ID   TS1R1_HUMAN             Reviewed;         841 AA.
AC   Q7RTX1; B2RMX0; Q5SY22; Q5SY24; Q8NGZ7; Q8TDJ7; Q8TDJ8; Q8TDJ9; Q8TDK0;
DT   04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   24-JAN-2024, entry version 170.
DE   RecName: Full=Taste receptor type 1 member 1;
DE   AltName: Full=G-protein coupled receptor 70;
DE   Flags: Precursor;
GN   Name=TAS1R1; Synonyms=GPR70, T1R1, TR1; ORFNames=GM148;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S.,
RA   Tsutsumi S., Aburatani H., Asai K., Akiyama Y.;
RT   "Genome-wide discovery and analysis of human seven transmembrane helix
RT   receptor genes.";
RL   Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16710414; DOI=10.1038/nature04727;
RA   Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA   Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA   Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA   Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA   Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA   Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA   Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA   Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA   Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA   Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA   Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA   Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA   Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA   Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA   Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA   Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA   Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA   Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA   Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA   McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA   Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA   Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA   Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA   Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA   Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA   White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA   Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA   Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA   Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT   "The DNA sequence and biological annotation of human chromosome 1.";
RL   Nature 441:315-321(2006).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT GLU-347.
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 65-841 (ISOFORMS 1; 2; 3 AND 4), AND VARIANT
RP   GLU-347.
RX   PubMed=11891061; DOI=10.1016/s0378-1119(01)00897-6;
RA   Makalowska I., Sood R., Faruque M.U., Hu P., Robbins C.M., Eddings E.M.,
RA   Mestre J.D., Baxevanis A.D., Carpten J.D.;
RT   "Identification of six novel genes by experimental validation of
RT   GeneMachine predicted genes.";
RL   Gene 284:203-213(2002).
RN   [5]
RP   IDENTIFICATION (ISOFORM 1), AND FUNCTION.
RX   PubMed=11917125; DOI=10.1073/pnas.072090199;
RA   Li X., Staszewski L., Xu H., Durick K., Zoller M., Adler E.;
RT   "Human receptors for sweet and umami taste.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:4692-4696(2002).
CC   -!- FUNCTION: Putative taste receptor. TAS1R1/TAS1R3 responds to the umami
CC       taste stimulus (the taste of monosodium glutamate). Sequence
CC       differences within and between species can significantly influence the
CC       selectivity and specificity of taste responses.
CC       {ECO:0000269|PubMed:11917125}.
CC   -!- SUBUNIT: Forms heterodimers with TAS1R3.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=4;
CC       Name=1; Synonyms=Gm148 form B;
CC         IsoId=Q7RTX1-1; Sequence=Displayed;
CC       Name=2; Synonyms=Gm148 form C;
CC         IsoId=Q7RTX1-2; Sequence=VSP_012418;
CC       Name=3; Synonyms=Gm148 form A;
CC         IsoId=Q7RTX1-3; Sequence=VSP_012418, VSP_012420;
CC       Name=4; Synonyms=Gm148 form D;
CC         IsoId=Q7RTX1-4; Sequence=VSP_012418, VSP_012421, VSP_012422;
CC   -!- MISCELLANEOUS: [Isoform 4]: Incomplete sequence. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor 3 family. TAS1R
CC       subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAL91359.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=AAL91360.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=AAL91361.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=BAC05845.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=AAL91358.1; Type=Frameshift; Evidence={ECO:0000305};
CC   -!- WEB RESOURCE: Name=Protein Spotlight; Note=The taste experience - Issue
CC       55 of February 2005;
CC       URL="https://web.expasy.org/spotlight/back_issues/055";
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DR   EMBL; AB065618; BAC05845.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL591866; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC136515; AAI36516.1; -; mRNA.
DR   EMBL; BC136516; AAI36517.1; -; mRNA.
DR   EMBL; AF387617; AAL91358.1; ALT_SEQ; mRNA.
DR   EMBL; AF387618; AAL91359.1; ALT_INIT; mRNA.
DR   EMBL; AF387619; AAL91360.1; ALT_INIT; mRNA.
