ID   SEN34_NEUCR             Reviewed;         315 AA.
AC   Q7SAK9;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   24-JAN-2024, entry version 116.
DE   RecName: Full=Probable tRNA-splicing endonuclease subunit tsp-4;
DE            EC=4.6.1.16;
DE   AltName: Full=tRNA-intron endonuclease sen34;
DE   AltName: Full=tRNA-splicing protein 2;
GN   Name=tsp-4; Synonyms=sen34; ORFNames=B2E7.010, NCU08032;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12655011; DOI=10.1093/nar/gkg293;
RA   Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA   Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT   "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT   genome sequence.";
RL   Nucleic Acids Res. 31:1944-1954(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Constitutes one of the two catalytic subunit of the tRNA-
CC       splicing endonuclease complex, a complex responsible for identification
CC       and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at
CC       the 5'- and 3'-splice sites to release the intron. The products are an
CC       intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and
CC       5'-OH termini. There are no conserved sequences at the splice sites,
CC       but the intron is invariably located at the same site in the gene,
CC       placing the splice sites an invariant distance from the constant
CC       structural features of the tRNA body. It probably carries the active
CC       site for 3'-splice site cleavage (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-
CC         half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with
CC         a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.; EC=4.6.1.16;
CC   -!- SUBUNIT: tRNA splicing endonuclease is a heterotetramer composed of
CC       tsp-2/sen2, tsp-1/sen15, tsp-4/sen34 and tsp-5/sen54. Interacts
CC       directly with tsp-1/sen15 (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the tRNA-intron endonuclease family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BX897675; CAE85532.1; -; Genomic_DNA.
DR   EMBL; CM002239; EAA33466.1; -; Genomic_DNA.
DR   RefSeq; XP_962702.1; XM_957609.1.
DR   AlphaFoldDB; Q7SAK9; -.
DR   SMR; Q7SAK9; -.
DR   STRING; 367110.Q7SAK9; -.
DR   PaxDb; 5141-EFNCRP00000008294; -.
DR   EnsemblFungi; EAA33466; EAA33466; NCU08032.
DR   GeneID; 3878809; -.
DR   KEGG; ncr:NCU08032; -.
DR   VEuPathDB; FungiDB:NCU08032; -.
DR   HOGENOM; CLU_049366_0_0_1; -.
DR   InParanoid; Q7SAK9; -.
DR   OMA; FIAYPGD; -.
DR   OrthoDB; 35871at2759; -.
DR   Proteomes; UP000001805; Chromosome 4, Linkage Group IV.
DR   GO; GO:0000214; C:tRNA-intron endonuclease complex; IEA:InterPro.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0000213; F:tRNA-intron endonuclease activity; IBA:GO_Central.
DR   GO; GO:0000379; P:tRNA-type intron splice site recognition and cleavage; IBA:GO_Central.
DR   CDD; cd22363; tRNA-intron_lyase_C; 1.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   InterPro; IPR036167; tRNA_intron_Endo_cat-like_sf.
DR   InterPro; IPR006677; tRNA_intron_Endonuc_cat-like.
DR   InterPro; IPR016690; tRNA_splic_SEN34.
DR   PANTHER; PTHR13070:SF0; TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN34; 1.
DR   PANTHER; PTHR13070; TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN34-RELATED; 1.
DR   Pfam; PF01974; tRNA_int_endo; 1.
DR   PIRSF; PIRSF017250; tRNA_splic_SEN34; 1.
DR   SUPFAM; SSF53032; tRNA-intron endonuclease catalytic domain-like; 1.
PE   3: Inferred from homology;
KW   Lyase; Reference proteome; tRNA processing.
FT   CHAIN           1..315
FT                   /note="Probable tRNA-splicing endonuclease subunit tsp-4"
FT                   /id="PRO_0000109466"
FT   REGION          117..138
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          169..188
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        247
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        255
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        286
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   315 AA;  34841 MW;  11F689807DC9F27F CRC64;
     MSSTEDDRQV RISKIAGRYL VFDIDDVAYI RRQHGICAVF TGTMPQNPTQ NVFLGLPLEL
     YAEDAKMLLD RKVAYIADDP LEHLTQLKSM DDETRKSYLQ SIKTQRRTAQ LVFNEAKAQS
     MAKHKDKRKP KQPQPSVVVP TELPLSASSA DGVAAAVDQE EEGGAEFLLE SSSSKPLKTD
     ASTPPQKPLV KEKLPAITPT TSNAMISNGT SNPDVENHTA LPLYSYLNER GYFITPGLRF
     GGDFSVYPGD PFRYHAHYMA NSYGWDEKIP MLDLVTSGRL GTAVKKSFLM GGQKPATEDS
     EAGELRAFCI EWAGM
//