Reviewed, UniProtKB/Swiss-Prot Q7RTN6 (STRAA_HUMAN)

Names and origin

Protein names

Recommended name:
    STE20-related kinase adapter protein alpha
      Short name=STRAD alpha
Alternative name(s):
    STE20-related adapter protein
    Serologically defined breast cancer antigen NY-BR-96

Gene names

Name:	STRADA
Synonyms:	LYK5, STRAD

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Pseudokinase which, in complex with CAB39, binds to and activates STK11. Relocates STK11 from the nucleus to the cytoplasm. Plays an essential role in STK11-mediated G1 cell cycle arrest. Ref.4 Ref.5
Subcellular location	Nucleus. Cytoplasm. Ref.4 Ref.5
Domain	The protein kinase domain is predicted to be catalytically inactive.
Involvement in disease	Deletions involving STRADA are the cause of polyhydramnios, megalencephaly, and symptomatic epilepsy syndrome (PMSE) [MIM:611087]. Affected children have large heads, infantile-onset intractable multifocal seizures and severe psychomotor retardation. Neuropathological studies reveal megalencephaly, ventriculomegaly, cytomegaly and extensive vacuolization and astrocytosis of white matter. Ref.7
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Chromosomal rearrangement Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	activation of protein kinase activity Ref.4 Inferred from direct assay. Source: UniProtKB cell cycle Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Ref.4 Inferred from electronic annotation. Source: InterPro protein export from nucleus Ref.4 Inferred from direct assay. Source: UniProtKB regulation of fatty acid oxidation Inferred from Experiment. Source: Reactome
Cellular component	cytoplasm Ref.4 Inferred from direct assay. Source: UniProtKB nucleus Ref.4 Inferred from direct assay. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.4 Inferred from physical interaction. Source: IntAct protein kinase activity Ref.4 Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
STK11	Q15831	3	EBI-1109114,EBI-306838

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.4 (identifier: Q7RTN6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.2 Ref.3 (identifier: Q7RTN6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     5-41: Missing.
     368-417: PSASTLLNHSFFKQIKRRASEALPELLRPVTPITNFEGSQSQDHSGIFGL → YPCWPGPGLRESRGCSGG
     418-431: Missing.

