGWTLRF PRFRKLRLDR AWDSALDMDE FEVLRSKIKD QEQERKKMEM 700
ENRKRKPATK RARKDLVIAG MSDPSSSSAA TPVIAPKETR EASKRLFEGL 750
DFCVLSDSLK PNKMTKPALE KLIKDHGGRI HQQVMDHSGQ GKIIIPIADK 800
NVIKVASLRK ANPEMDIIRP KWIFDCLVQP MPFTKQKENK KGYLLPFEPT 850
HLFHSGSEET SEEAEQAVDK FGDSYAGDLA DINELKAIME GMESDDYVSD 900
SDWDSDSGRG RGGGDGFDMN HFLDHLEEQG TSLDDLRSFM FRRCRVFFAL 950
PSAGNGDGAA ESKALRLKNY IRFGNGKVVD ELETATHVVV VTAPLGESSK 1000
KEEREIAAEL RYKISLREMG SPMPRIVKGE WVEDSWKEGT VVDEEEYVAG 1050
Length:1,050
Mass (Da):119,433
Last modified:March 19, 2014 - v2
Checksum:iCDA8E04CF37AFA99
GO

Sequence cautioni

The sequence BAF34364.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB261106 Genomic DNA. Translation: BAF34364.1. Sequence problems.
CM002238 Genomic DNA. Translation: EAA33632.2.
RefSeqiXP_962868.1. XM_957775.1.
UniGeneiNcr.24770.

Genome annotation databases

EnsemblFungiiEFNCRT00000006055; EFNCRP00000006048; EFNCRG00000006045.
GeneIDi3879007.
KEGGincr:NCU06264.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB261106 Genomic DNA. Translation: BAF34364.1 . Sequence problems.
CM002238 Genomic DNA. Translation: EAA33632.2 .
RefSeqi XP_962868.1. XM_957775.1.
UniGenei Ncr.24770.

3D structure databases

ModBasei Search...

Protein-protein interaction databases

STRINGi 5141.NCU06264.1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii EFNCRT00000006055 ; EFNCRP00000006048 ; EFNCRG00000006045 .
GeneIDi 3879007.
KEGGi ncr:NCU06264.

Phylogenomic databases

eggNOGi COG1793.
HOGENOMi HOG000176213.
KOi K10777.
OMAi HMCPSTK.
OrthoDBi EOG7QC84G.

Family and domain databases

Gene3Di 1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
3.40.50.10190. 2 hits.
InterProi IPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view ]
Pfami PF00533. BRCT. 1 hit.
PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view ]
SMARTi SM00292. BRCT. 2 hits.
[Graphical view ]
SUPFAMi SSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 2 hits.
TIGRFAMsi TIGR00574. dnl1. 1 hit.
PROSITEi PS50172. BRCT. 2 hits.
PS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nonhomologous chromosomal integration of foreign DNA is completely dependent on MUS-53 (human Lig4 homolog) in Neurospora."
    Ishibashi K., Suzuki K., Ando Y., Takakura C., Inoue H.
    Proc. Natl. Acad. Sci. U.S.A. 103:14871-14876(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Entry informationi

Entry nameiDNLI4_NEUCR
AccessioniPrimary (citable) accession number: Q7SB49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: March 19, 2014
Last modified: July 9, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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