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Protein

Lysophospholipase nte1

Gene

nte1

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium (By similarity).By similarity

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Inhibited by organophosphorus esters.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1245NucleophilePROSITE-ProRule annotation1
Active sitei1363Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi670 – 789cNMP 1Add BLAST120
Nucleotide bindingi835 – 955cNMP 2Add BLAST121

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Lysophospholipase nte1 (EC:3.1.1.5)
Alternative name(s):
Intracellular phospholipase B
Neuropathy target esterase homolog
Gene namesi
Name:nte1
ORF Names:NCU08276
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 4, Linkage Group IV

Organism-specific databases

EuPathDBiFungiDB:NCU08276.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 59CytoplasmicBy similarityAdd BLAST59
Transmembranei60 – 80HelicalSequence analysisAdd BLAST21
Topological domaini81 – 102LumenalBy similarityAdd BLAST22
Transmembranei103 – 123HelicalSequence analysisAdd BLAST21
Topological domaini124 – 1515CytoplasmicBy similarityAdd BLAST1392

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002953261 – 1515Lysophospholipase nte1Add BLAST1515

Structurei

3D structure databases

ProteinModelPortaliQ7S8J1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1212 – 1376PNPLAPROSITE-ProRule annotationAdd BLAST165

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1216 – 1221GXGXXGPROSITE-ProRule annotation6
Motifi1243 – 1247GXSXGPROSITE-ProRule annotation5
Motifi1363 – 1365DGA/GPROSITE-ProRule annotation3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi280 – 285Poly-Asp6

Sequence similaritiesi

Belongs to the NTE family.Curated
Contains 2 cyclic nucleotide-binding domains.PROSITE-ProRule annotation
Contains 1 PNPLA (patatin-like phospholipase) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000048680.
InParanoidiQ7S8J1.
KOiK14676.
OrthoDBiEOG092C0BAH.

