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Q7SAK9 (SEN34_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable tRNA-splicing endonuclease subunit tsp-4

EC=4.6.1.16
Alternative name(s):
tRNA-intron endonuclease sen34
tRNA-splicing protein 2
Gene names
Name:tsp-4
Synonyms:sen34
ORF Names:B2E7.010, NCU08032
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]
Taxonomic identifier367110 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage By similarity.

Catalytic activity

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Subunit structure

tRNA splicing endonuclease is a heterotetramer composed of tsp-2/sen2, tsp-1/sen15, tsp-4/sen34 and tsp-5/sen54. Interacts directly with tsp-1/sen15 By similarity.

Sequence similarities

Belongs to the tRNA-intron endonuclease family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 315315Probable tRNA-splicing endonuclease subunit tsp-4
PRO_0000109466

Sites

Active site2471 By similarity
Active site2551 By similarity
Active site2861 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7SAK9 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: 11F689807DC9F27F

FASTA31534,841
        10         20         30         40         50         60 
MSSTEDDRQV RISKIAGRYL VFDIDDVAYI RRQHGICAVF TGTMPQNPTQ NVFLGLPLEL 

        70         80         90        100        110        120 
YAEDAKMLLD RKVAYIADDP LEHLTQLKSM DDETRKSYLQ SIKTQRRTAQ LVFNEAKAQS 

       130        140        150        160        170        180 
MAKHKDKRKP KQPQPSVVVP TELPLSASSA DGVAAAVDQE EEGGAEFLLE SSSSKPLKTD 

       190        200        210        220        230        240 
ASTPPQKPLV KEKLPAITPT TSNAMISNGT SNPDVENHTA LPLYSYLNER GYFITPGLRF 

       250        260        270        280        290        300 
GGDFSVYPGD PFRYHAHYMA NSYGWDEKIP MLDLVTSGRL GTAVKKSFLM GGQKPATEDS 

       310 
EAGELRAFCI EWAGM 

« Hide

References

[1]"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]"The genome sequence of the filamentous fungus Neurospora crassa."
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. expand/collapse author list , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX897675 Genomic DNA. Translation: CAE85532.1.
CM002239 Genomic DNA. Translation: EAA33466.1.
RefSeqXP_962702.1. XM_957609.1.

3D structure databases

ProteinModelPortalQ7SAK9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5141.NCU08032.1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFNCRT00000008308; EFNCRP00000008294; EFNCRG00000008294.
GeneID3878809.
KEGGncr:NCU08032.

Phylogenomic databases

eggNOGCOG1676.
HOGENOMHOG000175584.
KOK15323.
OMARTFSIEW.
OrthoDBEOG7N905X.

Family and domain databases

Gene3D3.40.1350.10. 1 hit.
InterProIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR016690. tRNA_splic_SEN34.
[Graphical view]
PfamPF01974. tRNA_int_endo. 1 hit.
[Graphical view]
PIRSFPIRSF017250. tRNA_splic_SEN34. 1 hit.
SUPFAMSSF53032. SSF53032. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSEN34_NEUCR
AccessionPrimary (citable) accession number: Q7SAK9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: December 15, 2003
Last modified: June 11, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families