DR   EMBL; AF387620; AAL91361.1; ALT_INIT; mRNA.
DR   EMBL; BK000153; DAA00012.1; -; mRNA.
DR   CCDS; CCDS81.1; -. [Q7RTX1-1]
DR   CCDS; CCDS82.1; -. [Q7RTX1-2]
DR   RefSeq; NP_619642.2; NM_138697.3. [Q7RTX1-1]
DR   RefSeq; NP_803884.1; NM_177540.2. [Q7RTX1-2]
DR   AlphaFoldDB; Q7RTX1; -.
DR   SMR; Q7RTX1; -.
DR   BioGRID; 123328; 4.
DR   STRING; 9606.ENSP00000331867; -.
DR   BindingDB; Q7RTX1; -.
DR   ChEMBL; CHEMBL3832641; -.
DR   TCDB; 9.A.14.7.5; the g-protein-coupled receptor (gpcr) family.
DR   GlyCosmos; Q7RTX1; 6 sites, No reported glycans.
DR   GlyGen; Q7RTX1; 6 sites.
DR   iPTMnet; Q7RTX1; -.
DR   PhosphoSitePlus; Q7RTX1; -.
DR   BioMuta; TAS1R1; -.
DR   DMDM; 57013075; -.
DR   EPD; Q7RTX1; -.
DR   MassIVE; Q7RTX1; -.
DR   PaxDb; 9606-ENSP00000331867; -.
DR   PeptideAtlas; Q7RTX1; -.
DR   Antibodypedia; 27421; 264 antibodies from 26 providers.
DR   DNASU; 80835; -.
DR   Ensembl; ENST00000333172.11; ENSP00000331867.6; ENSG00000173662.21. [Q7RTX1-1]
DR   Ensembl; ENST00000351136.7; ENSP00000312558.5; ENSG00000173662.21. [Q7RTX1-2]
DR   GeneID; 80835; -.
DR   KEGG; hsa:80835; -.
DR   MANE-Select; ENST00000333172.11; ENSP00000331867.6; NM_138697.4; NP_619642.2.
DR   UCSC; uc001ant.4; human. [Q7RTX1-1]
DR   AGR; HGNC:14448; -.
DR   CTD; 80835; -.
DR   DisGeNET; 80835; -.
DR   GeneCards; TAS1R1; -.
DR   HGNC; HGNC:14448; TAS1R1.
DR   HPA; ENSG00000173662; Tissue enriched (testis).
DR   MIM; 606225; gene.
DR   neXtProt; NX_Q7RTX1; -.
DR   OpenTargets; ENSG00000173662; -.
DR   PharmGKB; PA37882; -.
DR   VEuPathDB; HostDB:ENSG00000173662; -.
DR   eggNOG; KOG1056; Eukaryota.
DR   GeneTree; ENSGT00940000161264; -.
DR   HOGENOM; CLU_005389_5_1_1; -.
DR   InParanoid; Q7RTX1; -.
DR   OMA; EDWAIST; -.
DR   OrthoDB; 4096532at2759; -.
DR   PhylomeDB; Q7RTX1; -.
DR   TreeFam; TF331269; -.
DR   PathwayCommons; Q7RTX1; -.
DR   Reactome; R-HSA-418594; G alpha (i) signalling events.
DR   Reactome; R-HSA-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR   Reactome; R-HSA-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR   BioGRID-ORCS; 80835; 13 hits in 1142 CRISPR screens.
DR   ChiTaRS; TAS1R1; human.
DR   GeneWiki; TAS1R1; -.
DR   GenomeRNAi; 80835; -.
DR   Pharos; Q7RTX1; Tbio.
DR   PRO; PR:Q7RTX1; -.
DR   Proteomes; UP000005640; Chromosome 1.
DR   RNAct; Q7RTX1; Protein.
DR   Bgee; ENSG00000173662; Expressed in primordial germ cell in gonad and 83 other cell types or tissues.
DR   ExpressionAtlas; Q7RTX1; baseline and differential.
DR   GO; GO:0016020; C:membrane; IC:UniProtKB.
