DNA ligase 4 - Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

Preferred order of sections

Names and origin

Protein names

Recommended name:
DNA ligase 4
EC=6.5.1.1

Alternative name(s):
DNA ligase IV
DNA repair and recombination protein mus-53
Polydeoxyribonucleotide synthase [ATP] 4

Gene names

Name:	lig-4
Synonyms:	mus-53
ORF Names:	NCU06264

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora ›

Protein attributes

Sequence length	1046 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining. Ref.1
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Cofactor	Magnesium By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family. Contains 2 BRCT domains.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair DNA replication
Cellular component	Nucleus
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA ligation involved in DNA repair Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW double-strand break repair via nonhomologous end joining Inferred from Biological aspect of Ancestor. Source: RefGenome lagging strand elongation Inferred from Biological aspect of Ancestor. Source: RefGenome nucleotide-excision repair Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	DNA ligase IV complex Inferred from Biological aspect of Ancestor. Source: RefGenome chromosome Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from Biological aspect of Ancestor. Source: RefGenome metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1046

1046

DNA ligase 4

PRO_0000278384

Regions

Domain

738 – 836

99

BRCT 1

Domain

932 – 1045

114

BRCT 2

Sites

Active site

327

1

N6-AMP-lysine intermediate By similarity

Metal binding

394

1

Magnesium 1 Potential

Metal binding

494

1

Magnesium 2 Potential

Binding site

325

1

ATP By similarity

Binding site

332

1

ATP By similarity

Binding site

354

1

ATP By similarity

Binding site

499

1

ATP By similarity

Binding site

510

1

ATP By similarity

Binding site

516

1

ATP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q7SB49 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: 072B0B5B806A3146
Show »

FASTA

1,046

118,910

        10         20         30         40         50         60 
MNTNRRSRSP DEEALEEDQH QYGAGTLSLE ELDEQPRNHS KTFPFSDLFR TLFNPLIDCK 

        70         80         90        100        110        120 
PSTSGGTVRG PKPGRGGHFS KVSYHEQRRH IIERFMSRWR SEVGNDFYPA MRLILPDKDR 

       130        140        150        160        170        180 
DRGVYGLKEN TIGKLLVKVM KIDRNSEDGY NLMHWKLPGG QSGVSRSVGD FAGRCLEVVS 

       190        200        210        220        230        240 
KRAMRAQPGD LTIADVNVLL DRLAAASGEA EQLPIFEEFY RQMNAEEMMW LVRIILKDMR 

       250        260        270        280        290        300 
VGATERTFLN LWHPDAEALF SVSSSLRRVC WELFDPEFRL EQQETGIKLM QCFQPQLAQF 

       310        320        330        340        350        360 
QMTTTWEKLV KNLGVTEENP EFWIEEKLDG ERMQMHMIED DTVPGGFRFA FWSRKAKDYT 

       370        380        390        400        410        420 
YLYGESLGDE QSALTRHLHK AFDDGVRNLI LDGEMITWDI DIDKMVPFGT LKTAALEQQK 

       430        440        450        460        470        480 
NPSKAGPRPL YRVFDILLLN DKPLTEYTLN DRRRALERAV VGVHRRLEIL PFERATSPDA 

       490        500        510        520        530        540 
IEPLLRRVVA EASEGLVLKN PRSRYSLNSR NNDWIKVKPE YMSDFGESLD CVVVGGYFGS 

       550        560        570        580        590        600 
GRRGGTLSSF LCGVRVSQNF IKSGNASAEK CLSFVKVGGG FKAEDYAEIR HHTEGKWQDW 

       610        620        630        640        650        660 
DPSSPPTEYI ELGGGEKLQY EKPDVWIRPS DSVVISVKAA SITQSDQFAM GWTLRFPRFR 

       670        680        690        700        710        720 
KLRLDRAWDS ALDMDEFEVL RSKIKDQEQE RKKMEMENRK RKPATKRARK DLVIAGMSDP 

       730        740        750        760        770        780 
SSSSAATPVI APKETREASK RLFEGLDFCV LSDSLKPNKM TKPALEKLIK DHGGRIHQQV 

       790        800        810        820        830        840 
MDHSGQGKII IPIADKNVIK VASLRKANPE MDIIRPKWIF DCLVQPMPFT KQKENKKGYL 

       850        860        870        880        890        900 
LPFEPTHLFH SGSEETSEEA EQAVDKFGDS YAGDLADINE LKAIMEGMES DDYVSDSDWD 

       910        920        930        940        950        960 
SDSGRGRGGG DGFDMNHFLD HLEEQGTSLD DLRSFMFRRC RVFFALPSAG NGDGAAESKA 

       970        980        990       1000       1010       1020 
LRLKNYIRFG NGKVVDELET ATHVVVVTAP LGESSKKEER EIAAELRYKI SLREMGSPMP 

      1030       1040 
RIVKGEWVED SWKEGTVVDE EEYVAG

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nonhomologous chromosomal integration of foreign DNA is completely dependent on MUS-53 (human Lig4 homolog) in Neurospora." Ishibashi K., Suzuki K., Ando Y., Takakura C., Inoue H. Proc. Natl. Acad. Sci. U.S.A. 103:14871-14876(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. Birren B.W. Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB261106 Genomic DNA. Translation: BAF34364.1. AABX02000025 Genomic DNA. Translation: EAA33632.1.
RefSeq	XP_962868.1. XM_957775.1.
UniGene	Ncr.24770.
3D structure databases
ProteinModelPortal	Q7SB49.
ModBase	Search...
Protein-protein interaction databases
STRING	5141.NCU06264.1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000006055; EFNCRP00000006048; EFNCRG00000006045.
GeneID	3879007.
KEGG	ncr:NCU06264.
Phylogenomic databases
eggNOG	COG1793.
HOGENOM	HOG000176213.
KO	K10777.
OMA	HMCPSTK.
OrthoDB	EOG41K2MD.
Family and domain databases
Gene3D	1.10.3260.10. 1 hit. 2.40.50.140. 1 hit. 3.40.50.10190. 2 hits.
InterPro	IPR001357. BRCT_dom. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. [Graphical view]
Pfam	PF00533. BRCT. 1 hit. PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 2 hits. [Graphical view]
SUPFAM	SSF52113. BRCT. 2 hits. SSF50249. Nucleic_acid_OB. 1 hit. SSF117018. SSF117018. 1 hit.
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS50172. BRCT. 2 hits. PS00697. DNA_LIGASE_A1. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI4_NEUCR

Accession

Primary (citable) accession number: Q7SB49

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	December 15, 2003
Last modified:	May 29, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents