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Protein

Cyanide hydratase

Gene

cnh-1

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydration of cyanide to formamide. Degradation of cyanide may be important for plant pathogenic fungi in infection of cyanogenic plants.UniRule annotation1 Publication

Catalytic activityi

Formamide = cyanide + H2O.UniRule annotation1 Publication

pH dependencei

Optimum pH is 6-7. Active fom pH 5 to pH 9.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei46Proton acceptorPROSITE-ProRule annotation1
Active sitei128PROSITE-ProRule annotation1
Active sitei163NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase

Names & Taxonomyi

Protein namesi
Recommended name:
Cyanide hydratase1 PublicationUniRule annotation (EC:4.2.1.66UniRule annotation1 Publication)
Short name:
CHT1 PublicationUniRule annotation
Alternative name(s):
Cyanide-degrading nitrilaseUniRule annotation
Formamide hydrolyaseUniRule annotation
Gene namesi
Name:cnh-11 Publication
Synonyms:cht1 Publication
ORF Names:NCU04697
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 5, Linkage Group VI

Organism-specific databases

EuPathDBiFungiDB:NCU04697.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004400351 – 351Cyanide hydrataseAdd BLAST351

Expressioni

Inductioni

By cyanide.UniRule annotation1 Publication

Interactioni

Subunit structurei

Oligomer of dimers, forming left-handed helical fibers with a diameter of 13 nm but with lengths ranging from approximately 1 um at the leading edge of the peak to having approximately the same length and diameter at the trailing edge.UniRule annotation1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ7RVT0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 285CN hydrolasePROSITE-ProRule annotationAdd BLAST280

Sequence similaritiesi

Belongs to the carbon-nitrogen hydrolase superfamily. Nitrilase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000256364.
InParanoidiQ7RVT0.
KOiK10675.
OMAiNGHARIY.
OrthoDBiEOG092C351H.

Family and domain databases

Gene3Di3.60.110.10. 1 hit.
HAMAPiMF_03224. CN_hydrolase. 1 hit.
InterProiView protein in InterPro
IPR003010. C-N_Hydrolase.
IPR036526. C-N_Hydrolase_sf.
IPR000132. Nitrilase/CN_hydratase_CS.
PfamiView protein in Pfam
PF00795. CN_hydrolase. 1 hit.
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiView protein in PROSITE
PS50263. CN_HYDROLASE. 1 hit.
PS00920. NITRIL_CHT_1. 1 hit.
PS00921. NITRIL_CHT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7RVT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLTKYKAAA VTSEPCWFDL EGGVRKTIDF INEAGQAGCK LVAFPEVWIP
60 70 80 90 100
GYPYWMWKVT YQQSLPMLKK YRENAMAVDS DEFRRIRRAA RDNQIYVSLG
110 120 130 140 150
FAEIDHATLY LAQALIDPTG EVINHRRKIK PTHVEKLVYG DGAGDTFMSV
160 170 180 190 200
TPTELGRLGQ LNCWENMNPF LKSLNVSMGE QIHIAAWPIY PGKETLKYPD
210 220 230 240 250
PATNVADPAS DLVTPAYAIE TGTWTLAPFQ RLSVEGLKKN TPEGVEPETD
260 270 280 290 300
PSTYNGHARI YRPDGSLVVR PDKDFDGLLF VDIDLNECHL TKALADFAGH
310 320 330 340 350
YMRPDLIRLL VDTSRKELVT EVDRNGGIVQ YSTRERLGLN TPLENDKEGK

K
Length:351
Mass (Da):39,526
Last modified:October 23, 2007 - v2
Checksum:i52355FCBD1F134BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM002241 Genomic DNA. Translation: EAA30924.2.
RefSeqiXP_960160.2. XM_955067.3.

Genome annotation databases

EnsemblFungiiEAA30924; EAA30924; NCU04697.
GeneIDi3876307.
KEGGincr:NCU04697.

Similar proteinsi

Entry informationi

Entry nameiCHT_NEUCR
AccessioniPrimary (citable) accession number: Q7RVT0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2017
Last sequence update: October 23, 2007
Last modified: October 25, 2017
This is version 71 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families