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Protein

Taste receptor type 1 member 3

Gene

TAS1R3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate). TAS1R2/TAS1R3 recognizes diverse natural and synthetic sweeteners. TAS1R3 is essential for the recognition and response to the disaccharide trehalose (By similarity). Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses.By similarity2 Publications

GO - Molecular functioni

  • protein heterodimerization activity Source: UniProtKB
  • taste receptor activity Source: UniProtKB

GO - Biological processi

  • detection of chemical stimulus involved in sensory perception of sweet taste Source: HGNC
  • G-protein coupled receptor signaling pathway Source: HGNC
  • sensory perception of sweet taste Source: UniProtKB
  • sensory perception of umami taste Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Sensory transduction, Taste

Enzyme and pathway databases

ReactomeiREACT_18319. Class C/3 (Metabotropic glutamate/pheromone receptors).

Names & Taxonomyi

Protein namesi
Recommended name:
Taste receptor type 1 member 3
Alternative name(s):
Sweet taste receptor T1R3
Gene namesi
Name:TAS1R3
Synonyms:T1R3, TR3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:15661. TAS1R3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 570550ExtracellularSequence AnalysisAdd
BLAST
Transmembranei571 – 59121Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini592 – 60312CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei604 – 62421Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini625 – 63915ExtracellularSequence AnalysisAdd
BLAST
Transmembranei640 – 66021Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini661 – 68222CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei683 – 70321Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini704 – 72926ExtracellularSequence AnalysisAdd
BLAST
Transmembranei730 – 75021Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini751 – 76212CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei763 – 78321Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini784 – 7896ExtracellularSequence Analysis
Transmembranei790 – 81021Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini811 – 85242CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB
  • plasma membrane Source: Reactome
  • sweet taste receptor complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi537 – 5371A → G: Retains partial activity toward brazzein; however response to other sweeteners tested is suppressed. 1 Publication
Mutagenesisi537 – 5371A → P: Receptor unresponsive to all sweeteners tested. 1 Publication
Mutagenesisi537 – 5371A → T, S, E or V: Abolished the response to brazzein. 1 Publication
Mutagenesisi540 – 5401F → A or H: Reduces the response to brazzein and monellin. 1 Publication
Mutagenesisi540 – 5401F → L: Reduces the response to monellin. 1 Publication
Mutagenesisi540 – 5401F → Y or P: Reduces the response to brazzein; P-540 also enhances responses to the small molecule sweeteners. 1 Publication

Organism-specific databases

PharmGKBiPA38012.

Polymorphism and mutation databases

BioMutaiTAS1R3.
DMDMi62299063.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 852832Taste receptor type 1 member 3PRO_0000012961Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi85 – 851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi130 – 1301N-linked (GlcNAc...)Sequence Analysis
Glycosylationi264 – 2641N-linked (GlcNAc...)Sequence Analysis
Glycosylationi285 – 2851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi380 – 3801N-linked (GlcNAc...)Sequence Analysis
Glycosylationi411 – 4111N-linked (GlcNAc...)Sequence Analysis
Glycosylationi432 – 4321N-linked (GlcNAc...)Sequence Analysis
Glycosylationi475 – 4751N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ7RTX0.
PRIDEiQ7RTX0.

Expressioni

Gene expression databases

BgeeiQ7RTX0.
CleanExiHS_TAS1R3.
GenevestigatoriQ7RTX0.

Organism-specific databases

HPAiHPA053439.

Interactioni

Subunit structurei

Forms homodimers or heterodimers with TAS1R1 and TAS1R2.

Protein-protein interaction databases

BioGridi123757. 2 interactions.
STRINGi9606.ENSP00000344411.

Structurei

3D structure databases

ProteinModelPortaliQ7RTX0.
SMRiQ7RTX0. Positions 25-555, 560-824.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni536 – 54510Required for brazzein responsiveness

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG303330.
GeneTreeiENSGT00760000118884.
HOGENOMiHOG000231293.
HOVERGENiHBG098572.
InParanoidiQ7RTX0.
KOiK04626.
OMAiQLLENMY.
OrthoDBiEOG7K0ZBJ.
PhylomeDBiQ7RTX0.
TreeFamiTF331269.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7RTX0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGPAVLGLS LWALLHPGTG APLCLSQQLR MKGDYVLGGL FPLGEAEEAG
60 70 80 90 100
LRSRTRPSSP VCTRFSSNGL LWALAMKMAV EEINNKSDLL PGLRLGYDLF
110 120 130 140 150
DTCSEPVVAM KPSLMFLAKA GSRDIAAYCN YTQYQPRVLA VIGPHSSELA
160 170 180 190 200
MVTGKFFSFF LMPQVSYGAS MELLSARETF PSFFRTVPSD RVQLTAAAEL
210 220 230 240 250
LQEFGWNWVA ALGSDDEYGR QGLSIFSALA AARGICIAHE GLVPLPRADD
260 270 280 290 300
SRLGKVQDVL HQVNQSSVQV VLLFASVHAA HALFNYSISS RLSPKVWVAS
310 320 330 340 350
EAWLTSDLVM GLPGMAQMGT VLGFLQRGAQ LHEFPQYVKT HLALATDPAF
360 370 380 390 400
CSALGEREQG LEEDVVGQRC PQCDCITLQN VSAGLNHHQT FSVYAAVYSV
410 420 430 440 450
AQALHNTLQC NASGCPAQDP VKPWQLLENM YNLTFHVGGL PLRFDSSGNV
460 470 480 490 500
DMEYDLKLWV WQGSVPRLHD VGRFNGSLRT ERLKIRWHTS DNQKPVSRCS
510 520 530 540 550
RQCQEGQVRR VKGFHSCCYD CVDCEAGSYR QNPDDIACTF CGQDEWSPER
560 570 580 590 600
STRCFRRRSR FLAWGEPAVL LLLLLLSLAL GLVLAALGLF VHHRDSPLVQ
610 620 630 640 650
ASGGPLACFG LVCLGLVCLS VLLFPGQPSP ARCLAQQPLS HLPLTGCLST
660 670 680 690 700
LFLQAAEIFV ESELPLSWAD RLSGCLRGPW AWLVVLLAML VEVALCTWYL
710 720 730 740 750
VAFPPEVVTD WHMLPTEALV HCRTRSWVSF GLAHATNATL AFLCFLGTFL
760 770 780 790 800
VRSQPGCYNR ARGLTFAMLA YFITWVSFVP LLANVQVVLR PAVQMGALLL
810 820 830 840 850
CVLGILAAFH LPRCYLLMRQ PGLNTPEFFL GGGPGDAQGQ NDGNTGNQGK

