Reviewed, UniProtKB/Swiss-Prot Q7RVY5 (HEM1_NEUCR)

Names and origin

Protein names

Recommended name:
    5-aminolevulinate synthase, mitochondrial
    EC=2.3.1.37
Alternative name(s):
    5-aminolevulinic acid synthase
    Delta-aminolevulinate synthase
    Delta-ALA synthase

Gene names

Name:	hem-1
ORF Names:	NCU06189

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	629 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Heme biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Pyridoxal phosphate
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	5-aminolevulinate synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring nitrogenous groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 69	69	Mitochondrion Potential
	Chain	70 – 629	560	5-aminolevulinate synthase, mitochondrial		PRO_0000001242
Amino acid modifications
	Modified residue	391	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7RVY5-1 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: 01FA359E33FFEF00	FASTA	629	67,447
	10 20 30 40 50 60 MDSVLRQSKA VCPFMKKATA SSLRAMTTAA RPAASPCGGA ISKLQVLAHR CPVMGKAMAV 70 80 90 100 110 120 QSARTGGRAS AAPASFVHKA KLHTGRPREA QAVEGVFTGQ KAGLPPHPPV KPTTATAANP 130 140 150 160 170 180 SPAACTVSGN FGTKFNYEKF YENELEKKHK DKSYRYFNNI NRLAKDFPRA HMATKEEKVA 190 200 210 220 230 240 VWCANDYLGM GRNKHVLEAM HTTLDEYGAG AGGTRNISGH NKHAVELENS LAKLHATDAA 250 260 270 280 290 300 LVFSSCYVAN DATLATLGSK LPDCVILSDS LNHASMIQGI RHSGAKKIIF KHNDVEDLEK 310 320 330 340 350 360 KLAALPLHIP KIIAFESVYS MCGSIGPIEK FCDLAEKYGA ITFNDEVHAV GMYGPHGAGV 370 380 390 400 410 420 AEHLDWEAHQ RGETKGTIAD RIDIYSGTLG KAYGCVGGYI AGSAKLVDTI RSLAPGFIFT 430 440 450 460 470 480 TTLPPAVMAG ARAAIEYQMS YDGDRRLQQL HTRAVKEALA DRDIPVIPNP SHIIPILVGN 490 500 510 520 530 540 AELAKKASDK LLNDHQIYVQ SINYPTVPVG QERLRITPTP GHTKQFRDHL VAALDSIWTE 550 560 570 580 590 600 LGIKRTSDWA AEGGFIGVGE AEAEPVAPLW TDEQLGIADA VAELRAQASD EKKGVINQLL 610 620 ESVALEQERE ALNAAEEVAD AAKAAAASA

References

[1]

"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AABX02000025 Genomic DNA. Translation: EAA33765.2.
RefSeq	XP_963001.2.
3D structure databases
SMR	Q7RVY5. Positions 136-538.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7RVY5.
Genome annotation databases
GeneID	3879149.
KEGG	ncr:NCU06189.
Phylogenomic databases
eggNOG	fuNOG07159.
OrthoDB	EOG9CVHQ2.
PhylomeDB	Q7RVY5.
Enzyme and pathway databases
BRENDA	2.3.1.37. 266.
Family and domain databases
InterPro	IPR010961. 4pyrrol_synth_NH2levulA_synth. IPR001917. Aminotrans_II_pyridoxalP_BS. IPR004839. Aminotransferase_I/II. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 2 hits.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01821. 5aminolev_synth. 1 hit.
PROSITE	PS00599. AA_TRANSFER_CLASS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM1_NEUCR

Accession

Primary (citable) accession number: Q7RVY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	December 4, 2007
Last modified:	February 9, 2010
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)