Reviewed, UniProtKB/Swiss-Prot Q7S3C9 (KMO_NEUCR)

Names and origin

Protein names

Recommended name:
    Kynurenine 3-monooxygenase
    EC=1.14.13.9
Alternative name(s):
    Kynurenine 3-hydroxylase
    Biosynthesis of nicotinic acid protein 4

Gene names

Name:	bna-4
ORF Names:	NCU06924

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	507 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid By similarity.
Catalytic activity	L-kynurenine + NADPH + O2 = 3-hydroxy-L-kynurenine + NADP+ + H2O.
Cofactor	FAD By similarity.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the aromatic-ring hydroxylase family. KMO subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Mitochondrion
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kynurenine 3-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 507	507	Kynurenine 3-monooxygenase		PRO_0000361930

Sequences

	Sequence		Length	Mass (Da)
	Q7S3C9-1 [UniParc]. Last modified October 23, 2007. Version 2. Checksum: AC321E255A69D694	FASTA	507	57,317
	10 20 30 40 50 60 MEERKQKVVV VGAGPVGSLA ALYAANRGHD VEIYELRGDL RDPSTTPLNF TRSINLALSE 70 80 90 100 110 120 RGLNAMRHAN QPRLIDYVKG VTIPMRGRMI HGKRPDGKLY EEAQDYDIHG RVRPLPSTPL 130 140 150 160 170 180 PFYRAEMPNV TFFFNHKLTG ADFKRNKAWF ENKDESTSNP RDRAREIEVD FDFMIGADGA 190 200 210 220 230 240 HSAVRYHLMK FSRMDYQQEY IDTLWCEFQI APSSSSAKSK FRISPNHLHI WPGKEFMFIA 250 260 270 280 290 300 IPSNDGSFTC TLFAPAAIYE QLEEAGRTGD TSSSIPEFFD MHFPGVTSLI APADLIAQFQ 310 320 330 340 350 360 TNPHLPLISI KCKPYHFSSS VVIVGDAAHA MVPFYGQGMN AGLEDVRILF DILDKHDRMT 370 380 390 400 410 420 NDDSSLEASQ RELALAEYSA VRVADAHAIN DLALQNYIEM RSSVLSPVYR WRKALEEWLS 430 440 450 460 470 480 VYVPSLGWQT KYSRVSFGNE RYSEVVKKSE RQGQVLLRSL VGGVGLPMLA GGLFLWFRYK 490 500 GALGRAAYGV FYNCMGMVCR TIHGRRR

References

[1]

"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AABX02000032 Genomic DNA. Translation: EAA30035.2.
RefSeq	XP_959271.2.
3D structure databases
SMR	Q7S3C9. Positions 167-393.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7S3C9.
Genome annotation databases
GeneID	3875379.
KEGG	ncr:NCU06924.
Phylogenomic databases
eggNOG	fuNOG06151.
OrthoDB	EOG9C2JSV.
PhylomeDB	Q7S3C9.
Family and domain databases
InterPro	IPR002938. mOase_FAD_bd. IPR003042. Rng_hydrolase-like. [Graphical view]
Pfam	PF01494. FAD_binding_3. 1 hit. [Graphical view]
PRINTS	PR00420. RNGMNOXGNASE.
ProtoNet	Search...

Entry information

Entry name

KMO_NEUCR

Accession

Primary (citable) accession number: Q7S3C9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	October 23, 2007
Last modified:	January 19, 2010
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)