Reviewed,
UniProtKB/Swiss-Prot Q7RTX0 (TS1R3_HUMAN)
Last modified
July 22, 2008.
Version 52.
History...
Clusters with 100%,
90%,
50% identity |
Documents (8) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Taste receptor type 1 member 3 Alternative name(s): Sweet taste receptor T1R3 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 852 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate). TAS1R2/TAS1R3 recognizes diverse natural and synthetic sweeteners. TAS1R3 is essential for the recognition and response to the disaccharide trehalose By similarity. Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses. |
| Subunit structure | Forms homodimers or heterodimers with TAS1R1 and TAS1R2. |
| Subcellular location | |
| Sequence similarities | Belongs to the G-protein coupled receptor 3 family. TAS1R subfamily. |
Ontologies
Keywords | |
|---|---|
| Biological process | Sensory transduction Taste |
| Cellular component | Cell membrane Membrane |
| Coding sequence diversity | Polymorphism |
| Domain | Signal Transmembrane |
| Molecular function | G-protein coupled receptor Receptor Transducer |
| PTM | Glycoprotein |
Gene Ontology (GO) | |
| Biological process | G-protein coupled receptor protein signaling pathway Inferred by curator. Source: HGNC detection of chemical stimulus involved in sensory perception of sweet tasteInferred from direct assay. Source: HGNC sensory perception of umami taste Ref.3Inferred from direct assay. Source: UniProtKB |
| Cellular component | integral to membrane Ref.3 Inferred by curator. Source: UniProtKB |
| Molecular function | protein heterodimerization activity Ref.3 Non-traceable author statement. Source: UniProtKB taste receptor activity Ref.3Inferred from direct assay. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | ||||
Molecule processing | ||||||||
|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 20 | 20 | Potential | |||||
| Chain | 21 – 852 | 832 | Taste receptor type 1 member 3 | |||||
Regions | ||||||||
| Topological domain | 21 – 570 | 550 | Extracellular Potential | |||||
| Transmembrane | 571 – 591 | 21 | 1 Potential | |||||
| Topological domain | 592 – 603 | 12 | Cytoplasmic Potential | |||||
| Transmembrane | 604 – 624 | 21 | 2 Potential | |||||
| Topological domain | 625 – 639 | 15 | Extracellular Potential | |||||
| Transmembrane | 640 – 660 | 21 | 3 Potential | |||||
| Topological domain | 661 – 682 | 22 | Cytoplasmic Potential | |||||
| Transmembrane | 683 – 703 | 21 | 4 Potential | |||||
| Topological domain | 704 – 729 | 26 | Extracellular Potential | |||||
| Transmembrane | 730 – 750 | 21 | 5 Potential | |||||
| Topological domain | 751 – 762 | 12 | Cytoplasmic Potential | |||||
| Transmembrane | 763 – 783 | 21 | 6 Potential | |||||
| Topological domain | 784 – 789 | 6 | Extracellular Potential | |||||
| Transmembrane | 790 – 810 | 21 | 7 Potential | |||||
| Topological domain | 811 – 852 | 42 | Cytoplasmic Potential | |||||
| Region | 536 – 545 | 10 | Required for brazzein responsiveness | |||||
Amino acid modifications | ||||||||
| Glycosylation | 85 | 1 | N-linked (GlcNAc...) Potential | |||||
| Glycosylation | 130 | 1 | N-linked (GlcNAc...) Potential | |||||
| Glycosylation | 264 | 1 | N-linked (GlcNAc...) Potential | |||||
| Glycosylation | 285 | 1 | N-linked (GlcNAc...) Potential | |||||
| Glycosylation | 380 | 1 | N-linked (GlcNAc...) Potential | |||||
| Glycosylation | 411 | 1 | N-linked (GlcNAc...) Potential | |||||
| Glycosylation | 432 | 1 | N-linked (GlcNAc...) Potential | |||||
| Glycosylation | 475 | 1 | N-linked (GlcNAc...) Potential | |||||
Natural variations | ||||||||
| Natural variant | 757 | 1 | C → R: dbSNP rs307377. | |||||
Experimental info | ||||||||
| Mutagenesis | 537 | 1 | A → G: Retains partial activity toward brazzein; however response to other sweeteners tested is suppressed | |||||
| Mutagenesis | 537 | 1 | A → P: Receptor unresponsive to all sweeteners tested | |||||
| Mutagenesis | 537 | 1 | A → T, S, E or V: Abolished the response to brazzein | |||||
