Reviewed, UniProtKB/Swiss-Prot Q7RYY1 (ATG15_NEUCR)

Names and origin

Protein names

Recommended name:
    Putative lipase atg-15
    EC=3.1.1.3
Alternative name(s):
    Autophagy-related protein 15

Gene names

Name:	atg-15
ORF Names:	NCU06436

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	553 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endosome Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 553	553	Putative lipase atg-15		PRO_0000317966
Regions
	Topological domain	1 – 18	18	Cytoplasmic By similarity
	Transmembrane	19 – 39	21	Signal-anchor for type II membrane protein
	Topological domain	40 – 553	514	Lumenal By similarity
Sites
	Active site	323	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	203	1	N-linked (GlcNAc...) Potential
	Glycosylation	225	1	N-linked (GlcNAc...) Potential
	Glycosylation	283	1	N-linked (GlcNAc...) Potential
	Glycosylation	307	1	N-linked (GlcNAc...) Potential
	Glycosylation	526	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7RYY1-1 [UniParc]. Last modified October 23, 2007. Version 2. Checksum: 6AC6A69C83E72C40	FASTA	553	61,132
	10 20 30 40 50 60 MLPSGKRKAD AFSCTSAARV TAKLALSFLA LSTTPLVNAF SYEEPNAQIV LPIDASPIKP 70 80 90 100 110 120 LLPEPPAPAE HKFTLRHIYH HGTYEHPTLH RKKDVPAQNA DVWLAADDEY GQERIGTLKA 130 140 150 160 170 180 RSSPVRIQRL ADRRPSVVDP MVAYARQQGY ASVLSPEAWT MDEVAGPDIT DKDTIISLAL 190 200 210 220 230 240 MAADAYVQTP DGADWEDVGA PFNRSLDFGW EGDGLRGHVF ADETNSTIVI GLKGTSVAVF 250 260 270 280 290 300 DGDGTTTNDK VNDNLFFSCC CAQQGPWTWH QVCDCATGTY SCNNTCVVQA LRQENRYYQA 310 320 330 340 350 360 GRELYANVTE LYPDANVWIV GHSLGGAMSS LLGLTYGDPV VTFEAVPEAL PAKRLGLPIP 370 380 390 400 410 420 PGSDPDAPQT REYTGAFHIG HTADPVYVGT CNGATATCAI GGYAMESACH TGRECVYDTV 430 440 450 460 470 480 GDLGWRVGIG THKIRVVISD VLRKYEKVPE CKFTPECRDC GNWKIICKYA NKWQGMVGMF 490 500 510 520 530 540 GRNHYHNWDG NHDDKRNAHN LYHYVPHSWV VRLQGQDYNL DRINNNNSYR DNSNNNNNKQ 550 YHMPNSRKVL GML

References

[1]

"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AABX02000027 Genomic DNA. Translation: EAA28091.2.
RefSeq	XP_957327.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q7RYY1.
Genome annotation databases
GeneID	3873496.
KEGG	ncr:NCU06436.
Enzyme and pathway databases
BRENDA	3.1.1.3. 266.
Family and domain databases
InterPro	IPR002921. Lipase_3. IPR008262. Lipase_Ser_AS. [Graphical view]
Pfam	PF01764. Lipase_3. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG15_NEUCR

Accession

Primary (citable) accession number: Q7RYY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 23, 2007
Last modified:	October 13, 2009
This is version 35 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)