Q7QDU4 (STAT_ANOGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal transducer and transcription activator

Alternative name(s):
Ag-STAT

Gene names

Name:	Stat
ORF Names:	AGAP010423

Organism

Anopheles gambiae (African malaria mosquito) [Reference proteome]

Taxonomic identifier

7165 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicimorpha › Culicoidea › Culicidae › Anophelinae › Anopheles › Cellia › Pyretophorus › gambiae species complex

Protein attributes

Sequence length	722 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Signal transduction and activation of transcription. Plays an important role in the segmental pattern formation in the early embryo by activating specific stripes of pair rule gene expression By similarity. The STAT pathway directly participates in immune responses in insects. Ref.1 UniProtKB Q24151
Subunit structure	Forms a homodimer or a heterodimer with a related family member By similarity. UniProtKB P51692
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: Translocated into the nucleus in response to phosphorylation By similarity.
Tissue specificity	Expressed in hemocytes, pericardial cells, midgut, skeletal muscle and fat body cells. Ref.1
Developmental stage	Expressed at all developmental stages. Ref.1
Induction	Bacterial challenge results in nuclear translocation in fat body cells and induction of DNA-binding activity that recognizes a STAT target site. In vitro treatment with pervanadate (vanadate and H2O2) translocates protein to the nucleus in midgut epithelial cells. Ref.1
Sequence similarities	Belongs to the transcription factor STAT family. Contains 1 SH2 domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Domain	SH2 domain
Ligand	DNA-binding
Molecular function	Activator Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 722	722	Signal transducer and transcription activator		PRO_0000233907
Regions
	Domain	545 – 644	100	SH2
Experimental info
	Sequence conflict	155	1	Q → H in CAA09070. Ref.1
	Sequence conflict	176	1	N → Y in CAA09070. Ref.1
	Sequence conflict	311	1	T → S in CAA09070. Ref.1
	Sequence conflict	547	1	D → N in CAA09070. Ref.1
	Sequence conflict	684	1	T → A in CAA09070. Ref.1
	Sequence conflict	687 – 689	3	NDM → KDT in CAA09070. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q7QDU4 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: BB106241A28F36CC	FASTA	722	83,879
	10 20 30 40 50 60 METRLHQLPP CILEQFHFLN DLKYPVLIRQ HLGNWIKDSL HNAPTYTNNM QSMYELDAAK 70 80 90 100 110 120 FFTALVNEVD QVSANLPNKR KCLLCRSAIM LRDQNFQNLT QLYLTLLHQI QPNCEKGCKT 130 140 150 160 170 180 EYTIAQTSSD GQQTDVLYGL QQLHVMERNN WKETQQLIQE CEQDHVQRLS NQRSHNKRIQ 190 200 210 220 230 240 CYSLKQRSLV DAFQKTIRKA EEVLNLVYNK YIFEWQKTQM FPEVRSTNAY SLDEIQTWYE 250 260 270 280 290 300 SLAAIMWNTK DQIHLTMKSQ LREHVSQEIN SDLWKVMKDV KDFIKLLLHK AFIVENQPPQ 310 320 330 340 350 360 VMKMNTRFCA TVRLLIDNAL IMKIGNPKVT VSIISETQAQ QIQSTNAAAD FSAGEIENNI 370 380 390 400 410 420 GNLQYQLSNK FLANFSNMRL KKINRGNRKL NKLVVDEKFA LLFQSSFTLE QEELTVTVWT 430 440 450 460 470 480 LSLPAVVIVH VNQEQLAWTT IIWDNLCAKA DRKLFEVPNL IPWNRLVEAI SMTFSARVGR 490 500 510 520 530 540 GLTDENMQYM YRKAYRDKLS FSVSNDQMIS FAQFCKDTTP ECNYTFWEWL YAALKIIRDH 550 560 570 580 590 600 LQVLWVDNTI IGFIHKSTAE KYLAKCVPGT FLLRFTDSVL GGISIAWVHE SNDGQRQVLH 610 620 630 640 650 660 IQPFTAKDLV VRSLANRICD LGELTYLYPT IPKQEAFGRY TAPAIQKPRS KHYISAEMRT 670 680 690 700 710 720 VLIFAPSSNQ SSSSTPNAEQ SPSTSSNDMF SNEYVLTNLD EIYKFEIEND DMLSIQDYWE QQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Anopheles gambiae Ag-STAT, a new insect member of the STAT family is activated in response to bacterial infection." Barillas-Mury C., Han Y.-S., Seeley D., Kafatos F.C. EMBO J. 18:959-967(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION. Strain: G3.
[2]	"The genome sequence of the malaria mosquito Anopheles gambiae." Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F. , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L. Science 298:129-149(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PEST.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ010299 Genomic DNA. Translation: CAA09070.1. AAAB01008849 Genomic DNA. Translation: EAA07203.1.
RefSeq	XP_311525.1. XM_311525.1.
3D structure databases
ProteinModelPortal	Q7QDU4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	AGAP010423-RA; AGAP010423-PA; AGAP010423.
GeneID	1272625.
KEGG	aga:AgaP_AGAP010423.
VectorBase	AGAP010424. Anopheles gambiae.
Organism-specific databases
CTD	1272625.
Phylogenomic databases
eggNOG	NOG245085.
HOGENOM	HOG000230988.
InParanoid	Q7QDU4.
OMA	FATTERN.
OrthoDB	EOG4RR4ZC.
PhylomeDB	Q7QDU4.
Gene expression databases
ArrayExpress	Q7QDU4.
Family and domain databases
Gene3D	1.10.238.10. 1 hit. 1.10.532.10. 1 hit. 1.20.1050.20. 1 hit. 2.60.40.630. 1 hit. 3.30.505.10. 1 hit.
InterPro	IPR011992. EF-hand-like_dom. IPR008967. p53-like_TF_DNA-bd. IPR000980. SH2. IPR013800. STAT_TF_alpha. IPR015988. STAT_TF_coiled-coil. IPR001217. STAT_TF_core. IPR013801. STAT_TF_DNA-bd. IPR012345. STAT_TF_DNA-bd_sub. IPR013799. STAT_TF_prot_interaction. [Graphical view]
PANTHER	PTHR11801. PTHR11801. 1 hit.
Pfam	PF00017. SH2. 1 hit. PF01017. STAT_alpha. 1 hit. PF02864. STAT_bind. 1 hit. [Graphical view]
SMART	SM00252. SH2. 1 hit. [Graphical view]
SUPFAM	SSF49417. P53_like_DNA_bnd. 1 hit. SSF47655. STAT. 1 hit.
PROSITE	PS50001. SH2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

STAT_ANOGA

Accession

Primary (citable) accession number: Q7QDU4
Secondary accession number(s): O97164

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	December 15, 2003
Last modified:	April 3, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)