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Protein

Probable tRNA-splicing endonuclease subunit tsp-4

Gene

tsp-4

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei247By similarity1
Active sitei255By similarity1
Active sitei286By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processtRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA-splicing endonuclease subunit tsp-4 (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease sen34
tRNA-splicing protein 2
Gene namesi
Name:tsp-4
Synonyms:sen34
ORF Names:B2E7.010, NCU08032
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 4, Linkage Group IV

Organism-specific databases

EuPathDBiFungiDB:NCU08032

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094661 – 315Probable tRNA-splicing endonuclease subunit tsp-4Add BLAST315

Interactioni

Subunit structurei

tRNA splicing endonuclease is a heterotetramer composed of tsp-2/sen2, tsp-1/sen15, tsp-4/sen34 and tsp-5/sen54. Interacts directly with tsp-1/sen15 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ7SAK9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family.Curated

Phylogenomic databases

HOGENOMiHOG000175584
InParanoidiQ7SAK9
KOiK15323
OMAiRTFSIEW
OrthoDBiEOG092C3X38

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR016690 tRNA_splic_SEN34
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 1 hit
PIRSFiPIRSF017250 tRNA_splic_SEN34, 1 hit
SUPFAMiSSF53032 SSF53032, 1 hit

Sequencei

Sequence statusi: Complete.

Q7SAK9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTEDDRQV RISKIAGRYL VFDIDDVAYI RRQHGICAVF TGTMPQNPTQ
60 70 80 90 100
NVFLGLPLEL YAEDAKMLLD RKVAYIADDP LEHLTQLKSM DDETRKSYLQ
110 120 130 140 150
SIKTQRRTAQ LVFNEAKAQS MAKHKDKRKP KQPQPSVVVP TELPLSASSA
160 170 180 190 200
DGVAAAVDQE EEGGAEFLLE SSSSKPLKTD ASTPPQKPLV KEKLPAITPT
210 220 230 240 250
TSNAMISNGT SNPDVENHTA LPLYSYLNER GYFITPGLRF GGDFSVYPGD
260 270 280 290 300
PFRYHAHYMA NSYGWDEKIP MLDLVTSGRL GTAVKKSFLM GGQKPATEDS
310
EAGELRAFCI EWAGM
Length:315
Mass (Da):34,841
Last modified:December 15, 2003 - v1
Checksum:i11F689807DC9F27F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX897675 Genomic DNA Translation: CAE85532.1
CM002239 Genomic DNA Translation: EAA33466.1
RefSeqiXP_962702.1, XM_957609.1

Genome annotation databases

EnsemblFungiiEAA33466; EAA33466; NCU08032
GeneIDi3878809
KEGGincr:NCU08032

Similar proteinsi

Entry informationi

Entry nameiSEN34_NEUCR
AccessioniPrimary (citable) accession number: Q7SAK9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: December 15, 2003
Last modified: March 28, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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