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Q7RTX0 (TS1R3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Taste receptor type 1 member 3
Alternative name(s):
Sweet taste receptor T1R3
Gene names
Name:TAS1R3
Synonyms:T1R3, TR3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length852 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate). TAS1R2/TAS1R3 recognizes diverse natural and synthetic sweeteners. TAS1R3 is essential for the recognition and response to the disaccharide trehalose By similarity. Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses. Ref.3 Ref.4 Ref.5

Subunit structure

Forms homodimers or heterodimers with TAS1R1 and TAS1R2.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the G-protein coupled receptor 3 family. TAS1R subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 852832Taste receptor type 1 member 3
PRO_0000012961

Regions

Topological domain21 – 570550Extracellular Potential
Transmembrane571 – 59121Helical; Name=1; Potential
Topological domain592 – 60312Cytoplasmic Potential
Transmembrane604 – 62421Helical; Name=2; Potential
Topological domain625 – 63915Extracellular Potential
Transmembrane640 – 66021Helical; Name=3; Potential
Topological domain661 – 68222Cytoplasmic Potential
Transmembrane683 – 70321Helical; Name=4; Potential
Topological domain704 – 72926Extracellular Potential
Transmembrane730 – 75021Helical; Name=5; Potential
Topological domain751 – 76212Cytoplasmic Potential
Transmembrane763 – 78321Helical; Name=6; Potential
Topological domain784 – 7896Extracellular Potential
Transmembrane790 – 81021Helical; Name=7; Potential
Topological domain811 – 85242Cytoplasmic Potential
Region536 – 54510Required for brazzein responsiveness

Amino acid modifications

Glycosylation851N-linked (GlcNAc...) Potential
Glycosylation1301N-linked (GlcNAc...) Potential
Glycosylation2641N-linked (GlcNAc...) Potential
Glycosylation2851N-linked (GlcNAc...) Potential
Glycosylation3801N-linked (GlcNAc...) Potential
Glycosylation4111N-linked (GlcNAc...) Potential
Glycosylation4321N-linked (GlcNAc...) Potential
Glycosylation4751N-linked (GlcNAc...) Potential

Natural variations

Natural variant7571C → R.
Corresponds to variant rs307377 [ dbSNP | Ensembl ].
VAR_020788

Experimental info

Mutagenesis5371A → G: Retains partial activity toward brazzein; however response to other sweeteners tested is suppressed. Ref.5
Mutagenesis5371A → P: Receptor unresponsive to all sweeteners tested. Ref.5
Mutagenesis5371A → T, S, E or V: Abolished the response to brazzein. Ref.5
Mutagenesis5401F → A or H: Reduces the response to brazzein and monellin. Ref.5
Mutagenesis5401F → L: Reduces the response to monellin. Ref.5
Mutagenesis5401F → Y or P: Reduces the response to brazzein; P-540 also enhances responses to the small molecule sweeteners. Ref.5
Sequence conflict8521E → EAPILHPARHHSSGRPGGGT QAYAQGRARRGRGGTQLPTL PFAQWVDSIMTVTSTRDRAQ DLTPARRQAHTGSPVRVPGS APSRA in BAC05873. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q7RTX0 [UniParc].

Last modified March 29, 2005. Version 2.
Checksum: 9690DEC23E75EB3E

FASTA85293,386
        10         20         30         40         50         60 
MLGPAVLGLS LWALLHPGTG APLCLSQQLR MKGDYVLGGL FPLGEAEEAG LRSRTRPSSP 

        70         80         90        100        110        120 
VCTRFSSNGL LWALAMKMAV EEINNKSDLL PGLRLGYDLF DTCSEPVVAM KPSLMFLAKA 

       130        140        150        160        170        180 
GSRDIAAYCN YTQYQPRVLA VIGPHSSELA MVTGKFFSFF LMPQVSYGAS MELLSARETF 

       190        200        210        220        230        240 
PSFFRTVPSD RVQLTAAAEL LQEFGWNWVA ALGSDDEYGR QGLSIFSALA AARGICIAHE 

       250        260        270        280        290        300 
GLVPLPRADD SRLGKVQDVL HQVNQSSVQV VLLFASVHAA HALFNYSISS RLSPKVWVAS 

       310        320        330        340        350        360 
EAWLTSDLVM GLPGMAQMGT VLGFLQRGAQ LHEFPQYVKT HLALATDPAF CSALGEREQG 

       370        380        390        400        410        420 
LEEDVVGQRC PQCDCITLQN VSAGLNHHQT FSVYAAVYSV AQALHNTLQC NASGCPAQDP 

