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Q7SB49 (DNLI4_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase 4

EC=6.5.1.1
Alternative name(s):
DNA ligase IV
Mutagen-sensitive protein 53
Polydeoxyribonucleotide synthase [ATP] 4
Gene names
Name:mus-53
Synonyms:lig4
ORF Names:NCU06264
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]
Taxonomic identifier367110 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora

Protein attributes

Sequence length1050 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining. Ref.1

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactor

Magnesium By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Contains 2 BRCT domains.

Sequence caution

The sequence BAF34364.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10501050DNA ligase 4
PRO_0000278384

Regions

Domain742 – 84099BRCT 1
Domain936 – 1049114BRCT 2

Sites

Active site3311N6-AMP-lysine intermediate By similarity
Metal binding3981Magnesium 1 Potential
Metal binding4981Magnesium 2 Potential
Binding site3291ATP By similarity
Binding site3361ATP By similarity
Binding site3581ATP By similarity
Binding site5031ATP By similarity
Binding site5141ATP By similarity
Binding site5201ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7SB49 [UniParc].

Last modified March 19, 2014. Version 2.
Checksum: CDA8E04CF37AFA99

FASTA1,050119,433
        10         20         30         40         50         60 
MNTNRRSRSP DEEALEEDQH QYGAGTLSLE ELDEHFPNRP RNHSKTFPFS DLFRTLFNPL 

        70         80         90        100        110        120 
IDCKPSTSGG TVRGPKPGRG GHFSKVSYHE QRRHIIERFM SRWRSEVGND FYPAMRLILP 

       130        140        150        160        170        180 
DKDRDRGVYG LKENTIGKLL VKVMKIDRNS EDGYNLMHWK LPGGQSGVSR SVGDFAGRCL 

       190        200        210        220        230        240 
EVVSKRAMRA QPGDLTIADV NVLLDRLAAA SGEAEQLPIF EEFYRQMNAE EMMWLVRIIL 

       250        260        270        280        290        300 
KDMRVGATER TFLNLWHPDA EALFSVSSSL RRVCWELFDP EFRLEQQETG IKLMQCFQPQ 

       310        320        330        340        350        360 
LAQFQMTTTW EKLVKNLGVT EENPEFWIEE KLDGERMQMH MIEDDTVPGG FRFAFWSRKA 

       370        380        390        400        410        420 
KDYTYLYGES LGDEQSALTR HLHKAFDDGV RNLILDGEMI TWDIDIDKMV PFGTLKTAAL 

       430        440        450        460        470        480 
EQQKNPSKAG PRPLYRVFDI LLLNDKPLTE YTLNDRRRAL ERAVVGVHRR LEILPFERAT 

       490        500        510        520        530        540 
SPDAIEPLLR RVVAEASEGL VLKNPRSRYS LNSRNNDWIK VKPEYMSDFG ESLDCVVVGG 

       550        560        570        580        590        600 
YFGSGRRGGT LSSFLCGVRV SQNFIKSGNA SAEKCLSFVK VGGGFKAEDY AEIRHHTEGK 

       610        620        630        640        650        660 
WQDWDPSSPP TEYIELGGGE KLQYEKPDVW IRPSDSVVIS VKAASITQSD QFAMGWTLRF 

       670        680        690        700        710        720 
PRFRKLRLDR AWDSALDMDE FEVLRSKIKD QEQERKKMEM ENRKRKPATK RARKDLVIAG 

       730        740        750        760        770        780 
MSDPSSSSAA TPVIAPKETR EASKRLFEGL DFCVLSDSLK PNKMTKPALE KLIKDHGGRI 

       790        800        810        820        830        840 
HQQVMDHSGQ GKIIIPIADK NVIKVASLRK ANPEMDIIRP KWIFDCLVQP MPFTKQKENK 

       850        860        870        880        890        900 
KGYLLPFEPT HLFHSGSEET SEEAEQAVDK FGDSYAGDLA DINELKAIME GMESDDYVSD 

       910        920        930        940        950        960 
SDWDSDSGRG RGGGDGFDMN HFLDHLEEQG TSLDDLRSFM FRRCRVFFAL PSAGNGDGAA 

       970        980        990       1000       1010       1020 
ESKALRLKNY IRFGNGKVVD ELETATHVVV VTAPLGESSK KEEREIAAEL RYKISLREMG 

      1030       1040       1050 
SPMPRIVKGE WVEDSWKEGT VVDEEEYVAG 

« Hide

References

« Hide 'large scale' references
[1]"Nonhomologous chromosomal integration of foreign DNA is completely dependent on MUS-53 (human Lig4 homolog) in Neurospora."
Ishibashi K., Suzuki K., Ando Y., Takakura C., Inoue H.
Proc. Natl. Acad. Sci. U.S.A. 103:14871-14876(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]"The genome sequence of the filamentous fungus Neurospora crassa."
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. expand/collapse author list , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB261106 Genomic DNA. Translation: BAF34364.1. Sequence problems.
CM002238 Genomic DNA. Translation: EAA33632.2.
RefSeqXP_962868.1. XM_957775.1.
UniGeneNcr.24770.

3D structure databases

ProteinModelPortalQ7SB49.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5141.NCU06264.1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFNCRT00000006055; EFNCRP00000006048; EFNCRG00000006045.
GeneID3879007.
KEGGncr:NCU06264.

Phylogenomic databases

eggNOGCOG1793.
HOGENOMHOG000176213.
KOK10777.
OMAHMCPSTK.
OrthoDBEOG7QC84G.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
3.40.50.10190. 2 hits.
InterProIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF00533. BRCT. 1 hit.
PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SMARTSM00292. BRCT. 2 hits.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 2 hits.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS50172. BRCT. 2 hits.
PS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI4_NEUCR
AccessionPrimary (citable) accession number: Q7SB49
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: March 19, 2014
Last modified: April 16, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families