Reviewed, UniProtKB/Swiss-Prot Q7RTX0 (TS1R3_HUMAN)

Names and origin

Protein names

Recommended name:
    Taste receptor type 1 member 3
Alternative name(s):
    Sweet taste receptor T1R3

Gene names

Name:	TAS1R3
Synonyms:	T1R3, TR3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	852 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate). TAS1R2/TAS1R3 recognizes diverse natural and synthetic sweeteners. TAS1R3 is essential for the recognition and response to the disaccharide trehalose By similarity. Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses.
Subunit structure	Forms homodimers or heterodimers with TAS1R1 and TAS1R2.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 3 family. TAS1R subfamily.

Ontologies

Keywords
Biological process	Sensory transduction Taste
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred by curator. Source: HGNC detection of chemical stimulus involved in sensory perception of sweet taste Inferred from direct assay. Source: HGNC sensory perception of umami taste Ref.3 Inferred from direct assay. Source: UniProtKB
Cellular component	integral to membrane Ref.3 Inferred by curator. Source: UniProtKB
Molecular function	protein heterodimerization activity Ref.3 Non-traceable author statement. Source: UniProtKB taste receptor activity Ref.3 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 852	832	Taste receptor type 1 member 3
Regions
	Topological domain	21 – 570	550	Extracellular Potential
	Transmembrane	571 – 591	21	1 Potential
	Topological domain	592 – 603	12	Cytoplasmic Potential
	Transmembrane	604 – 624	21	2 Potential
	Topological domain	625 – 639	15	Extracellular Potential
	Transmembrane	640 – 660	21	3 Potential
	Topological domain	661 – 682	22	Cytoplasmic Potential
	Transmembrane	683 – 703	21	4 Potential
	Topological domain	704 – 729	26	Extracellular Potential
	Transmembrane	730 – 750	21	5 Potential
	Topological domain	751 – 762	12	Cytoplasmic Potential
	Transmembrane	763 – 783	21	6 Potential
	Topological domain	784 – 789	6	Extracellular Potential
	Transmembrane	790 – 810	21	7 Potential
	Topological domain	811 – 852	42	Cytoplasmic Potential
	Region	536 – 545	10	Required for brazzein responsiveness
Amino acid modifications
	Glycosylation	85	1	N-linked (GlcNAc...) Potential
	Glycosylation	130	1	N-linked (GlcNAc...) Potential
	Glycosylation	264	1	N-linked (GlcNAc...) Potential
	Glycosylation	285	1	N-linked (GlcNAc...) Potential
	Glycosylation	380	1	N-linked (GlcNAc...) Potential
	Glycosylation	411	1	N-linked (GlcNAc...) Potential
	Glycosylation	432	1	N-linked (GlcNAc...) Potential
	Glycosylation	475	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	757	1	C → R: dbSNP rs307377.
Experimental info
	Mutagenesis	537	1	A → G: Retains partial activity toward brazzein; however response to other sweeteners tested is suppressed
	Mutagenesis	537	1	A → P: Receptor unresponsive to all sweeteners tested
	Mutagenesis	537	1	A → T, S, E or V: Abolished the response to brazzein
	Mutagenesis	540	1	F → A or H: Reduces the response to brazzein and monellin
	Mutagenesis	540	1	F → L: Reduces the response to monellin
	Mutagenesis	540	1	F → Y or P: Reduces the response to brazzein; P-540 also enhances responses to the small molecule sweeteners
	Sequence conflict	852	1	E → EAPILHPARHHSSGRPGGGT QAYAQGRARRGRGGTQLPTL PFAQWVDSIMTVTSTRDRAQ DLTPARRQAHTGSPVRVPGS APSRA in BAC05873. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q7RTX0-1 [UniParc]. Last modified March 29, 2005. Version 2. Checksum: 9690DEC23E75EB3E	FASTA	852	93,386
	10 20 30 40 50 60 MLGPAVLGLS LWALLHPGTG APLCLSQQLR MKGDYVLGGL FPLGEAEEAG LRSRTRPSSP 70 80 90 100 110 120 VCTRFSSNGL LWALAMKMAV EEINNKSDLL PGLRLGYDLF DTCSEPVVAM KPSLMFLAKA 130 140 150 160 170 180 GSRDIAAYCN YTQYQPRVLA VIGPHSSELA MVTGKFFSFF LMPQVSYGAS MELLSARETF 190 200 210 220 230 240 PSFFRTVPSD RVQLTAAAEL LQEFGWNWVA ALGSDDEYGR QGLSIFSALA AARGICIAHE 250 260 270 280 290 300 GLVPLPRADD SRLGKVQDVL HQVNQSSVQV VLLFASVHAA HALFNYSISS RLSPKVWVAS 310 320 330 340 350 360 EAWLTSDLVM GLPGMAQMGT VLGFLQRGAQ LHEFPQYVKT HLALATDPAF CSALGEREQG 370 380 390 400 410 420 LEEDVVGQRC PQCDCITLQN VSAGLNHHQT FSVYAAVYSV AQALHNTLQC NASGCPAQDP 430 440 450 460 470 480 VKPWQLLENM YNLTFHVGGL PLRFDSSGNV DMEYDLKLWV WQGSVPRLHD VGRFNGSLRT 490 500 510 520 530 540 ERLKIRWHTS DNQKPVSRCS RQCQEGQVRR VKGFHSCCYD CVDCEAGSYR QNPDDIACTF 550 560 570 580 590 600 CGQDEWSPER STRCFRRRSR FLAWGEPAVL LLLLLLSLAL GLVLAALGLF VHHRDSPLVQ 610 620 630 640 650 660 ASGGPLACFG LVCLGLVCLS VLLFPGQPSP ARCLAQQPLS HLPLTGCLST LFLQAAEIFV 670 680 690 700 710 720 ESELPLSWAD RLSGCLRGPW AWLVVLLAML VEVALCTWYL VAFPPEVVTD WHMLPTEALV 730 740 750 760 770 780 HCRTRSWVSF GLAHATNATL AFLCFLGTFL VRSQPGCYNR ARGLTFAMLA YFITWVSFVP 790 800 810 820 830 840 LLANVQVVLR PAVQMGALLL CVLGILAAFH LPRCYLLMRQ PGLNTPEFFL GGGPGDAQGQ 850 NDGNTGNQGK HE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome-wide discovery and analysis of human seven transmembrane helix receptor genes." Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S., Tsutsumi S., Aburatani H., Asai K., Akiyama Y. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Human receptors for sweet and umami taste." Li X., Staszewski L., Xu H., Durick K., Zoller M., Adler E. Proc. Natl. Acad. Sci. U.S.A. 99:4692-4696(2002) [PubMed: 11917125] [Abstract] Cited for: IDENTIFICATION, FUNCTION.
[4]	"Taste receptor T1R3 is an essential molecule for the cellular recognition of the disaccharide trehalose." Ariyasu T., Matsumoto S., Kyono F., Hanaya T., Arai S., Ikeda M., Kurimoto M. In Vitro Cell. Dev. Biol. Anim. 39:80-88(2003) [PubMed: 12892531] [Abstract] Cited for: FUNCTION.
[5]	"The cysteine-rich region of T1R3 determines responses to intensely sweet proteins." Jiang P., Ji Q., Liu Z., Snyder L.A., Benard L.M.J., Margolskee R.F., Max M. J. Biol. Chem. 279:45068-45075(2004) [PubMed: 15299024] [Abstract] Cited for: REGION IMPLICATED IN SWEET RECEPTOR FUNCTION, MUTAGENESIS OF ALA-537 AND PHE-540.