DR   GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR   GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR   GO; GO:0008527; F:taste receptor activity; IPI:UniProtKB.
DR   GO; GO:0050917; P:sensory perception of umami taste; IDA:UniProtKB.
DR   CDD; cd06363; PBP1_taste_receptor; 1.
DR   Gene3D; 3.40.50.2300; -; 2.
DR   Gene3D; 2.10.50.30; GPCR, family 3, nine cysteines domain; 1.
DR   InterPro; IPR001828; ANF_lig-bd_rcpt.
DR   InterPro; IPR000337; GPCR_3.
DR   InterPro; IPR011500; GPCR_3_9-Cys_dom.
DR   InterPro; IPR038550; GPCR_3_9-Cys_sf.
DR   InterPro; IPR017978; GPCR_3_C.
DR   InterPro; IPR000068; GPCR_3_Ca_sens_rcpt-rel.
DR   InterPro; IPR017979; GPCR_3_CS.
DR   InterPro; IPR028082; Peripla_BP_I.
DR   PANTHER; PTHR24061; CALCIUM-SENSING RECEPTOR-RELATED; 1.
DR   PANTHER; PTHR24061:SF3; TASTE RECEPTOR TYPE 1 MEMBER 1; 1.
DR   Pfam; PF00003; 7tm_3; 1.
DR   Pfam; PF01094; ANF_receptor; 1.
DR   Pfam; PF07562; NCD3G; 1.
DR   PRINTS; PR00592; CASENSINGR.
DR   PRINTS; PR00248; GPCRMGR.
DR   SUPFAM; SSF53822; Periplasmic binding protein-like I; 1.
DR   PROSITE; PS00980; G_PROTEIN_RECEP_F3_2; 1.
DR   PROSITE; PS00981; G_PROTEIN_RECEP_F3_3; 1.
DR   PROSITE; PS50259; G_PROTEIN_RECEP_F3_4; 1.
DR   Genevisible; Q7RTX1; HS.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Cell membrane; G-protein coupled receptor;
KW   Glycoprotein; Membrane; Receptor; Reference proteome; Sensory transduction;
KW   Signal; Taste; Transducer; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..841
FT                   /note="Taste receptor type 1 member 1"
FT                   /id="PRO_0000012954"
FT   TOPO_DOM        21..567
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        568..588
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        589..603
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        604..624
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        625..639
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        640..660
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        661..680
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        681..701
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        702..725
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        726..746
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        747..761
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        762..782
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        783..795
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        796..816
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        817..841
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        87
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        88
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        95
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        291
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        479
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        529
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         167..420
FT                   /note="Missing (in isoform 2, isoform 3 and isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:11891061"
FT                   /id="VSP_012418"
FT   VAR_SEQ         421..841
FT                   /note="LLEQIHKVHFLLHKDTVAFNDNRDPLSSYNIIAWDWNGPKWTFTVLGSSTWS
