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Protein

Probable tRNA-splicing endonuclease subunit tsp-4

Gene

tsp-4

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei247 – 2471By similarity
Active sitei255 – 2551By similarity
Active sitei286 – 2861By similarity

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: InterPro

GO - Biological processi

  1. tRNA-type intron splice site recognition and cleavage Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA-splicing endonuclease subunit tsp-4 (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease sen34
tRNA-splicing protein 2
Gene namesi
Name:tsp-4
Synonyms:sen34
ORF Names:B2E7.010, NCU08032
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805 Componenti: Chromosome 4, Linkage Group IV

Subcellular locationi

GO - Cellular componenti

  1. tRNA-intron endonuclease complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Probable tRNA-splicing endonuclease subunit tsp-4PRO_0000109466Add
BLAST

Interactioni

Subunit structurei

tRNA splicing endonuclease is a heterotetramer composed of tsp-2/sen2, tsp-1/sen15, tsp-4/sen34 and tsp-5/sen54. Interacts directly with tsp-1/sen15 (By similarity).By similarity

Protein-protein interaction databases

STRINGi5141.NCU08032.1.

Structurei

3D structure databases

ProteinModelPortaliQ7SAK9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family.Curated

Phylogenomic databases

eggNOGiCOG1676.
HOGENOMiHOG000175584.
InParanoidiQ7SAK9.
KOiK15323.
OMAiRTFSIEW.
OrthoDBiEOG7N905X.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR016690. tRNA_splic_SEN34.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 1 hit.
[Graphical view]
PIRSFiPIRSF017250. tRNA_splic_SEN34. 1 hit.
SUPFAMiSSF53032. SSF53032. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7SAK9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTEDDRQV RISKIAGRYL VFDIDDVAYI RRQHGICAVF TGTMPQNPTQ
60 70 80 90 100
NVFLGLPLEL YAEDAKMLLD RKVAYIADDP LEHLTQLKSM DDETRKSYLQ
110 120 130 140 150
SIKTQRRTAQ LVFNEAKAQS MAKHKDKRKP KQPQPSVVVP TELPLSASSA
160 170 180 190 200
DGVAAAVDQE EEGGAEFLLE SSSSKPLKTD ASTPPQKPLV KEKLPAITPT
210 220 230 240 250
TSNAMISNGT SNPDVENHTA LPLYSYLNER GYFITPGLRF GGDFSVYPGD
260 270 280 290 300
PFRYHAHYMA NSYGWDEKIP MLDLVTSGRL GTAVKKSFLM GGQKPATEDS
310
EAGELRAFCI EWAGM
Length:315
Mass (Da):34,841
Last modified:December 14, 2003 - v1
Checksum:i11F689807DC9F27F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX897675 Genomic DNA. Translation: CAE85532.1.
CM002239 Genomic DNA. Translation: EAA33466.1.
RefSeqiXP_962702.1. XM_957609.1.

Genome annotation databases

EnsemblFungiiEFNCRT00000008308; EFNCRP00000008294; EFNCRG00000008294.
GeneIDi3878809.
KEGGincr:NCU08032.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX897675 Genomic DNA. Translation: CAE85532.1.
CM002239 Genomic DNA. Translation: EAA33466.1.
RefSeqiXP_962702.1. XM_957609.1.

3D structure databases

ProteinModelPortaliQ7SAK9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5141.NCU08032.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEFNCRT00000008308; EFNCRP00000008294; EFNCRG00000008294.
GeneIDi3878809.
KEGGincr:NCU08032.

Phylogenomic databases

eggNOGiCOG1676.
HOGENOMiHOG000175584.
InParanoidiQ7SAK9.
KOiK15323.
OMAiRTFSIEW.
OrthoDBiEOG7N905X.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR016690. tRNA_splic_SEN34.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 1 hit.
[Graphical view]
PIRSFiPIRSF017250. tRNA_splic_SEN34. 1 hit.
SUPFAMiSSF53032. SSF53032. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
    Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
    Nucleic Acids Res. 31:1944-1954(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  2. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Entry informationi

Entry nameiSEN34_NEUCR
AccessioniPrimary (citable) accession number: Q7SAK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 18, 2004
Last sequence update: December 14, 2003
Last modified: January 6, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.