Web resources

Protein Spotlight

The taste experience - Issue 55 of February 2005

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB065647 Genomic DNA. Translation: BAC05873.1. AL139287 Genomic DNA. Translation: CAI23184.1. BK000152 mRNA. Translation: DAA00013.1.
RefSeq	NP_689414.1.
UniGene	Hs.74375
3D structure databases
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSG00000169962. Homo sapiens. [Contig view]
GeneID	83756.
KEGG	hsa:83756.
Organism-specific databases
HGNC	HGNC:15661. TAS1R3.
MIM	605865. gene.
PharmGKB	PA38012.
GenAtlas	Search...
GeneCards	Search...
GeneLynx	Search...
Phylogenomic databases
HOGENOM	Q7RTX0.
HOVERGEN	Q7RTX0.
Gene expression databases
ArrayExpress	Q7RTX0.
CleanEx	HS_TAS1R3.
Family and domain databases
InterPro	IPR001828. ANF_lig_bd_rcpt. IPR000337. GPCR_3. IPR000068. GPCR_3_Ca_sens_rcpt-rel. IPR011500. GPCR_3_NCR. [Graphical view]
Pfam	PF00003. 7tm_3. 1 hit. PF01094. ANF_receptor. 1 hit. PF07562. NCD3G. 1 hit. [Graphical view]
PRINTS	PR00592. CASENSINGR. PR00248. GPCRMGR.
PROSITE	PS00979. G_PROTEIN_RECEP_F3_1. False negative. PS00980. G_PROTEIN_RECEP_F3_2. 1 hit. PS00981. G_PROTEIN_RECEP_F3_3. False negative. PS50259. G_PROTEIN_RECEP_F3_4. 1 hit. [Graphical view]
ProDom	Q7RTX0. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
DrugBank	DB00168. Aspartame.
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

TS1R3_HUMAN

Accession

Primary (citable) accession number: Q7RTX0
Secondary accession number(s): Q5TA49, Q8NGW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	March 29, 2005
Last modified:	July 22, 2008
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)