Isoform 3 Ref.3 (identifier: Q7RTN6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     5-41: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	STE20-related kinase adapter protein alpha		PRO_0000260035
Regions
	Domain	69 – 379	311	Protein kinase
Amino acid modifications
	Modified residue	329	1	Phosphothreonine; by STK11 Ref.4
	Modified residue	419	1	Phosphothreonine; by STK11 Ref.4
Natural variations
	Alternative sequence	5 – 41	37	Missing in isoform 2 and isoform 3. Ref.2 Ref.3		VSP_052219
	Alternative sequence	368 – 417	50	PSAST…GIFGL → YPCWPGPGLRESRGCSGG in isoform 2. Ref.3		VSP_052220
	Alternative sequence	418 – 431	14	Missing in isoform 2. Ref.3		VSP_052221
	Natural variant	13	1	R → W		VAR_041377
	Natural variant	60	1	S → I		VAR_041378
	Natural variant	64	1	P → S		VAR_041379
Experimental info
	Mutagenesis	329	1	T → A: Loss of STK11-mediated phosphorylation. Ref.4
	Mutagenesis	419	1	T → A: Loss of STK11-mediated phosphorylation. Ref.4
	Sequence conflict	339	1	S → W in AAG48269. Ref.1
	Sequence conflict	363	1	N → H in BAC11349. Ref.3
	Sequence conflict	388	1	E → K in AAG48269. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 9CC0A78D9CC6FC2C	FASTA	431	48,369
	10 20 30 40 50 60 MSFLVSKPER IRRWVSEKFI VEGLRDLELF GEQPPGDTRR KTNDASSESI ASFSKQEVMS 70 80 90 100 110 120 SFLPEGGCYE LLTVIGKGFE DLMTVNLARY KPTGEYVTVR RINLEACSNE MVTFLQGELH 130 140 150 160 170 180 VSKLFNHPNI VPYRATFIAD NELWVVTSFM AYGSAKDLIC THFMDGMNEL AIAYILQGVL 190 200 210 220 230 240 KALDYIHHMG YVHRSVKASH ILISVDGKVY LSGLRSNLSM ISHGQRQRVV HDFPKYSVKV 250 260 270 280 290 300 LPWLSPEVLQ QNLQGYDAKS DIYSVGITAC ELANGHVPFK DMPATQMLLE KLNGTVPCLL 310 320 330 340 350 360 DTSTIPAEEL TMSPSRSVAN SGLSDSLTTS TPRPSNGDSP SHPYHRTFSP HFHHFVEQCL 370 380 390 400 410 420 QRNPDARPSA STLLNHSFFK QIKRRASEAL PELLRPVTPI TNFEGSQSQD HSGIFGLVTN 430 LEELEVDDWE F
	Isoform 2. Checksum: B4A9F1EDE0E86EF4	FASTA	348	38,597
	10 20 30 40 50 60 MSFLTNDASS ESIASFSKQE VMSSFLPEGG CYELLTVIGK GFEDLMTVNL ARYKPTGEYV 70 80 90 100 110 120 TVRRINLEAC SNEMVTFLQG ELHVSKLFNH PNIVPYRATF IADNELWVVT SFMAYGSAKD 130 140 150 160 170 180 LICTHFMDGM NELAIAYILQ GVLKALDYIH HMGYVHRSVK ASHILISVDG KVYLSGLRSN 190 200 210 220 230 240 LSMISHGQRQ RVVHDFPKYS VKVLPWLSPE VLQQNLQGYD AKSDIYSVGI TACELANGHV 250 260 270 280 290 300 PFKDMPATQM LLEKLNGTVP CLLDTSTIPA EELTMSPSRS VANSGLSDSL TTSTPRPSNG 310 320 330 340 DSPSHPYHRT FSPHFHHFVE QCLQRNPDAR YPCWPGPGLR ESRGCSGG
	Isoform 3. Checksum: 35C80CA906403ADC	FASTA	394	43,962
	10 20 30 40 50 60 MSFLTNDASS ESIASFSKQE VMSSFLPEGG CYELLTVIGK GFEDLMTVNL ARYKPTGEYV 70 80 90 100 110 120 TVRRINLEAC SNEMVTFLQG ELHVSKLFNH PNIVPYRATF IADNELWVVT SFMAYGSAKD 130 140 150 160 170 180 LICTHFMDGM NELAIAYILQ GVLKALDYIH HMGYVHRSVK ASHILISVDG KVYLSGLRSN 190 200 210 220 230 240 LSMISHGQRQ RVVHDFPKYS VKVLPWLSPE VLQQNLQGYD AKSDIYSVGI TACELANGHV 250 260 270 280 290 300 PFKDMPATQM LLEKLNGTVP CLLDTSTIPA EELTMSPSRS VANSGLSDSL TTSTPRPSNG 310 320 330 340 350 360 DSPSHPYHRT FSPHFHHFVE QCLQRNPDAR PSASTLLNHS FFKQIKRRAS EALPELLRPV 370 380 390 TPITNFEGSQ SQDHSGIFGL VTNLEELEVD DWEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Humoral immunity to human breast cancer: antigen definition and quantitative analysis of mRNA expression." Scanlan M.J., Gout I., Gordon C.M., Williamson B., Stockert E., Gure A.O., Jaeger D., Chen Y.-T., Mackay A., O'Hare M.J., Old L.J. Cancer Immun. 1:4-4(2001) [PubMed: 12747765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Mammary tumor.
[2]	"Cloning, characterization and localization of lyk5 gene." Shan Y.X., Huang C.Q., Yu L. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Tissue: Teratocarcinoma.
[4]	"Activation of the tumour suppressor kinase LKB1 by the STE20-like pseudokinase STRAD." Baas A.F., Boudeau J., Sapkota G.P., Smit L., Medema R., Morrice N.A., Alessi D.R., Clevers H.C. EMBO J. 22:3062-3072(2003) [PubMed: 12805220] [Abstract] Cited for: IDENTIFICATION (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH STK11, MUTAGENESIS OF THR-329 AND THR-419, PHOSPHORYLATION AT THR-329 AND THR-419.
[5]	"MO25alpha/beta interact with STRADalpha/beta enhancing their ability to bind, activate and localize LKB1 in the cytoplasm." Boudeau J., Baas A.F., Deak M., Morrice N.A., Kieloch A., Schutkowski M., Prescott A.R., Clevers H.C., Alessi D.R. EMBO J. 22:5102-5114(2003) [PubMed: 14517248] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH STK11 AND CAB39.
[6]	"Crystal structure of MO25 alpha in complex with the C-terminus of the pseudo kinase STE20-related adaptor." Milburn C.C., Boudeau J., Deak M., Alessi D.R., van Aalten D.M. Nat. Struct. Mol. Biol. 11:193-200(2004) [PubMed: 14730349] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) IN COMPLEX WITH CAB39.
[7]	"Polyhydramnios, megalencephaly and symptomatic epilepsy caused by a homozygous 7-kilobase deletion in LYK5." Puffenberger E.G., Strauss K.A., Ramsey K.E., Craig D.W., Stephan D.A., Robinson D.L., Hendrickson C.L., Gottlieb S., Ramsay D.A., Siu V.M., Heuer G.G., Crino P.B., Morton D.H. Brain 130:1929-1941(2007) [PubMed: 17522105] [Abstract] Cited for: INVOLVEMENT IN PMSE.
[8]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] TRP-13; ILE-60 AND SER-64.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF308302 mRNA. Translation: AAG48269.1.
AY290821 mRNA. Translation: AAP42280.1.
AK074771 mRNA. Translation: BAC11197.1.
AK075005 mRNA. Translation: BAC11349.1.
BK001542 mRNA. Translation: DAA01797.1.