FT                   PVQLNINETKIQWHGKDNQVPKSVCSSDCLEGHQRVVTGFHHCCFECVPCGAGTFLNKS
FT                   DLYRCQPCGKEEWAPEGSQTCFPRTVVFLALREHTSWVLLAANTLLLLLLLGTAGLFAW
FT                   HLDTPVVRSAGGRLCFLMLGSLAAGSGSLYGFFGEPTRPACLLRQALFALGFTIFLSCL
FT                   TVRSFQLIIIFKFSTKVPTFYHAWVQNHGAGLFVMISSAAQLLICLTWLVVWTPLPARE
FT                   YQRFPHLVMLECTETNSLGFILAFLYNGLLSISAFACSYLGKDLPENYNEAKCVTFSLL
FT                   FNFVSWIAFFTTASVYDGKYLPAANMMAGLSSLSSGFGGYFLPKCYVILCRPDLNSTEH
FT                   FQASIQDYTRRCGST -> LSYAASSETLSVKRQYPSFLRTIPNDKYQVETMVLLLQKF
FT                   GWTWISLVGSSDDYGQLGVQALENQATGQGICIAFKDIMPFSAQVGDERMQCLMRHLAQ
FT                   AGATVVVVFSSRQLARVFFESVVLTNLTGKVWVASEAWALSRHITGVPGIQRIGMVLGV
FT                   AIQKRAVPGLKAFEEAYARADKKAPRPCHKGSWCSSNQLCRECQAFMAHTMPKLKAFSM
FT                   SSAYNAYRAVYAVAHGLHQLLGCASGACSRGRVYPWQTSTDASLVGKKSGHLREARPAS
FT                   RALWCFWLCVSTPLGCCWQLTRCCCCCCLGLLACLPGT (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:11891061"
FT                   /id="VSP_012420"
FT   VAR_SEQ         532..574
FT                   /note="DLYRCQPCGKEEWAPEGSQTCFPRTVVFLALREHTSWVLLAAN -> ATWVR
FT                   TCQRTTTRTNVSPSACSSTSCPGSPSSPRPASTTAXTC (in isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:11891061"
FT                   /id="VSP_012421"
FT   VAR_SEQ         575..841
FT                   /note="Missing (in isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:11891061"
FT                   /id="VSP_012422"
FT   VARIANT         347
FT                   /note="K -> E (in dbSNP:rs10864628)"
FT                   /evidence="ECO:0000269|PubMed:11891061,
FT                   ECO:0000269|PubMed:15489334"
FT                   /id="VAR_036707"
FT   VARIANT         372
FT                   /note="A -> T (in dbSNP:rs34160967)"
FT                   /id="VAR_036708"
FT   VARIANT         507
FT                   /note="R -> Q (in dbSNP:rs35118458)"
FT                   /id="VAR_036709"
FT   CONFLICT        167
FT                   /note="I -> L (in Ref. 4; AAL91358)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        648
FT                   /note="C -> W (in Ref. 4; AAL91360)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   841 AA;  93074 MW;  E800772B267D0216 CRC64;
     MLLCTARLVG LQLLISCCWA FACHSTESSP DFTLPGDYLL AGLFPLHSGC LQVRHRPEVT
     LCDRSCSFNE HGYHLFQAMR LGVEEINNST ALLPNITLGY QLYDVCSDSA NVYATLRVLS
     LPGQHHIELQ GDLLHYSPTV LAVIGPDSTN RAATTAALLS PFLVPMISYA ASSETLSVKR
     QYPSFLRTIP NDKYQVETMV LLLQKFGWTW ISLVGSSDDY GQLGVQALEN QATGQGICIA
     FKDIMPFSAQ VGDERMQCLM RHLAQAGATV VVVFSSRQLA RVFFESVVLT NLTGKVWVAS
     EAWALSRHIT GVPGIQRIGM VLGVAIQKRA VPGLKAFEEA YARADKKAPR PCHKGSWCSS
     NQLCRECQAF MAHTMPKLKA FSMSSAYNAY RAVYAVAHGL HQLLGCASGA CSRGRVYPWQ
     LLEQIHKVHF LLHKDTVAFN DNRDPLSSYN IIAWDWNGPK WTFTVLGSST WSPVQLNINE
     TKIQWHGKDN QVPKSVCSSD CLEGHQRVVT GFHHCCFECV PCGAGTFLNK SDLYRCQPCG
     KEEWAPEGSQ TCFPRTVVFL ALREHTSWVL LAANTLLLLL LLGTAGLFAW HLDTPVVRSA
     GGRLCFLMLG SLAAGSGSLY GFFGEPTRPA CLLRQALFAL GFTIFLSCLT VRSFQLIIIF
     KFSTKVPTFY HAWVQNHGAG LFVMISSAAQ LLICLTWLVV WTPLPAREYQ RFPHLVMLEC
     TETNSLGFIL AFLYNGLLSI SAFACSYLGK DLPENYNEAK CVTFSLLFNF VSWIAFFTTA
     SVYDGKYLPA ANMMAGLSSL SSGFGGYFLP KCYVILCRPD LNSTEHFQAS IQDYTRRCGS
     T
//