Reviewed, UniProtKB/Swiss-Prot Q7RTX1 (TS1R1_HUMAN)

Names and origin

Protein names

Recommended name:
    Taste receptor type 1 member 1
Alternative name(s):
    G-protein coupled receptor 70

Gene names

Name:	TAS1R1
Synonyms:	GPR70, T1R1, TR1
ORF Names:	GM148

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	841 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate). Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses. Ref.5
Subunit structure	Forms heterodimers with TAS1R3.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 3 family. TAS1R subfamily.
Sequence caution	The sequence BAC05845.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Sensory transduction Taste
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW response to stimulus Inferred from electronic annotation. Source: UniProtKB-KW sensory perception of umami taste Ref.5 Inferred from direct assay. Source: UniProtKB
Cellular component	integral to membrane Ref.5 Inferred by curator. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein heterodimerization activity Ref.5 Non-traceable author statement. Source: UniProtKB taste receptor activity Ref.5 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7RTX1-1)

Also known as: Gm148 form B;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7RTX1-2)

Also known as: Gm148 form C;

The sequence of this isoform differs from the canonical sequence as follows:
     167-420: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q7RTX1-3)

Also known as: Gm148 form A;

The sequence of this isoform differs from the canonical sequence as follows:
     167-420: Missing.
     421-841: LLEQIHKVHF...QDYTRRCGST → LSYAASSETL...LGLLACLPGT

Note: No experimental confirmation available. Ref.5 (AAL91358) sequence differs from that shown due to frameshifts in positions 340 and 548.

Isoform 4 (identifier: Q7RTX1-4)

Also known as: Gm148 form D;

The sequence of this isoform differs from the canonical sequence as follows:
     167-420: Missing.
     532-574: DLYRCQPCGK...HTSWVLLAAN → ATWVRTCQRT...RPASTTAXTC
     575-841: Missing.

