Q7S1I0 (ATG26_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sterol 3-beta-glucosyltransferase
EC=2.4.1.173

Alternative name(s):
Autophagy-related protein 26

Gene names

Name:	atg-26
ORF Names:	NCU09301, NCU11419/NCU11407

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	1553 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May be involved in decane metabolism and autophagy. Involved in the biosynthesis of sterol glucoside By similarity.
Catalytic activity	UDP-glucose + a sterol = UDP + a sterol 3-beta-D-glucoside.
Subcellular location	Membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 28 family. Contains 3 GRAM domains. Contains 1 PH domain.
Sequence caution	The sequence EDO64866.1 differs from that shown. Reason: Translation C-terminally overlaps with the next contig. The coding sequence for this protein is split across two overlapping contigs in the current genome assembly. The sequence EDO65429.1 differs from that shown. Reason: Translation N-terminally overlaps with the next contig. The coding sequence for this protein is split across two overlapping contigs in the current genome assembly.

Ontologies

Keywords
Biological process	Autophagy Lipid synthesis Protein transport Steroid biosynthesis Sterol biosynthesis Transport
Cellular component	Membrane
Domain	Repeat
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid glycosylation Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sterol 3-beta-glucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1553	1553	Sterol 3-beta-glucosyltransferase		PRO_0000215613
Regions
	Domain	323 – 370	48	GRAM 1
	Domain	374 – 470	97	PH
	Domain	464 – 495	32	GRAM 2
	Domain	834 – 900	67	GRAM 3

Sequences

	Sequence		Length	Mass (Da)
	Q7S1I0 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 574FB8BAD1645240	FASTA	1,553	171,968
	10 20 30 40 50 60 MASSQPTSSG PILDSPPSPN PQSQLNTETS SSQHRANPSE APAQPGPGPA RPSALTKRPS 70 80 90 100 110 120 HREVAAYRMS RKLQRLRADS NQPHSPTMEL PDRLKDNGKE EDNEEDVLQP QGGMFMNMNQ 130 140 150 160 170 180 SIFGLIAAAG STVDFHDRFE GQSSEDEDDV PNHMAMTFAG PGIKGTARNR ETAGTVKSLS 190 200 210 220 230 240 QTVVFNKPPA SATVDAGPSN IHRRRLPGHK LLQSVPSLSR LSSSHKSKKT KQHNATAMAD 250 260 270 280 290 300 NKIEEEEDPD PSSPLPPLSK DETESLGLAP PIEIVRAEGN GAPLMSRMLE ARAEMEARPS 310 320 330 340 350 360 FDLDRLSGEH RRDDAGVTGQ LAKKLKDIFE FDTAEEVIEE YPCWLLQHVL LQGYMYITAN 370 380 390 400 410 420 HIAFYAHLPK KAHEIAKSGY LSKSGKRNPK YNRYWFRLKG DVLSYYQDPK DHYFPAGQID 430 440 450 460 470 480 LRYGISASVN DKEKEGNYFS VSTHHRTYHF KADSARSAKE WVKSLQRVIF RSHNDGDSVK 490 500 510 520 530 540 ISIPIRNILD IEEAQMVEFA DTCKIRVIDN DETYAIDEYF FSFFSFGKKA IQVLKVLVED 550 560 570 580 590 600 SSPEDSGAND APKGTGGDRA IGDNLGSPRT RTFSEGVKAT LSPVSPIQIA SPSSRASGDY 610 620 630 640 650 660 FKSSFDGTRP FSRRSFDASP GTAGYAGSPP RRLHGDGRRS FSKPRHEPHA STDSYAQSFD 670 680 690 700 710 720 DPSQASLSAL VASGSEDQSA SQILRGSDVF HSPIFRRSAS ATRATTEGEG VAAPPIVRQH 730 740 750 760 770 780 TAGLVRLHGP HHAATTGQIG DHMAEAEGGP STPMLQSIAM MGNYPLQRAN AFMGYLDQQS 790 800 810 820 830 840 RRMSNLLATE SMGYVEKVSG MWKGGKKHYD HPAGRRTERE DVEDDPEERA LSEARFQAHF 850 860 870 880 890 900 ALPETERLQA AYFGFIVRVL PLYGKIYISN RHFCFRSLLP GTRTKLILPL KDIETVDKEK 910 920 930 940 950 960 GFRFGYSGLV VVIRGHEEIF FEFAKAENRD DCAITIIQSL DAARYLAESQ EVEEAQAAEA 970 980 990 1000 1010 1020 ERDALNQARN EEFPDHEIEL PRQASGVSDA PTILFDDPKA SFLNFKPSEP MRITCLTIGS 1030 1040 1050 1060 1070 1080 RGDVQPYIAL CKRLLEEGHR PKIVTHREFK DWIESHGIEF GPVEGDPSEL MRICIENGTF 1090 1100 1110 1120 1130 1140 TYAFLREANS KFRGWLDELL TSAWEACQGS DLLIESPSAM AGIHIAEALG IPYFRAFTMP 1150 1160 1170 1180 1190 1200 WTRTRAYPHA FIMPGQKMGG AYNYITYVMF DNVFWKATAH QVNRWRNKYL GLPNTSLEKL 1210 1220 1230 1240 1250 1260 QPNKVPFLYN FSPSVVPPPI DYSDWIRVTG YWFLDEGGDK WQPPKELTDF IAKARADEKK 1270 1280 1290 1300 1310 1320 LVYIGFGSII VSDPAKMTQE IIDAVLKADV RCILSKGWSD RSATVDGVEK PKVADPSFPP 1330 1340 1350 1360 1370 1380 EILQIQSAPH DWLFQQVDAA AHHGGSGTTG ASLRAGIPTI IRPFFGDQFF FAGRVEDLGV 1390 1400 1410 1420 1430 1440 GIYLKKWGVQ SFARALWEAT HSSRMQMRAE VLGGQIRAEN GVDTAIQAIY RDLDYARNLI 1450 1460 1470 1480 1490 1500 TLKRQKHQSR RNSVATPTPG AKPNAPEDDQ GQAAEEDDID ADDEEEESWT FVGGNEDDLT 1510 1520 1530 1540 1550 GSMSMSRSDM LSQTVADLRG VKVGKAPALG SRVLSSPPTS PGAMRGAGGV KYV

References

[1]

"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AABX02000251 Genomic DNA. Translation: EDO64866.1. Sequence problems. AABX02000078 Genomic DNA. Translation: EDO65429.1. Sequence problems.
RefSeq	XP_001727957.1. XM_001727905.1. XP_001728520.1. XM_001728468.1.
3D structure databases
ProteinModelPortal	Q7S1I0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000009330; EFNCRP00000009312; EFNCRG00000009311. EFNCRT00000009857; EFNCRP00000009839; EFNCRG00000009838.
GeneID	5846980. 5847922.
KEGG	ncr:NCU11407. ncr:NCU11419.
NMPDR	fig|5141.1.peg.9926.
Phylogenomic databases
eggNOG	fuNOG07241.
GeneTree	EFGT00050000002910.
OrthoDB	EOG48KVK8.
Family and domain databases
InterPro	IPR004276. Glyco_trans_28. IPR004182. GRAM. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR002213. UDP_glucos_trans. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
KO	K05841.
PANTHER	PTHR11926. UDP_glucos_trans. 1 hit.
Pfam	PF03033. Glyco_transf_28. 1 hit. PF02893. GRAM. 2 hits. PF00169. PH. 1 hit. [Graphical view]
SMART	SM00568. GRAM. 2 hits. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG26_NEUCR

Accession

Primary (citable) accession number: Q7S1I0
Secondary accession number(s): A7UXD3, A7UXI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	December 15, 2003
Last modified:	December 14, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program