Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q7RZC1

- TRMB_NEUCR

UniProt

Q7RZC1 - TRMB_NEUCR

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trm8

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei105 – 1051S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei183 – 1831S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei186 – 1861UniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trm8
ORF Names:G17A4.290, NCU03936
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805: Chromosome 5, Linkage Group VI

Subcellular locationi

Nucleus UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 293293tRNA (guanine-N(7)-)-methyltransferasePRO_0000370600Add
BLAST

Interactioni

Subunit structurei

Forms a complex with trm82.UniRule annotation

Protein-protein interaction databases

STRINGi5141.NCU03936.1.

Structurei

3D structure databases

ProteinModelPortaliQ7RZC1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni128 – 1292S-adenosyl-L-methionine bindingUniRule annotation
Regioni163 – 1642S-adenosyl-L-methionine bindingUniRule annotation
Regioni261 – 2633S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000260965.
InParanoidiQ7RZC1.
KOiK03439.
OMAiRAHSNPI.
OrthoDBiEOG708W9T.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7RZC1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEPKNKRQK REDYRAALRA NGVTELPRKK FYRQRAHANP FSDHSLIYPP
60 70 80 90 100
TPEQMDWASL YPHYAVEEPI EQKTETTESE ESTGQELSAP AIRKLTKQVE
110 120 130 140 150
VADIGCGFGG LLIALAPVLP ETLVLGLEIR VSVTQFVEDR IKALRVQNEE
160 170 180 190 200
QKLYRNIAVL RANTMKFMPN FFNKGQLNKI FICFPDPHFK ARKHKQRIVS
210 220 230 240 250
TTLNSEYAYV LRPGGIVYTI TDVPDLHEWM VGHFNAHPAF ERVSVEEQEA
260 270 280 290
DPLVEIMRNE TEEGKKVTRH NGQKHVALFR RLEDPQWPED VSA
Length:293
Mass (Da):33,733
Last modified:December 15, 2003 - v1
Checksum:i1050E6C0BF85CC7D
GO

Sequence cautioni

The sequence CAF06164.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX908812 Genomic DNA. Translation: CAF06164.1. Sequence problems.
CM002241 Genomic DNA. Translation: EAA28339.1.
RefSeqiXP_957575.1. XM_952482.1.
UniGeneiNcr.21061.

Genome annotation databases

EnsemblFungiiEFNCRT00000003704; EFNCRP00000003704; EFNCRG00000003700.
GeneIDi3873713.
KEGGincr:NCU03936.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX908812 Genomic DNA. Translation: CAF06164.1 . Sequence problems.
CM002241 Genomic DNA. Translation: EAA28339.1 .
RefSeqi XP_957575.1. XM_952482.1.
UniGenei Ncr.21061.

3D structure databases

ProteinModelPortali Q7RZC1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5141.NCU03936.1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii EFNCRT00000003704 ; EFNCRP00000003704 ; EFNCRG00000003700 .
GeneIDi 3873713.
KEGGi ncr:NCU03936.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000260965.
InParanoidi Q7RZC1.
KOi K03439.
OMAi RAHSNPI.
OrthoDBi EOG708W9T.

Enzyme and pathway databases

UniPathwayi UPA00989 .

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
    Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
    Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  2. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Entry informationi

Entry nameiTRMB_NEUCR
AccessioniPrimary (citable) accession number: Q7RZC1
Secondary accession number(s): Q6M8Z9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: December 15, 2003
Last modified: October 29, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3