Family and domain databases

Gene3Di2.60.120.10. 5 hits.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR001423. LysoPLipase_patatin_CS.
IPR002641. Patatin/PLipase_A2-rel.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF01734. Patatin. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 2 hits.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 5 hits.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 2 hits.
PS51635. PNPLA. 1 hit.
PS01237. UPF0028. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7S8J1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESLSNLGNA MSSVLSETTS TTATAILADP TEALSSVVAL ASDAVSKATS
60 70 80 90 100
DVVPEHTPTS WFTIILWLLH RISSVLYFVI KLTTITTPTF LFNIFSTSLT
110 120 130 140 150
VTMNATTLVL IMLFMMAGVT WVVRYRYLNM YSRLPPEPQR KEPAVDLFPE
160 170 180 190 200
TQEEGVKSGL ASYFDEFLSA IKIFGYLERP VFHELTRSMQ TRKLIAGETL
210 220 230 240 250
NLEEEQGFCL VVDGMVEIFV KSARDGRRSS SGPSFESDDE EGFAPGRQRY
260 270 280 290 300
QLLTEVRNGA PMSSLFSIMS LFTEDIQMHD TDDDDSPDPT PSAPGTAMPG
310 320 330 340 350
YPMNANFQQS NDTLPSIPGT PLSDNHVMQR GMASAESLTM SPLAHDSSRI
360 370 380 390 400
PSLSLVNTRP AVPRRPVPKR LNTTSAHPDI IARATVDTTI AIIPASAFRR
410 420 430 440 450
LIKIYPKATA HIVHVILSRF QRVTLATAYN YLGLSGEVLQ MERNILKHTV
460 470 480 490 500
RQLPNHLRGD ALDRLKEKFK RERERIGEKE VEKGIALHNA HAARRRRSAA
510 520 530 540 550
SLRKEVALQA YSKRRQSIVT ATPAFGTRRE RPSPDMPSPG DLLTNTQQLK
560 570 580 590 600
SGPASQLDLA RDATSPRPQR NLSPFSTPRN AHVSLDTREA LDEDDMFRES
610 620 630 640 650
VLECMFRAIG LTGNGSVNPD STQASPRFVP TDQRRSRGGP GHTAFGFMDA
660 670 680 690 700
FDPFDNDTES VTSCGFSSSS PVPNPQLLAH DMKDEVEIVF FPKGSVLVEQ
710 720 730 740 750
GERNPGLYYV IDGFLDICVP GDDSSHDLLQ SSAKVDHGHD SATGADAFGA
760 770 780 790 800
NSSRFPEPNM NNNFGAEQKK GKQGRRSVAL IKPGGLAGYV GTISSYRSFI
810 820 830 840 850
DVVARTDVCV GFLPLASIDR IVDRYPIVML TMAKRLTELL PRLLLHIDFA
860 870 880 890 900
LEWVQVNSGQ VLFREGDESE AIYLVLNGRL RLVEDQKDGG MNVKAEFGQG
910 920 930 940 950
ESIGELEVLT ESARTGTLHA IRETELVKFP RTLFNSLAQE HPNITIKISK
960 970 980 990 1000
IIAARMRAFI DDPSRMGLKD NVVRSYKSSS TLNLRTVAIL PVTAGVPVVE
1010 1020 1030 1040 1050
FGNRLMNALS QVGPPNGATC LNQAAILNHL GKLAFNRMGK LKLSQYLADL
1060 1070 1080 1090 1100
EEKYGLVVYV ADTNVNTPWT QTCITQADCL LFVGLADSSP EIGEYERFML
1110 1120 1130 1140 1150
GMKSTARKIL VLVHQERYSN PGLTRQWLKN RMWINGGHFH VQMAYTSNTV
1160 1170 1180 1190 1200
PIHPPTKRFG PSLKQRVQIL QAEIQKYTSR KVRHVPFYSP DAPFKGDFHR
1210 1220 1230 1240 1250
LARRLCGKSV GLVLGGGGAR GIAHVGIIRA MEEAGIPIDM VGGTSIGAFI
1260 1270 1280 1290 1300
GGLYARHADV VPIFGLAKKF AGRMASMWRF AFDLTYPSAS YTTGHEFNRG
1310 1320 1330 1340 1350
IFKTFGKTQI EDFWLEYYCN TTNISKSRVE FHTSGYAWRY IRASMSLAGL
1360 1370 1380 1390 1400
LPPLCDEGSM LLDGGYIDNL TVSRMKSLGS DVIFAVDVGS LDDNTPQAFG
1410 1420 1430 1440 1450
DSLSGVWAFF NRWNPFSSVP NPPTLAEIQA RLAYVSSVGE LERAKTMPGC
1460 1470 1480 1490 1500
IYMRPPIDDY GTLDFGKFDE IYGVGYKYGQ EFLQKLREQG VLPLVEETEA
1510
KKALRRTMAP RRASI
Length:1,515
Mass (Da):167,560
Last modified:July 24, 2007 - v2
Checksum:iBB4572D40BEB6275
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM002239 Genomic DNA. Translation: EAA32668.3.
RefSeqiXP_961904.3. XM_956811.3.

Genome annotation databases

EnsemblFungiiEAA32668; EAA32668; NCU08276.
GeneIDi3878043.
KEGGincr:NCU08276.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM002239 Genomic DNA. Translation: EAA32668.3.
RefSeqiXP_961904.3. XM_956811.3.

3D structure databases

ProteinModelPortaliQ7S8J1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEAA32668; EAA32668; NCU08276.
GeneIDi3878043.
KEGGincr:NCU08276.

Organism-specific databases

EuPathDBiFungiDB:NCU08276.

Phylogenomic databases

HOGENOMiHOG000048680.
InParanoidiQ7S8J1.
KOiK14676.
OrthoDBiEOG092C0BAH.

Family and domain databases

Gene3Di2.60.120.10. 5 hits.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR001423. LysoPLipase_patatin_CS.
IPR002641. Patatin/PLipase_A2-rel.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF01734. Patatin. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 2 hits.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 5 hits.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 2 hits.
PS51635. PNPLA. 1 hit.
PS01237. UPF0028. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNTE1_NEUCR
AccessioniPrimary (citable) accession number: Q7S8J1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: July 24, 2007
Last modified: November 30, 2016
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.