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Protein

Pyruvate kinase

Gene

ace-8

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei62 – 621SubstrateBy similarity
Metal bindingi64 – 641PotassiumBy similarity
Metal bindingi66 – 661PotassiumBy similarity
Metal bindingi97 – 971PotassiumBy similarity
Metal bindingi98 – 981Potassium; via carbonyl oxygenBy similarity
Sitei253 – 2531Transition state stabilizerBy similarity
Metal bindingi255 – 2551MagnesiumSequence Analysis
Binding sitei278 – 2781Substrate; via amide nitrogenBy similarity
Metal bindingi279 – 2791MagnesiumBy similarity
Binding sitei279 – 2791Substrate; via amide nitrogenBy similarity
Binding sitei311 – 3111SubstrateBy similarity
Binding sitei350 – 3501ADPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Alternative name(s):
Acetate-requiring protein 8
Gene namesi
Name:ace-8
Synonyms:pyk1
ORF Names:NCU06075
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805: Chromosome 7, Linkage Group VII

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 527527Pyruvate kinasePRO_0000112115Add
BLAST

Proteomic databases

PRIDEiQ7RVA8.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi5141.NCU06075.1.

Structurei

3D structure databases

ProteinModelPortaliQ7RVA8.
SMRiQ7RVA8. Positions 30-514.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021559.
InParanoidiQ7RVA8.
KOiK00873.
OMAiISKKCAY.
OrthoDBiEOG7M6DJC.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7RVA8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAATALDHLS LGGKIQWLAQ LNTEFQPARE FRRTSIICTI GPKTNSVEAI
60 70 80 90 100
NKLRDAGLNV VRMNFSHGSY EYHQSVIDNA RQAEKVHPGR PIAIALDTKG
110 120 130 140 150
PEIRTGNTKN DEDIPISAGT ILNITTDEKY KDECTIEHMY VDYVNITKVI
160 170 180 190 200
APGRIIYVDD GVLAFEVLEI VDDKTIKVKA RNNGYISSRK GVNLPNTDVD
210 220 230 240 250
LPALSEKDKA DLRFGVKNKV DMVFASFIRR GQDIKDIREV LGEDGKQIQI
260 270 280 290 300
IAKIENRQGL NNFAEILAET DGVMVARGDL GIEIPAAEVF AAQKKIIAMC
310 320 330 340 350
NIAGKPVICA TQMLESMIKN PRPTRAEISD VGNAVTDGAD CVMLSGETAK
360 370 380 390 400
GAYPTEAVRE MSEAVLKAEN TIPYVSHFEE LCSLAKRPVS IVESCAMASV
410 420 430 440 450
RASLDLNAAA ILVLSTSGES ARLISKYRPV CPIIMITRND SASRYAHLYR
460 470 480 490 500
GVYPFLFPES KPDFSKVNWQ EDVDRRIKWG LSHGIGLKVL NEGETVVVVQ
510 520
GWKGGMGNTN TFRIVKADVN HLGLGQP
Length:527
Mass (Da):57,870
Last modified:December 15, 2003 - v1
Checksum:i0193D192F0CBA18B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM002242 Genomic DNA. Translation: ESA41917.1.
CM002242 Genomic DNA. Translation: ESA41918.1.
RefSeqiXP_001728254.1. XM_001728202.1.
XP_959838.1. XM_954745.2.
UniGeneiNcr.20447.

Genome annotation databases

EnsemblFungiiEFNCRT00000005490; EFNCRP00000005484; EFNCRG00000005481.
EFNCRT00000005491; EFNCRP00000005485; EFNCRG00000005481.
GeneIDi3875985.
KEGGincr:NCU06075.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM002242 Genomic DNA. Translation: ESA41917.1.
CM002242 Genomic DNA. Translation: ESA41918.1.
RefSeqiXP_001728254.1. XM_001728202.1.
XP_959838.1. XM_954745.2.
UniGeneiNcr.20447.

3D structure databases

ProteinModelPortaliQ7RVA8.
SMRiQ7RVA8. Positions 30-514.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5141.NCU06075.1.

Proteomic databases

PRIDEiQ7RVA8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEFNCRT00000005490; EFNCRP00000005484; EFNCRG00000005481.
EFNCRT00000005491; EFNCRP00000005485; EFNCRG00000005481.
GeneIDi3875985.
KEGGincr:NCU06075.

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021559.
InParanoidiQ7RVA8.
KOiK00873.
OMAiISKKCAY.
OrthoDBiEOG7M6DJC.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Entry informationi

Entry nameiKPYK_NEUCR
AccessioniPrimary (citable) accession number: Q7RVA8
Secondary accession number(s): A7UWL6, V5IKJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: December 15, 2003
Last modified: January 7, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.