IPI

IPI00300700.
IPI00396083.
IPI00456673.

RefSeq

NP_001003786.1.
NP_001003787.1.
NP_001003788.1.
NP_699166.2.

UniGene

Hs.514402

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1UPK	X-ray	1.85	B	420-431	[»]
3GNI	X-ray	2.35	B	59-431	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q7RTN6. 1 interaction.

STRING

Q7RTN6.

PTM databases

PhosphoSite

Q7RTN6.

Proteomic databases

PRIDE

Q7RTN6.

Genome annotation databases

Ensembl

ENST00000245865; ENSP00000245865; ENSG00000125695; Homo sapiens. [Genome view]
ENST00000336174; ENSP00000336655; ENSG00000125695; Homo sapiens. [Genome view]
ENST00000375840; ENSP00000365000; ENSG00000125695; Homo sapiens. [Genome view]
ENST00000375841; ENSP00000365001; ENSG00000125695; Homo sapiens. [Genome view]
ENST00000392950; ENSP00000376677; ENSG00000125695; Homo sapiens. [Genome view]
ENST00000447001; ENSP00000398841; ENSG00000125695; Homo sapiens. [Genome view]

GeneID

92335.

KEGG

hsa:92335.

UCSC

uc002jbm.1. human.
uc002jbp.1. human.

Organism-specific databases

CTD

92335.

GeneCards

GC17M059134.

HGNC

HGNC:30172. STRADA.

MIM

608626. gene.
611087. phenotype.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

Q7RTN6.

OMA

NGTVPCL.

Enzyme and pathway databases

Reactome

REACT_1505. Integration of energy metabolism.

Gene expression databases

ArrayExpress

Q7RTN6.

Bgee

Q7RTN6.

CleanEx

HS_STRADA.

Genevestigator

Q7RTN6.

GermOnline

ENSG00000125695. Homo sapiens.

Family and domain databases

InterPro

IPR000719. Prot_kinase_core.
IPR017442. Se/Thr_pkinase-rel.
[Graphical view]

Pfam

PF00069. Pkinase. 1 hit.
[Graphical view]

ProDom

PD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

77700.

SOURCE

Search...

Entry information

Entry name

STRAA_HUMAN

Accession

Primary (citable) accession number: Q7RTN6
Secondary accession number(s): Q7Z4K9 , Q8NC31, Q8NCF1, Q9H272

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	December 15, 2003
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.