Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q7RTX7 (CTSR4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cation channel sperm-associated protein 4

Short name=CatSper4
Gene names
Name:CATSPER4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length472 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Voltage-gated calcium channel that plays a central role in calcium-dependent physiological responses essential for successful fertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the oocyte. Activated by extracellular progesterone and prostaglandins following the sequence: progesterone > PGF1-alpha = PGE1 > PGA1 > PGE2 >> PGD2. The primary effect of progesterone activation is to shift voltage dependence towards more physiological, negative membrane potentials; it is not mediated by metabotropic receptors and second messengers. Sperm capacitation enhances the effect of progesterone by providing additional negative shift. Also activated by the elevation of intracellular pH. Ref.5 Ref.6

Subunit structure

Heterotetramer; possibly composed of CATSPER1, CATSPER2, CATSPER3 and CATSPER4 Potential. Component of the CatSper complex. Interacts with CATSPER1 By similarity.

Subcellular location

Cell projectionciliumflagellum membrane; Multi-pass membrane protein. Note: Specifically located in the principal piece of sperm tail.

Tissue specificity

Testis-specific. Ref.3 Ref.4

Sequence similarities

Belongs to the cation channel sperm-associated (TC 1.A.1.19) family. [View classification]

Sequence caution

The sequence AAI28139.1 differs from that shown. Reason: Frameshift at position 21.

The sequence CAH71538.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processCalcium transport
Differentiation
Ion transport
Spermatogenesis
Transport
   Cellular componentCell membrane
Cell projection
Cilium
Flagellum
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainTransmembrane
Transmembrane helix
   LigandCalcium
   Molecular functionCalcium channel
Developmental protein
Ion channel
Voltage-gated channel
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

ciliary or bacterial-type flagellar motility

Inferred from electronic annotation. Source: Ensembl

multicellular organism reproduction

Traceable author statement. Source: Reactome

multicellular organismal development

Inferred from electronic annotation. Source: UniProtKB-KW

single fertilization

Traceable author statement. Source: Reactome

sodium ion transport

Inferred from electronic annotation. Source: Ensembl

sperm motility

Inferred from electronic annotation. Source: Ensembl

sperm-egg recognition

Traceable author statement. Source: Reactome

spermatogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentCatSper complex

Inferred from sequence or structural similarity. Source: UniProtKB

acrosomal vesicle

Inferred from electronic annotation. Source: Ensembl

motile cilium

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functioncalcium activated cation channel activity

Inferred from electronic annotation. Source: Ensembl

calcium channel activity

Inferred from electronic annotation. Source: UniProtKB-KW

voltage-gated ion channel activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q7RTX7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q7RTX7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     227-242: Missing.
     401-472: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 472472Cation channel sperm-associated protein 4
PRO_0000295681

Regions

Topological domain1 – 9090Cytoplasmic Potential
Transmembrane91 – 11121Helical; Name=Segment S1; Potential
Topological domain112 – 13019Extracellular Potential
Transmembrane131 – 15121Helical; Name=Segment S2; Potential
Topological domain152 – 1576Cytoplasmic Potential
Transmembrane158 – 17821Helical; Name=Segment S3; Potential
Topological domain1791Extracellular Potential
Transmembrane180 – 19718Helical; Name=Segment S4; Potential
Topological domain198 – 21619Cytoplasmic Potential
Transmembrane217 – 23721Helical; Name=Segment S5; Potential
Topological domain238 – 2469Extracellular Potential
Intramembrane247 – 26721Helical; Pore-forming; Potential
Topological domain268 – 28417Extracellular Potential
Transmembrane285 – 30521Helical; Name=Segment S6; Potential
Topological domain306 – 472167Cytoplasmic Potential

Natural variations

Alternative sequence227 – 24216Missing in isoform 2.
VSP_026979
Alternative sequence401 – 47272Missing in isoform 2.
VSP_026980
Natural variant771Q → R.
Corresponds to variant rs11247866 [ dbSNP | Ensembl ].
VAR_033310
Natural variant1241L → F.
Corresponds to variant rs12138368 [ dbSNP | Ensembl ].
VAR_033311
Natural variant2931I → V.
Corresponds to variant rs17257155 [ dbSNP | Ensembl ].
VAR_033312
Natural variant4361D → N.
Corresponds to variant rs6657616 [ dbSNP | Ensembl ].
VAR_033313

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: 5AC94CD0971A25FE

FASTA47254,092
        10         20         30         40         50         60 
MRDNEKAWWQ QWTSHTGLEG WGGTQEDRMG FGGAVAALRG RPSPLQSTIH ESYGRPEEQV 

        70         80         90        100        110        120 
LINRQEITNK ADAWDMQEFI THMYIKQLLR HPAFQLLLAL LLVINAITIA LRTNSYLDQK 

