Q7S4Z6 (FDFT_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable squalene synthase
Short name=SQS
Short name=SS
EC=2.5.1.21

Alternative name(s):
FPP:FPP farnesyltransferase
Farnesyl-diphosphate farnesyltransferase

Gene names

Name:	erg-9
ORF Names:	NCU06054

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 farnesyl diphosphate = diphosphate + presqualene diphosphate. Presqualene diphosphate + NAD(P)H = squalene + diphosphate + NAD(P)+.
Cofactor	Magnesium By similarity.
Pathway	Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the phytoene/squalene synthase family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Isoprene biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium NADP
Molecular function	Oxidoreductase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	farnesyl-diphosphate farnesyltransferase activity Inferred from electronic annotation. Source: EC oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW squalene synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 481	481	Probable squalene synthase		PRO_0000067449
Regions
	Transmembrane	294 – 314	21	Helical; Potential
	Transmembrane	416 – 436	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7S4Z6 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 59ED6533600BF4DC	FASTA	481	54,942
	10 20 30 40 50 60 MGAFTKAAYF LLHPNQLRSI VQWKVWHEPV HRRDPSKETE TEKACFRHLE LTSRSFSAVI 70 80 90 100 110 120 QELNPELLMP ICLFYLVLRG LDTIEDDMTI DLAKKEPLLR EFADLMEIDG WTFTENGPNE 130 140 150 160 170 180 KDRELLVHFD DVIAELKKVK KPYYDIIREI TVKMGNGMAD YALNAEHNTN GVNTIEEYEL 190 200 210 220 230 240 YCHYVAGLVG EGLTRLFVES NLANPALLER MELTESMGQF LQKTNIIRDI HEDYVDKRRF 250 260 270 280 290 300 WPKTIWSKYV NTWDDMFKPE NREKALQCSS EMVLNALKHT EDCLFYMAGM RDQSVFNFVA 310 320 330 340 350 360 IPQAMAIATL ELVFRNPAIF ERNVKITKGD ACQLMMESTQ NLRVVCEVFR RYARRIHKKN 370 380 390 400 410 420 DPRDPNYLAI SVQCGKIEQF IESIFPTQDP KKIALAQAQN SNQTAANTTD NGDTTFLVLS 430 440 450 460 470 480 MIGVLFVMGG LMIGAAWLMG ARFDMAYEDI TARVGTLVNG AAAVSSATVS SIPTTVMHQE L

References

[1]

"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AABX02000021 Genomic DNA. Translation: EAA30581.1.
RefSeq	XP_959817.1. XM_954724.2.
3D structure databases
ProteinModelPortal	Q7S4Z6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7S4Z6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000005379; EFNCRP00000005373; EFNCRG00000005372.
GeneID	3875964.
KEGG	ncr:NCU06054.
NMPDR	fig|5141.1.peg.3842.
Phylogenomic databases
eggNOG	fuNOG05680.
GeneTree	EFGT00050000006037.
OMA	CSSEMVL.
OrthoDB	EOG4BP4MQ.
Family and domain databases
InterPro	IPR002060. Squ/phyt_synthse. IPR006449. Squal_synth. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Gene3D	G3DSA:1.10.600.10. Terpenoid_synth. 1 hit.
KO	K00801.
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
TIGRFAMs	TIGR01559. Squal_synth. 1 hit.
PROSITE	PS00014. ER_TARGET. 1 hit. Uncertain. PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit. PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDFT_NEUCR

Accession

Primary (citable) accession number: Q7S4Z6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	December 15, 2003
Last modified:	December 14, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program