Note: Incomplete sequence.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 841	821	Taste receptor type 1 member 1		PRO_0000012954
Regions
	Topological domain	21 – 567	547	Extracellular Potential
	Transmembrane	568 – 588	21	1 Potential
	Topological domain	589 – 603	15	Cytoplasmic Potential
	Transmembrane	604 – 624	21	2 Potential
	Topological domain	625 – 639	15	Extracellular Potential
	Transmembrane	640 – 660	21	3 Potential
	Topological domain	661 – 680	20	Cytoplasmic Potential
	Transmembrane	681 – 701	21	4 Potential
	Topological domain	702 – 725	24	Extracellular Potential
	Transmembrane	726 – 746	21	5 Potential
	Topological domain	747 – 761	15	Cytoplasmic Potential
	Transmembrane	762 – 782	21	6 Potential
	Topological domain	783 – 795	13	Extracellular Potential
	Transmembrane	796 – 816	21	7 Potential
	Topological domain	817 – 841	25	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	87	1	N-linked (GlcNAc...) Potential
	Glycosylation	88	1	N-linked (GlcNAc...) Potential
	Glycosylation	95	1	N-linked (GlcNAc...) Potential
	Glycosylation	291	1	N-linked (GlcNAc...) Potential
	Glycosylation	479	1	N-linked (GlcNAc...) Potential
	Glycosylation	529	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	167 – 420	254	Missing in isoform 2, isoform 3 and isoform 4.		VSP_012418
	Alternative sequence	421 – 841	421	LLEQI…RCGST → LSYAASSETLSVKRQYPSFL RTIPNDKYQVETMVLLLQKF GWTWISLVGSSDDYGQLGVQ ALENQATGQGICIAFKDIMP FSAQVGDERMQCLMRHLAQA GATVVVVFSSRQLARVFFES VVLTNLTGKVWVASEAWALS RHITGVPGIQRIGMVLGVAI QKRAVPGLKAFEEAYARADK KAPRPCHKGSWCSSNQLCRE CQAFMAHTMPKLKAFSMSSA YNAYRAVYAVAHGLHQLLGC ASGACSRGRVYPWQTSTDAS LVGKKSGHLREARPASRALW CFWLCVSTPLGCCWQLTRCC CCCCLGLLACLPGT in isoform 3.		VSP_012420
	Alternative sequence	532 – 574	43	DLYRC…LLAAN → ATWVRTCQRTTTRTNVSPSA CSSTSCPGSPSSPRPASTTA XTC in isoform 4.		VSP_012421
	Alternative sequence	575 – 841	267	Missing in isoform 4.		VSP_012422
	Natural variant	347	1	K → E: dbSNP rs10864628. Ref.3 Ref.4		VAR_036707
	Natural variant	372	1	A → T: dbSNP rs34160967.		VAR_036708
	Natural variant	507	1	R → Q: dbSNP rs35118458.		VAR_036709
Experimental info
	Sequence conflict	167	1	I → L in AAL91358. Ref.4
	Sequence conflict	550	1	Q → R in CAI16076. Ref.2
	Sequence conflict	648	1	C → W in AAL91360. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Gm148 form B) [UniParc]. Last modified December 15, 2003. Version 1. Checksum: E800772B267D0216	FASTA	841	93,074
	10 20 30 40 50 60 MLLCTARLVG LQLLISCCWA FACHSTESSP DFTLPGDYLL AGLFPLHSGC LQVRHRPEVT 70 80 90 100 110 120 LCDRSCSFNE HGYHLFQAMR LGVEEINNST ALLPNITLGY QLYDVCSDSA NVYATLRVLS 130 140 150 160 170 180 LPGQHHIELQ GDLLHYSPTV LAVIGPDSTN RAATTAALLS PFLVPMISYA ASSETLSVKR 190 200 210 220 230 240 QYPSFLRTIP NDKYQVETMV LLLQKFGWTW ISLVGSSDDY GQLGVQALEN QATGQGICIA 250 260 270 280 290 300 FKDIMPFSAQ VGDERMQCLM RHLAQAGATV VVVFSSRQLA RVFFESVVLT NLTGKVWVAS 310 320 330 340 350 360 EAWALSRHIT GVPGIQRIGM VLGVAIQKRA VPGLKAFEEA YARADKKAPR PCHKGSWCSS 370 380 390 400 410 420 NQLCRECQAF MAHTMPKLKA FSMSSAYNAY RAVYAVAHGL HQLLGCASGA CSRGRVYPWQ 430 440 450 460 470 480 LLEQIHKVHF LLHKDTVAFN DNRDPLSSYN IIAWDWNGPK WTFTVLGSST WSPVQLNINE 490 500 510 520 530 540 TKIQWHGKDN QVPKSVCSSD CLEGHQRVVT GFHHCCFECV PCGAGTFLNK SDLYRCQPCG 550 560 570 580 590 600 KEEWAPEGSQ TCFPRTVVFL ALREHTSWVL LAANTLLLLL LLGTAGLFAW HLDTPVVRSA 610 620 630 640 650 660 GGRLCFLMLG SLAAGSGSLY GFFGEPTRPA CLLRQALFAL GFTIFLSCLT VRSFQLIIIF 670 680 690 700 710 720 KFSTKVPTFY HAWVQNHGAG LFVMISSAAQ LLICLTWLVV WTPLPAREYQ RFPHLVMLEC 730 740 750 760 770 780 TETNSLGFIL AFLYNGLLSI SAFACSYLGK DLPENYNEAK CVTFSLLFNF VSWIAFFTTA 790 800 810 820 830 840 SVYDGKYLPA ANMMAGLSSL SSGFGGYFLP KCYVILCRPD LNSTEHFQAS IQDYTRRCGS T
	Isoform 2 (Gm148 form C). Checksum: 236CCE778949C68F	FASTA	587	65,130
	10 20 30 40 50 60 MLLCTARLVG LQLLISCCWA FACHSTESSP DFTLPGDYLL AGLFPLHSGC LQVRHRPEVT 70 80 90 100 110 120 LCDRSCSFNE HGYHLFQAMR LGVEEINNST ALLPNITLGY QLYDVCSDSA NVYATLRVLS 130 140 150 160 170 180 LPGQHHIELQ GDLLHYSPTV LAVIGPDSTN RAATTAALLS PFLVPMLLEQ IHKVHFLLHK 190 200 210 220 230 240 DTVAFNDNRD PLSSYNIIAW DWNGPKWTFT VLGSSTWSPV QLNINETKIQ WHGKDNQVPK 250 260 270 280 290 300 SVCSSDCLEG HQRVVTGFHH CCFECVPCGA GTFLNKSDLY RCQPCGKEEW APEGSQTCFP 310 320 330 340 350 360 RTVVFLALRE HTSWVLLAAN TLLLLLLLGT AGLFAWHLDT PVVRSAGGRL CFLMLGSLAA 370 380 390 400 410 420 GSGSLYGFFG EPTRPACLLR QALFALGFTI FLSCLTVRSF QLIIIFKFST KVPTFYHAWV 430 440 450 460 470 480 QNHGAGLFVM ISSAAQLLIC LTWLVVWTPL PAREYQRFPH LVMLECTETN SLGFILAFLY 490 500 510 520 530 540 NGLLSISAFA CSYLGKDLPE NYNEAKCVTF SLLFNFVSWI AFFTTASVYD GKYLPAANMM 550 560 570 580 AGLSSLSSGF GGYFLPKCYV ILCRPDLNST EHFQASIQDY TRRCGST
	Isoform 3 (Gm148 form A). Checksum: 451E536935DD36D9	FASTA	480	52,522
	10 20 30 40 50 60 MLLCTARLVG LQLLISCCWA FACHSTESSP DFTLPGDYLL AGLFPLHSGC LQVRHRPEVT 70 80 90 100 110 120 LCDRSCSFNE HGYHLFQAMR LGVEEINNST ALLPNITLGY QLYDVCSDSA NVYATLRVLS 130 140 150 160 170 180 LPGQHHIELQ GDLLHYSPTV LAVIGPDSTN RAATTAALLS PFLVPMLSYA ASSETLSVKR 190 200 210 220 230 240 QYPSFLRTIP NDKYQVETMV LLLQKFGWTW ISLVGSSDDY GQLGVQALEN QATGQGICIA 250 260 270 280 290 300 FKDIMPFSAQ VGDERMQCLM RHLAQAGATV VVVFSSRQLA RVFFESVVLT NLTGKVWVAS 310 320 330 340 350 360 EAWALSRHIT GVPGIQRIGM VLGVAIQKRA VPGLKAFEEA YARADKKAPR PCHKGSWCSS 370 380 390 400 410 420 NQLCRECQAF MAHTMPKLKA FSMSSAYNAY RAVYAVAHGL HQLLGCASGA CSRGRVYPWQ 430 440 450 460 470 480 TSTDASLVGK KSGHLREARP ASRALWCFWL CVSTPLGCCW QLTRCCCCCC LGLLACLPGT
	Isoform 4 (Gm148 form D). Checksum: 9A44A1B5A61BA88C	FASTA	320	35,134
	10 20 30 40 50 60 MLLCTARLVG LQLLISCCWA FACHSTESSP DFTLPGDYLL AGLFPLHSGC LQVRHRPEVT 70 80 90 100 110 120 LCDRSCSFNE HGYHLFQAMR LGVEEINNST ALLPNITLGY QLYDVCSDSA NVYATLRVLS 130 140 150 160 170 180 LPGQHHIELQ GDLLHYSPTV LAVIGPDSTN RAATTAALLS PFLVPMLLEQ IHKVHFLLHK 190 200 210 220 230 240 DTVAFNDNRD PLSSYNIIAW DWNGPKWTFT VLGSSTWSPV QLNINETKIQ WHGKDNQVPK 250 260 270 280 290 300 SVCSSDCLEG HQRVVTGFHH CCFECVPCGA GTFLNKSATW VRTCQRTTTR TNVSPSACSS 310 320 TSCPGSPSSP RPASTTAXTC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome-wide discovery and analysis of human seven transmembrane helix receptor genes." Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S., Tsutsumi S., Aburatani H., Asai K., Akiyama Y. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT GLU-347.
[4]	"Identification of six novel genes by experimental validation of GeneMachine predicted genes." Makalowska I., Sood R., Faruque M.U., Hu P., Robbins C.M., Eddings E.M., Mestre J.D., Baxevanis A.D., Carpten J.D. Gene 284:203-213(2002) [PubMed: 11891061] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 65-841 (ISOFORMS 1; 2; 3 AND 4), VARIANT GLU-347.
[5]	"Human receptors for sweet and umami taste." Li X., Staszewski L., Xu H., Durick K., Zoller M., Adler E. Proc. Natl. Acad. Sci. U.S.A. 99:4692-4696(2002) [PubMed: 11917125] [Abstract] Cited for: IDENTIFICATION OF ISOFORM 1, FUNCTION.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