       130        140        150        160        170        180 
HYELFSTIDD IVLTILLCEV LLGWLNGFWI FWKDGWNILN FIIVFILLLR FFINEINIPS 

       190        200        210        220        230        240 
INYTLRALRL VHVCMAVEPL ARIIRVILQS VPDMANIMVL ILFFMLVFSV FGVTLFGAFV 

       250        260        270        280        290        300 
PKHFQNIQVA LYTLFICITQ DGWVDIYSDF QTEKREYAME IGGAIYFTIF ITIGAFIGIN 

       310        320        330        340        350        360 
LFVIVVTTNL EQMMKAGEQG QQQRITFSET GAEEEEENDQ LPLVHCVVAR SEKSGLLQEP 

       370        380        390        400        410        420 
LAGGPLSNLS ENTCDNFCLV LEAIQENLRQ YKEIRDELNM IVEEVRAIRF NQEQESEVLN 

       430        440        450        460        470 
RRSSTSGSLE TTSSKDIRQM SQQQDLLSAL VSMEKVHDSS SQILLKKHKS SH 

« Hide

Isoform 2 [UniParc].

Checksum: 00D375891B88F6F3
Show »

FASTA38444,229

References

« Hide 'large scale' references
[1]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
[3]"Identification of human and mouse CatSper3 and CatSper4 genes: characterisation of a common interaction domain and evidence for expression in testis."
Lobley A., Pierron V., Reynolds L., Allen L., Michalovich D.
Reprod. Biol. Endocrinol. 1:53-53(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
[4]"Expression of CatSper family transcripts in the mouse testis during post-natal development and human ejaculated spermatozoa: relationship to sperm motility."
Li H.-G., Ding X.-F., Liao A.-H., Kong X.-B., Xiong C.-L.
Mol. Hum. Reprod. 13:299-306(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"The CatSper channel mediates progesterone-induced Ca2+ influx in human sperm."
Strunker T., Goodwin N., Brenker C., Kashikar N.D., Weyand I., Seifert R., Kaupp U.B.
Nature 471:382-386(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN ACTIVATION BY PROGESTERONE.
[6]"Progesterone activates the principal Ca2+ channel of human sperm."
Lishko P.V., Botchkina I.L., Kirichok Y.
Nature 471:387-391(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN ACTIVATION BY PROGESTERONE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL355877 Genomic DNA. Translation: CAH71538.1. Different initiation.
BC128138 mRNA. Translation: AAI28139.1. Frameshift.
BC128139 mRNA. Translation: AAI28140.1.
BN000273 mRNA. Translation: CAE30475.1.
RefSeqNP_937770.1. NM_198137.1.
UniGeneHs.123532.

3D structure databases

ProteinModelPortalQ7RTX7.
SMRQ7RTX7. Positions 94-335.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000341006.

Chemistry

GuidetoPHARMACOLOGY391.

Protein family/group databases

TCDB1.A.1.19.1. the voltage-gated ion channel (vic) superfamily.

PTM databases

PhosphoSiteQ7RTX7.

Polymorphism databases

DMDM74713153.

Proteomic databases

PaxDbQ7RTX7.
PRIDEQ7RTX7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000456354; ENSP00000390423; ENSG00000188782. [Q7RTX7-1]
ENST00000518899; ENSP00000429464; ENSG00000188782. [Q7RTX7-2]
GeneID378807.
KEGGhsa:378807.
UCSCuc010oez.2. human. [Q7RTX7-1]

Organism-specific databases

CTD378807.
GeneCardsGC01P026517.
HGNCHGNC:23220. CATSPER4.
HPAHPA045257.
MIM609121. gene.
neXtProtNX_Q7RTX7.
PharmGKBPA134941696.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1226.
HOGENOMHOG000112053.
HOVERGENHBG102124.
InParanoidQ7RTX7.
KOK16892.
PhylomeDBQ7RTX7.
TreeFamTF343841.

Enzyme and pathway databases

ReactomeREACT_163848. Reproduction.

Gene expression databases

BgeeQ7RTX7.
CleanExHS_CATSPER4.
GenevestigatorQ7RTX7.

Family and domain databases

InterProIPR028744. CatSper4.
IPR005821. Ion_trans_dom.
[Graphical view]
PANTHERPTHR10037:SF8. PTHR10037:SF8. 1 hit.
PfamPF00520. Ion_trans. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiCatSper4.
GenomeRNAi378807.
NextBio100804.
PROQ7RTX7.
SOURCESearch...

Entry information

Entry nameCTSR4_HUMAN
AccessionPrimary (citable) accession number: Q7RTX7
Secondary accession number(s): A1A4W6, Q5VY71
Entry history
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: December 15, 2003
Last modified: April 16, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM