Q7SAK9 (SEN34_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable tRNA-splicing endonuclease subunit sen-34
EC=3.1.27.9

Alternative name(s):
tRNA-intron endonuclease sen-34

Gene names

Name:	sen-34
ORF Names:	B2E7.010, NCU08032

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora › Neurospora crassa

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage By similarity.
Catalytic activity	Endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxy and 2',3'-cyclic phosphate termini, and specifically removing the intron.
Subunit structure	tRNA splicing endonuclease is a heterotetramer composed of sen-2, sen-15, sen-34 and sen-54. Interacts directly with sen-15 By similarity.
Sequence similarities	Belongs to the tRNA-intron endonuclease family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA-type intron splice site recognition and cleavage Inferred from electronic annotation. Source: InterPro
Cellular_component	tRNA-intron endonuclease complex Inferred from electronic annotation. Source: InterPro
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro tRNA-intron endonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Probable tRNA-splicing endonuclease subunit sen-34		PRO_0000109466
Sites
	Active site	247	1	By similarity
	Active site	255	1	By similarity
	Active site	286	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7SAK9 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 11F689807DC9F27F	FASTA	315	34,841
	10 20 30 40 50 60 MSSTEDDRQV RISKIAGRYL VFDIDDVAYI RRQHGICAVF TGTMPQNPTQ NVFLGLPLEL 70 80 90 100 110 120 YAEDAKMLLD RKVAYIADDP LEHLTQLKSM DDETRKSYLQ SIKTQRRTAQ LVFNEAKAQS 130 140 150 160 170 180 MAKHKDKRKP KQPQPSVVVP TELPLSASSA DGVAAAVDQE EEGGAEFLLE SSSSKPLKTD 190 200 210 220 230 240 ASTPPQKPLV KEKLPAITPT TSNAMISNGT SNPDVENHTA LPLYSYLNER GYFITPGLRF 250 260 270 280 290 300 GGDFSVYPGD PFRYHAHYMA NSYGWDEKIP MLDLVTSGRL GTAVKKSFLM GGQKPATEDS 310 EAGELRAFCI EWAGM

References

[1]

"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence." Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U. Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

[2]

"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX897675 Genomic DNA. Translation: CAE85532.1. AABX02000051 Genomic DNA. Translation: EAA33466.1.
RefSeq	XP_962702.1. XM_957609.1.
3D structure databases
ProteinModelPortal	Q7SAK9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000008308; EFNCRP00000008294; EFNCRG00000008294.
GeneID	3878809.
KEGG	ncr:NCU08032.
Phylogenomic databases
eggNOG	COG1676.
HOGENOM	HOG000175584.
KO	K15323.
OMA	VEESTEY.
OrthoDB	EOG4WDHN7.
Family and domain databases
Gene3D	3.40.1350.10. 1 hit.
InterPro	IPR011856. tRNA_endonuc-like_dom. IPR006677. tRNA_intron_Endonuc_cat-like. IPR016690. tRNA_splic_SEN34. [Graphical view]
Pfam	PF01974. tRNA_int_endo. 1 hit. [Graphical view]
PIRSF	PIRSF017250. tRNA_splic_SEN34. 1 hit.
SUPFAM	SSF53032. tRNA_int_endo_C. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SEN34_NEUCR

Accession

Primary (citable) accession number: Q7SAK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	December 15, 2003
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program