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Protein

Enolase

Gene

ENO

Organism
Plasmodium yoelii yoelii
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (PY03280)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Enolase (ENO)
  5. Pyruvate kinase (PY04645)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei164SubstrateBy similarity1
Binding sitei173SubstrateBy similarity1
Active sitei216Proton donorBy similarity1
Metal bindingi251MagnesiumBy similarity1
Metal bindingi302MagnesiumBy similarity1
Binding sitei302SubstrateBy similarity1
Metal bindingi329MagnesiumBy similarity1
Binding sitei329SubstrateBy similarity1
Active sitei354Proton acceptorBy similarity1
Binding sitei405SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BRENDAi4.2.1.11. 4895.
UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Gene namesi
Name:ENO
ORF Names:PY06644
OrganismiPlasmodium yoelii yoelii
Taxonomic identifieri73239 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiidaePlasmodiumPlasmodium (Vinckeia)
Proteomesi
  • UP000008553 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340921 – 444EnolaseAdd BLAST444

Proteomic databases

PRIDEiQ7RA60.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi352914.XP_727327.1.

Structurei

3D structure databases

ProteinModelPortaliQ7RA60.
SMRiQ7RA60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni381 – 384Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

eggNOGiKOG2670. Eukaryota.
COG0148. LUCA.
InParanoidiQ7RA60.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7RA60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIINPKNYEH IFYSRGNPTV EVDLETTLGI FRAAVPSGAS TGIYEALELR
60 70 80 90 100
DNDKSRYLGK GVQQAIKNIN EIIAPKLIGL DCREQKKIDN MMVQELDGSK
110 120 130 140 150
TEWGWSKSKL GANAILAISM AICRAGAAAN KTSLYKYVAQ LAGKNTEKMI
160 170 180 190 200
LPVPCLNVIN GGSHAGNKLS FQEFMIVPVG APSFKEAMRY GAEVYHTLKS
210 220 230 240 250
EIKKKYGIDA TNVGDEGGFA PNILNAHEAL DLLVASIKKA GYENKVKIAM
260 270 280 290 300
DVAASEFYNS ETKTYDLDFK TPNNDKSLVK TGQELVDLYI ELVKKYPIIS
310 320 330 340 350
IEDPFDQDDW ENYAKLTEAI GKDVQIVGDD LLVTNPTRIE KALEKKACNA
360 370 380 390 400
LLLKVNQIGS ITEAIEACLL SQKNNWGVMV SHRSGETEDV FIADLVVALR
410 420 430 440
TGQIKTGAPC RSERNAKYNQ LFRIEESLGA NGSFAGDKFR LQLN
Length:444
Mass (Da):48,916
Last modified:July 19, 2004 - v2
Checksum:i5E9803FBAC6364AB
GO

Sequence cautioni

The sequence EAA18892 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABL01002274 Genomic DNA. Translation: EAA18892.1. Different initiation.

Genome annotation databases

EnsemblProtistsiEAA18892; EAA18892; EAA18892.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiENO_PLAYO
AccessioniPrimary (citable) accession number: Q7RA60
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: April 12, 2017
This is version 72 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families