HE
Length:852
Mass (Da):93,386
Last modified:March 29, 2005 - v2
Checksum:i9690DEC23E75EB3E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti852 – 8521E → EAPILHPARHHSSGRPGGGT QAYAQGRARRGRGGTQLPTL PFAQWVDSIMTVTSTRDRAQ DLTPARRQAHTGSPVRVPGS APSRA in BAC05873 (Ref. 1) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti757 – 7571C → R.
Corresponds to variant rs307377 [ dbSNP | Ensembl ].
VAR_020788

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB065647 Genomic DNA. Translation: BAC05873.1.
AL139287 Genomic DNA. Translation: CAI23184.1.
BK000152 mRNA. Translation: DAA00013.1.
CCDSiCCDS30556.1.
RefSeqiNP_689414.1. NM_152228.1.
UniGeneiHs.74375.

Genome annotation databases

EnsembliENST00000339381; ENSP00000344411; ENSG00000169962.
GeneIDi83756.
KEGGihsa:83756.
UCSCiuc010nyk.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Protein Spotlight

The taste experience - Issue 55 of February 2005

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB065647 Genomic DNA. Translation: BAC05873.1.
AL139287 Genomic DNA. Translation: CAI23184.1.
BK000152 mRNA. Translation: DAA00013.1.
CCDSiCCDS30556.1.
RefSeqiNP_689414.1. NM_152228.1.
UniGeneiHs.74375.

3D structure databases

ProteinModelPortaliQ7RTX0.
SMRiQ7RTX0. Positions 25-555, 560-824.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123757. 2 interactions.
STRINGi9606.ENSP00000344411.

Protein family/group databases

GPCRDBiSearch...

Polymorphism and mutation databases

BioMutaiTAS1R3.
DMDMi62299063.

Proteomic databases

PaxDbiQ7RTX0.
PRIDEiQ7RTX0.

Protocols and materials databases

DNASUi83756.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000339381; ENSP00000344411; ENSG00000169962.
GeneIDi83756.
KEGGihsa:83756.
UCSCiuc010nyk.2. human.

Organism-specific databases

CTDi83756.
GeneCardsiGC01P001266.
HGNCiHGNC:15661. TAS1R3.
HPAiHPA053439.
MIMi605865. gene.
neXtProtiNX_Q7RTX0.
PharmGKBiPA38012.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG303330.
GeneTreeiENSGT00760000118884.
HOGENOMiHOG000231293.
HOVERGENiHBG098572.
InParanoidiQ7RTX0.
KOiK04626.
OMAiQLLENMY.
OrthoDBiEOG7K0ZBJ.
PhylomeDBiQ7RTX0.
TreeFamiTF331269.

Enzyme and pathway databases

ReactomeiREACT_18319. Class C/3 (Metabotropic glutamate/pheromone receptors).

Miscellaneous databases

GeneWikiiTAS1R3.
GenomeRNAii83756.
NextBioi72785.
PROiQ7RTX0.
SOURCEiSearch...

Gene expression databases

BgeeiQ7RTX0.
CleanExiHS_TAS1R3.
GenevestigatoriQ7RTX0.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome-wide discovery and analysis of human seven transmembrane helix receptor genes."
    Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S., Tsutsumi S., Aburatani H., Asai K., Akiyama Y.
    Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: IDENTIFICATION, FUNCTION.
  4. "Taste receptor T1R3 is an essential molecule for the cellular recognition of the disaccharide trehalose."
    Ariyasu T., Matsumoto S., Kyono F., Hanaya T., Arai S., Ikeda M., Kurimoto M.
    In Vitro Cell. Dev. Biol. Anim. 39:80-88(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "The cysteine-rich region of T1R3 determines responses to intensely sweet proteins."
    Jiang P., Ji Q., Liu Z., Snyder L.A., Benard L.M.J., Margolskee R.F., Max M.
    J. Biol. Chem. 279:45068-45075(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: REGION IMPLICATED IN SWEET RECEPTOR FUNCTION, MUTAGENESIS OF ALA-537 AND PHE-540.

Entry informationi

Entry nameiTS1R3_HUMAN
AccessioniPrimary (citable) accession number: Q7RTX0
Secondary accession number(s): Q5TA49, Q8NGW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: March 29, 2005
Last modified: May 27, 2015
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.