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Q7SAK9

- SEN34_NEUCR

UniProt

Q7SAK9 - SEN34_NEUCR

Protein

Probable tRNA-splicing endonuclease subunit tsp-4

Gene

tsp-4

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (15 Dec 2003)
      Previous versions | rss
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    Functioni

    Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage By similarity.By similarity

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei247 – 2471By similarity
    Active sitei255 – 2551By similarity
    Active sitei286 – 2861By similarity

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: InterPro

    GO - Biological processi

    1. tRNA-type intron splice site recognition and cleavage Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    tRNA processing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable tRNA-splicing endonuclease subunit tsp-4 (EC:4.6.1.16)
    Alternative name(s):
    tRNA-intron endonuclease sen34
    tRNA-splicing protein 2
    Gene namesi
    Name:tsp-4
    Synonyms:sen34
    ORF Names:B2E7.010, NCU08032
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Chromosome 4, Linkage Group IV

    Subcellular locationi

    GO - Cellular componenti

    1. tRNA-intron endonuclease complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 315315Probable tRNA-splicing endonuclease subunit tsp-4PRO_0000109466Add
    BLAST

    Interactioni

    Subunit structurei

    tRNA splicing endonuclease is a heterotetramer composed of tsp-2/sen2, tsp-1/sen15, tsp-4/sen34 and tsp-5/sen54. Interacts directly with tsp-1/sen15 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi5141.NCU08032.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7SAK9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family.Curated

    Phylogenomic databases

    eggNOGiCOG1676.
    HOGENOMiHOG000175584.
    KOiK15323.
    OMAiRTFSIEW.
    OrthoDBiEOG7N905X.

    Family and domain databases

    Gene3Di3.40.1350.10. 1 hit.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR016690. tRNA_splic_SEN34.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017250. tRNA_splic_SEN34. 1 hit.
    SUPFAMiSSF53032. SSF53032. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q7SAK9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSTEDDRQV RISKIAGRYL VFDIDDVAYI RRQHGICAVF TGTMPQNPTQ    50
    NVFLGLPLEL YAEDAKMLLD RKVAYIADDP LEHLTQLKSM DDETRKSYLQ 100
    SIKTQRRTAQ LVFNEAKAQS MAKHKDKRKP KQPQPSVVVP TELPLSASSA 150
    DGVAAAVDQE EEGGAEFLLE SSSSKPLKTD ASTPPQKPLV KEKLPAITPT 200
    TSNAMISNGT SNPDVENHTA LPLYSYLNER GYFITPGLRF GGDFSVYPGD 250
    PFRYHAHYMA NSYGWDEKIP MLDLVTSGRL GTAVKKSFLM GGQKPATEDS 300
    EAGELRAFCI EWAGM 315
    Length:315
    Mass (Da):34,841
    Last modified:December 15, 2003 - v1
    Checksum:i11F689807DC9F27F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX897675 Genomic DNA. Translation: CAE85532.1.
    CM002239 Genomic DNA. Translation: EAA33466.1.
    RefSeqiXP_962702.1. XM_957609.1.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000008308; EFNCRP00000008294; EFNCRG00000008294.
    GeneIDi3878809.
    KEGGincr:NCU08032.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX897675 Genomic DNA. Translation: CAE85532.1 .
    CM002239 Genomic DNA. Translation: EAA33466.1 .
    RefSeqi XP_962702.1. XM_957609.1.

    3D structure databases

    ProteinModelPortali Q7SAK9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5141.NCU08032.1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFNCRT00000008308 ; EFNCRP00000008294 ; EFNCRG00000008294 .
    GeneIDi 3878809.
    KEGGi ncr:NCU08032.

    Phylogenomic databases

    eggNOGi COG1676.
    HOGENOMi HOG000175584.
    KOi K15323.
    OMAi RTFSIEW.
    OrthoDBi EOG7N905X.

    Family and domain databases

    Gene3Di 3.40.1350.10. 1 hit.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR016690. tRNA_splic_SEN34.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017250. tRNA_splic_SEN34. 1 hit.
    SUPFAMi SSF53032. SSF53032. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
      Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
      Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    2. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

    Entry informationi

    Entry nameiSEN34_NEUCR
    AccessioniPrimary (citable) accession number: Q7SAK9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: December 15, 2003
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3