Q7S3C8 (NMT_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycylpeptide N-tetradecanoyltransferase
EC=2.3.1.97

Alternative name(s):
Myristoyl-CoA:protein N-myristoyltransferase
Short name=NMT
Peptide N-myristoyltransferase

Gene names

Name:	nmt-1
ORF Names:	NCU06925

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	569 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins By similarity.
Catalytic activity	Tetradecanoyl-CoA + glycylpeptide = CoA + N-tetradecanoylglycylpeptide.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NMT family.
Sequence caution	The sequence EAA30036.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	N-terminal protein myristoylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycylpeptide N-tetradecanoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 569	569	Glycylpeptide N-tetradecanoyltransferase		PRO_0000064244
Regions
	Region	158 – 161	4	Myristoyl CoA-binding By similarity
	Region	288 – 324	37	Myristoyl CoA-binding By similarity
Sites
	Active site	569	1	Proton acceptor; via carboxylate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7S3C8 [UniParc]. Last modified January 10, 2006. Version 2. Checksum: 1DBBD286BD6DAA4A	FASTA	569	64,151
	10 20 30 40 50 60 MPPTEESKPV DPAQEKQAAE ALASLKEAQQ PAADESGSED EGADGTAQEA GSGSTKKKNK 70 80 90 100 110 120 KKSKKKNKDK SASTESSAEV GLTEALAQAD PKSALSGLTP KQIQEFIDLN PALANELLAA 130 140 150 160 170 180 SGSSGTTDVM EAFKKLKIQD IITGLASSGK NRKDMASYKF WATQPVPQFD EKPAIFEEGP 190 200 210 220 230 240 LKIQKVEDIP DEPIPLNLAP FRWVTMDLTD EKQMQEVEKL LYGHFVEDDE AMFRFKYSTS 250 260 270 280 290 300 ILKWSLMSPG WRKEWHVGIR SGDTLCAFIA AVPTEIRVRD KVIQGSEVNF LCIHKKLRGK 310 320 330 340 350 360 RLAPVLIKEI TRRINREGIW QAIYTGGIVL PRPVSTCRYY HRALNWQKLY EVGFSPCPSN 370 380 390 400 410 420 SKPAFQVRKY ALPEQTSTKG LRELQVKDLD AVHSLLERYL KRFDLTPVFN REETEHWLLH 430 440 450 460 470 480 KKDSYAEQVI YSYVVEDASG KITDFFSFYL LESTVIRHPK HNSIRAAYMF YYATETAFTE 490 500 510 520 530 540 PFDKGALTKR LNDLMADALI LAKRHNFDVF NALSLMDNAL FLEKQKFGPG DGQLHYYLFN 550 560 YKANPIHGGV DKKNRLDEDN LSGVGFVMV

References

[1]

"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AABX02000032 Genomic DNA. Translation: EAA30036.1. Sequence problems.
RefSeq	XP_959272.1. XM_954179.1.
UniGene	Ncr.16234.
3D structure databases
ProteinModelPortal	Q7S3C8.
SMR	Q7S3C8. Positions 178-569.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7S3C8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000006733; EFNCRP00000006724; EFNCRG00000006721.
GeneID	3875380.
KEGG	ncr:NCU06925.
Phylogenomic databases
eggNOG	fuNOG07746.
GeneTree	EFGT00050000004932.
OrthoDB	EOG4V9XZZ.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000903. MyristoylCoA_TrFase. IPR022677. MyristoylCoA_TrFase_C. IPR022678. MyristoylCoA_TrFase_CS. IPR022676. MyristoylCoA_TrFase_N. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 2 hits.
PANTHER	PTHR11377. Myristoyl_trans. 1 hit.
Pfam	PF01233. NMT. 1 hit. PF02799. NMT_C. 1 hit. [Graphical view]
PIRSF	PIRSF015892. N-myristl_transf. 1 hit.
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 2 hits.
PROSITE	PS00975. NMT_1. 1 hit. PS00976. NMT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NMT_NEUCR

Accession

Primary (citable) accession number: Q7S3C8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	January 10, 2006
Last modified:	December 14, 2011
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program