| Mutagenesis | 540 | 1 | F → A or H: Reduces the response to brazzein and monellin | |||||
| Mutagenesis | 540 | 1 | F → L: Reduces the response to monellin | |||||
| Mutagenesis | 540 | 1 | F → Y or P: Reduces the response to brazzein; P-540 also enhances responses to the small molecule sweeteners | |||||
| Sequence conflict | 852 | 1 | E → EAPILHPARHHSSGRPGGGT QAYAQGRARRGRGGTQLPTL PFAQWVDSIMTVTSTRDRAQ DLTPARRQAHTGSPVRVPGS APSRA in BAC05873. Ref.1 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Genome-wide discovery and analysis of human seven transmembrane helix receptor genes." Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S., Tsutsumi S., Aburatani H., Asai K., Akiyama Y. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. |
| [2] | "The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. Bentley D.R.Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | "Human receptors for sweet and umami taste." Li X., Staszewski L., Xu H., Durick K., Zoller M., Adler E. Proc. Natl. Acad. Sci. U.S.A. 99:4692-4696(2002) [PubMed: 11917125] [Abstract] Cited for: IDENTIFICATION, FUNCTION. |
| [4] | "Taste receptor T1R3 is an essential molecule for the cellular recognition of the disaccharide trehalose." Ariyasu T., Matsumoto S., Kyono F., Hanaya T., Arai S., Ikeda M., Kurimoto M. In Vitro Cell. Dev. Biol. Anim. 39:80-88(2003) [PubMed: 12892531] [Abstract] Cited for: FUNCTION. |
| [5] | "The cysteine-rich region of T1R3 determines responses to intensely sweet proteins." Jiang P., Ji Q., Liu Z., Snyder L.A., Benard L.M.J., Margolskee R.F., Max M. J. Biol. Chem. 279:45068-45075(2004) [PubMed: 15299024] [Abstract] Cited for: REGION IMPLICATED IN SWEET RECEPTOR FUNCTION, MUTAGENESIS OF ALA-537 AND PHE-540. |
Cross-references
Sequence databases | |
|---|---|
| AB065647 Genomic DNA. Translation: BAC05873.1. AL139287 Genomic DNA. Translation: CAI23184.1. BK000152 mRNA. Translation: DAA00013.1. | |
| RefSeq | NP_689414.1. |
| UniGene | Hs.74375 |
3D structure databases | |
| ModBase | Search... |
Protein family/group databases | |
| GPCRDB | Search... |
Genome annotation databases | |
| Ensembl | ENSG00000169962. Homo sapiens. [Contig view] |
| GeneID | 83756. |
| KEGG | hsa:83756. |
Organism-specific databases | |
| HGNC | HGNC:15661. TAS1R3. |
| MIM | 605865. gene. |
| PharmGKB | PA38012. |
| GenAtlas | Search... |
| GeneCards | Search... |
| GeneLynx | Search... |
Phylogenomic databases | |
| HOGENOM | Q7RTX0. |
| HOVERGEN | Q7RTX0. |
Gene expression databases | |
| ArrayExpress | Q7RTX0. |
| CleanEx | HS_TAS1R3. |
Family and domain databases | |
| InterPro | IPR001828. ANF_lig_bd_rcpt. IPR000337. GPCR_3. IPR000068. GPCR_3_Ca_sens_rcpt-rel. IPR011500. GPCR_3_NCR. [Graphical view] |
| Pfam | PF00003. 7tm_3. 1 hit. PF01094. ANF_receptor. 1 hit. PF07562. NCD3G. 1 hit. [Graphical view] |
| PRINTS | PR00592. CASENSINGR. PR00248. GPCRMGR. |
| PROSITE | PS00979. G_PROTEIN_RECEP_F3_1. False negative. PS00980. G_PROTEIN_RECEP_F3_2. 1 hit. PS00981. G_PROTEIN_RECEP_F3_3. False negative. PS50259. G_PROTEIN_RECEP_F3_4. 1 hit. [Graphical view] |
| ProDom | Q7RTX0. [Graphical view] [Entries sharing at least one domain] |
| BLOCKS | Search... |
Other Resources | |
| DrugBank | DB00168. Aspartame. |
| SOURCE | Search... |
| ProtoNet | Search... |
Entry information
| Entry name | TS1R3_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q7RTX0 Secondary accession number(s): Q5TA49, Q8NGW9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| 7-transmembrane G-linked receptors List of 7-transmembrane G-linked receptor entries |
| Human chromosome 1 Human chromosome 1: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| Protein Spotlight Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries |
| UniProtKB secondary accession numbers Index of UniProtKB secondary accession numbers |
| SIMILARITY comments Index of protein domains and families |

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