The taste experience - Issue 55 of February 2005

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB065618 Genomic DNA. Translation: BAC05845.1. Sequence problems. AL591866 Genomic DNA. Translation: CAI16073.1. AL591866 Genomic DNA. Translation: CAI16074.1. AL591866 Genomic DNA. Translation: CAI16076.1. BC136515 mRNA. Translation: AAI36516.1. BC136516 mRNA. Translation: AAI36517.1. AF387617 mRNA. Translation: AAL91358.1. Sequence problems. AF387618 mRNA. Translation: AAL91359.1. Different initiation. AF387619 mRNA. Translation: AAL91360.1. Different initiation. AF387620 mRNA. Translation: AAL91361.1. Different initiation. BK000153 mRNA. Translation: DAA00012.1.
IPI	IPI00220275. IPI00291392. IPI00332575. IPI00514989.
RefSeq	NP_619642.2. NP_803883.1. NP_803884.1. NP_803885.1.
UniGene	Hs.124574
3D structure databases
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PRIDE	Q7RTX1.
Genome annotation databases
Ensembl	ENSG00000173662. Homo sapiens. [Contig view]
GeneID	80835.
KEGG	hsa:80835.
Organism-specific databases
GeneCards	GC01P006550.
H-InvDB	HIX0000082.
HGNC	HGNC:14448. TAS1R1.
MIM	606225. gene.
PharmGKB	PA37882.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q7RTX1.
HOVERGEN	Q7RTX1.
OMA	Q7RTX1. KCVTFSL.
Gene expression databases
ArrayExpress	Q7RTX1.
Bgee	Q7RTX1.
GermOnline	ENSG00000173662. Homo sapiens.
Family and domain databases
InterPro	IPR001828. ANF_lig_bd_rcpt. IPR000337. GPCR_3. IPR017978. GPCR_3_C. IPR000068. GPCR_3_Ca_sens_rcpt-rel. IPR017979. GPCR_3_CS. IPR011500. GPCR_3_NCR. [Graphical view]
Pfam	PF00003. 7tm_3. 1 hit. PF01094. ANF_receptor. 1 hit. PF07562. NCD3G. 1 hit. [Graphical view]
PRINTS	PR00592. CASENSINGR. PR00248. GPCRMGR.
PROSITE	PS00979. G_PROTEIN_RECEP_F3_1. False negative. PS00980. G_PROTEIN_RECEP_F3_2. 1 hit. PS00981. G_PROTEIN_RECEP_F3_3. 1 hit. PS50259. G_PROTEIN_RECEP_F3_4. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	71274.
SOURCE	Search...

Entry information

Entry name

TS1R1_HUMAN

Accession

Primary (citable) accession number: Q7RTX1
Secondary accession number(s): B2RMX0 , Q5SY22, Q5SY24, Q8NGZ7, Q8TDJ7, Q8TDJ8, Q8TDJ9, Q8TDK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	December 15, 2003
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)