Reviewed, UniProtKB/Swiss-Prot Q7Q6A7 (KMO_ANOGA)

Names and origin

Protein names

Recommended name:
    Kynurenine 3-monooxygenase
    EC=1.14.13.9
Alternative name(s):
    Kynurenine 3-hydroxylase

Gene names

Name:	kh
ORF Names:	AGAP005948

Organism

Anopheles gambiae (African malaria mosquito) [Complete proteome]

Taxonomic identifier

7165 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicoidea › Culicidae › Anophelinae › Anopheles

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid By similarity.
Catalytic activity	L-kynurenine + NADPH + O2 = 3-hydroxy-L-kynurenine + NADP+ + H2O.
Cofactor	FAD By similarity.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3.
Subcellular location	Mitochondrion By similarity. Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the aromatic-ring hydroxylase family. KMO subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Membrane Mitochondrion
Domain	Transmembrane
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD metabolic process Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	kynurenine 3-monooxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	Kynurenine 3-monooxygenase		PRO_0000361913
Regions
	Transmembrane	401 – 424	24	Potential
	Transmembrane	437 – 461	25	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7Q6A7-1 [UniParc]. Last modified December 7, 2004. Version 2. Checksum: 5A5F80A34C8FAB97	FASTA	486	55,010
	10 20 30 40 50 60 MATASDSKYK RTNTNGMQHQ PLDVAIVGGG LVGSLLALHL GKKGHEVNLY EYREDIRTAE 70 80 90 100 110 120 LVIGRSINLA LSARGRRALA EVGLEDALLN HGIPMSGRML HDVNGKCKIV PYDANTNQCI 130 140 150 160 170 180 YSVGRKHLNE VLLNAAEKYP NIHLHFNHKL VSANLDEGNL SMVDPVTKDV KSARADLIVG 190 200 210 220 230 240 CDGAYSAVRK EIVKRPRYDF SQTYIEHGYL ELCIPPTAGG EFAMPHNYLH IWPRGQFMMI 250 260 270 280 290 300 ALPNQDRTWT VTLFMPFTQF HSITDQGLLL DFFRQHFPDA IELIGRERLV KDFFKTKAQP 310 320 330 340 350 360 LVMIKCRPYH IGAKALIIGD AAHAMVPFYG QGMNAGFEDC SVLTELFNQY GTDLARILPE 370 380 390 400 410 420 FSEKRWEDAH AICDLAMYNY IEMRDLVTKR SYLLRKKLDE LLFWMMPNTW VPLYNSVSFS 430 440 450 460 470 480 HMRYSKCIAN RAWQDKILTR VLYGASIASV AAIGGLCYRH VTMGHLERLS TRILSTFQLL KPKASV

References

[1]

"The genome sequence of the malaria mosquito Anopheles gambiae." Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F. , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L. Science 298:129-149(2002) [PubMed: 12364791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PEST.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAAB01008960 Genomic DNA. Translation: EAA11712.2.
RefSeq	XP_315983.2.
3D structure databases
SMR	Q7Q6A7. Positions 22-397.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7Q6A7.
Genome annotation databases
GeneID	1276617.
KEGG	aga:AgaP_AGAP005948.
VectorBase	AGAP005948. Anopheles gambiae.
Phylogenomic databases
eggNOG	inNOG08413.
HOGENOM	HBG430104.
OMA	HWQKKVI.
OrthoDB	EOG96149V.
PhylomeDB	Q7Q6A7.
Family and domain databases
InterPro	IPR002938. mOase_FAD_bd. IPR003042. Rng_hydrolase-like. [Graphical view]
Pfam	PF01494. FAD_binding_3. 1 hit. [Graphical view]
PRINTS	PR00420. RNGMNOXGNASE.
ProtoNet	Search...

Entry information

Entry name

KMO_ANOGA

Accession

Primary (citable) accession number: Q7Q6A7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	December 7, 2004
Last modified:	January 19, 2010
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)