       430        440        450        460        470        480 
VKPWQLLENM YNLTFHVGGL PLRFDSSGNV DMEYDLKLWV WQGSVPRLHD VGRFNGSLRT 

       490        500        510        520        530        540 
ERLKIRWHTS DNQKPVSRCS RQCQEGQVRR VKGFHSCCYD CVDCEAGSYR QNPDDIACTF 

       550        560        570        580        590        600 
CGQDEWSPER STRCFRRRSR FLAWGEPAVL LLLLLLSLAL GLVLAALGLF VHHRDSPLVQ 

       610        620        630        640        650        660 
ASGGPLACFG LVCLGLVCLS VLLFPGQPSP ARCLAQQPLS HLPLTGCLST LFLQAAEIFV 

       670        680        690        700        710        720 
ESELPLSWAD RLSGCLRGPW AWLVVLLAML VEVALCTWYL VAFPPEVVTD WHMLPTEALV 

       730        740        750        760        770        780 
HCRTRSWVSF GLAHATNATL AFLCFLGTFL VRSQPGCYNR ARGLTFAMLA YFITWVSFVP 

       790        800        810        820        830        840 
LLANVQVVLR PAVQMGALLL CVLGILAAFH LPRCYLLMRQ PGLNTPEFFL GGGPGDAQGQ 

       850 
NDGNTGNQGK HE 

« Hide

References

« Hide 'large scale' references
[1]"Genome-wide discovery and analysis of human seven transmembrane helix receptor genes."
Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S., Tsutsumi S., Aburatani H., Asai K., Akiyama Y.
Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Human receptors for sweet and umami taste."
Li X., Staszewski L., Xu H., Durick K., Zoller M., Adler E.
Proc. Natl. Acad. Sci. U.S.A. 99:4692-4696(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, FUNCTION.
[4]"Taste receptor T1R3 is an essential molecule for the cellular recognition of the disaccharide trehalose."
Ariyasu T., Matsumoto S., Kyono F., Hanaya T., Arai S., Ikeda M., Kurimoto M.
In Vitro Cell. Dev. Biol. Anim. 39:80-88(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"The cysteine-rich region of T1R3 determines responses to intensely sweet proteins."
Jiang P., Ji Q., Liu Z., Snyder L.A., Benard L.M.J., Margolskee R.F., Max M.
J. Biol. Chem. 279:45068-45075(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: REGION IMPLICATED IN SWEET RECEPTOR FUNCTION, MUTAGENESIS OF ALA-537 AND PHE-540.
+Additional computationally mapped references.

Web resources

Protein Spotlight

The taste experience - Issue 55 of February 2005

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB065647 Genomic DNA. Translation: BAC05873.1.
AL139287 Genomic DNA. Translation: CAI23184.1.
BK000152 mRNA. Translation: DAA00013.1.
RefSeqNP_689414.1. NM_152228.1.
UniGeneHs.74375.

3D structure databases

ProteinModelPortalQ7RTX0.
SMRQ7RTX0. Positions 25-555.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123757. 2 interactions.
STRING9606.ENSP00000344411.

Chemistry

DrugBankDB00168. Aspartame.

Protein family/group databases

GPCRDBSearch...

Polymorphism databases

DMDM62299063.

Proteomic databases

PaxDbQ7RTX0.
PRIDEQ7RTX0.

Protocols and materials databases

DNASU83756.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000339381; ENSP00000344411; ENSG00000169962.
GeneID83756.
KEGGhsa:83756.
UCSCuc010nyk.2. human.

Organism-specific databases

CTD83756.
GeneCardsGC01P001266.
HGNCHGNC:15661. TAS1R3.
HPAHPA053439.
MIM605865. gene.
neXtProtNX_Q7RTX0.
PharmGKBPA38012.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG303330.
HOGENOMHOG000231293.
HOVERGENHBG098572.
InParanoidQ7RTX0.
KOK04626.
OMAQLLENMY.
OrthoDBEOG7K0ZBJ.
PhylomeDBQ7RTX0.
TreeFamTF331269.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

BgeeQ7RTX0.
CleanExHS_TAS1R3.
GenevestigatorQ7RTX0.

Family and domain databases

InterProIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSPR00248. GPCRMGR.
SUPFAMSSF53822. SSF53822. 1 hit.
PROSITEPS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiTAS1R3.
GenomeRNAi83756.
NextBio72785.
PROQ7RTX0.
SOURCESearch...

Entry information

Entry nameTS1R3_HUMAN
AccessionPrimary (citable) accession number: Q7RTX0
Secondary accession number(s): Q5TA49, Q8NGW9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: March 29, 2005
Last modified: April 16, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries