Reviewed, UniProtKB/Swiss-Prot Q7RVA8 (KPYK_NEUCR)

Names and origin

Protein names

Recommended name:
    Pyruvate kinase
      Short name=PK
    EC=2.7.1.40

Gene names

Name:	pyk-1
ORF Names:	NCU06075

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium By similarity. Potassium By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Pyruvate
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 527	527	Pyruvate kinase		PRO_0000112115
Sites
	Active site	253	1	By similarity
	Metal binding	255	1	Magnesium Potential
	Metal binding	276	1	Magnesium Potential
	Metal binding	277	1	Magnesium Potential
	Binding site	350	1	ADP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7RVA8-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 0193D192F0CBA18B	FASTA	527	57,870
	10 20 30 40 50 60 MAATALDHLS LGGKIQWLAQ LNTEFQPARE FRRTSIICTI GPKTNSVEAI NKLRDAGLNV 70 80 90 100 110 120 VRMNFSHGSY EYHQSVIDNA RQAEKVHPGR PIAIALDTKG PEIRTGNTKN DEDIPISAGT 130 140 150 160 170 180 ILNITTDEKY KDECTIEHMY VDYVNITKVI APGRIIYVDD GVLAFEVLEI VDDKTIKVKA 190 200 210 220 230 240 RNNGYISSRK GVNLPNTDVD LPALSEKDKA DLRFGVKNKV DMVFASFIRR GQDIKDIREV 250 260 270 280 290 300 LGEDGKQIQI IAKIENRQGL NNFAEILAET DGVMVARGDL GIEIPAAEVF AAQKKIIAMC 310 320 330 340 350 360 NIAGKPVICA TQMLESMIKN PRPTRAEISD VGNAVTDGAD CVMLSGETAK GAYPTEAVRE 370 380 390 400 410 420 MSEAVLKAEN TIPYVSHFEE LCSLAKRPVS IVESCAMASV RASLDLNAAA ILVLSTSGES 430 440 450 460 470 480 ARLISKYRPV CPIIMITRND SASRYAHLYR GVYPFLFPES KPDFSKVNWQ EDVDRRIKWG 490 500 510 520 LSHGIGLKVL NEGETVVVVQ GWKGGMGNTN TFRIVKADVN HLGLGQP

References

[1]

"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AABX02000021 Genomic DNA. Translation: EAA30602.1.
RefSeq	XP_959838.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3875985.
NMPDR	fig|5141.1.peg.3863.
Enzyme and pathway databases
BRENDA	2.7.1.40. 266.
Family and domain databases
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit. G3DSA:3.40.1380.20. Pyrv_Knase_a/b. 1 hit.
PANTHER	PTHR11817. Pyruvate_kinase. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
ProDom	PD001009. Pyruvate_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_NEUCR

Accession

Primary (citable) accession number: Q7RVA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	December 15, 2003
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)