Q7RXL1 (NCB5R_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-cytochrome b5 reductase 1
EC=1.6.2.2

Alternative name(s):
Microsomal cytochrome b reductase

Gene names

Name:	cbr-1
ORF Names:	NCU00216

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Electron donor reductase for cytochrome b5. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity. Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane helix
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	NADH-cytochrome b5 reductase 1		PRO_0000330160
Regions
	Transmembrane	33 – 50	18	Helical; Potential
	Domain	61 – 169	109	FAD-binding FR-type
	Nucleotide binding	149 – 164	16	FAD By similarity
	Nucleotide binding	175 – 209	35	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7RXL1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: AD00607089CF7B40	FASTA	310	34,001
	10 20 30 40 50 60 MSEESLLERK YFDGIYIPAG LLVFGTVIVK KEWTLYAALA ALILGAIKYF RMLPKKVLKP 70 80 90 100 110 120 AVFQEFELKE KTIISHNVAI YRFKLPTPDS VLGLPIGQHI SIGAAIEQPD GSTKEIVRSY 130 140 150 160 170 180 TPISGDHQPG YFDLLIKSYP TGNISKHMAS LQVGQTIRVK GPKGAFVYTP NMVRHFGMVA 190 200 210 220 230 240 GGTGITPMLQ VIRAIVRGRA AGDRTEVDLI FANVTAQDIL LKEDLDALVA QDKGIRVHYV 250 260 270 280 290 300 LDRPPEGWTG GVGFVTQEMV EKLLPKPASD VKILLCGPPP MISGLKKATE ALGFQKARPV 310 SKLEDQVFAF

References

[1]

"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AABX02000001 Genomic DNA. Translation: EAA27365.1.
RefSeq	XP_956601.1. XM_951508.2.
UniGene	Ncr.4248.
3D structure databases
HSSP	HSSP built from PDB template 1UMK based on UniProtKB P00387.
ProteinModelPortal	Q7RXL1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7RXL1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000000032; EFNCRP00000000032; EFNCRG00000000032.
GeneID	3872748.
KEGG	ncr:NCU00216.
NMPDR	fig|5141.1.peg.235.
Phylogenomic databases
eggNOG	fuNOG05226.
GeneTree	EFGT00050000001026.
OrthoDB	EOG4FFH9R.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
KO	K00360.
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCB5R_NEUCR

Accession

Primary (citable) accession number: Q7RXL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	December 15, 2003
Last modified:	December 14, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program